RESUMO
In a laboratory fermentor, the growth of Streptomyces aureofaciens on corn meal extract and its production of antibiotic was investigated. Weak biosynthesis of the antibiotic started after 12 hours of incubation, when phosphorus depletion in the medium occurred. During the third and fourth day of fermentation about 80.4% of the antibiotic was produced. The relationship between growth, antibiotic formation, and the uptake of both sugar and nitrogen was also studied. A chromatogram, showing the types of sugar present during fermentation period is given. Improvement of antibiotic accumulation either by adding sugar at the end of the logarithmic phase of growth or by determining the rate of aeration is also discussed.
Assuntos
Antibacterianos/biossíntese , Streptomyces aureofaciens/metabolismo , Bioensaio , Metabolismo dos Carboidratos , Meios de Cultura , Fermentação , Glucose/farmacologia , Concentração de Íons de Hidrogênio , Nitrogênio/metabolismo , Oxigênio/farmacologia , Fósforo/metabolismo , Streptomyces aureofaciens/crescimento & desenvolvimento , Zea maysRESUMO
Complete extraction of S. aureofaciens lD13 antibiotic was achieved by adding n-butanol to the clarified culture filtrate (v/2v) at pH 8.0. Using the column chromatography technique, 85.7% of the initial amount of the antibiotic was obtained in a purified form. Data of the Rf values of the antibiotic in different organic solvents revealed that it belongs to the tetracycline group. The antibiotic was chromatographically analyzed, using the thin-layer technique. UV and IR spectra, optical rotation, melting point as well as 15 colour reactions were also determined.
