RESUMO
Human American trypanosomiasis is resurgent in Latin Americans, and new drugs are urgently required as current medications suffer from a number of drawbacks. Some nitroheterocycles have been demonstrated to exert a potent activity against trypanosomes. However, host toxicity issues halted their development as trypanocides. As part of the efforts to develop new compounds in order to treat parasitic infections, it is important to define their structure-activity relationship. In this study, 5-nitromegazol and two of its analogues, 4-nitromegazol, and 1-methyl-5-nitro-2-imidazolecarboxaldehyde 5-nitroimidazole-thiosemicarbazone, were tested and compared for in vitro induction of DNA damage in human leukocytes by the comet assay, performed at different pHs to better identify the types of damage. Specific oxidatively generated damage to DNA was also measured by using the comet assay with endonucleases. DNA damage was found in 5-nitromegazol-treated cells: oxidative stress appeared as the main source of DNA damage. 4-Nitromegazol did not produce any significant effect, thus confirming that 4-nitroimidazoles isomers have no important biological activity. The 5-nitroimidazole-thiosemicarbazone induced DNA damage with a higher efficiency than 5-nitromegazol. The central role in the reduction process played by the acidic hydrazine proton present in the thiosemicarbazone group but not in the cyclic (thiadiazole) form can contribute to rationalise our results. Given its versatility, thiosemicarbazone moiety could be involved in different reactions with nitrogenous bases (nucleophilic and/or electrophilic attacks).
Assuntos
Dano ao DNA/efeitos dos fármacos , Fragmentação do DNA/efeitos dos fármacos , Leucócitos/efeitos dos fármacos , Nitroimidazóis/farmacologia , Tripanossomicidas/farmacologia , Sobrevivência Celular/efeitos dos fármacos , Sobrevivência Celular/fisiologia , Humanos , Leucócitos/citologiaRESUMO
Cyto- and genotoxicity induced by drugs can limit the dose and duration of treatment, can adversely affect patient quality of life, and may be life-threatening. Two drugs are currently being used for treatment of the acute phase of Chagas' disease and both have serious undesirable effects. In this research, cyto- and genotoxic activity of the nitroimidazole-tiadiazole derivative CL 64855 2-amino-5-(1-methyl-5-nitro-2-imidazolyl)-1,3,4-thiadiazole (megazol), a promising alternative drug, was evaluated in vitro with different short-term tests: (a) induction of recombination events and mutation in the yeast Saccharomyces cerevisiae D7 strain, with and without induction of cytochrome P-450; DNA damage (single and double strand breaks, alkali-labile sites, etc.) by the Comet assay in different mammalian cells. S. cerevisiae did not show a significant increase of mutant and recombinant event frequency, both with and without cytochrome P-450. On the other hand, the cytochrome complex appeared to detoxify the drug with respect to cytotoxicity. Results in rat and mouse fresh leukocytes showed a dose-response relation of drug-induced DNA damage. Findings in treated VERO cells suggested a complex treatment time-DNA damage relationship and the possible induction of repair mechanisms. Furthermore, bleomycin effects were increased in rat cells by simultaneous administration of megazol. Megazol shows different biological activity in relation to cellular types and experimental conditions (with or without cytochrome P-450, short/long time of exposure, with or without other genotoxins), thus suggesting a modulation of effectiveness by different physiological/biochemical conditions of cells. The findings could be useful to evaluate new megazol-derived compounds and to assess the risks/benefits relationship for each drug.
Assuntos
Dano ao DNA/efeitos dos fármacos , Leucócitos/efeitos dos fármacos , Tiadiazóis/farmacologia , Tripanossomicidas/farmacologia , Animais , Sobrevivência Celular/efeitos dos fármacos , Doença de Chagas/tratamento farmacológico , Chlorocebus aethiops , Relação Dose-Resposta a Droga , Leucócitos/patologia , Camundongos , Ratos , Trypanosoma cruzi/efeitos dos fármacos , Trypanosoma cruzi/genética , Células Vero , Leveduras/efeitos dos fármacosRESUMO
This paper presents a new method for detecting mitotic crossing-over in Aspergillus nidulans, based on the "homozygosity index" (HI) of recessive genes originally present in hetrozygosis in diploid strains, which occurs after mitotic crossing-over between the marker in question and the centromere. Since homozygous diploids (-/-) for auxotrophic markers can not grow in MM, homozygotization can be demonstrated by distorted mitotic segregation of the alleles involved. Two similar diploid strains (UT 448/UT 184 and Z1//UT 184), which differ by a chromosomic duplicate segment transposed from chromosome II to I in the Z1 haploid strain, were used. This excess of genetic material confers to the Z1 mutant the uvs character and makes Z1//UT 184 more unstable and sensitive to genotoxic agents, as evidenced by its high spontaneous recombinational index.