In Silico and In Vitro Evaluation of the Antifungal Activity of a New Chromone Derivative against Candida spp.

Candida species are frequently implicated in the development of both superficial and invasive fungal infections, which can impact vital organs. In the quest for novel strategies to combat fungal infections, there has been growing interest in exploring synthetic and semi-synthetic products, particularly chromone derivatives, renowned for their antimicrobial properties. In the analysis of the antifungal activity of the compound (E)-benzylidene-chroman-4-one against Candida, in silico and laboratory tests were performed to predict possible mechanisms of action pathways, and in vitro tests were performed to determine antifungal activity (MIC and MFC), to verify potential modes of action on the fungal cell membrane and wall, and to assess cytotoxicity in human keratinocytes. The tested compound exhibited predicted affinity for all fungal targets, with the highest predicted affinity observed for thymidylate synthase (-102.589 kJ/mol). MIC and CFM values ranged from 264.52 µM (62.5 µg/mL) to 4232.44 µM (1000 µg/mL). The antifungal effect likely occurs due to the action of the compound on the plasma membrane. Therefore, (E)-benzylidene-chroman-4-one showed fungicidal-like activity against Candida spp., possibly targeting the plasma membrane.

Anticonvulsant activity of Tetrahydrolinalool: behavioral, electrophysiological, and molecular docking approaches.

Tetrahydrolinalool (THL) is an acyclic monoterpene alcohol, produced during linalol metabolism and also a constituent of essential oils. As described in the literature, many monoterpenes present anticonvulsant properties, and thus we became interested in evaluating the anticonvulsant activity of Tetrahydrolinalool using in mice model as well as in silico approaches. Our results demonstrated that THL increased latency to seizure onset and also reduced the mortality, in picrotoxin induced seizure tests. The results may be related to GABAergic regulation, which was also suggested in seizure testing induced by 3-mercapto-propionic acid. In the strychnine-induced seizure testing, none of the groups pretreated with THL modulated the parameters indicative of anticonvulsant effect. The electrophysiological results revealed that THL treatment reduces seizures induced by pentylenetetrazole. The in silico molecular docking studies showed that the interaction between THL and a GABAA receptor model formed a stable complex, in comparison to the crystaligraphic structure of diazepam, a structurally related ligand. In conclusion, all the evidences showed that THL presents effective anticonvulsant activity related to the GABAergic pathway, being a candidate for treatment of epileptic syndromes.

An In Silico Approach to Exploring the Antinociceptive Biological Activities of Linalool and its Metabolites.

Pain is characterized by the unpleasant sensory and emotional sensation associated with actual or potential tissue damage, whereas nociception refers to the mechanism by which noxious stimuli are transmitted from the periphery to the CNS. The main drugs used to treat pain are nonsteroidal anti-inflammatory drugs (NSAIDs) and opioid analgesics, which have side effects that limit their use. Therefore, in the search for new drugs with potential antinociceptive effects, essential oils have been studied, whose constituents (monoterpenes) are emerging as a new therapeutic possibility. Among them, linalool and its metabolites stand out. The present study aims to investigate the antinociceptive potential of linalool and its metabolites through a screening using an in silico approach. Molecular docking was used to evaluate possible interactions with important targets involved in antinociceptive activity, such as α2-adrenergic, GABAergic, muscarinic, opioid, adenosinergic, transient potential, and glutamatergic receptors. The compounds in the investigated series obtained negative energies for all enzymes, representing satisfactory interactions with the targets and highlighting the multi-target potential of the L4 metabolite. Linalool and its metabolites have a high likelihood of modulatory activity against the targets involved in nociception and are potential candidates for future drugs.

Mechanisms Involved in the Therapeutic Effect of Cannabinoid Compounds on Gliomas: A Review with Experimental Approach.

