RESUMO
Reversed-phase liquid chromatography on a C18 bonded silica with water-alcohol mixtures containing decylamine as the mobile phase was investigated for its applicability to the separation of chlorpromazine, imipramine and their metabolites. The addition of decylamine to the mobile phase was found to be necessary in order to obtain symmetrical peaks. The influence of the decylamine and ethanol concentrations and of the pH of the mobile phase was investigated and these parameters were found to be useful for the adjustment of the retention. The phase system developed was found to be suitable for the required separation. The phase system was applied to the analysis of imipramine and its metabolites, desmethylimipramine and didesmethylimipramine, and desmethylchlorpromazine in human plasma.
Assuntos
Clorpromazina/isolamento & purificação , Cromatografia Líquida de Alta Pressão/métodos , Imipramina/isolamento & purificação , Clorpromazina/sangue , Humanos , Imipramina/sangueAssuntos
Chumbo/sangue , Fatores Etários , Cádmio/sangue , Pré-Escolar , Exposição Ambiental , Poluição Ambiental , Humanos , Manganês/sangue , Metalurgia , Países Baixos , Porfirinas/sangue , Zinco/sangueRESUMO
Epidemiological studies on metal levels in blood were carried out. Evidence suggested the existence of a different trend in serum-iron levels in relation to blood lead levels in nonoccupationally exposed males (negative trend) and females (positive trend). There was a positive relation between blood lead levels and blood manganese levels in children and occupationally exposed male workers. Blood metal levels increased with age in young children. There was evidence for an influence of socioeconomic class on blood lead levels in children. A direct and an indirect effect of smoking was found on blood lead levels and on the activity of delta-aminolevulinic acid dehydratase activity in erythrocytes in adult nonoccupationally exposed females.
Assuntos
Chumbo/sangue , Metais/sangue , Adolescente , Adulto , Envelhecimento , Criança , Feminino , Humanos , Masculino , Manganês/sangue , Pessoa de Meia-Idade , Doenças Profissionais/sangue , Doenças Profissionais/induzido quimicamente , Fumar/sangue , Fatores Socioeconômicos , Espectrofotometria AtômicaAssuntos
Cádmio/sangue , Cobre/sangue , Chumbo/sangue , Zinco/sangue , Humanos , Espectrofotometria Atômica/métodosRESUMO
PIP: Blood lead (PbB) and serum iron (Fe-S) levels were studied in 148 nonoccupationally exposed 19-21 year old student volunteers: 57 males, 47 non-oral-contraceptive (OC)-using females and 44 regular OC-using females. PbB levels ranged from 46 to 238 parts/billion. Free-erythrocyte-porphyrins (FEPs), Fe-S, hemoglobin, hematocrit, total Fe-binding-capacity (TIBC), and unsaturated-IBC were measured. A negative correlation was seen between PbB and Fe-S in males (r = -.36, p .01). Females had a significantly higher FEP level than males at the same PbB level (p .05). OC users had significantly higher Fe-S levels (p .0005) and TIBC (p .005) than non-OC users. A 2nd group of 51 males also showed a negative trend in the relationship between PbB and Fe-S. 2 hypotheses are suggested to explain this negative relationship: 1) competition between iron and lead in regard to the metal-binding capacity of some serum proteins, and 2) feedback inhibition by heme on the iron transport through the erythrocyte precursor membrane.^ieng