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1.
Parasitol Res ; 107(1): 239-42, 2010 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-20454805

RESUMO

Chagas disease, caused by Trypanosoma cruzi, is an important public health problem in Latin America. Disturbances in gastrointestinal motility are observed in 15-20% of patients at the chronic phase. We previously observed a decrease in intestinal motility in mice infected with Y strain from T. cruzi. Thus, we decided to test if infection with other T. cruzi strains also caused the intestinal disturbance. Male adult Swiss mice were infected intraperitoneally with CL-Brener clone (CL-B), Brazil strain (Br), or Dm28 clone (Dm) of T. cruzi. All infected mice presented a low cumulative mortality (CL-B, 17%; Br, 8%; Dm, 25%) at 35 days post infection (dpi) and their typical parasitemia curves. Br and Dm groups exhibited a maximal reduction of intestinal motility at 35 dpi (176.8 +/- 51.3 and 198.3 +/- 52.6 min, respectively), when compared with non-infected mice (90.2 +/- 19.5 min). However, CL mice presented the peak of delayed intestinal transit at 12 dpi (191.0 +/- 33.3 min), when compared with non-infected mice (105.6 +/- 26.4 min), very close to the 15 dpi for the intense alteration (310.2 +/- 67.4 min) observed with the Y strain. We clearly demonstrate a reduction in intestinal motility in mice infected with different groups of T. cruzi during the acute phase of the infection. Since Br, Dm, and CL strains presented low mortality rates in adult Swiss mice, a prospective study concerning the chronic intestinal alteration is encouraged, particularly for studies of alternative therapies.


Assuntos
Doença de Chagas/patologia , Motilidade Gastrointestinal , Intestinos/fisiologia , Trypanosoma cruzi/patogenicidade , Animais , Masculino , Camundongos , Análise de Sobrevida
2.
Parasitol Res ; 106(6): 1293-8, 2010 May.
Artigo em Inglês | MEDLINE | ID: mdl-20195635

RESUMO

Infection with Trypanosoma cruzi causes megasyndromes of the gastrointestinal (GI) tract in humans and animals. In the present study, we employed magnetic resonance imaging to non-invasively monitor the effect of selenium supplementation on alterations in the GI tract of T. cruzi-infected mice. CD1 mice infected with T. cruzi (Brazil strain) exhibited dilatation of the intestines similar to that we recently reported in infected C57Bl/6 mice. The average intestine lumen diameter increased by 65% and the increase was reduced to 29% in mice supplemented with 2 ppm selenium in the drinking water. When supplemented with 3 ppm selenium in chow the lumen diameter was also significantly reduced although the difference between the infected and infected supplemented mice was smaller. Intestinal motility in infected mice fed with selenium-enriched chow was increased compared with infected mice fed with normal unsupplemented chow and was not significantly different from intestinal motility in uninfected mice. We suggest that Se may be used to modulate the inflammatory, immunological, and/or antioxidant responses involved in intestinal disturbances caused by T. cruzi infection.


Assuntos
Antiprotozoários/uso terapêutico , Doença de Chagas/tratamento farmacológico , Motilidade Gastrointestinal/efeitos dos fármacos , Selênio/uso terapêutico , Trypanosoma cruzi/efeitos dos fármacos , Animais , Doença de Chagas/patologia , Trato Gastrointestinal/patologia , Imageamento por Ressonância Magnética , Masculino , Camundongos , Radiografia Abdominal
3.
Parasitol Res ; 102(4): 747-50, 2008 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-18163190

RESUMO

Chagas disease, caused by the protozoan Trypanosoma cruzi, remains a serious public health problem in Latin America. In relation to digestive problems, 4.5% of patients show mega syndromes (megacolon) in the chronic phase. In this article, we evaluated intestinal motility at the acute phase of T. cruzi infection through charcoal ingestion in adult mice. After infection, Swiss mice were administered an aqueous suspension of charcoal in water by gavage. Decrease in intestinal motility was determined by increased time of appearance of charcoal in the feces. The uninfected group showed a mean time of charcoal elimination of 109.0 +/- 14.6 min throughout the assay. On the other hand, infected mice presented a significant increase in charcoal defecation time during infection. At 15 days postinfection, infected mice showed a significant increase in charcoal defecation time, 310.2 +/- 67.4 min when compared to the uninfected group, which presented 97.8 +/- 31.8 min, indicating that the T. cruzi infection interferes with intestinal motility. Our results demonstrate that the use of charcoal is an ethical and efficient procedure to evaluate the intestinal motility in the murine model of T. cruzi infection.


