RESUMO
Ultrasound speed and impedance microscopy was developed in order to develop in vivo imaging system. The sound speed mode realized non-contact high resolution imaging of cultured cells. This mode can be applied for assessment of biomechanics of the cells and thinly sliced tissues. The impedance mode visualized fine structures of the surface of the rat's brain. This mode can be applied for intra-operative pathological examination because it does not require slicing or staining.
Assuntos
Aumento da Imagem/instrumentação , Interpretação de Imagem Assistida por Computador/instrumentação , Microscopia Acústica/instrumentação , Processamento de Sinais Assistido por Computador/instrumentação , Desenho de Equipamento , Análise de Falha de Equipamento , Aumento da Imagem/métodos , Interpretação de Imagem Assistida por Computador/métodos , Microscopia Acústica/métodos , Reprodutibilidade dos Testes , Sensibilidade e EspecificidadeRESUMO
Our goal is to achieve the precise quantitative imaging of tissue elasticity in clinical settings. In the present study, we measured basic ultrasonic characteristics of atherosclerosis by two-dimensional (2D) intravascular tissue velocity imaging. Radio-frequency (RF) signal from a clinically used IVUS apparatus was digitized at 500 MSa/s and stored in a workstation. First, the correlation coefficient between two consecutive frames was calculated in the rotational direction and the rotational disuniformity was corrected to obtain the maximum correlation coefficient. Then, the polar coordinate images were converted into rectangular coordinate images and the images were divided into 64 by 64 square shaped regions of interest (ROIs). The correlation and displacement of the ROIs between the consecutive two frames were calculated by template matching method. Two-dimensional tissue velocity was defined as the vectors of displacement of ROI with 0.7 and more correlation. IVUS studies were performed in directional coronary atherectomy (DCA) procedures. The specimens excised by DCA were stained with Elastica-Masson's trichrome staining and CD68 immunochemical staining. Eleven cases (including two no re-flow cases and one perforation case) were intraoperatively observed by IVUS and the specimens obtained by DCA were observed by optical microscopy. The specimen from homogeneous 2D strain was collagen dominant fibrosis and the specimen from a lesion with complex vectors contained CD68 positive cells and degenerated collagen fibers, which indicated the plaque was vulnerable.