INTRODUCTION: Brain tumors have high morbidity and mortality rates, accounting for 1.4% of all cancers. Gliomas are the most common primary brain tumors in adults. Currently, several therapeutic approaches are used; however, they are associated with side effects that affect patients'quality of life. Therefore, further studies are needed to develop novel therapeutic protocols with a more favorable side effect profile. In this context, cannabinoid compounds may serve as potential alternatives. OBJECTIVE: This study aimed to review the key enzymatic targets involved in glioma pathophysiology and evaluate the potential interaction of these targets with four cannabinoid derivatives through molecular docking simulations. METHODS: Molecular docking simulations were performed using four cannabinoid compounds and six molecular targets associated with glioma pathophysiology. RESULTS: Encouraging interactions between the selected enzymes and glioma-related targets were observed, suggesting their potential activity through these pathways. In particular, cannabigerol showed promising interactions with epidermal growth factor receptors and phosphatidylinositol 3- kinase, while Δ-9-tetrahydrocannabinol showed remarkable interactions with telomerase reverse transcriptase. CONCLUSION: The evaluated compounds exhibited favorable interactions with the analyzed enzymatic targets, thus representing potential candidates for further in vitro and in vivo studies.

Biochemical aspects of the inflammatory process: A narrative review.

Inflammation is a protective response of the body potentially caused by microbial, viral, or fungal infections, tissue damage, or even autoimmune reactions. The cardinal signs of inflammation are consequences of immunological, biochemical, and physiological changes that trigger the release of pro-inflammatory chemical mediators at the local of the injured site thus, increasing blood flow, vascular permeability, and leukocyte recruitment. The aim of this study is to give an overview of the inflammatory process, focusing on chemical mediators. The literature review was based on a search of journals published between the years 2009 and 2023, regarding the role of major chemical mediators in the inflammatory process and current studies in pathogenesis, diagnosis, and therapy. Some of the recent contributions in the study of inflammatory pathologies and their mediators, including cytokines and chemokines, the kinin system, free radicals, nitric oxide, histamine, cell adhesion molecules, leukotrienes, prostaglandins and the complement system and their role in human health and chronic diseases.

Proposition of In silico Pharmacophore Models for Malaria: A Review.

In the field of medicinal chemistry, the concept of pharmacophore refers to the specific region of a molecule that possesses essential structural and chemical characteristics for binding to a receptor and eliciting biological activity. Understanding the pharmacophore is crucial for drug research and development, as it allows the design of new drugs. Malaria, a widespread disease, is commonly treated with chloroquine and artemisinin, but the emergence of parasite resistance limits their effectiveness. This study aims to explore computer simulations to discover a specific pharmacophore for Malaria, providing new alternatives for its treatment. A literature review was conducted, encompassing articles proposing a pharmacophore for Malaria, gathered from the "Web of Science" database, with a focus on recent publications to ensure up-to-date analysis. The selected articles employed diverse methods, including ligand-based and structurebased approaches, integrating molecular structure and biological activity data to yield comprehensive analyses. Affinity evaluation between the proposed pharmacophore and the target receptor involved calculating free energy to quantify their interaction. Multiple linear regression was commonly utilized, though it is sensitive to multicollinearity issues. Another recurrent methodology was the use of the Schrödinger package, employing tools such as the Phase module and the OPLS force field for interaction analysis. Pharmacophore model proposition allows threedimensional representations guiding the synthesis and design of new biologically active compounds, offering a promising avenue for discovering therapeutic agents to combat Malaria.

Anti-Inflammatory Activity of N'-(3-(1H-indol-3-yl)benzylidene)-2-cyanoacetohydrazide Derivative via sGC-NO/Cytokine Pathway.

The N-acylhydrazone function has been reported as a pharmacophore group of molecules with diverse pharmacological activities, including anti-inflammatory effects. Therefore, this study was designed to evaluate the anti-inflammatory potential of the compound N'-(3-(1H-indol-3-yl)benzylidene)-2-cyanoacetohydrazide (JR19) in vivo. The study started with the carrageenan-induced peritonitis model, followed by an investigation of leukocyte migration using the subcutaneous air pouch test and an assessment of the antinociceptive profile using formalin-induced pain. A preliminary molecular docking study focusing on the crystallographic structures of NFκB, iNOS, and sGC was performed to determine the likely mechanism of action. The computational study revealed satisfactory interaction energies with the selected targets, and the same peritonitis model was used to validate the involvement of the nitric oxide pathway and cytokine expression in the peritoneal exudate of mice pretreated with L-NAME or methylene blue. In the peritonitis assay, JR19 (10 and 20 mg/kg) reduced leukocyte migration by 59% and 52%, respectively, compared to the vehicle group, with the 10 mg/kg dose used in subsequent assays. In the subcutaneous air pouch assay, the reduction in cell migration was 66%, and the response to intraplantar formalin was reduced by 39%, particularly during the inflammatory phase, suggesting that the compound lacks central analgesic activity. In addition, a reversal of the anti-inflammatory effect was observed in mice pretreated with L-NAME or methylene blue, indicating the involvement of iNOS and sGC in the anti-inflammatory response of JR19. The compound effectively and significantly decreased the levels of IL-6, TNF-α, IL-17, and IFN-γ, and this effect was reversed in animals pretreated with L-NAME, supporting a NO-dependent anti-inflammatory effect. In contrast, pretreatment with methylene blue only reversed the reduction in TNF-α levels. Therefore, these results demonstrate the pharmacological potential of the novel N-acylhydrazone derivative, which acts through the nitric oxide pathway and cytokine signaling, making it a strong candidate as an anti-inflammatory and immunomodulatory agent.

