The structural stability, electronic properties regulation and feasibility of controllable preparation of a C0.5/(BN)0.5 heterojunction single-walled nanotube.

Our work investigates the structural stability of a C0.5/(BN)0.5 heterojunction single-walled nanotube by comparing the binding energy. The energy band structure, electronic density of states and regulation relation between band gap and indirect-direct properties and tube diameter and type are systematically studied. Based on existing experimental and theoretical results, dynamic simulated calculating of the stitching process is carried out to explore the feasibility of controllable preparation.

Influence of energy loss function to the Monte Carlo simulated electron backscattering coefficient.

We report an improved calculation of the electron backscattering coefficients (BSCs) for beryllium, molybdenum and tungsten at electron energies of 0.1-100 keV based on an up-to-date Monte Carlo simulation method with different input of energy loss function (ELF) data. The electron inelastic cross-section is derived from the relativistic dielectric functional formalism, where the full Penn's algorithm is applied for the extension of the ELF from the optical limit of [Formula: see text] into the [Formula: see text]-plane. We have found that the accuracy of energy loss function may affect largely the calculated BSC. We also show that this has close relationship with the f- and ps-sum rules.

Adipose specific aptamer adipo-8 recognizes and interacts with APMAP to ameliorates fat deposition in vitro and in vivo.

AIMS: To identify the target of an adipose specific aptamer adipo-8, predict the potential interaction between adipo-8 and its target, and investigate lipid-lowering effect of adipo-8 in vitro and in vivo. MAIN METHODS: Distinct membranous protein of 3T3-L1 adipocyte pulled-down by adipo-8 was mass-spectrometry analyzed as target candidate(s), and affinity of adipo-8 to target protein-silent adipocyte was detected to validate it. Interaction between adipo-8 and target was predicted by bioinformatic analysis, further confirmed by aptamer truncation and competitive binding assay. To investigate lipid-lowering effect of adipo-8 and mechanism behind, 250 nmol/L adipo-8 or library was incubated with 3T3-L1 adipocyte or target-protein-silent adipocyte for 24 h, and 0.01 µg/g/day adipo-8 or library was administrated to high-fat-fed male mice for 21 days. KEY FINDINGS: APMAP (Adipocyte Plasma Membrane Associated Protein) was identified as adipo-8 target, and adipo-8 affinity to adipocytes was in proportional to APMAP expression. Docking model between the stem-loop structure of adipo-8 and APMAP were predicted that adipo-8 was likely to interact with APMAP at its amino-acid 275-411 sequence. Moreover, adipo-8 could ameliorate fat deposition through interaction with APMAP in vitro, and administration of adipo-8 in high-fat-diet fed mice resulted in body weight loss and blood triglyceride decrease without liver or renal dysfunction. SIGNIFICANCE: Adipo-8 could recognize APMAP specifically and interact with its targets to ameliorate fat deposition in vitro and in vivo. Aptamer adipo-8 has potential to act as an effective and safe targeted drug for obesity and obesity related diseases.

[Drinking in male HBV carriers in the outpatient department].

OBJECTIVE: To understand the relation among drinking, characteristics and emotion in outpatient male carriers with hepatitis B virus (HBV), and to provide reference for alcohol intervention. METHODS: We used alcohol use disorders identification test (AUDIT), self-rating anxiety scale (SAS), and Beck depression inventory (BDI) to investigate 980 male HBV carriers in the outpatient department. RESULTS: The questionnaires were responded by 544 people with drinking experience for nearly a year (drinking rate 58.18%). The prevalence of moderate drinking was 37.8% (354 patients), hazardous and harmful drinking 17.97% (168 patients) and alcohol dependence 2.35% (22 patients). In groups with different ages, education levels, occupations, income and symptoms, the constituent ratio of the hazardous harmful drinking and alcohol dependence (AUDIT 7-26 scores) was different (P<0.05). The total score in SAS in the alcohol dependence group was much higher than that in the normal group (35.95±11.55 vs 29.78±0.46, P =0.020); the total score in SAS and in BDI was significantly higher in the alcohol dependence group than that in the moderate drinking group and the hazardous harmful drinking group (35.95±11.55 and 10.45±8.95 vs 29.65±7.97 and 6.35±5.65 vs 29.68±7.06 and 6.44±5.27, respectively). CONCLUSION: Male HBV carriers of 30-49 years old, with education level lower than elementary school or higher than bachelor degree, cadres/professionals with high income and major symptoms show higher harmful/alcohol dependence (AUDIT 7-26 points). Anxiety and depression in the alcohol-dependent male hepatitis B virus carriers are obviously higher than in the moderate drinking group and the hazardous harmful drinking group.