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1.
RSC Adv ; 14(15): 10481-10498, 2024 Mar 26.
Artigo em Inglês | MEDLINE | ID: mdl-38567345

RESUMO

We introduce a liquid chromatography - mass spectrometry with data-independent acquisition (LC-MS/DIA)-based strategy, specifically tailored to achieve comprehensive and reliable glycosylated flavonoid profiling. This approach facilitates in-depth and simultaneous exploration of all detected precursors and fragments during data processing, employing the widely-used open-source MZmine 3 software. It was applied to a dataset of six Ocotea plant species. This framework suggested 49 flavonoids potentially newly described for these plant species, alongside 45 known features within the genus. Flavonols kaempferol and quercetin, both exhibiting O-glycosylation patterns, were particularly prevalent. Gas-phase fragmentation reactions further supported these findings. For the first time, the apigenin flavone backbone was also annotated in most of the examined Ocotea species. Apigenin derivatives were found mainly in the C-glycoside form, with O. porosa displaying the highest flavone : flavonol ratio. The approach also allowed an unprecedented detection of kaempferol and quercetin in O. porosa species, and it has underscored the untapped potential of LC-MS/DIA data for broad and reliable flavonoid profiling. Our study annotated more than 50 flavonoid backbones in each species, surpassing the current literature.

2.
Front Pharmacol ; 15: 1356598, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38666018

RESUMO

Introduction: Asthma is a condition of airflow limitation, common throughout the world, with high mortality rates, especially as it still faces some obstacles in its management. As it constitutes a public health challenge, this study aimed to investigate the effect of copaiba oil (e.g., Copaifera langsdorffii), as a treatment resource, at doses of 50 and 100 mg/kg on certain mediators of acute lung inflammation (IL-33, GATA3, FOXP3, STAT3, and TBET) and early mechanisms of lung remodeling (degradation of elastic fiber tissues, collagen deposition, and goblet cell hyperplasia). Methods: Using an ovalbumin-induced acute allergic asthma model in BALB/c mice, we analyzed the inflammatory mediators through immunohistochemistry and the mechanisms of lung remodeling through histopathology, employing orcein, Masson's trichrome, and periodic acid-Schiff staining. Results: Copaiba oil treatment (CO) reduced IL-33 and increased FOXP3 by stimulating the FOXP3/GATA3 and FOXP3/STAT3 pathways. Additionally, it upregulated TBET, suggesting an additional role in controlling GATA3 activity. In the respiratory epithelium, CO decreased the fragmentation of elastic fibers while increasing the deposition of collagen fibers, favoring epithelial restructuring. Simultaneously, CO reduced goblet cell hyperplasia. Discussion: Although additional research is warranted, the demonstrated anti-inflammatory and re-epithelializing action makes CO a viable option in exploring new treatments for acute allergic asthma.

3.
Results Chem ; 5: 100761, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36619209

RESUMO

Hydroxychloroquine (HCQ) displays attractive anti-inflammatory and antiviral effects. Because of that, such a drug made part of some clinical trials for combating Sars-CoV-2 during the COVID-19 pandemic. The present study aimed to conduct the biotransformation of HCQ by filamentous fungi reported as microbial models of mammalian drug metabolism to evaluate its cytotoxic after metabolization. Cunninghamella echinulata var. elegans ATCC 8688a could efficiently biotransform HCQ into one main metabolite identified as the new 4-(1,2,3,4-tetrahydroquinolin-4-ylamino)pentan-1-ol (HCQ-M). The microbial transformation occurred through N-dealkylation, 7-chloro-elimination, and reduction of the two conjugated double-bond from the quinoline system of HCQ. The cytotoxic profiles of HCQ and its metabolite were evaluated using CCD-1059Sk cells (human fibroblasts) through sulforhodamine B, trypan blue, and Live/Dead assays. Both HCQ and HCQ-M displayed cytotoxic activities in human fibroblasts, but HCQ-M was significantly more toxic than HCQ. The reported findings should be considered for further clinical studies of HCQ and will be important for guidance in achieving new derivatives from it.