ACW-02 an Acridine Triazolidine Derivative Presents Antileishmanial Activity Mediated by DNA Interaction and Immunomodulation.

The present study proposed the synthesis of a novel acridine derivative not yet described in the literature, chemical characterization by NMR, MS, and IR, followed by investigations of its antileishmanial potential. In vitro assays were performed to assess its antileishmanial activity against L. amazonensis strains and cytotoxicity against macrophages through MTT assay and annexin V-FITC/PI, and the ability to perform an immunomodulatory action using CBA. To investigate possible molecular targets, its interaction with DNA in vitro and in silico targets were evaluated. As results, the compound showed good antileishmanial activity, with IC50 of 6.57 (amastigotes) and 94.97 (promastigotes) µg mL-1, associated with non-cytotoxicity to macrophages (CC50 > 256.00 µg mL-1). When assessed by flow cytometry, 99.8% of macrophages remained viable. The compound induced an antileishmanial effect in infected macrophages and altered TNF-α, IL-10 and IL-6 expression, suggesting a slight immunomodulatory activity. DNA assay showed an interaction with the minor grooves due to the hyperchromic effect of 47.53% and Kb 1.17 × 106 M-1, and was sustained by docking studies. Molecular dynamics simulations and MM-PBSA calculations propose cysteine protease B as a possible target. Therefore, this study demonstrates that the new compound is a promising molecule and contributes as a model for future works.

Computational Studies Applied to Linalool and Citronellal Derivatives Against Alzheimer's and Parkinson's Disorders: A Review with Experimental Approach.

Alzheimer's and Parkinson's are neurodegenerative disorders that affect a great number of people around the world, seriously compromising the quality of life of individuals, due to motor and cognitive damage. In these diseases, pharmacological treatment is used only to alleviate symptoms. This emphasizes the need to discover alternative molecules for use in prevention. Using Molecular Docking, this review aimed to evaluate the anti-Alzheimer's and anti-Parkinson's activity of linalool and citronellal, as well as their derivatives. Before performing Molecular Docking simulations, the compounds' pharmacokinetic characteristics were evaluated. For Molecular Docking, 7 chemical compounds derived from citronellal, and 10 compounds derived from linalool, and molecular targets involved in Alzheimer's and Parkinson's pathophysiology were selected. According to the Lipinski rules, the compounds under study presented good oral absorption and bioavailability. For toxicity, some tissue irritability was observed. For Parkinson-related targets, the citronellal and linalool derived compounds revealed excellent energetic affinity for α-Synuclein, Adenosine Receptors, Monoamine Oxidase (MAO), and Dopamine D1 receptor proteins. For Alzheimer disease targets, only linalool and its derivatives presented promise against BACE enzyme activity. The compounds studied presented high probability of modulatory activity against the disease targets under study, and are potential candidates for future drugs.

Anxiolytic and Antidepressant-like Effects of Monoterpene Tetrahydrolinalool and In silico Approach of new Potential Targets.

