RESUMO
The low level laser therapy (LLLT) has been used as an option to accelerate the regeneration of bone tissue. In this study, both femurs of male Wistar rats (30 animals) were injured with a drill and the effect of LLLT using a laser diode (100 mW at 660 nm) in the bone matrix on the left paw measured. LLLT effect on the healing bone tissue matrix was evaluated by a combination of immunohistochemical histomorphometry, confocal immunofluorescence microscopy and isolation and characterization of glycosaminoglycans. Histomorphometric analysis showed that LLLT increased bone matrix and showing more organized. Alcian Blue and PAS staining seems to suggest differential glycosaminoglycans and glycoproteins. The data showed increased expression of chondroitin sulfate and hyaluronic acid, after reduction as the LLLT and mature bone, resembling the expression of osteonectin and biglycan. The difference in expression of siblings (DMP-1, OPN and BSP) is in accordance with the repair accelerated bone formation after the application of LLLT as compared with control. The expression of osteonectin and osteocalcin supports their role in bone mineralization protein, indicating that LLLT accelerates this process. The overall data show that LLLT bone changes dynamic array, shortening the time period involved in the bone repair.
Assuntos
Matriz Óssea/efeitos da radiação , Regeneração Óssea/efeitos da radiação , Fêmur/efeitos da radiação , Terapia com Luz de Baixa Intensidade , Azul Alciano , Animais , Matriz Óssea/lesões , Sulfatos de Condroitina/biossíntese , Proteínas da Matriz Extracelular/genética , Proteínas da Matriz Extracelular/metabolismo , Fêmur/lesões , Expressão Gênica/efeitos da radiação , Ácido Hialurônico/biossíntese , Imuno-Histoquímica , Sialoproteína de Ligação à Integrina/genética , Sialoproteína de Ligação à Integrina/metabolismo , Lasers , Masculino , Microscopia de Fluorescência , Osteocalcina/genética , Osteocalcina/metabolismo , Osteonectina/genética , Osteonectina/metabolismo , Reação do Ácido Periódico de Schiff , Fosfoproteínas/genética , Fosfoproteínas/metabolismo , Ratos , Ratos WistarRESUMO
Drug-induced gingival enlargement has been reported in patients treated with various types of anticonvulsant drugs, and is generally associated with the presence of plaque, gingival inflammation, and a genetic predisposition. Effective treatment includes daily oral hygiene and periodic professional prophylaxis. However, in some patients, surgical removal of the gingival tissue overgrowth becomes necessary. The patient in this case report was mentally impaired and had severe drug-induced gingival enlargement. This report describes the initial protocol, the gingivectomy, and a 2-year follow-up. A diode laser was used as an effective and safe method to remove the patient's overgrown gingival tissue.
Assuntos
Biofilmes , Crescimento Excessivo da Gengiva/induzido quimicamente , Gengivectomia/métodos , Terapia a Laser , Lasers Semicondutores/uso terapêutico , Anticonvulsivantes/efeitos adversos , Anticonvulsivantes/uso terapêutico , Cálculos Dentários/terapia , Assistência Odontológica para a Pessoa com Deficiência , Placa Dentária/terapia , Epilepsia/tratamento farmacológico , Fibromatose Gengival/induzido quimicamente , Fibromatose Gengival/cirurgia , Seguimentos , Crescimento Excessivo da Gengiva/cirurgia , Humanos , Masculino , Higiene Bucal , Pessoas com Deficiência Mental , Adulto JovemRESUMO
OBJECTIVE: To review the literature regarding the use of surgical lasers in hemangioma treatment and to report a clinical case involving diode laser. BACKGROUND: Hemangiomas are benign vascular proliferations consisting of numerous capillary structures and are especially common on the tongue, lips, jugal mucous membrane, and gums. A number of treatment modalities have been proposed for hemangioma treatment. METHODS: The patient was referred for evaluation complaining of chewing difficulty because of swelling in the right area of the upper lip, with no previous trauma. The lesion was located in the internal right jugal mucous membrane, measured 4 x 4 cm(2), presented a bluish coloration, and was diagnosed as a hemangioma. The treatment selected was surgical removal using gallium arsenide (GaAs) diode laser at 980 nm wavelength, with 4.0 W of power. RESULTS: During surgery, complications including hemorrhage were not observed, and no scarring developed after surgery. CONCLUSIONS: The use of GaAs high-potency diode laser in the treatment of hemangioma reduced bleeding during surgery, with a consequent reduction in operating time, and promoted rapid postoperative hemostasis. It is safe for use on large lesions and easy to manage, and postoperative problems, including potential scarring, and discomfort are minimal.
Assuntos
Hemangioma/cirurgia , Terapia a Laser/métodos , Lasers Semicondutores , Neoplasias Bucais/cirurgia , Feminino , Humanos , Terapia a Laser/instrumentação , Adulto JovemRESUMO
OBJECTIVE: Report a clinical case of surgical lip lesion removal owing to self-injury in a 9-year-old male quadriplegic, spastic cerebral palsy (CP) patient using low and high lasers in association. BACKGROUND: Various management methods for oral trauma have been suggested, depending on the severity, frequency, and cause of injury, including medication, behavioral techniques, and oral appliances or dental extractions. METHODS: Initially, low-level laser therapy (LLLT) was used on the injured labial tissue measuring 2.2 cm externally and 3.4 cm in the internal mucosal, followed by surgical removal using a CO(2) laser. After 30 days, a significant reduction in injury to the oral tissues was observed, and the region presented normal color and good healing conditions. CONCLUSION: The association of different laser therapies to remove and heal a lip lesion owing to self-injurious behavior was effective and promoted improvement in the patient's quality of life by establishing painless mastication.
