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1.
Acta bioquím. clín. latinoam ; 46(4): 615-623, dic. 2012. ilus, tab, graf
Artigo em Espanhol | LILACS | ID: lil-671968

RESUMO

Durante el tratamiento endodóntico, la irrigación del conducto radicular permite remover los residuos de los túbulos dentinarios. El objetivo de este estudio fue evaluar el contenido de soluciones de irrigación extraídas del conducto radicular luego de la pulpectomía en dientes con pulpitis y con necrosis pulpar, con el fin de determinar aquéllas menos agresivas sobre la dentina radicular. Se trabajó en 80 dientes humanos superiores unirradiculares y con NaClO 1%, EDTA 17%, Ca(OH)2 1%, clorhexidina 0,2% y agua destilada. Se aspiró el contenido de tres irrigaciones y se determinó pH, proteínas totales, hidroxiprolina, calcio y fósforo. El pH de las soluciones no tuvo cambios significativos. La mayor concentración de proteínas se halló en los aspirados con NaClO de dientes con necrosis y con EDTA de pulpitis, revelando mayor número de bandas por electroforesis con esta última solución. El contenido de hidroxiprolina fue mayor con Ca(OH)2 y con clorhexidina, y el de fósforo con EDTA y con NaClO para ambos tipos de dientes. Se detectó calcio con NaClO y clorhexidina. No hubo resultados diferentes entre dientes con pulpitis y con necrosis pulpar en todas las determinaciones químicas. Las soluciones de NaClO y EDTA resultaron eficaces en la eliminación de restos orgánicos de los conductos radiculares. Sin embargo, NaClO eliminó también calcio y fósforo; y la solución de EDTA, fósforo, posiblemente provenientes de la hidroxiapatita y de complejos proteicos de la dentina.


During endodontic treatment, irrigation of the root canal makes it possible to remove remainders of the dentin tubules. The aim of this study was to evaluate the content of extracted irrigation solutions of root canals after pulpectomy in teeth with pulpitis and pulp necrosis, in order to determine those Enless aggressive on root dentine. Work was performed on 80 unirradicular upper human teeth, with 1% NaClO, 17% EDTA, 1% Ca(OH)2, 0.2% chlorhexidine and distilled water. The content of three irrigations was aspired and pH, total proteins, hydroxiproline, calcium and phosphor were determined. pH of the solutions showed no significant changes. A greater protein concentration was obtained with NaClO from teeth with necrosis and with EDTA from teeth with pulpitis; the greatest number of electrophoretic bands were revealed with EDTA. The hydroxiproline content was greater with Ca(OH)2 and with chlorhexidine, and that of phosphor was greater with EDTA and with NaClO for both types of teeth. Calcium was detected with NaClO and chlorhexidine. There were no different results between teeth with pulpitis and those with pulp necrosis in all chemical determinations. NaClO and EDTA solutions were effective in the elimination of organic rests from the root canal. Nevertheless, NaClO also eliminated calcium and phosphor, and the EDTA solution eliminated phosphor, possibly originated from hydroxiapatite and from protein complexes of dentin.


Durante o tratamento do endodóntico, a irrigação do canal radicular permite remover os resíduoss dos túbulos dentinários. O objetivo deste estudo foi avaliar o conteúdo das soluções de irrigação extraídas do canal radicular após a pulpectomia em dentes com pulpite e com necrose pulpar, com a finalidade de determinar aquelas menos agresivas sobre a dentina radicular. O trabalho foi em 80 dentes humanos superiores unirradiculares e com NaClO 1%, EDTA 17%, Ca(OH)2 1%, clorexidina 0.2% e água destilada. Foi aspirado o conteúdo de três irrigações e se determinou pH, proteínas totais, hidroxiprolina, cálcio e fósforo. O pH das soluções não teve mudanças significativas. A maior concentração de proteínas foi encontrada nas aspirações com NaClO de dentes com necrose e o EDTA da pulpite, revelando maior número de faixas por eletroforese com esta última solução. O conteúdo de hidroxiprolina foi maior com Ca(OH)2 e com clorexidina, e o de fósforo com EDTA e com NaClO para ambos os tipos de dentes. Foi detectado cálcio com NaClO e clorexidina. Não houve resultados diferentes entre dentes com pulpite e com necrose pulpar em todas as determinações químicas. As soluções de NaClO e EDTA resultaram eficazes na eliminação de restos orgânicos dos condutos radiculares. Entretanto, NaClO eliminou também cálcio e fósforo; e a solução de EDTA, fósforo, possivelmente provenientes da hidroxiapatita e de complexos proteicos da dentina.


Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Polpa Dentária/química , Tratamento do Canal Radicular , Polpa Dentária , Cavidade Pulpar , Necrose da Polpa Dentária , Endodontia/métodos , Pulpite
2.
Acta bioquím. clín. latinoam ; 46(4): 0-0, dic. 2012. ilus
Artigo em Espanhol | BINACIS | ID: bin-128845

RESUMO

Durante el tratamiento endodóntico, la irrigación del conducto radicular permite remover los residuos de los túbulos dentinarios. El objetivo de este estudio fue evaluar el contenido de soluciones de irrigación extraídas del conducto radicular luego de la pulpectomía en dientes con pulpitis y con necrosis pulpar, con el fin de determinar aquéllas menos agresivas sobre la dentina radicular. Se trabajó en 80 dientes humanos superiores unirradiculares y con NaClO 1%, EDTA 17%, Ca(OH)2 1%, clorhexidina 0,2% y agua destilada. Se aspiró el contenido de tres irrigaciones y se determinó pH, proteínas totales, hidroxiprolina, calcio y fósforo. El pH de las soluciones no tuvo cambios significativos. La mayor concentración de proteínas se halló en los aspirados con NaClO de dientes con necrosis y con EDTA de pulpitis, revelando mayor número de bandas por electroforesis con esta última solución. El contenido de hidroxiprolina fue mayor con Ca(OH)2 y con clorhexidina, y el de fósforo con EDTA y con NaClO para ambos tipos de dientes. Se detectó calcio con NaClO y clorhexidina. No hubo resultados diferentes entre dientes con pulpitis y con necrosis pulpar en todas las determinaciones químicas. Las soluciones de NaClO y EDTA resultaron eficaces en la eliminación de restos orgánicos de los conductos radiculares. Sin embargo, NaClO eliminó también calcio y fósforo; y la solución de EDTA, fósforo, posiblemente provenientes de la hidroxiapatita y de complejos proteicos de la dentina.(AU)


During endodontic treatment, irrigation of the root canal makes it possible to remove remainders of the dentin tubules. The aim of this study was to evaluate the content of extracted irrigation solutions of root canals after pulpectomy in teeth with pulpitis and pulp necrosis, in order to determine those Enless aggressive on root dentine. Work was performed on 80 unirradicular upper human teeth, with 1% NaClO, 17% EDTA, 1% Ca(OH)2, 0.2% chlorhexidine and distilled water. The content of three irrigations was aspired and pH, total proteins, hydroxiproline, calcium and phosphor were determined. pH of the solutions showed no significant changes. A greater protein concentration was obtained with NaClO from teeth with necrosis and with EDTA from teeth with pulpitis; the greatest number of electrophoretic bands were revealed with EDTA. The hydroxiproline content was greater with Ca(OH)2 and with chlorhexidine, and that of phosphor was greater with EDTA and with NaClO for both types of teeth. Calcium was detected with NaClO and chlorhexidine. There were no different results between teeth with pulpitis and those with pulp necrosis in all chemical determinations. NaClO and EDTA solutions were effective in the elimination of organic rests from the root canal. Nevertheless, NaClO also eliminated calcium and phosphor, and the EDTA solution eliminated phosphor, possibly originated from hydroxiapatite and from protein complexes of dentin.(AU)


Durante o tratamento do endodóntico, a irrigaþÒo do canal radicular permite remover os resíduoss dos túbulos dentinários. O objetivo deste estudo foi avaliar o conteúdo das soluþ§es de irrigaþÒo extraídas do canal radicular após a pulpectomia em dentes com pulpite e com necrose pulpar, com a finalidade de determinar aquelas menos agresivas sobre a dentina radicular. O trabalho foi em 80 dentes humanos superiores unirradiculares e com NaClO 1%, EDTA 17%, Ca(OH)2 1%, clorexidina 0.2% e água destilada. Foi aspirado o conteúdo de trÛs irrigaþ§es e se determinou pH, proteínas totais, hidroxiprolina, cálcio e fósforo. O pH das soluþ§es nÒo teve mudanþas significativas. A maior concentraþÒo de proteínas foi encontrada nas aspiraþ§es com NaClO de dentes com necrose e o EDTA da pulpite, revelando maior número de faixas por eletroforese com esta última soluþÒo. O conteúdo de hidroxiprolina foi maior com Ca(OH)2 e com clorexidina, e o de fósforo com EDTA e com NaClO para ambos os tipos de dentes. Foi detectado cálcio com NaClO e clorexidina. NÒo houve resultados diferentes entre dentes com pulpite e com necrose pulpar em todas as determinaþ§es químicas. As soluþ§es de NaClO e EDTA resultaram eficazes na eliminaþÒo de restos orgÔnicos dos condutos radiculares. Entretanto, NaClO eliminou também cálcio e fósforo; e a soluþÒo de EDTA, fósforo, possivelmente provenientes da hidroxiapatita e de complexos proteicos da dentina.(AU)