4.
J Pain Symptom Manage ; 64(6): e323-e330, 2022 12.
Artigo em Inglês | MEDLINE | ID: mdl-35985550

RESUMO

CONTEXT: The instrument used to assess neonatal pain must be adequate regarding the type of pain, population, country, and language to provide the best evidence-based clinical strategies; however, few neonatal pain instruments have been translated and validated for the Brazilian population. OBJECTIVE: The aim was to perform a cross-cultural adaptation of the COMFORTneo scale into Brazilian Portuguese and to evaluate the content validity of the adapted scale. METHODS: The cross-cultural adaptation process followed six main steps: translation, synthesis of the translations, back-translation, submission to the expert committee, final version pretest, being that 65 individuals participated in this stage, including both healthcare professionals and students, and submission to the committee for process appraisal. Additionally, an equivalence form composed of a four-point Likert scale was sent to each committee participant to calculate the content validity index (CVI). The CVI was obtained as the sum of the items ranked as three or four by the experts divided by the total number of experts. RESULTS: No difficulties were reported in the production of translated versions. The CVI for the final version of the translated instrument was 0.99. The final version was reviewed to correct any possible grammatical errors. The layout was modified as necessary, and instructions on scale scoring were added to facilitate the application, resulting in the COMFORTneo Brazil scale. CONCLUSION: The COMFORTneo scale was properly and cross-culturally adapted into Brazilian Portuguese, reaching semantic, idiomatic, experimental, and conceptual equivalence with the original instrument, and a good CVI.


Assuntos
Comparação Transcultural , Idioma , Recém-Nascido , Humanos , Brasil , Inquéritos e Questionários , Traduções , Dor/diagnóstico , Reprodutibilidade dos Testes
5.
Nursing (Ed. bras., Impr.) ; 24(278): 5912-5921, jul.-2021.
Artigo em Português | LILACS, BDENF - Enfermagem | ID: biblio-1343118

RESUMO

Objetivo: identificar a adesão dos profissionais de um hospital escola, quanto a utilização dos indicadores de segurança do paciente preconizados pelo Ministério da Saúde. Método: estudo descritivo exploratório, retrospectivo, de abordagem quantitativa, foi avaliado a adesão aos indicadores de segurança do paciente no período de outubro de 2019 a março de 2020. A coleta de dados foi no mês de março de 2020, no Núcleo de Segurança do Paciente e Gestão da Qualidade, por meio de dados coletados mensalmente pelo setor. Resultados: A taxa média de adesão à pulseira de identificação foi 81,9%; identificação do paciente, taxa média de 61,7%; média de adesão à escala de Braden de 80,6% e a taxa de adesão a Escala de Morse, de 78,5%. Conclusão: o não cumprimento dos protocolos instituídos pela instituição relativa à segurança do paciente expõe os usuários a eventos adversos preveníeis e compromete a qualidade da assistência em saúde.(AU)


Objective: to identify the adherence of professionals from a teaching hospital, regarding the use of patient safety indicators recommended by the Ministry of Health. Method: exploratory, retrospective descriptive study, with a quantitative approach, adherence to patient safety indicators in the period from October 2019 to March 2020. Data collection took place in March 2020, at the Patient Safety and Quality Management Center, through data collected monthly by the sector. Results: The average rate of adherence to the identification bracelet was 81,9%; patient identification, mean rate of 61,7%; average adherence to the Braden scale of 80,6% and the adherence rate to the Morse scale, 78,5%. Conclusion: failure to comply with the protocols established by the institution regarding patient safety exposes users to preventable adverse events and compromises the quality of health care.(AU)