INTRODUCTION: Although drugs currently available for the treatment of anxiety and depression act through modulation of the neurotransmission systems involved in the neurobiology of the disorder, yet they often present side effects, which can impair patient adherence to treatment. METHODS: This has driven the search for new molecules with anxiolytic and antidepressant potential. Aromatic plants are rich in essential oils, and their chemical constituents, such as monoterpenes, are being studied for these disorders. This study aims to evaluate the anxiolytic and antidepressant-like potential of the monoterpene tetrahydrolinalool in in vivo animal models and review pharmacological targets with validation through molecular docking. Male Swiss mice (Mus musculus) were treated with THL (37.5-600 mg kg-1 p.o.) and submitted to the elevated plus maze, open field, rotarod, and forced swim tests. In the elevated plus-maze, THL at doses of 37.5 and 75 mg kg-1 induced a significant increase in the percentage of entries (72.7 and 64.3% respectively), and lengths of stay (80.3 and 76.8% respectively) in the open arms tests. RESULTS: These doses did not compromise locomotor activity or motor coordination in the animals. In the open field, rotarod tests, and the forced swimming model, treatment with THL significantly reduced immobility times at doses of 150, 300, and 600 mg kg-1, and by respective percentages of 69.3, 60.9 and 68.7%. CONCLUSION: In molecular docking assay, which investigated potential targets, THL presented satisfactory energy values for: nNOs, SGC, IL-6, 5-HT1A, NMDAr, and D1. These demonstrate the potential of THL (a derivative of natural origin) in in vivo and in silico models, making it a drug candidate.

The Compound (E)-2-Cyano-N,3-diphenylacrylamide (JMPR-01): A Potential Drug for Treatment of Inflammatory Diseases.

The compound (E)-2-cyano-N,3-diphenylacrylamide (JMPR-01) was structurally developed using bioisosteric modifications of a hybrid prototype as formed from fragments of indomethacin and paracetamol. Initially, in vitro assays were performed to determine cell viability (in macrophage cultures), and its ability to modulate the synthesis of nitrite and cytokines (IL-1ß and TNFα) in non-cytotoxic concentrations. In vivo, anti-inflammatory activity was explored using the CFA-induced paw edema and zymosan-induced peritonitis models. To investigate possible molecular targets, molecular docking was performed with the following crystallographic structures: LT-A4-H, PDE4B, COX-2, 5-LOX, and iNOS. As results, we observed a significant reduction in the production of nitrite and IL-1ß at all concentrations used, and also for TNFα with JMPR-01 at 50 and 25 µM. The anti-edematogenic activity of JMPR-01 (100 mg/kg) was significant, reducing edema at 2-6 h, similar to the dexamethasone control. In induced peritonitis, JMPR-01 reduced leukocyte migration by 61.8, 68.5, and 90.5% at respective doses of 5, 10, and 50 mg/kg. In silico, JMPR-01 presented satisfactory coupling; mainly with LT-A4-H, PDE4B, and iNOS. These preliminary results demonstrate the strong potential of JMPR-01 to become a drug for the treatment of inflammatory diseases.

Ionic Channels as Potential Targets for the Treatment of Autism Spectrum Disorder: A Review.

Autism spectrum disorder (ASD) is a neurological condition that directly affects brain functions and can culminate in delayed intellectual development, problems in verbal communication, difficulties in social interaction, and stereotyped behaviors. Its etiology reveals a genetic basis that can be strongly influenced by socio-environmental factors. Ion channels controlled by ligand voltage-activated calcium, sodium, and potassium channels may play important roles in modulating sensory and cognitive responses, and their dysfunctions may be closely associated with neurodevelopmental disorders such as ASD. This is due to ionic flow, which is of paramount importance to maintaining physiological conditions in the central nervous system and triggers action potentials, gene expression, and cell signaling. However, since ASD is a multifactorial disease, treatment is directed only to secondary symptoms. Therefore, this research aims to gather evidence concerning the principal pathophysiological mechanisms involving ion channels in order to recognize their importance as therapeutic targets for the treatment of central and secondary ASD symptoms.

Temporal changes in the potential geographic distribution of Histiotus velatus (Chiroptera, Vespertilionidae), the "decade effect".

We investigated how the potential distribution of Histiotus velatus is affected by the addition of new records over decades (decade effect). Assuming that (1: hypothesis of the effect of the decade) the addition of new occurrence records over time increases the potential size of the species distribution; and (2: Wallacean distance hypothesis) over the years, the new points added are increasingly distant from the research centers. Considering the geographic knowledge gap of this species, our objective is to report a new record of this species and estimate its potential distribution in South America through environment niche models (ENMs). For this, we compiled records of occurrence of species, selected from 1900 to 2015. We used 19 bioclimatic variables available in the WorldClim database to estimate the potential distribution of the species, and we used three modeling algorithms: Maximum Entropy (MXT), Random Forest (RDF), and Support Vector Machine. To test the Wallacean distance hypothesis, we calculated the Euclidian distance from occurrences to bat research centers in Brazil, located using a national researchers' information dataset ("Plataforma Lattes"). To test the hypothesis of the decade effect, we used the beta regression analysis, taking conservative and non-conservative approaches. The results showed that the predicted area expanded and retracted with the addition of new occurrences over the decades, with an improvement in the accuracy of models. Most records are located in the southeastern region of Brazil, but algorithms predicted areas in regions where there are no records. Only the conservative approach has had a positive relationship over the decades. The distance from new points does not increase over the years of research centers.

