Micromorphological and hardness analyses of human and bovine sclerotic dentin: a comparative study.

The purpose of this study was to test the hypothesis that both human and bovine sclerotic dentin have similar hardness properties, in addition to similar micromorphological characteristics. Sixteen teeth (8 human and 8 bovine) exhibiting exposed dentin in the incisal edge and showing characteristics typical of sclerosis were used. Vickers surface microhardness testing was conducted. Three areas of the dentin surface of each specimen were selected. All teeth were processed for scanning electron microscopy in order to estimate the amount (in percentage) of solid dentin on the sclerotic dentin surface. The data were compared by Student's t test (α = 0.05). The micromorphological and microhardness data were compared by Pearson's linear correlation test (α = 0.05). The mean percentages of solid dentin of human and bovine sclerotic dentin were similar (human 90.71 ± 0.83 and bovine 89.08 ± 0.81, p = 0.18). The mean microhardness value (VHN) of human sclerotic dentin was significantly higher than that of bovine sclerotic dentin (human 45.26 ± 2.92 and bovine 29.93 ± 3.83, p = 0.006). No correlation was found between the microhardness values and the amount of solid dentin in the sclerotic dentin, irrespective of the species considered (human R² = 0.0240, p = 0.714; bovine R² = 0.0017, p = 0.923; and combined R² = 0.038, p = 0.46). We concluded that although both bovine and human sclerotic dentin present a similar amount of solid tissue, human sclerotic dentin presents higher microhardness than bovine sclerotic dentin.

Reattachment of rehydrated dental fragment using two techniques.

UNLABELLED: The reattachment of dental fragments is a conservative treatment and should be considered in the restoration of anterior tooth fractures. This study compared the fracture strength of dehydrated and rehydrated tooth fragments submitted to two different bonding techniques. MATERIALS AND METHODS: Sixty human central and lateral mandibular incisors were divided into six groups and sectioned 3 mm from the incisal edge, using a diamond disk. Two reattachment techniques were applied: (a) bonding, using the Single Bond adhesive system and FiltekZ250 composite resin, followed by placement of a chamfer on the fracture line that was filled with composite resin (Groups 1, 3 and 5); and (b) use of the same bonding technique after dentin removal from the tooth fragment (Groups 2, 4 and 6). The following hydration treatments were applied to the fragments before bonding: (a) 48-h hydration (Groups 1 and 2); (b) 48-h dehydration (Groups 3 and 4); (c) 48-h dehydration followed by rehydration 30 min before bonding (Groups 5 and 6). The reattached teeth were mounted in acrylic resin cylinders and stored in distilled water for 24 h. The specimens were fractured at a speed of 1 mm min(-1) in a universal testing machine. RESULTS: The following mean fracture strengths (kgf) were recorded: (G1) 12.9 +/- 0.6; (G2) 18.8 +/- 4.8; (G3) 7.3 +/- 1.5; (G4) 15.2 +/- 2.4; (G5) 13.4 +/- 2.2; and (G6) 17.1 +/- 3.2. Analyses using two-way anova and the Tukey test (P < 0.01) revealed significant differences between the restorative techniques and the hydration treatments. CONCLUSIONS: The bonding technique that incorporated dentin removal from the fragment before bonding showed greater fracture strength across all groups. Fragment dehydration for 48 h caused a reduction in fracture strength, which was recovered by a 30-min rehydration.

Micro-tensile bond strength to bovine sclerotic dentine: influence of surface treatment.

OBJECTIVE: The objective of this study was to evaluate the influence of the surface treatment and acid conditioning (AC) time of bovine sclerotic dentine on the micro-tensile bond strength (micro-TBS) to an etch and rinse adhesive system. MATERIALS AND METHOD: Thirty-six bovine incisors were divided into six groups (n=6): G1 sound dentine submitted to AC for 15s; G2-G6 sclerotic dentine: G2-AC for 15s; G3-AC for 30s; G4-EDTA and AC for 15s; G5-diamond bur and AC for 15s; G6-diamond paste and AC for 15s. An adhesive system was applied to the treated dentine surfaces followed by a hybrid composite inserted in increments and light cured. After 24h storage in water at 37 degrees C, the specimens were perpendicularly cut with a low-speed diamond saw to obtain beams (0.8 mm x 0.8 mm cross-sectional dimensions) for micro-TBS testing. Data was compared by ANOVA followed by Tukey's test (p

Morphological analysis of human and bovine dentine by scanning electron microscope investigation.

OBJECTIVE: The objective of this study was to compare the superficial morphology of bovine and human sclerotic dentine. DESIGN: For the morphological analysis, bovine (n = 3) and human (n = 3) incisors exhibiting exposed dentine were used. Dentine presented characteristics of sclerosis: brownish, smooth and shiny--the vitreous appearance. The teeth were prepared for assessment on a scanning electron microscope (SEM). Three pre-determined areas of each sample were submitted to SEM. The number of open tubules per area was obtained from the electron micrographs (n = 9 per group) for comparison purposes. RESULTS: The number of open tubules in both species compared were similar (p > 0.05). Human dentine presented 31.89 +/- 23.94 open tubules per area, whereas bovine dentine showed 30.33 +/- 18.14 open tubules per area. CONCLUSION: Based on the results, we concluded that dentine exposed at the incisal surface of human and bovine teeth presented similar clinical and micro-morphological aspects, represented by surfaces with equivalent numbers of open dentinal tubules.

Resistência à fratura de restauraçöes diretas com cobertura de cúspide em pré-molares superiores endodonticamente tratados / Resistance to fracture of direct restorations with cuspal coverage in upper endodontically

Dentes tratados endodonticamente säo considerados mais susceptíveis à fratura em funçäo da perda de estrutura dentária. Este estudo teve como objetivo avaliar o aumento da resistência à fratura de pré-molares superiores que receberam acesso endodôntico e foram restaurados em resina composta com cobertura de cúspide. Foram utilizados 40 pré-molares superiores humanos extraídos, que foram divididos em 4 grupos: I-dentes hígidos; II-dentes com acesso endodôntico, com preparo MOD, restaurados em resina composta, sem cobertura de cúspide; III-dentes com acesso endodôntico, com preparo MOD e reduçäo de cúspide, restaurados em resina composta, com cobertura de cúspide; IV-dentes com acesso endodôntico, com preparo MOD, sem receber restauraçäo. Os corpos-de-prova foram submetidos a teste de compressäo até a fratura e os resultados foram submetidos a análise de variância e comparados através do teste de Tukey, podendo-se concluir que os dentes do grupo III (restaurados com cobertura de cúspide) apresentaram maior resistência à fratura, com valores significativos, em relaçäo aos dentes do grupo II (restaurados sem cobertura de cúspide) a IV (só preparo cavitário sem restauraçäo). A restauraçäo em resina composta direta com cobertura de cúspide pode ser considerada uma alternativa restauradora para dentes pré-molares submetidos à terapia endodôntica