RESUMO
A polyclonal CD3(+), CD8(+) T-cell line, G2, was derived from the peripheral blood of a seropositive, PCR-positive, HTLV-IIB infected Guahibo Indian from Venezuela. The cell line is productively infected with HTLV-IIB. The entire HTLV-II G2 proviral DNA was sequenced via PCR using overlapping HTLV-II primer pairs. Phylogenetic analyses indicate that HTLV-II G2 is the most divergent HTLV-IIB strain identified to date. Characterization of its deduced proteins and its relationship to other members of the PTLV/BLV genus of retroviruses are discussed.
Assuntos
Genoma Viral , Vírus Linfotrópico T Tipo 2 Humano/genética , Indígenas Sul-Americanos , Sequência de Aminoácidos , Células Cultivadas , DNA Viral/análise , Vírus Linfotrópico T Tipo 2 Humano/classificação , Vírus Linfotrópico T Tipo 2 Humano/isolamento & purificação , Humanos , Imunofenotipagem , Dados de Sequência Molecular , Homologia de Sequência de Aminoácidos , VenezuelaRESUMO
In attention to the important HIV-1 seroprevalence observed in Margarita Island, we carried out this study to establish HTLV-I/II seroprevalence into target groups for sexual transmission. Therefore the survey was done with 141 female sex workers and 40 Gay men between 1994 and 1997. We found HTLV-I infection in one man. This is the first known report to describe epidemiological features of HTLV-I/II infection in Margarita Island.