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Vet Parasitol ; 214(1-2): 174-7, 2015 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-26414906

RESUMO

Trypanosoma vivax affects cattle herds in Africa and Americas and has been spreading rapidly in Brazil, through introduction of animals with subclinical infections and without apparent parasitemia, which makes its diagnosis challenging. PCR and LAMP are effective in detecting the presence of T. vivax DNA in situations of low parasitemia. LAMP is simpler and faster technique than PCR, and can be performed in the field, with limited resources. In this study, the capacities of conventional PCR and LAMP for detecting T. vivax in bovine blood samples classified as aparasitemic were evaluated. The capacity of conventional PCR (56.25%) for detecting positive samples was lower than that of LAMP (93.73%). This may influence the choice of screening tests for cattle herds infected with T. vivax.


Assuntos
Doenças dos Bovinos/parasitologia , Técnicas de Amplificação de Ácido Nucleico , Parasitemia/veterinária , Trypanosoma vivax/isolamento & purificação , Tripanossomíase Africana/veterinária , Animais , Bovinos , Doenças dos Bovinos/sangue , Doenças dos Bovinos/diagnóstico , Técnicas de Amplificação de Ácido Nucleico/economia , Técnicas de Amplificação de Ácido Nucleico/métodos , Tripanossomíase Africana/diagnóstico , Tripanossomíase Africana/parasitologia
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