Phytochemical Characterization of Cannabis sativa L. Roots from Northeastern Brazil.

This article describes the phytochemical study of Cannabis sativa roots from northeastern Brazil. The dried plant material was pulverized and subjected to exhaustive maceration with ethanol at room temperature, obtaining the crude ethanolic extract (Cs-EEBR). The volatile compounds were analyzed by gas chromatography coupled with mass spectrometry (GC/MS), which allowed to identify 22 compounds by comparing the linear retention index (LRI), the similarity index (SI) and the fragmentation pattern of the constituents with the literature. By this technique the major compounds identified were: friedelan-3-one and ß-sitosterol. In addition, two fractions were obtained from Cs-EEBR by classical column chromatography and preparative thin layer chromatography. These fractions were analyzed by NMR and IR and together with the mass spectrometry data allowed to identify the compounds: epifriedelanol, friedelan-3-one, ß-sitosterol and stigmasterol. The study contributed to the phytochemical knowledge of Cannabis sativa, specifically the roots, as there are few reports on the chemical constituents of this part of the plant.

Total content of kaurene diterpenes in Annona vepretorum stems via 1 H qNMR: A method for speeding the identification of bioactive extracts.

INTRODUCTION: Kaurene diterpenes (KDs) constitute a chemical class often found in the genus Annona with interesting biological activities. To date, chromatographic tools have been mostly used to determine KDs. Quantitative nuclear magnetic resonance (qNMR) has distinguished itself in quantitative estimation of natural products and is an interesting choice to assess total KD contents. OBJECTIVE: To establish a 1 H qNMR method for determining the total KD contents in extracts and fractions obtained from Annona vepretorum stems. METHODOLOGY: Stems were extracted with hexane and methanol, resulting in the hexane extract (HEX-E) and the methanol extract (MeOH-E). The former was partitioned with the acid-base method to obtain the total alkaloid fraction (TA-F) and the neutral dichloromethane fraction (NDM-F). 1 H qNMR measurements were performed on 400 MHz with samples solubilized in deuterated dimethyl sulfoxide. Quantification was carried out using the signals at 4.71 and 4.78 ppm related to hydrogens of the exocyclic double bond of the basic skeleton of KDs and gallic acid as the standard reference. The selectivity, intra- and inter-day precision, reproducibility, limit of detection, limit of quantification, and robustness of the methodology were evaluated. RESULTS: Using the newly developed method, the total KD contents (in µg/mg) were 653.80 ± 12.15 (HEX-E), 458.90 ± 25.94 (NDM-F), 375.60 ± 27.52 (TA-F), and 315.10 ± 19.20 (MeOH-E). For determining the most promising bioactive sample, the KD contents and the sample discriminations obtained by principal component analysis were correlated to the antibacterial activity. Such approach pointed out HEX-E as a potential source of KDs. CONCLUSION: The developed method offers a fast and simple way of determining total KD contents.