Potentially zoonotic parasites in the soil of public squares in the city of Aracaju (Sergipe, Northeastern Brazil).

This study evaluated the soil contamination of public squares in the city of Aracaju, Sergipe, by potentially zoonotic parasites and correlated their occurrence with climatic variables (temperature, humidity and precipitation). Samples were collected over a 18-month period, from 20 different public squares, and submitted to three different parasitological techniques: Faust's, Hoffman's and Rugai's methods, adapted to soil samples. Results indicated the presence of several potentially zoonotic parasitic species in eighteen of the 20 squares analyzed (90%). The parasites identified included Ancylostoma spp., Strongyloides stercoralis, Toxocara spp., Dipylidium caninum, Trichuris sp., Capillaria sp. and Giardia sp. They were identified during all months of the year and no influence of temperature, humidity or precipitation on the occurrence of those parasites was observed. Such results demonstrate that public squares in the city of Aracaju pose a parasitic contamination risk for people and pets that visit those places as a leisure activity.

A Novel Feature Selection Method for Uncertain Features: An Application to the Prediction of Pro-/Anti-Longevity Genes.

Understanding the ageing process is a very challenging problem for biologists. To help in this task, there has been a growing use of classification methods (from machine learning) to learn models that predict whether a gene influences the process of ageing or promotes longevity. One type of predictive feature often used for learning such classification models is Protein-Protein Interaction (PPI) features. One important property of PPI features is their uncertainty, i.e., a given feature (PPI annotation) is often associated with a confidence score, which is usually ignored by conventional classification methods. Hence, we propose the Lazy Feature Selection for Uncertain Features (LFSUF) method, which is tailored for coping with the uncertainty in PPI confidence scores. In addition, following the lazy learning paradigm, LFSUF selects features for each instance to be classified, making the feature selection process more flexible. We show that our LFSUF method achieves better predictive accuracy when compared to other feature selection methods that either do not explicitly take PPI confidence scores into account or deal with uncertainty globally rather than using a per-instance approach. Also, we interpret the results of the classification process using the features selected by LFSUF, showing that the number of selected features is significantly reduced, assisting the interpretability of the results. The datasets used in the experiments and the program code of the LFSUF method are freely available on the web at http://github.com/pablonsilva/FSforUncertainFeatureSpaces.

Frequency of Screening and Prevalence of Neurosyphilis in Stroke Population.

BACKGROUND AND AIMS: Syphilis and stroke are high prevalent diseases in south Brazil and estimates of concomitance and possible role of syphilis in acute stroke are lacking. Our aims are to estimate the prevalence of syphilis and neurosyphilis (NS) in a cohort of tertiary stroke center. METHODS: We reviewed all hospital records of stroke/transitory ischemic attack (TIA) using International Classification of Diseases, 10th revision, at discharge, frequency of syphilis screen, serology positivity, cerebrospinal fluid (CSF) analysis, and prevalence of NS in this stroke population applying CDC criteria. RESULTS: Between 2015 and 2016, there were 1,436 discharges for cerebrovascular events and in 78% (1,119) of these cases, some syphilis screening was performed. We have found a frequency of positive serology for syphilis of 13% (143/1,119), and higher stroke severity was the main determinant for non-screening. Applying standard NS criteria, 4.7% (53/1,119) cases with CSF analysis had NS diagnosis: 8 based on CSF-Venereal Disease Research Laboratory (VDRL) positive and 45 based on abnormal CSF white cells or protein, but CSF VDRL negative. NS VDRL positive cases were younger, had higher serum VDRL title, had more frequent HIV infection, and received NS treatment more often. Demographic and clinical characteristics were not different between NS VDRL negative and non-NS cases. CONCLUSION: Positive syphilis serology is frequent in patients with acute stroke/TIA in our region. Acute post-stroke CSF abnormalities make the diagnosis of NS difficult in the context of CSF VDRL negative.