Objetivo: identificar la adherencia de los profesionales de un hospital universitario, respecto al uso de los indicadores de seguridad del paciente recomendados por el Ministerio de Salud. Método: estudio descriptivo, exploratorio, retrospectivo con abordaje cuantitativo, se evaluó la adherencia a los indicadores de seguridad del paciente en el período de octubre de 2019 a marzo de 2020. La recogida de datos tuvo lugar en marzo de 2020, en el Centro Seguridad del Paciente y Gestión de Calidad, utilizando datos recogidos mensualmente por el sector. Resultados: La tasa promedio de adherencia al brazalete de identificación fue del 81,9%; identificación de pacientes, tasa promedio del 61,7%; adherencia media a la Escala de Braden del 80,6% y la tasa de adherencia a la escala Morse del 78,5%. Conclusión: el incumplimiento de los protocolos establecidos por la institución relativos a la seguridad del paciente expone a los usuarios a eventos adversos prevenibles y compromete la calidad de la atención en salud.(AU)


Assuntos
Humanos , Estudos Retrospectivos , Indicadores de Qualidade em Assistência à Saúde , Segurança do Paciente/normas , Qualidade da Assistência à Saúde
6.
Photochem Photobiol ; 96(1): 14-20, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-31400235

RESUMO

There is some evidence in the literature of the photocyclization reaction of Tagitinin C (1) to Tagitinin F (2). Compound 2 has high pharmacological potential, but it is not easy to obtain, while compound 1 is easily obtained from a widespread plant, Tithonia diversifolia. Among different reaction conditions monitored, one was found that allowed the cyclization of 1 into 2 in <15 min in a photo-dependent reaction. Scaling-up the photocyclization of the pure compound 1 into 2 demonstrated 100% yield, and the isolation of 2 from a UV-irradiated extract was eight-fold higher than the quantity isolated from the non-UV-irradiated extract. We were also able to better understand the process of photoconversion and determine methods to isolate and quantify these compounds, which are known for their important antitumoral activities among other important pharmacological properties.


Assuntos
Processos Fotoquímicos , Extratos Vegetais/química , Sesquiterpenos/isolamento & purificação , Cromatografia Líquida de Alta Pressão , Ciclização , Espectroscopia de Ressonância Magnética , Espectrometria de Massas/métodos , Reprodutibilidade dos Testes , Sesquiterpenos/química , Espectrofotometria Ultravioleta , Raios Ultravioleta
7.
Toxicol In Vitro ; 59: 150-160, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31022444

RESUMO

Cancer is one of the most critical problems of public health in the world and one of the main challenges for medicine. Different biological effects have been reported for sulfonamide-based compounds including antibacterial, antifungal, and antitumor activities. Herein, a series of phenylpropanoid-based sulfonamides (4a, 4a', 4b, 4b', 5a, 5a', 5b and 5b') were synthesized and their cytotoxic activity was evaluated against four cell lines derived from human tumours (A549 - lung, MCF-7 - breast, Hep G2 - hepatocellular carcinoma, and HT-144-melanoma). Cell viability was significantly reduced in the MCF-7 cell line when compounds 4b, 4b' and 5a were used; IC50 values were lower than those found for their precursors (eugenol and dihydroeugenol) and sulfanilamide. We observed that 4b induced cell cycle arrest at G1/S transition. This is probably due to its ability to reduce cyclin D1 and cyclin E expression. Moreover, 4b also induced apoptosis in MCF-7 cells as demonstrated by an increase in the cell population positive for annexin V in treated cultures in comparison to the control group. Taken together, the data showed that 4b is a promising antitumor agent and it should be considered for further in vivo studies.


