The A subunit of vacuolar H+-ATPase gene (CmVHA-A) plays opposite roles in plant growth and drought tolerance of chrysanthemum under different growing conditions.

As the most prominent proton pumps in plants, vacuolar H+-ATPases (VHAs) comprise multiple subunits that are important for physiological processes and stress tolerance in plants. However, few studies on the roles of subunit genes of VHAs in chrysanthemum have been reported to date. In this study, the gene of A subunit of V-ATPase in chrysanthemum (CmVHA-A) was cloned and identified. CmVHA-A was conserved with VHA-A proteins from other plants. Expression analysis showed that CmVHA-A was highly expressed in most tissues of chrysanthemum except for the flower bud, and was readily induced by polyethylene glycol (PEG) treatment. Functional analysis demonstrated that CmVHA-A exerted a negative influence on the growth and development of shoot and root of chrysanthemum under normal conditions. RNA-sequencing (RNA-seq) analysis revealed the possible explanations for phenotypic differences between transgenic and wild-type (WT) plants. Under drought conditions, CmVHA-A positively affected the drought tolerance of chrysanthemum by enhancing antioxidase activity and alleviating photosynthetic disruption. Overall, CmVHA-A plays opposite roles in plant growth and drought tolerance of chrysanthemums under different growing conditions.

Bone remodelling prediction using mechanical stimulus with bone connectivity theory in porous implants.

Strain energy density (SED) is considered to be the primary remodelling stimulus influencing the process of bone growth into porous implants. A bone remodelling algorithm incorporating the concept of bone connectivity, that newly formed bone should only grow from existing bone, was developed to provide a more biologically realistic simulation of bone growth. Results showed that the new algorithm prevented the occurrence of unconnected mature bone within porous implants, an unrealistic phenomenon observed using conventional adaptive elasticity theories. The bone connectivity algorithm had minimal effect (0.67% difference) on the final bone density distribution for standard bending and torsional moment cases. For a porous implant model, both algorithms, with and without bone connectivity implementation, reached the same final stiffness, with a difference of less than 0.01%. The bone connectivity algorithm predicted a slower and more gradual bone remodelling process, requiring at least 50% additional time for full remodelling compared to the conventional adaptive elasticity algorithm, which should be accounted for in the planning of rehabilitation strategies. The developed modelling can be employed to improve porous implant designs to achieve better clinical outcomes.

Biochar changed the distribution of imidacloprid in a plant-soil-groundwater system.

The use of biochar has increased, as its physicochemical properties reduce the adverse effects of pesticides. However, few studies have comprehensively investigated the effects of biochar on the distribution of pesticides in a plant-soil-groundwater system. In this study, a biochar produced from rice straw at 550 °C was chosen, and column experiments with five rated of biochar application (application rates = 0.0, 1.0, 2.0, 3.0, and 4.0% w/w for B0-B4, respectively) were conducted to investigate the capacity of biochar to immobilize imidacloprid (IMI) in soil, thereby decreasing its uptake by plants and leaching from soil into groundwater. Our results showed that IMI in plants, leached from soil, and detected in soil accounted for 3.78, 1.76, and 36.4% of the total IMI input, respectively, and the biochar treatments dramatically decreased the IMI distribution to 0.57, 0.11, and 13.4%, respectively. By contrast, the percentage of undetected IMI increased from 58.1% in the B0 treatment to an average of 86.0% in the biochar treatments. Biochar treatments increased IMI immobilization in soil, which could be related to the increased soil carbon content, surface area, cation exchange capacity. This study indicates that biochar with characters of high surface area and porosity can stabilize IMI and reduce its potential to harm plants and groundwater.