Periviabilidad en Uruguay: Conducta perinatal del equipo de salud. Opinión de expertos / Perinatal viability in Uruguay: Perinatal actions by the health team. The opinion of experts

En Uruguay no se conoce la opinión de los profesionales en relación a cuál sería para ellos la edad límite de viabilidad humana ni su conducta en general cuando se enfrentan a prematuros extremos, motivo por el cual se implementó la encuesta. Se realizó un trabajo descriptivo, observacional y de corte transversal. De los 83 encuestados casi 90% o más encuentra muy importante el rol que cumple la familia como participante activo en la toma de decisiones junto al equipo de salud. Las acciones terapéuticas tomadas a favor de proporcionar un tratamiento proactivo para brindarle posibilidades de sobrevida al recién nacido (inicio de corticoides, antibióticos profilácticos, útero-inhibición, monitorización de bienestar fetal, derivación a CTI neonatal, etcétera) se hacen más manifiestas a partir de las 24 semanas de edad gestacional en adelante con un porcentaje mayor a 90% en todas las preguntas referidas. Más de 90% de los médicos neonatólogos y hasta 50% de los médicos ginecólogos no se siente amparado por un marco legal que garantice sus acciones dentro de lo ético y humanamente justificable. Como conclusión de este trabajo las opiniones manifestadas por los profesionales encuestados es que la viabilidad neonatal se encuentra entre las 24 y 26 semanas, diferenciándose con la franja de edades gestacionales que debaten en la actualidad distintas Sociedades Científicas Internacionales en la materia , muchas de las cuales encuentran su límite entre las 23 a 24 semanas.

The opinion of professionals in relation to what they understand the age limit for human viability is, or the actions they take in general, when they face premature ends are not known in Uruguay. For this reason, a survey was implemented to learn about this issue. A descriptive, observational and cross-sectional study was conducted. Almost 90% or even more of the 83 physicians consulted find the role of the family is very important as they are active participants in the making of decisions along with the health team. The therapeutic actions taken geared to providing a proactive treatment to offer the newborn the possibility of survival (initiation of corticoids, prophylactic antibiotics, utero inhibition, monitoring of fetal well-being, and referral to neonatal ICU etc) are more obvious as from 24 weeks of gestational age, being it over 90% in all questions referred. Over 90% of neonatologists and up to 50% of gynecologists believe they are acting without a legal framework that guarantees their actions in terms of what is ethically and humanly justifiable. As a conclusion of this study, the opinions by the professionals surveyed is that neonatal viability is between 24 and 26 weeks, differing from the strip of gestational ages international scientific societies debate nowadays, many of which finds the boundary between 23 to 24 weeks.

Mechanical behavior of zirconia and titanium abutments before and after cyclic load application.

STATEMENT OF PROBLEM: Esthetic factors influence the decision to use titanium or zirconia abutments in anterior regions. Clinicians may have concerns about the durability and behavior of these zirconia abutments. PURPOSE: The purpose of this in vitro study was to evaluate the longitudinal and transverse long axes of the implant-abutment interface before and after the cyclic loading of titanium and zirconia abutments with an external hexagon. MATERIAL AND METHODS: Forty dental implants with an external hexagon and 40 corresponding abutments made of titanium (Ti) and zirconia (Zr) were subjected to cyclic load (c1) versus no load (c2). The longitudinal and transverse axes of 4 experimental groups (Tic1, Tic2, Zrc1, and Zrc2) were analyzed (vertical/horizontal adjustment) using a scanning electron microscope at ×1000 magnification. The differences among the groups were determined by 1-way analysis of variance (ANOVA) and post hoc Tukey tests (α=.05). T tests were used to identify the statistically significant differences between each group and each condition (α=.05). RESULTS: Significant differences were found among the groups with respect to the misfits analyzed in the 2 sections (longitudinal and transverse) before and after load application (P<.05). The behaviors of the groups differed particularly with regard to the accommodation of sets (abutment/implant) after the application of cyclic loads (P<.05). CONCLUSION: The use of zirconia abutments in titanium implants can cause changes to and/or permanent deformation of the implant hexagon.