Assuntos
Antineoplásicos/farmacologia , Ciclina D1/metabolismo , Ciclina E/metabolismo , Proteínas Oncogênicas/metabolismo , Fenilpropionatos/farmacologia , Sulfonamidas/farmacologia , Ciclo Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Regulação para Baixo/efeitos dos fármacos , Humanos
8.
Microbes Infect ; 18(2): 137-47, 2016 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-26597327

RESUMO

Paracoccidioidomycosis is caused by fungi of the Paracoccidioides genus and constitutes the most prevalent deep mycosis in Latin America. Toll-like receptors promote immune response against infectious agents. Recently, it was reported that TLR9 is crucial for mice survival during the first 48 h of P. brasiliensis infection. In this study, we used CPG oligodeoxynucleotide motif as an adjuvant with and without rPb27 to immunize mice against Paracoccidioidomycosis. CPG adjuvant induced differential recruitment of lymphocytes in the inflammatory process and a lower recruitment of neutrophils. In addition, CPG induced the production of pro-inflammatory cytokines such as IL-1ß, TNF-α, IL-6 and IL-12; increased phagocytic ability and microbicidal activity by macrophages; and induced differential production of lgG2a and lgG2b, subtypes of Ig. Knockout mice for TLR9 and IL-12 showed higher fungal loads and rates of mortality compared to control mice after 30 days of infection. The association between CPG and rPb27 induced a high level of protection against Paracoccidioidomycosis after the first 30 days of infection but not at 60 days. Our findings demonstrate that TLR 9 plays a role in the protection induced by immunization with rPb27 and confirms the importance of TLR9 in the initial protection against Paracoccidioidomycosis.


Assuntos
Vacinas Bacterianas/imunologia , Proteínas Fúngicas/imunologia , Paracoccidioides/imunologia , Paracoccidioidomicose/prevenção & controle , Receptor Toll-Like 9/metabolismo , Adjuvantes Imunológicos/administração & dosagem , Animais , Vacinas Bacterianas/administração & dosagem , Vacinas Bacterianas/genética , Contagem de Colônia Microbiana , Proteínas Fúngicas/genética , América Latina , Camundongos Endogâmicos BALB C , Camundongos Knockout , Oligodesoxirribonucleotídeos/administração & dosagem , Paracoccidioidomicose/imunologia , Proteínas Recombinantes/genética , Proteínas Recombinantes/imunologia , Análise de Sobrevida , Vacinas Sintéticas/administração & dosagem , Vacinas Sintéticas/genética , Vacinas Sintéticas/imunologia
9.
Biomed Res Int ; 2015: 652474, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26146626

RESUMO

Arterial bypass graft implantation remains the primary therapy for patients with advanced cardiovascular disease, but most lack adequate saphenous vein or other conduits for bypass procedures and would benefit from a bioartificial conduit. This study aimed to produce human endothelial cells (hECs) in large scale, free from xenogeneic antigens, to develop a small diameter, compatible vessel for potential use as a vascular graft. Human adipose-derived stromal cells (hASCs) were isolated, cultured, and differentiated in the presence of human serum and used for the reendothelization of a decellularized rat aorta. hASC derived ECs (hASC-ECs) expressed VEGFR2, vWf and CD31 endothelial cell markers, the latter in higher levels than hASCs and HUVECs, and were shown to be functional. Decellularization protocol yielded aortas devoid of cell nuclei, with preserved structure, including a preserved basement membrane. When seeded with hASC-ECs, the decellularized aorta was completely reendothelized, and the hASC-ECs maintained their phenotype in this new condition. hASCs can be differentiated into functional hECs without the use of animal supplements and are capable of reendothelizing a decellularized rat aorta while maintaining their phenotype. The preservation of the basement membrane following decellularization supported the complete reendothelization of the scaffold with no cell migration towards other layers. This approach is potentially useful for rapid obtention of compatible, xenogeneic-free conduit.


Assuntos
Antígenos Heterófilos/biossíntese , Aorta/crescimento & desenvolvimento , Prótese Vascular , Células Endoteliais/metabolismo , Animais , Antígenos Heterófilos/imunologia , Aorta/metabolismo , Diferenciação Celular/genética , Movimento Celular/genética , Células Endoteliais/imunologia , Humanos , Ratos , Engenharia Tecidual , Transplantes
10.
Biomed Res Int ; 2015: 143504, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25688350

