RESUMO
The recently developed QBC technique has been reported to be rapid, sensitive and specific for the detection of malaria infection. We evaluated this technique in comparison with thin and thick Giemsa stained blood films for the diagnosis of malaria in acute fever and PUO cases. The technique was slightly more sensitive than thin blood film but equal to thick blood film examination. Identification of species of malaria posed some difficulties. Due to its high cost and requirement for a fluorescent microscope this technique, in spite of its speed and sensitivity, may not have the potential to be a substitute for the conventional blood film examination for the diagnosis of malaria, especially in developing countries.
Assuntos
Laranja de Acridina , Malária Falciparum/diagnóstico , Malária Vivax/diagnóstico , Animais , Corantes Azur , Países em Desenvolvimento , Estudos de Avaliação como Assunto , Humanos , Microscopia de Fluorescência , Plasmodium falciparum/isolamento & purificação , Plasmodium vivax/isolamento & purificação , Kit de Reagentes para Diagnóstico , Sensibilidade e Especificidade , Coloração e Rotulagem , IêmenRESUMO
A cross-sectional survey of 348 subjects without evidence of liver disease was conducted to investigate the prevalence and risk factors for hepatitis C virus antibody (anti-HCV) seropositivity in the Yemen Arab Republic. The mean age of study subjects was 28.7 years (range 3-80), and 61% were males. Using commercial enzyme-linked immunosorbent assays (ELISA), 6.0% (95% confidence interval [CI] 3.8-9.1) of subjects were anti-HCV-positive, 13.5% were hepatitis B surface antigen-positive (HBsAg-positive), and 51.4% were positive for at least one serologic marker of prior hepatitis B infection. Nine (2.6%; 95% CI 1.2-4.9) of the 21 ELISA-positive sera were confirmed to be anti-HCV positive by a recombinant immunoblot assay. Anti-HCV seropositivity was significantly associated with age (odds ratio [OR] 2.0 for each 10-year increase in age) and prior surgery (OR 10.1), but was not associated with a history of prior blood transfusion or markers of hepatitis B infection. These preliminary data suggest that hepatitis C may pose a substantial health threat in Yemen.
Assuntos
Hepacivirus/imunologia , Anticorpos Anti-Hepatite/sangue , Hepatite C/epidemiologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Pré-Escolar , Estudos Transversais , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Immunoblotting , Masculino , Pessoa de Meia-Idade , Prevalência , Fatores de Risco , Estudos Soroepidemiológicos , Iêmen/epidemiologiaRESUMO
During February 1988 a seroepidemiological survey of hepatitis A, B and D was performed in the Yemen Arab Republic. 879 sera were collected from 4 different areas; Sanaa, Hajja, Hodeidah and Taiz. The prevalence of hepatitis B surface antigen (HBsAg) was 12.7% (112/879) and some marker of hepatitis B infection was found in 45.5% (399/879) of study subjects. Only 2 (1.8%) of the 112 HBsAg positives were positive for antibody to delta hepatitis, and 9.7% (9/93) were positive for hepatitis B e antigen (HBeAg). Univariate analysis showed age, sex, qat chewing, blood transfusion, surgery and a past history of jaundice to be associated with hepatitis B infection. Using multivariate logistic regression analysis only, age (odds ratios 1.37 for HBsAg carriers and 1.51 for seropositives), a past history of jaundice (odds ratio 1.42), and combined history of blood transfusion and surgery (odds ratio 2.76) were independent predictors of infection. Hepatitis B appears to be a major health concern in the Yemen Arab Republic.