Assuntos
Doença de Chagas/fisiopatologia , Carvão Vegetal/metabolismo , Modelos Animais de Doenças , Motilidade Gastrointestinal , Trypanosoma cruzi/patogenicidade , Doença Aguda , Adulto , Animais , Doença de Chagas/parasitologia , Fezes/química , Humanos , Masculino , Camundongos
4.
Magn Reson Imaging ; 23(1): 61-8, 2005 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-15733789

RESUMO

Caveolin-1 and caveolin-3 are expressed in the mammalian heart. Mice deficient in caveolin 1 or 3 exhibit cardiac abnormalities including left ventricular hypertrophy and reduced fractional shortening. Cardiac imaging technologies such as transthoracic echocardiography and cardiac-gated magnetic resonance imaging (MRI) are effective tools for the study of left ventricular morphology and function in mice; however, there has not been widespread use of these technologies in studies of right ventricular morphology. In particular, right ventricular wall thickness has been difficult to assess using cardiac imaging technologies. We report here the use of centerline analysis of cardiac-gated MR images to more accurately determine right ventricular wall thickness in the mouse heart. Right ventricular wall thickness was evaluated in Cav-1 null, Cav-3 null and Cav-1/3 null mice, as well as wild-type control mice. Using this technique, we find that caveolin null mice exhibit significant thickening of the right ventricular wall as compared with age-matched wild-type controls. Interestingly, right ventricular wall thickening is greatest in the Cav-1/3 null mice. Furthermore, significant right ventricular wall thickening is also seen in the Cav-1 null mice. Histological analyses revealed right ventricular hypertrophy consistent with the imaging results. These studies demonstrate the utility of MRI in determining right ventricular wall thickness and underscore the severity of the right ventricular hypertrophy in caveolin null mice.


Assuntos
Cardiomiopatia Hipertrófica/diagnóstico , Cardiomiopatia Hipertrófica/fisiopatologia , Caveolinas/genética , Ventrículos do Coração/fisiopatologia , Imageamento por Ressonância Magnética/métodos , Animais , Caveolina 1 , Caveolina 3 , Camundongos
5.
Clin Chem Lab Med ; 41(8): 1020-5, 2003 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-12964807

RESUMO

Micronutrient deficiencies and infectious disease often coexist and show complex interactions leading to mutually reinforced detrimental clinical effects. Such a combination is predominantly observed in underprivileged people of developing countries, particularly in rural regions. Several micronutrients such as trace elements (zinc, iron, selenium) modulate immune function and influence the susceptibility of the host to infection. Nevertheless, the effect of individual micronutrients on components of innate immunity is difficult to design and interpret. Micronutrient deficiency, in general, has a widespread effect on nearly all components of the innate immune response. Chagas' disease is a pertinent model to study interaction of nutrition, immunity and infection, as it implies many components of innate immunity. An important question is whether alterations on micronutrient intake modify the course of infection. Some interactions of trace elements with innate immunity and acute inflammatory response are reviewed in this article with a special focus on selenium deficiency and Trypanosoma cruzi infection.


Assuntos
Imunidade Inata/fisiologia , Parasitos/imunologia , Oligoelementos/fisiologia , Reação de Fase Aguda/imunologia , Animais , Humanos , Deficiências de Ferro , Modelos Biológicos , Nematoides/imunologia , Selênio/deficiência , Trypanosoma cruzi/imunologia , Zinco/deficiência
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