Assuntos
Exaustão por Calor , Peçonhas/toxicidade , Animais , Relação Dose-Resposta a Droga , Temperatura Alta , RatosRESUMO
The rigidity of neutrophilic granulocyte membrane was determined: 2.1+/-0.7 kPa. Scanning in the contact mode did not modify cell morphology, while spectroscopy caused nonspecific swelling reaction and a 3-fold increase in cell volume. Spectroscopy had no effect on rigidity of neutrophilic granulocyte membrane.
Assuntos
Membrana Celular/ultraestrutura , Microscopia de Varredura por Sonda/métodos , Neutrófilos/citologia , Elasticidade , Humanos , Análise EspectralRESUMO
Morphological parameters of human blood neutrophilic granulocytes were studied by scanning probe microscopy. The basic morphological characteristics of the living (unfixed) and fixed cells were compared. It was found that using the method described, the dimensions of the cells changed substantially and significantly depending upon the mode of fixation applied. The methanol fixation allowed to demonstrate the internal structure of a cell, though it sharply deformed the object of study. After glutaraldehyde fixation cells possessed the morphological characteristics similar to those of the living cells, but the diameter and height of the fixed cells were again different from those of living cells. The resolution of a scanning probe microscope was higher when the fixed cells were studied, permitting the visualization of a detailed cell structure, including the nucleus and cytoplasmic granules (in particular, when methanol fixation was used). However, this method of study is unreliable for the determination of cell dimensions (diameter and height).
