SPOP promotes SIRT2 degradation and suppresses non-small cell lung cancer cell growth.

SIRT2 is a NAD-dependent deacetylase and inhibition of SIRT2 has a broad anticancer activity. Here we report that SPOP binds to SIRT2 and mediates its degradation by the 26S proteasome, which can be blocked by MG132 treatment. We also found that the levels of SPOP significantly decreased, while the levels of SIRT2 significantly increased in non-small cell lung cancer (NSCLC) cell lines, compared to normal bronchial epithelial cell line and NSCLC specimens, compared to the paired non-tumor lung tissue. Furthermore, SPOP can suppress NSCLC cell growth. Notably, mutations in NSCLC inhibit the abilities of SPOP to degrade SIRT2 and suppress NSCLC cell growth. These results reveal a novel regulation of SIRT2 by SPOP mediated degradation, which is important for the growth of lung tumor cells.

Study on expression of Syntaxin 8 protein in gastric carcinoma / 中国现代普通外科进展

Objective:To determine the expression of syntaxin 8 in gastric carcinoma and its clinical significance.Methods:To determine the expression levels of syntaxin 8 in gastric carcinoma and pericancerous stomach tissues of 62 patients with gastric carcinoma by immunohistochemistry,western blot.Results:Results of immunohistochemistry and western blot showed that the expression of syntaxin 8 was significantly higher than that in the adjacent pericancerous stomach tissues,and syntaxin 8 is negative in most normal tissues.The difference was statistically significant (P＜0.05).Correlation analysis found that the high expression of syntaxin 8 in gastric carcinoma tissue was not significantly related to gender,age,tumor size,TNM stage,invasion and metastasis of gastric carcinoma,but the higher expression rate was up-regulated in gastric carcinoma with distant metastasis.Conclusion:Syntaxin 8 is abnormal high expression in gastric carcinoma tissues,and is related to the occurrence and development of gastric carcinoma.It may also be a potential target for gastric carcinoma diagnosis and treatment.

Compatibility law of Schisandra chinensis prescription of Chinese patent medicine in Chinese pharmacopoeia / 中国药理学通报

Aim To summarize the 2015 version of the prescrip-tion preparations containing Schisandra chinensis in "Chinese Pharmacopoeia",and discover meaningful compatibility law of Schisandra chinensis. Methods The statistics of prescription preparations relating to Schisandra chinensis in 2015 edition of "Chinese Pharmacopoeia" were gathered to find the frequency of each herbal medicine,efficacy,medicine category,and dosage. A database was constructed and frequency statistics were coun-ted,the dendrogram was built by cluster analysis to get compati-bility law of herbal medicines,and then some modern research analysis was conducted. Results A total 100 kinds of prescrip-tion preparations were included,including 128 Chinese medi-cines,and the total using frequency was 906 times. A sum of 17 types of traditional Chinese medicine were used in this study, and most commonly used herbals were Qi-invigorating herbs(fre-quency 150,0. 1656),antipyretic(frequency 115,0. 1269), yin-nourishing herbs(frequency 91,0. 1004),Yang-invigorat-ing herbs(frequency 78,0. 8610),Hematinic(frequency 78, 0. 8610)and so on. The herb medicine of using frequency ranking the first 20 was Prepared Radix Rehmanniae(47),Ra-dix Ophiopogonis (39 ), tuckahoe (38 ), Membranous Milkvetch(36),Ural Licorice (35),ginseng (32),angelica (23),salvia miltiorrhiza(23),wolfberry fruit(21),respective-ly;cluster analysis showed that the traditional Chinese medicine with frequency more than 10 times could be divided into five cat-egories,while Radix Rehmanniae was classified as a separate class. Conclusions The main therapeutic theory about Schisan-dra is astringency,replenishing and promoting production of body fluid and tonifying the kidney to relieve mental strain in 2015 edition of "Chinese Pharmacopoeia". The prescription compatibility can be used for rational medicine Schisandra com-patibility,guiding rational administration for Schisandra chinen-sis,in-depth research and development of modern prescription provide reference.

Study on expression of Syntaxin 8 protein in gastric carcinoma / 中国现代普通外科进展

Objective:To determine the expression of syntaxin 8 in gastric carcinoma and its clinical significance.Methods:To determine the expression levels of syntaxin 8 in gastric carcinoma and pericancerous stomach tissues of 62 patients with gastric carcinoma by immunohistochemistry,western blot.Results:Results of immunohistochemistry and western blot showed that the expression of syntaxin 8 was significantly higher than that in the adjacent pericancerous stomach tissues,and syntaxin 8 is negative in most normal tissues.The difference was statistically significant (P＜0.05).Correlation analysis found that the high expression of syntaxin 8 in gastric carcinoma tissue was not significantly related to gender,age,tumor size,TNM stage,invasion and metastasis of gastric carcinoma,but the higher expression rate was up-regulated in gastric carcinoma with distant metastasis.Conclusion:Syntaxin 8 is abnormal high expression in gastric carcinoma tissues,and is related to the occurrence and development of gastric carcinoma.It may also be a potential target for gastric carcinoma diagnosis and treatment.

Study on influence of buffer system on separate capacity of macroporous resin on ferulic liposome / 中国中药杂志

<p><b>OBJECTIVE</b>To study the effect of buffer on separate capacity of macroporous resin. To evaluate the quality of ferulic acid liposome and determine its entrapment efficiency.</p><p><b>METHOD</b>Different type of macroporous resin counterpoised by buffer system of Na2 HPO3-NaH2, PO3 was used to separate the free ferulic acid from the preparation and HPLC was used to determine the concentration of the ferulic acid to calculate the entrapment efficiency.</p><p><b>RESULT</b>This method had good linearity in the range of 0.56 - 2.8 g x mL(-1) (r = 0.999 6). The precision RSD was less than 1.1%. The adsorption effect of macroporous resin on liposome was reduced while it had no effect on the absorption ability of macroporous resin on the ferulic acid by the usage of buffer. The recovery of HPD450 resin on blank liposome was between 97.2% - 100.8%, while the average recovery is 98.1%.</p><p><b>CONCLUSION</b>Buffer system can enhance the separate ability of macroporous resin on liposome and free drug.</p>