RESUMO
1. "In vitro" incubation of red blood cells with phosmetoxon induced crenated and invaginated forms. 2. [32P] phosphate incorporation was greater in membranes from erythrocytes exposed to 300 nM phosmetoxon for 10 min than in control cells. 3. The highest incorporation was for phosphatidylinositol (PI), followed by phosphatidylinositol phosphate (PIP) and phosphatidylinositolbiphosphate (PIP2). 4. An activation of phosphatidylinositol (PI) kinase was detected with 150 and 300 nM of the pesticide, while there was no change in poliphosphoinositides (PPI) phosphodiesterase activity. 5. Results suggest an association between changes in PI kinase activity, the phosphorylation cycle of phosphatidylinositols and alterations in erythrocyte morphology induced by phosmetoxon.
Assuntos
Eritrócitos/efeitos dos fármacos , Fosmet/farmacologia , Fosfatidilinositóis/metabolismo , 1-Fosfatidilinositol 4-Quinase , Tamanho Celular/efeitos dos fármacos , Eritrócitos/citologia , Eritrócitos/metabolismo , Humanos , Técnicas In Vitro , Lipídeos/análise , Microscopia Eletrônica de Varredura , Fosmet/metabolismo , Fosfoinositídeo Fosfolipase C , Diester Fosfórico Hidrolases/análise , Fosforilação/efeitos dos fármacos , Fosfotransferases (Aceptor do Grupo Álcool)/análiseRESUMO
1. The effect of Dieldrin on phospholipid content and composition was compared in adult frog liver and in early embryos. 2. Male frogs are more resistant to acute Dieldrin treatment than females. Total phospholipids content increases in the liver of male animals while in females decreases. 3. Early embryo development was not affected morphologically by the addition of 0.02-10.0 mg of Dieldrin/1; however phospholipid content and composition was altered. 4. Adaptive changes in the embryo due to chronically administered Dieldrin is suggested.
