[The characteristics of formation of antibodies to xeno- and endobiotics in women with inherent malformation of fetus].

The study revealed high levels of antibodies class A and G to benzopyrene and antibodies class G to estradiol and progesterone in women with pathology of fetus development. The most informative indicators of high risk of development of reproductive pathology are established The recommendations concerning the application of antibodies to benzopyrene, estradiol and progesterone in diagnostics of inherent malformations of fetus are provided.

Immunological images of polycyclic aromatic hydrocarbons.

AIM: To develop experimental model for definition of immunological images of chemical carcinogens. MATERIALS AND METHODS: The conjugates of benzo[a]pyrene, benz[a]anthracene, anthracene, chrysene and pyrene with bovine serum albumin and yeast hexokinase were synthesized. Rabbits were immunized by bovine serum albumin-hapten conjugates. Antibodies to each hapten were isolated from the serum by affinity chromatography with the hapten-yeast hexokinase-Sepharose sorbents. The binding of each hapten with each antibody was determined by competitive immunoassay. RESULTS: The immunological images of all the investigated chemical compounds were described. CONCLUSION: The model is proposed to determine the internal immunological images of anti-idiotypic monoclonal antibodies to chemical carcinogens.

Are There Any Interrelations Between Immunogenic Activity of Chemical Carcinogens and Tumour Antigens in Cancer Patients?

Some interrelations between generation of antibodies to polycyclic aromatic hydrocarbons and immunogenic capacity of carcinoembryonic antigen were studied in patients with mammary and gastrointestinal types of cancer. IgM, IgG and IgA antibodies to polycyclic aromatic hydrocarbons were determined by a method of enzyme-linked immunosorbent assay. Immunogenic activity of carcinoembryonic antigen was evaluated by its ability to form circulating immune complexes. It was found that in patients with mammary cancer, generations of IgA to polycyclic aromatic hydrocarbons was interrelated with high immunogenic form of carcinoembryonic antigen, whereas IgG synthesis was associated with its low immunogenic form. Alternatively, patients with gastro-intestinal cancer did not exhibit any appreciable interrelation between generation of immunoglobulins and immunogenic activity of carcinoembryonic antigen.

[New approaches in evaluating the antitumor immune response in breast cancer]. / Novye podkhody k otsenke protivoopukholevoi immunoreaktivnosti pri rake molochnoi zhelezy.

Different classes of antibodies to polycyclic aromatic hydrocarbons (PAH) and immunogenicity of carcinoembryonic antigen (CEA) were investigated in breast cancer patients versus course. Prior to treatment, the levels of IgM, IgG and IgA antibodies to PAHs were 5.9, 7.7 and 32.5%, respectively. In cases of tumor regression, they were 13.8, 25.0 and 19.2%, while in those of progression-2.4, 2.8 and 12.8%, respectively. The immunogenicity of CEA, i.e. the power of forming circulating immune complexes, appeared to be higher in cases of tumor regression than in those of progression. CEA concentration in immune complexes exceeding 5ng/ml was observed in 24.0 and 15.1% of cases, respectively, while in whole serum, the concentration in excess of 10 ng/ml was in 8.0 and 42.4%, respectively.