RESUMO

Human adipose-derived stem cells (hASCs) are an attractive cell source for therapeutic applicability in diverse fields for the repair and regeneration of damaged or malfunctioning tissues and organs. There is a growing number of cell therapies using stem cells due to their characteristics of modulation of immune system and reduction of acute rejection. So a challenge in stem cells therapy is the delivery of cells to the organ of interest, a specific site. The aim of this paper was to investigate the effects of a supramolecular assembly composed of single-walled carbon nanotubes (SWCNT), molecular magnets (lawsone-Co-phenanthroline), and a synthetic peptide (FWYANHYWFHNAFWYANHYWFHNA) in the hASCs cultures. The hASCs were isolated, characterized, expanded, and cultured with the SWCNT supramolecular assembly (SWCNT-MA). The assembly developed did not impair the cell characteristics, viability, or proliferation. During growth, the cells were strongly attached to the assembly and they could be dragged by an applied magnetic field of less than 0.3 T. These assemblies were narrower than their related allotropic forms, that is, multiwalled carbon nanotubes, and they could therefore be used to guide cells through thin blood capillaries within the human body. This strategy seems to be useful as noninvasive and nontoxic stem cells delivery/guidance and tracking during cell therapy.


Assuntos
Imãs/química , Células-Tronco Mesenquimais/citologia , Células-Tronco Mesenquimais/fisiologia , Nanotubos de Carbono/química , Peptídeos/química , Tecido Adiposo/citologia , Separação Celular/métodos , Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Humanos , Transplante de Células-Tronco Mesenquimais , Nanotubos de Carbono/toxicidade , Naftoquinonas/química , Fenantrolinas/química
11.
Stem Cell Rev Rep ; 10(5): 697-711, 2014 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-24913281

RESUMO

Human orbital fat tissues are a potential source to isolate stem cells for the development of regenerative medicine therapies. For future safe clinical application of these cells, it is critical to establish animal component-free culture conditions as well as to clearly define the stem cell population characteristics differentiating them from other cell types, such as fibroblasts. Therefore, the present study aimed to compare phenotypic and functional characteristics of orbital fat-derived stem cells (OFSCs) and fibroblasts resident in the eyelid skin in donor-matched samples grown in culture medium supplemented with pooled allogeneic human serum (HS) replacing fetal bovine serum (FBS). We first investigated the proliferative effects of OFSCs on HS, and then we compared the alkaline phosphatase (AP) expression and activity, immunophenotypic profile, and in vitro multilineage differentiation potential of OFSCs side-by-side with fibroblasts. The results showed that HS enhanced OFSCs proliferation without compromising their immunophenotype, AP activity, and osteogenic, adipogenic, and chondrogenic differentiation capacities. In contrast to OFSCs, the fibroblasts did not exhibit AP expression and activity and did not have multilineage differentiation potential. The results enabled us to successfully distinguish OFSCs from fibroblasts populations, suggesting that AP expression/activity and multilineage differentiation assays can be used reliably to discriminate mesenchymal stem cells from fibroblasts. Our findings also support the feasibility of pooled allogeneic HS as a safer and more effective alternative to FBS for clinical applications.


Assuntos
Tecido Adiposo/citologia , Fosfatase Alcalina/metabolismo , Diferenciação Celular/efeitos dos fármacos , Meios de Cultura Livres de Soro/farmacologia , Olho , Fibroblastos/citologia , Órbita , Células-Tronco/citologia , Fosfatase Alcalina/genética , Linhagem da Célula/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Células Cultivadas , Fibroblastos/efeitos dos fármacos , Fibroblastos/enzimologia , Humanos , Células-Tronco/efeitos dos fármacos , Células-Tronco/enzimologia , Relação Estrutura-Atividade
12.
J Mater Sci Mater Med ; 25(4): 1137-48, 2014 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-24378848