3.
Acta Odontol Latinoam ; 22(3): 207-14, 2009.
Artigo em Inglês | MEDLINE | ID: mdl-20302220

RESUMO

Success in endodontics depends largely on the activity of the irrigation solutions used during canal cleaning and shaping. Sometimes the irrigation solutions should act deep within the dentin, particularly in cases of chronic infectious lesions, in which it has been found that germs can lodge in the depths of the dentinal tubules. The aim of this study was to compare the penetration of methylene blue in root dentin in instrumented teeth irrigated with different solutions, considering the apical, middle and cervical thirds. Single-rooted teeth were instrumented, irrigated, flooded with 2% methylene blue, washed and cut into thirds. Dye penetration in the dentin was measured by means of two procedures. Linear measurement: maximum dye penetration was recorded towards the four surfaces of each third. Area measurement: the stained surface of each third was measured on digitalized images. On analyzing the data with Friedman and Kruskall Wallis tests, it was found that there was greater penetration of methylene blue in the coronary third in all experimental groups, followed by middle and apical thirds. The mean values for the different groups using the linear method were EDTA 17%: 71.69% - 60.10% - 34.55%. NaOCl 2.5%: 54.04% - 41.79% - 27.53%. CHX 0.2%: 44.28%, 37.58%, 17.80%; and using the area method the mean values were: 17% EDTA: 61.52%, 45.44%, 27.08%. 2.5% NaOCl: 38.15%, 30.77%, 14.60%. 0.2% CHX: 40.95%, 35.46%, 12.27%,for the cervical, middle and coronary thirds respectively. Dye penetration was greatest with 17% EDTA, followed by 2.5% NaOCl and 0.2% CHX, whereas none was observed when distilled water was used.


Assuntos
Dentina , Azul de Metileno , Preparo de Canal Radicular , Animais , Difusão , Cães , Irrigantes do Canal Radicular , Soluções , Irrigação Terapêutica
4.
Artigo em Inglês | MEDLINE | ID: mdl-12847451

RESUMO

OBJECTIVE: Calcium hydroxide, Ca(OH)(2), is a common intracanal medicament. Ca(OH)(2) powder can be mixed with different vehicles and used as a paste for temporary intracanal treatment. The vehicle may influence the dissociation of calcium hydroxide into ions. We sought to evaluate the level of pH and to quantitatively estimate the release of proteins, hydroxyproline, and phosphorus from pieces of radicular dentin kept in different Ca(OH)(2) solutions. STUDY DESIGN: Twenty-eight extracted incisors were maintained for 35 days in Ca(OH)(2) aqueous solutions prepared in chlorhexidine digluconate, propylene glycol (PG), anesthetic solution, camphorated monochlorophenol (CMCP), and CMCP-PG. The control solution contained Ca(OH)(2) without vehicle. RESULTS: The pH values changed little during the experiment. The concentrations of proteins, hydroxyproline, and phosphorus rose for all the solutions under study. Statistical analysis of the data from the control and the experimental groups revealed an increase in the concentration of proteins when chlorhexidine, anesthetic solution, and PG were used; a rise in hydroxyproline levels when CMCP-PG, CMCP, and PG solutions were used; and an increase in phosphorus when PG and chlorhexidine vehicles were used. CONCLUSION: The test solutions with the root dentin remained alkaline. A release of proteins, hydroxyproline, and phosphorus was observed.


Assuntos
Hidróxido de Cálcio/farmacologia , Dentina/efeitos dos fármacos , Irrigantes do Canal Radicular/farmacologia , Análise de Variância , Anestésicos Locais/química , Anestésicos Locais/farmacologia , Anti-Infecciosos Locais/química , Anti-Infecciosos Locais/farmacologia , Hidróxido de Cálcio/química , Cânfora/química , Cânfora/farmacologia , Carticaína/química , Carticaína/farmacologia , Clorexidina/química , Clorexidina/farmacologia , Clorofenóis/química , Clorofenóis/farmacologia , Dentina/química , Combinação de Medicamentos , Humanos , Concentração de Íons de Hidrogênio , Hidroxiprolina/análise , Incisivo , Veículos Farmacêuticos/química , Veículos Farmacêuticos/farmacologia , Fósforo/análise , Propilenoglicol/química , Propilenoglicol/farmacologia , Proteínas/análise , Análise de Regressão , Irrigantes do Canal Radicular/química
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