Close Relationship between cIAP2 and Human ARDS Induced by Severe H7N9 Infection.

BACKGROUND: cIAP2 is involved in necroptosis as a key upstream regulation factor. We aimed to investigate the role of cIAP2 in ARDS/ALI induced by H7N9 virus through regulating the RIPK1/3 necroptosis pathway. METHODS: Lung tissues of 11 patients who died from ARDS-complicated H7N9 infection between 2013 and 2016 were obtained as the H7N9-ARDS group. Lung tissues near benign lung nodules were acquired as the control group. Histological changes were evaluated by H&E staining. Protein levels of cIAP2, RIPK1, RIPK3, p-RIPK3, MLKL, and p-MLKL in the lung tissues were detected by Western Blot. The mRNA levels of cIAP2, RIPK1, and RIPK3 were detected by real-time PCR. RESULTS: H7N9 virus infection had a high mortality, with ARDS being the leading cause of death. The protein level of cIAP2 in the experimental group was lower than that in the control group (P<0.05). However, the experimental group showed higher RIPK1, RIPK3, and p-RIPK3 protein levels than the control group (P<0.05), as well as the expression level of MLKL and p-MLKL protein, which is a key downstream protein in necroptosis (P<0.05). CONCLUSION: In tissues from patients with fatal H7N9, downregulation of cIAP2 and induction of necroptosis was observed. We could speculate that necroptosis of the pulmonary epithelium is associated with severe H7N9 infection leading to ARDS. Thus, necroptosis inhibition may be a novel therapy for H7N9 influenza virus.

A dodecanuclear heterometallic dysprosium-cobalt wheel exhibiting single-molecule magnet behaviour.

A novel dodecanuclear wheel with ten Dy(III) ions and two Co(II) ions bridged by four Schiff-base ligands and sixteen acetates represents the highest-nuclearity 3d-4f example of its type displaying single-molecule magnet behaviour.

(2-Oxido-1-naphthaldehyde benzoyl-hydrazonato-κN,N',O)pyridine-copper(II).

In the mononuclear title compound, [Cu(II)(C(18)H(12)N(2)O(2))(C(5)H(5)N)], the Cu(II) ion is coordinated by two O atoms and one N atom from the dianionic tridentate L(2-) ligand (H(2)L is 2-hy-droxy-1-naphthaldehyde benzoyl-hydrazide) and one N atom from a pyridine mol-ecule in a CuN(2)O(2) distorted square-planar coordination environment.

Bis[µ-2-(2-benzoyl-hydrazinylidenemeth-yl)-6-methoxy-phenolato][2-(2-benzoyl-hydrazinylidenemeth-yl)-6-methoxy-phenolato]dimanganese(II) perchlorate methanol solvate.

In the title complex, [Mn(2)(C(15)H(13)N(2)O(3))(3)]ClO(4)·CH(3)OH, the two Mn(II) ions are bridged by two phenolate O atoms from two ligands, forming an Mn(2)O(2) quadrangle. Each Mn(II) ion has a distorted octa-hedral coordination geometry. One Mn(II) ion is coordinated by two N atoms and four O atoms from two ligands, and the other is coordinated by one N atom and five O atoms from three ligands. A dimer is formed by inter-molecular N-H⋯O hydrogen bonds. The dimers, perchlorate anions and methanol solvent mol-ecules are further connected into a chain along [01] through N-H⋯O and O-H⋯O hydrogen bonds.

Bis{2-[(2-benzoyl-hydrazin-1-yl-idene)meth-yl]-6-meth-oxy-phenolato}iron(III) chloride monohydrate.

In the title mononuclear iron(III) complex, [Fe(C(15)H(13)N(2)O(3))(2)]Cl·H(2)O, the Fe(III) atom has a distorted octa-hedral geometry and is six-coordinated by four O atoms and two N atoms from two ligands. In the crystal structure, the complex cations, Cl(-) anions and water mol-ecules are connected into a chain along [100] through N-H⋯O, O-H⋯Cl and N-H⋯Cl hydrogen bonds. Two adjacent chains are linked by O-H⋯O hydrogen bonds.