RESUMO

Mineralized poly(ε-caprolactone)/gelatin core-shell nanofibers were prepared via co-axial electrospinning and subsequent incubation in biomimetic simulated body fluid containing ten times the calcium and phosphate ion concentrations found in human blood plasma. The deposition of calcium phosphate on the nanofiber surfaces was investigated through scanning electronic microscopy and X-ray diffraction. Energy dispersive spectroscopy results indicated that calcium-deficient hydroxyapatite had grown on the fibers. Fourier transform infrared spectroscopy analysis suggested the presence of hydroxyl-carbonate-apatite. The results of a viability assay (MTT) and alkaline phosphatase activity analysis suggested that these mineralized matrices promote osteogenic differentiation of human adipose-derived stem cells (hASCs) when cultured in an osteogenic medium and have the potential to be used as a scaffold in bone tissue engineering. hASCs cultured in the presence of nanofibers in endothelial differentiation medium showed lower rates of proliferation than cells cultured without the nanofibers. However, endothelial cell markers were detected in cells cultured in the presence of nanofibers in endothelial differentiation medium.


Assuntos
Tecido Adiposo/citologia , Células-Tronco Adultas/citologia , Materiais Biocompatíveis/química , Nanofibras/química , Células-Tronco Adultas/enzimologia , Fosfatase Alcalina/metabolismo , Diferenciação Celular , Proliferação de Células , Sobrevivência Celular , Células Endoteliais/citologia , Gelatina/química , Humanos , Teste de Materiais , Microscopia Eletrônica de Varredura , Minerais/química , Nanofibras/ultraestrutura , Osteogênese , Poliésteres/química
13.
Tissue Eng Part A ; 19(1-2): 277-89, 2013 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-22920790

RESUMO

Human adipose-derived stem cells (hASCs) are currently a point of focus for bone tissue engineering applications. However, the ex vivo expansion of stem cells before clinical application remains a challenge. Fetal bovine serum (FBS) is largely used as a medium supplement and exposes the recipient to infections and immunological reactions. In this study, we evaluated the osteogenic differentiation process of hASCs in poly-3-hydroxybutyrate-co-3-hydroxyvalerate (PHB-HV) scaffolds with the osteogenic medium supplemented with pooled allogeneic human serum (aHS). The hASCs grown in the presence of FBS or aHS did not show remarkable differences in morphology or immunophenotype. The PHB-HV scaffolds, which were developed by the freeze-drying technique, showed an adequate porous structure and mechanical performance as observed by micro-computed tomography, scanning electron microscopy (SEM), and compression test. The three-dimensional structure was suitable for allowing cell colonization, which was revealed by SEM micrographs. Moreover, these scaffolds were not toxic to cells as shown by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The differentiation capacity of hASCs seeded on scaffolds was confirmed by the reduction of the proliferation, the alkaline phosphatase (AP) activity, expression of osteogenic gene markers (AP, collagen type I, Runx2, and osteocalcin), and the expression of bone markers, such as osteopontin, osteocalcin, and collagen type I. The osteogenic capacity of hASCs seeded on PHB-HV scaffolds indicates that this scaffold is adequate for cell growth and differentiation and that aHS is a promising supplement for the in vitro expansion of hASCs. In conclusion, this strategy seems to be useful and safe for application in bone tissue engineering.


Assuntos
Adipócitos/citologia , Adipócitos/metabolismo , Osteoblastos/citologia , Osteogênese/fisiologia , Poliésteres/química , Soro/metabolismo , Alicerces Teciduais , Diferenciação Celular , Células Cultivadas , Desenho de Equipamento , Análise de Falha de Equipamento , Humanos , Osteoblastos/metabolismo , Proibitinas
14.
Rev Bras Enferm ; 65(1): 172-8, 2012.
Artigo em Português | MEDLINE | ID: mdl-22751726

RESUMO

This article describes the general competence of health care professionals and points possible articulations with the practicum. The goal is to draw the attention of nurses and future professionals, about the importance of experiencing the practicum, during training for the acquisition and improvement of general competence of health care professionals, in addition to the specific skills and competencies of nursing. In the health services, both in public and in private, there is the need to count on a competent nurse professional in your professional practice. In this way, as is noted in the National curriculum guidelines for undergraduate courses in nursing, the practicum is considered the best place for the graduating acquire a profile that meets the profile of a competent professional with knowledge and skills appropriate to the exercise of the profession.