Cooperação entre agentes de endemias e escolas na identificação e controle da dengue / Cooperation between health personnel and schools for the identification and control of dengue / Cooperación entre agentes de endemias y escuelas en la identificación y control de la dengue

Analisar o processo de cooperação entre os agentes de endemia e a escola em áreas favoráveis à reprodução do vetor da dengue em domicílios. Métodos: A pesquisa, exploratória e descritiva, caracterizou-se por uma abordagem quantitativa e qualitativa. Os dados foram obtidos por meio de três visitas a 93 domicílios de estudantes do ensino fundamental de uma escola pública em Teófilo Otoni-MG. As visitas ocorreram com intervalos de 30 dias, por meio da cooperação entre agentes de endemias e pesquisadores orientados por uma lista de verificação para identificação de possíveis criadouros do mosquito Aedes aegypti. Após a primeira visita, realizou-se uma ação educativa na escola visando fomentar o debate acerca da prevenção da dengue e controle do vetor. Duas visitas posteriores foram realizadas para verificar possíveis mudanças no ambiente domiciliar. Após as visitas, entrevistas com os agentes de endemias buscaram identificar suas percepções quanto à participação no processo de investigação. Resultados: Na primeira visita, em 83 (89,3%) casas havia presença de algum recipiente propício à reprodução do vetor da dengue. Após o desenvolvimento de ações educativas, na segunda e terceira visitas, o número de casas com criadouros potenciais diminuiu para 65 (70,0%) e 63 (68,0%), respectivamente, indicando o potencial dessas atividades para o controle do vetor da doença. Conclusão: O estudo demonstra o potencial de ações educativas de cooperação entre agentes de endemia e escolas do ensino básico,viabilizando a intersetorialidade na prevenção da dengue...

To analyze the process of cooperation between health personnel and the school in potential dengue vector breeding sites in households. Methods: This is an exploratory and descriptive research using a quantitative and qualitative design. Data were obtained in three visits to 93 households of students from a public elementary school in Teófilo Otoni, MG. The visits were performed every 30 days through the cooperation between health personnel and researchers who identified potential Aedes aegypti breeding sites by using a checklist. A health education campaign was held at the school to foster debate on dengue prevention and vector control. Two visits were performed after the campaign to verify possible changes in the students? households. After that, the health personnel were questioned about their perceptions regarding the participation in the investigation process. Results: It could be observed, during the first visit, that 83 (89.3%) households had some kind of container suitable for the dengue vector breeding. During the second and third visits ? after the health education campaign ? the number of households with potential breeding sites decreased to 65 (70%) and 63 (68%) respectively, showing the important role of such campaigns in the vector control. Conclusion: The study shows the power of health education campaigns developed by health personnel in cooperation with elementary public schools to foster intersectoral actions for dengue prevention...

Analizar el proceso de cooperación entre los agentes de endemia y la escuela en áreas favorables a la reproducción del vector de la dengue en domicilios. Métodos: La investigación exploratoria y descriptiva se caracterizó por un abordaje cuantitativo y cualitativo. Los datos fueron obtenidos a través de tres visitas en 93 domicilios de estudiantes de educación primária de una escuela pública en Teófilo Otoni/MG. Las visitas ocurrieron en intervalos de 30 días por medio de la cooperación entre agentes y endemias e investigadores orientados por uma lista de verificación de posibles criaderos del mosquito Aedes aegypti. Tras la primera visita se realizó una acción educativa en la escuela con el objetivo de proporcionar un debate sobre la prevención de la dengue y el control del vector. Dos visitas fueron realizadas después para verificar posibles cambios en El ambiente domiciliario. Después de las visitas, entrevistas con los agentes de endemias buscaron identificar sus percepciones cuanto a la participación en el proceso de investigación. Resultados: En la primera visita, en 83 (89,3%) de las casas había presencia de algún recipiente propicio para la reproducción del vector de dengue. Después del desarrollo de acciones educativas em la segunda y tercera visitas, el número de casas con criaderos potenciales disminuyó respectivamente para 65 (70,0%) y 63 (68,0%) indicando el potencial de estas actividades para el control del vector de la enfermedad. Conclusión: El estudio demuestra el potencial de acciones educativas de cooperación entre agentes de endemia y escuelas de educación primaria viabilizando La intersectorialidad en la prevención de la dengue...