RESUMO
Post-anesthesia anaphylactic reactions or those seen during drug provocation tests with a systemic clinical reaction may be confirmed by the sequential release into blood of plasma histamine, tryptase and leukotriene C4 and into urine of urinary methylhistamine and leukotriene E4.
Assuntos
Anafilaxia/urina , Hipersensibilidade a Drogas/urina , Leucotrieno E4/urina , Acetaminofen/efeitos adversos , Anafilaxia/sangue , Anafilaxia/induzido quimicamente , Anestésicos/efeitos adversos , Aspirina/efeitos adversos , Biomarcadores , Quimases , Hipersensibilidade a Drogas/sangue , Hipersensibilidade a Drogas/etiologia , Histamina/sangue , Humanos , Leucotrieno C4/sangue , Complicações Pós-Operatórias/induzido quimicamente , Complicações Pós-Operatórias/urina , Serina Endopeptidases/sangue , TriptasesRESUMO
A protocol has been produced for the study of anaphylactic accidents that occur post-operatively that allows definition of the anaphylactic origin, and so reactions that are mediated by IgE in post-operative accidents. This protocol occurs in two stages, the first is done in the minutes and hours that follow the anaphylactic accident, and the second a month or 6 weeks afterwards. At first, we evaluate the sequential study of the liberation of the mediators of anaphylaxis, plasma histamine, serum tryptase, urinary methylhistamine and, more recently, leucotriene E4. The second study is devoted to reactions that are mediated by IgE, essentially, specific serum IgE, tests of activation of basophils by flow cytometry, measurement of leucotriene C4 and skin tests. A study on 16 subjects has evaluated and validated the protocol and shown a significant level of correspondence of results between the sequential measurement of mediators on one hand and on the other the search for IgE-mediated reactions every time that there was an anaphylactic reaction.
Assuntos
Anafilaxia/induzido quimicamente , Anestésicos/efeitos adversos , Hipersensibilidade a Drogas/etiologia , Relaxantes Musculares Centrais/efeitos adversos , Complicações Pós-Operatórias/induzido quimicamente , Adolescente , Adulto , Idoso , Anafilaxia/imunologia , Anafilaxia/metabolismo , Basófilos/metabolismo , Criança , Quimases , Protocolos Clínicos , Creatina/urina , Hipersensibilidade a Drogas/imunologia , Hipersensibilidade a Drogas/metabolismo , Feminino , Gelatina/efeitos adversos , Liberação de Histamina , Humanos , Imunoglobulina E/sangue , Imunoglobulina E/imunologia , Leucotrieno C4/sangue , Leucotrieno E4/urina , Masculino , Metilistaminas/urina , Pessoa de Meia-Idade , Substitutos do Plasma/efeitos adversos , Complicações Pós-Operatórias/imunologia , Complicações Pós-Operatórias/metabolismo , Serina Endopeptidases/sangue , Testes Cutâneos , Succinatos/efeitos adversos , TriptasesRESUMO
Food allergy is becoming more frequent, with 6% of asthmatics reporting an isolated food allergy, and 5 to 6% of atopic dermatitis patients also have either a single or multiple true food allergy. There is value in immuno-biological diagnosis by: Measurement of total serum IgE. Measurement of mono-allergen-specific IgE, following a measurement by a multi-allergen of the Trophatope type. A study of elimination of foods for 2 or 3 months followed by their re-introduction. Oral provocation tests in a hospital environment under clinical control and subsequent measurement of the mediators:-Plasma histamine, tryptase, and urinary methylhistamine to give proof of responsibility of the food allergen. Nowadays, it is perfectly possible to include in diagnosis the new technologies of the test of activation of basophils/or lymphocytes by means of flow cytometry.
Assuntos
Hipersensibilidade Alimentar/diagnóstico , Adolescente , Adulto , Idoso , Animais , Especificidade de Anticorpos , Asma Induzida por Exercício/etiologia , Teste de Degranulação de Basófilos , Criança , Pré-Escolar , Dermatite Alérgica de Contato/complicações , Dieta , Ovos/efeitos adversos , Feminino , Hipersensibilidade Alimentar/complicações , Hipersensibilidade Alimentar/dietoterapia , Hipersensibilidade Alimentar/imunologia , Humanos , Imunoglobulina E/sangue , Imunoglobulina E/imunologia , Ativação Linfocitária , Masculino , Carne/efeitos adversos , Pessoa de Meia-Idade , Nefrose Lipoide/etiologia , Hipersensibilidade Respiratória/complicações , Testes Cutâneos , Solanum tuberosum/efeitos adversos , SuínosRESUMO
Food allergy is becoming more frequent, with 6% of asthmatics reporting an isolated food allergy, and 5 to 6% of atopic dermatitis patients also have either a single or multiple true food allergy. There is value in immuno-biological diagnosis by: Measurement of total serum IgE. Measurement of mono-allergen-specific IgE, following a measurement by a multi-allergen of the Trophatope type. A study of elimination of foods for 2 or 3 months followed by their re-introduction. Oral provocation tests in a hospital environment under clinical control and subsequent measurement of the mediators: Plasma histamine, tryptase, and urinary methylhistamine, to give proof of responsibility of the food allergen. Nowadays, it is perfectly possible to include in diagnosis the new technologies of the test of activation of basophils/or lymphocytes by means of flow cytometry.
Assuntos
Hipersensibilidade Alimentar/diagnóstico , Animais , Asma/imunologia , Asma Induzida por Exercício/imunologia , Ovos , Feminino , Humanos , Masculino , Leite/imunologia , Nefrose Lipoide/imunologiaAssuntos
Anafilaxia/etiologia , Hipersensibilidade a Drogas/complicações , Hipersensibilidade Alimentar/complicações , Frutas/efeitos adversos , Látex/efeitos adversos , Verduras/efeitos adversos , Alérgenos , Criança , Reações Cruzadas , Feminino , Hipersensibilidade Alimentar/diagnóstico , Frutas/imunologia , Humanos , Imunoglobulina E/imunologia , Testes Cutâneos , Verduras/classificação , Verduras/imunologiaRESUMO
Methylhistamine, histamine's metabolite, was measured in urine by radio-immuno-assay in 79 provocation test. Six of them were positive with clinical symptoms. All of the six were associated with a significant increase of urinary methylhistamine (UMH). Therefore, there is a good correlation between positive provocation tests and increase of UMH level. In these cases, the severity of clinical symptoms is related to the increase of U.M.H.