Assuntos
Competência Clínica , Educação em Enfermagem/normas , Enfermagem/normas
15.
Phytomedicine ; 15(10): 815-25, 2008 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-18434121

RESUMO

The aim of the present study is to investigate the antiulcerogenic effects of the essential oil (EO) of Croton cajucara Benth in rats fed with a normal protein (NP) and low-protein diet (MN). NP and MN rats were treated with the essential oil for 15 days after chronic ulceration was induced. The EO accelerated healing of acetic acid-induced gastric lesions in NP and MN rats (p<0.05). In a similar experiment on chronic ulceration, Epidermal Growth Factor (EGF) mRNA expression increased in NP rats but not in MN rats. In assays of acute antiulcerogenic activity, C. cajucara increased somatostatin plasma levels and decreased gastrin plasma levels in both animal groups. The EO significantly prevented ethanol-induced gastric ulcers in NP and MN rats (p<0.001). Histological examination showed initial regeneration, formation of inflammatory infiltrate and angiogenesis in the epithelium surface of acetic acid-induced ulcers in NP and MN rats. C. cajucara prevented gastric lesions in both animal groups when ethanol methodology was used. We concluded that the EO showed an antiulcerogenic activity mediated by increased somatostatin secretion and EGF mRNA expression.


Assuntos
Antiulcerosos/farmacologia , Croton/química , Desnutrição/complicações , Ácido Acético/toxicidade , Animais , Sequência de Bases , Primers do DNA , Proteínas Alimentares/administração & dosagem , Fator de Crescimento Epidérmico/genética , Feminino , Gastrinas/sangue , Úlcera Péptica/induzido quimicamente , Úlcera Péptica/complicações , Úlcera Péptica/prevenção & controle , RNA Mensageiro/genética , Ratos , Ratos Wistar , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Somatostatina/sangue
16.
J Ethnopharmacol ; 95(2-3): 345-51, 2004 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-15507358

RESUMO

The alkaloid extract of Senecio brasiliensis inflorescences contain a mixture of the pyrrolizidine alkaloids (PA) senecionine, integerrimine, retrorsine, usaramine and seneciphylline. We evaluated this PA mixture on preventive antiulcerogenic effects on standard rodent models of induced gastric and duodenal ulcers. In the HCl/ethanol, indomethacin-bethanechol and hypothermic-restraint-induced gastric ulcer, the lesion was significantly inhibited by PA (p.o.) (p < 0.001). In the pylorus-ligature, PA (i.d.), significantly increased the gastric juice content and the pH values and decreased the acid output. In the cysteamine induced duodenal ulcers, PA (p.o.) showed significant inhibition (p < 0.001) of the duodenal lesions when compared to the respective control. The levels of the somatostatin hormone in the blood samples of animals pre-treated with the PA (12.5 mg/kg) and the free mucus and prostaglandin synthesis also increased (p < 0.001) after administration of PA extract (p.o.). The results suggested that the PA extract from Senecio brasiliensis inflorescences presents a significant anti-ulcer effect in the selected ulcer models. The mechanism involved with the action of the PA extract is the cytoprotection. Additional studies are in progress to determine other possible mechanisms involved with effect of the PA as anti-ulcer agents.


