Diurnal trends of indoor and outdoor fluorescent biological aerosol particles in a tropical urban area.

We evaluated diurnal trends of size-resolved indoor and outdoor fluorescent biological airborne particles (FBAPs) and their contributions to particulate matter (PM) within 0.5-20 µm. After a ten-week continuous sampling via two identical wideband integrated bioaerosol sensors, we found that both indoor and outdoor diurnal trends of PM were driven by its bioaerosol component. Outdoors, the median [interquartile range] FBAP mass concentration peaked at 8.2 [5.8-9.9] µg/m3 around sunrise and showed a downtrend from 6:00 to 18:00 during the daytime and an uptrend during the night. The nighttime FBAP level was 1.8 [1.4-2.2] times higher than that during the daytime, and FBAPs accounted for 45 % and 56 % of PM during daytime and nighttime, respectively. Indoors, the rise in concentrations of FBAPs smaller than 1 µm coincided with the starting operation of the heating, ventilation, and air conditioning (HVAC) system at 6:00, and the concentration peaked at 8:00 and dropped to the daily average by noontime. This indicated that the starting operation of the HVAC system dislodged the overnight settled and accumulated fine bioaerosols into the indoor environment. For particles larger than 1 µm, the variation of mass concentration was driven by occupancy. Based on regression modeling, the contributions of indoor PM, non-FBAP, and FBAP sources to indoor mass concentrations were estimated to be 93 %, 67 %, and 97 % during the occupied period.

Size-resolved dynamics of indoor and outdoor fluorescent biological aerosol particles in a bedroom: A one-month case study in Singapore.

This study evaluated the interrelations between indoor and outdoor bioaerosols in a bedroom under a living condition. Two wideband integrated bioaerosol sensors were utilized to measure indoor and outdoor particulate matter (PM) and fluorescent biological airborne particles (FBAPs), which were within a size range of 0.5-20 µm. Throughout this one-month case study, the median proportion of FBAPs in PM by number was 19% (5%; the interquartile range, hereafter) and 17% (3%) for indoors and outdoors, respectively, and those by mass were 78% (12%) and 55% (9%). According to the size-resolved data, FBAPs dominated above 2 and 3.5 µm indoors and outdoors, respectively. Comparing indoor upon outdoor ratios among occupancy and window conditions, the indoor FBAPs larger than 3.16 µm were dominated by indoor sources, while non-FBAPs were mainly from outdoors. The occupant dominated the indoor source of both FBAPs and non-FBAPs. Under awake and asleep, count- and mass-based mean emission rates were 45.9 and 18.7 × 106 #/h and 5.02 and 2.83 mg/h, respectively. Based on indoor activities and local outdoor air quality in Singapore, this study recommended opening the window when awake and closing it during sleep to lower indoor bioaerosol exposure.