[Identification of microorganisms based on the effect of giant Raman scattering].

AIM: Development and evaluation of express diagnostics of microorganisms for reduction of time spent on study execution. MATERIALS AND METHODS: Giant Raman scattering effect and proposed substrate with metallic nano-balls of silver were used for identification and indication of microorganisms. Staphylococcus aureus, Staphylococcus haemolyticus, Staphylococcus saprophyticus, Candida albicans, Escherichia coli, Bacillus subtillis, Pseudomonas aeruginosa were used as objects of study. All the microorganisms were cultivated in dense nutrient media and non-fluorescent microbiological membrane filters. RESULTS: Giant Raman scattering method on the substrates used increases sensitivity of indication and specificity of identification of microbes compared with other existing express methods and allows to see the signal from a single bacterium when exposed to laser light. The methodic proposed was also used to determine sensitivity of bacteria to antimicrobial preparations. CONCLUSION: The proposed hardware and method may be used for express diagnostics of processes of microbial nature.

[Experience in the use of Romashka-2 device in gynecologic laser microsurgery]. / Opyt primeneniia v ginekologii lazernoi mikrokhirurgicheskoi ustanovki "Romashka-2".

The outfit Romashka-2 intended for evaporation, coagulation and preparation of thin biostructures in the superficial and deep operative fields by focused radiation of CO2 laser is widely used mostly in gynecology, otolaryngology and other branches of medicine. Experience gained with the 4-years' clinical trial of the outfit, carried out at one of the women's consultation clinics permitted one to assess the Soviet public health requirement of the outfits Romashka-2 as well as to calculate the economic effect provided by its use.