Fluoride ingestion from toothpaste and diet in 1- to 3-year-old Brazilian children.

OBJECTIVE: This study estimated the total daily fluoride intake of 1- to 3-year-old children from diet and dentifrice. The constituents of the diet were divided into solids, water, milk, and other beverages, which were analyzed separately. The correlation between fingernail fluoride concentrations and the total daily fluoride intake by children was also investigated. METHODS: Thirty-three children, living in a fluoridated area, participated in the study. Fluoride intake from diet was monitored by the 'duplicate plate' method, investigating the different constituents of the diet. Fluoride ingested from dentifrice was determined by subtracting the amount of fluoride recovered after brushing from the amount originally placed onto the child's toothbrush. Fingernails were clipped and collected on three occasions. Fluoride was analyzed with the ion-specific electrode, after hexamethyldisiloxane-facilitated diffusion. Data were tested by anova and Tukey's post hoc tests, Student's t-tests and linear regression (P < 0.05). RESULTS: Mean (+/-SD) fluoride intake from diet and dentifrice was 0.025 +/- 0.013 and 0.106 +/- 0.085 mg/kg body weight/day, respectively, totaling 0.130 mg/kg body weight/day. A strong positive correlation (r = 0.971, P < 0.0001) was seen between the amount of dentifrice loaded onto the brush (0.49 +/- 0.30 g) and the amount of fluoride ingested during each tooth brushing (0.59 +/- 0.45 mg). Among the constituents of the diet, water and milk had a significantly higher contribution to the fluoride intake (0.18 +/- 0.11 mg/day, P < 0.0001), when compared with solids (0.07 +/- 0.05 mg/day) and other beverages (0.07 +/- 0.04 mg/day). Mean (+/-SD) fingernail fluoride concentration on the three dates of collection was 3.11 +/- 1.14, 2.22 +/- 1.47 and 3.53 +/- 1.40 mug F/g. There was no significant correlation between fingernail fluoride concentration and the total fluoride intake. CONCLUSIONS: Most of the children are exposed to a daily fluoride intake above the suggested threshold for dental fluorosis. The dentifrice alone is responsible for an average of 81.5% of the daily fluoride intake, while among the constituents of the diet, water and milk are the most important contributors. In addition, small variations in daily fluoride intake cannot be detected in fingernails.

Availability of fluoride from meals given to kindergarten children in Brazil.

OBJECTIVES: The aim of this study was to evaluate the amount of fluoride supplied daily in the meals given in 44 public kindergarten schools in Bauru, Brazil. In addition, the fluoride concentration of water supplies and its impact on the amount of fluoride found in the meal samples were also investigated. METHODS: Meal samples and water were collected during 2 weeks (10 working days) in public kindergarten schools. Samples of meals were homogenized with known volumes of deionized water. Fluoride present in meal samples was analyzed with the ion-specific electrode (Orion 9609), after hexamethyldisilazane-facilitated diffusion. Fluoride in water samples was analyzed with the same electrode, after buffering with TISAB II. All the analyses were made in duplicate. RESULTS: Fluoride analyzed (mean+/-SD) was 0.50+/-0.20 microg/ml, ranging from nondetectable to 1.42 microg/ml for water samples (n=424) and 0.067+/-0.059 mg, ranging from 0.007 to 0.580 mg for meal samples (n=431). A weak but significant correlation was observed between the amount of fluoride in meals and fluoride concentration in water supplies (r=0.139, P=0.0042). CONCLUSIONS: Despite the seemingly small role played by school meals in the total daily fluoride intake, they can contribute to the total fluoride intake of children on a chronic basis, when in association with other fluoride products. Additionally, the impact of fluoridated public water supply on the final fluoride concentration of the school meals analyzed may be regarded as low.

Bone surface and whole bone as biomarkers for acute fluoride exposure.

This study compares fluoride concentrations ([F]) in surface and whole bone for up to 27 days following an acute oral dose of F. Four groups of rats received single oral F dose (50 mg/kg body weight), and the control group received deionized water (n = 10/group). Groups were euthanized at 1, 3, 9, or 27 days after F administration. Plasma and femurs were collected. F on the femur surface was removed from a circular area (4.52 mm(2)) by immersion in 0.5M HCl for 15 s. The solution was buffered with total ionic strength adjustment buffer and analyzed with an electrode. The subjacent bone was sectioned and ashed at 600 degrees C. Ash and plasma were analyzed for F with the electrode following hexamethyldisiloxane-facilitated diffusion. Data were analyzed by Kruskall-Wallis and Dunn's test and by linear regression (p < 0.05). Peak plasma and bone surface [F] occurred on day 1 (0.26 +/- 0.14 microg/mL and 1801 +/- 888 microg/g, respectively). Bone surface [F] at 3, 9, and 27 were not statistically different from control. A significant increase in whole bone [F] was observed 3 days after F administration and the [F] remained relatively constant thereafter. The mean (+/- SD) surface/whole bone [F] ratios for the control and F groups were 2.45 +/- 0.98, 3.92 +/- 1.32, 1.61 +/- 0.82, 1.73 +/- 0.39, and 1.09 +/- 0.28, respectively. Plasma and bone surface [F]s were positively correlated (r = 0.74). Thus, bone surface was found to be a suitable biomarker for acute, sublethal F exposure 1 day after F administration. Whole bone [F] were significantly increased at 3, 9, and 27 days after F administration.

Evaluation of total pH and soluble and ionic fluoride concentrations in dentifrices commercially available in Brazil.

PURPOSE: To verify the total (TF), soluble (SF) and ionic (IF) fluoride concentrations in the main Brazilian dentifrices for comparison and quality control according to the current Brazilian Ministry of Health Regulations, 2000. MATERIALS AND METHODS: Nineteen brands, from 2 batches, fresh or aged at 45 degrees C/48 h, had their F concentrations and pH analyzed with a specific electrode and pHmeter. To analyze the TF, 0.25 ml of 2M HCL was added to 0.25 ml of the suspension of each dentifrice. This was kept at 45 degrees C/1 h and 0.50 ml of M NaOH and 1.0 ml of TISAB II was added. To assay SF and IF, after centrifugation, the supernatant was used and the same steps described above were followed for SF. The analysis of IF was made by adding 0.25 ml of the supernatant to 1.0 ml of TISAB II, 0.5 ml of M NaOH and 0.25 ml of 2M HCL. Paired t tests were used for statistical analysis. RESULTS: The concentration of TF, SF and IF ranged from 555.7 - 1835.1; 449.3 - 1619.3 and 96.9 - 1534.6 ppm respectively. After aging, a significant reduction in the SF concentration (p = 0.00002) was observed, which did not occur for TF and IF (p > 0.05). Only the brand Colgate Baby presented pH 6.8. The other dentifrices had an alkaline pH, ranging from 7.1 - 9.9. CONCLUSION: Although the term 'soluble' was suppressed by the new regulation, the dentifrices analyzed were in accordance to the Brazilian Ministry of Health Regulations, 1989.