Assuntos
Úlcera Duodenal/prevenção & controle , Alcaloides de Pirrolizidina/uso terapêutico , Senécio , Úlcera Gástrica/prevenção & controle , Animais , Anti-Inflamatórios não Esteroides/toxicidade , Relação Dose-Resposta a Droga , Úlcera Duodenal/induzido quimicamente , Masculino , Camundongos , Alcaloides de Pirrolizidina/química , Alcaloides de Pirrolizidina/isolamento & purificação , Ratos , Úlcera Gástrica/induzido quimicamente
17.
Can J Physiol Pharmacol ; 82(5): 319-25, 2004 May.
Artigo em Inglês | MEDLINE | ID: mdl-15213731

RESUMO

We investigated the antiulcerogenic activity of pyrrolizidine alkaloids (PAs) integerrimine, retrorsine, senecionine, usaramine and seneciplhylline, an alkaloidal extract obtained from Senecio brasiliensis. The PA extract demonstrated significantly activity in both, acute and chronic gastric ulcers on rats. The effects of PA extract were dose dependent. The mechanisms implicated on this activity were evaluated by determination of gastrin plasma levels in rats subjected to the acute treatment with PA extract and by expression of mRNA of Epidermal Growth Factor (EGF) after chronic treatment with this extract. The results showed that the PA extract increased both the levels of gastrin and the expression of EGF on these animals. Moreover, the histological examinations showed a reduction of exfoliation of superficial cells, hemorrhages and blood cell infiltration. We concluded that the PAs showed an important and qualitative antiulcerogenic activity mediated by increase in gastrin secretion and mRNA expression of EGF.


Assuntos
Fator de Crescimento Epidérmico/biossíntese , Gastrinas/biossíntese , Alcaloides de Pirrolizidina/uso terapêutico , Senécio , Úlcera Gástrica/tratamento farmacológico , Animais , Relação Dose-Resposta a Droga , Masculino , Camundongos , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Alcaloides de Pirrolizidina/química , Alcaloides de Pirrolizidina/isolamento & purificação , Ratos , Úlcera Gástrica/metabolismo , Úlcera Gástrica/patologia
18.
Biol Pharm Bull ; 25(4): 452-6, 2002 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-11995923

RESUMO

The nor-clerodane diterpene trans-crotonin isolated from the bark of Croton cajucara BENTH. was investigated for its ability to prevent the formation of gastric-mucosa ulceration in different experimental models in mice. The results obtained from crotonin were compared with those obtained with another diterpene, DHC (trans-dehydrocrotonin) in the same models. When previously administered (p.o.) at the dose of 100 mg/kg, crotonin, as well as DHC, significantly reduced (p<0.05) gastric injury induced by stress (72, 67%), indomethacin/bethanechol (78, 29%) and pylorus ligature (35, 30%). In the HCl/ethanol-induced gastric ulcer model, at oral doses of 100 and 250 mg/kg, crotonin significantly prevented (p<0.05) the formation of gastric lesions by 51 and 56%, respectively, when compared to the control group. Gastric injury was also of significantly less magnitude in the DHC treatment group (p<0.05). In the pylorus-ligature model, crotonin (p.o.), like cimetidine, increased the volume of gastric juice when compared to the control group (p<0.05). No significant modifications where found in gastric parameters such as pH or total acid content after oral crotonin treatment. However, systemic alterations were observed when crotonin (100 mg/kg) was previously administered intraduodenally to mice. We observed significant changes (p<0.001) in gastric-juice parameters such as an increase in volume and a decrease in gastric acidity. Those pre-treated with crotonin as well as with DHC did not increase free mucus production (p>0.05). The results suggest that crotonin presents a significant anti-ulcer effect when assessed in these ulcer-induced models. As with DHC, the antiulcerogenic effects of crotonin are probably related to anti-secretory or/and gastroprotective properties of this substance. In light of results obtained with DHC and natural trans-crotonin in the present study, we concluded that the A-ring of both diterpenes is not directly involved in the antiulcerogenic activity.


Assuntos
Antiulcerosos/uso terapêutico , Croton , Diterpenos Clerodânicos , Diterpenos/uso terapêutico , Casca de Planta , Animais , Antiulcerosos/química , Antiulcerosos/isolamento & purificação , Croton/química , Diterpenos/química , Diterpenos/isolamento & purificação , Masculino , Camundongos , Casca de Planta/química , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Extratos Vegetais/uso terapêutico , Úlcera Gástrica/tratamento farmacológico
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