Prevention Strategies and Research Methods of TCM for Primary Osteoporosis / 中国中医药信息杂志

Chronic non-infectious diseases (hereinafter referred to as "chronic disease") have become a serious public health problem which affect the physical and mental health. Its prevention and control is urgent. Primary osteoporosis is a common chronic disease, which has complex causes, long duration, protracted illness, and poor prognosis, and is often accompanied by fracture and other serious complications. Control strategies of TCM are identify the risk factors of TCM and assess the risk of illness; follow the clinical practice guidelines in Western medicine and TCM and attach great importance to the high-quality evidence of TCM; collect TCM information of study population and established the biobank through long-term follow-up study. The specific method was: according to the TCM theory of "treat pre-disease", the clinical epidemiological investigation and experimental research methods are combined to carry out disease prevention and control work. In the stage of normal bone mass or osteopenia, the clinical efficacy of TCM program in improving bone mineral density were mainly evaluated. In the stage of osteoporosis (fracture has not yet occurred), the main object was to verify efficacy and safety of TCM or integrative programs preventing the fracture. In osteoporotic fracture phase, the focus was to evaluate whether the combination therapy programs could promote healing, prevent recurrence of the fracture and improve the quality of life, thus reducing morbidity and mortality.

Scene-trait coping style of military rescuers in Wenchuan earthquake / 中国应用生理学杂志

<p><b>OBJECTIVE</b>This study aimed to explore the scene-trait coping style of military rescuers in Wenchuan earthquake in an effort to provide scientific evidences for mental intervention program for Chinese military personnel.</p><p><b>METHODS</b>By cluster sampling, a total of 151 military rescuers and 331 control servicemen were administered the military personnel scene-trait coping style scale (MPSTCSS).</p><p><b>RESULTS</b>All active coping factor scores, and passive coping factor scores of affection, health and economy in the rescuer group were significantly higher than those in control group (P < 0.05). The 21-above age subgroup, the 3-year plus service subgroup, and the officer subgroup had significantly higher active coping factor scores on military tasks, military experience and personal development than those of the 21-below age subgroup, 3-year minus service subgroup and the soldier subgroup, respectively (P < 0.05 or P < 0.01).</p><p><b>CONCLUSION</b>The earthquake relief servicemen can cope with stressful situations better than control group by taking active coping style. The officers, servicemen older than 21 years, and servicemen with more service duration than 3 years could usually take active coping style.</p>

Psychosomatic health of military personnel recruited from minorities and influential factors thereof / 解放军医学杂志

Objective To evaluate the psychosomatic health of military personnel recruited from minorities, and explore influential factors thereof. Methods A total of 11 362 soldiers from several military commands of infantry, navy and air force recruited from different nationalities were selected by random cluster sampling method, and then surveyed with the aid of Chinese Psychosomatic Health Scale. The collected data were analyzed by t-test, correlation analysis, and multivariate stepwise regression analysis. Results Generally, the factor scores of respiratory system, cardiovascular system, digestive system and anxiety were significantly lower in personnel recruited from ethnic minorities than those in personnel recruited from Han nationality (P0.05), while in 1990s and 2000-2009, the factor scores of mental health, physical health and total psychosomatic health in minority group were lower than those in Han group (P<0.05 or P<0.01). Statistical analysis showed that the factor scores of mental health, physical health and total psychosomatic health were significantly negatively correlated with time period and nationality (P<0.01). All time period and nationality factor entered into the regression equation of mental health, physical health and total psychosomatic health (P<0.05 or P<0.01). Conclusions No significant difference of psychosomatic health was found between minority group and Han military personnel in 1980s, while after 1990 the mental health, physical health and total psychosomatic health in minority personnel have become better than those in Han personnel.

Tumor suppressor genes on frequently deleted chromosome 3p in nasopharyngeal carcinoma / 癌症

Nasopharyngeal carcinoma (NPC) is among the most common malignancies in southern China. Deletion of genomic DNA, which occurs during the complex pathogenesis process for NPC, represents a pivotal mechanism in the inactivation of tumor suppressor genes (TSGs). In many circumstances, loss of TSGs can be detected as diagnostic and prognostic markers in cancer. The short arm of chromosome 3 (3p) is a frequently deleted chromosomal region in NPC, with 3p21.1-21.2 and 3p25.2-26.1 being the most frequently deleted minimal regions. In recent years, our research group and others have focused on the identification and characterization of novel target TSGs at 3p, such as RASSF1A, BLU, RBMS3, and CHL1, in the development and progression of NPC. In this review, we summarize recent findings of TSGs at 3p and discuss some of these genes in detail. A better understanding of TSGs at 3p will significantly improve our understanding of NPC pathogenesis, diagnosis, and treatment.

The prognostic significance of peripheral T-lymphocyte subsets and natural killer cells in patients with colorectal cancer.

BACKGROUND/AIMS: To evaluate the prognostic significance of preoperative T-lymphocyte subsets ad natural killer (NK) cells and their correlan with other prognostic factors in patients with colorectal cancer who underwent surgical treatment. METHODOLOGY: The numbers of T-lymphocytes and NK cells in the peripheral blood of patients with colorectal carcinoma were counted by flow cytometry, and the correlations between indicators of cellular immunity and clinicopathological characteristics and the prognosis of patients after surgical treatment were analyzed by univariate and multivariate analyses. RESULTS: In the multivariate regression analysis, all potential prognostic factors essentially reflected TNM stage, and tumor resection, NK lymphocyte counts, and peripheral CD3+ and CD4+/CD8+ counts were significant (p < 0.05) independent prognostic indicators of overall survival; patients with higher CD3+, CD4+/CD8+, and NK levels had longer overall survival rates than those with lower CD3+, CD4+/CD8+, and NK levels. CONCLUSION: Measurement of cellular immunity in the peripheral blood of patients with colorectal cancer allowed us to identify associations between immune status and clinical outcomes.

Expression of annexin II in patients with hematologic malignancies / 中国实验血液学杂志

This study was purposed to investigate the expression of annexin II in patients with hematologic malignancies and its role in genesis and development of hematologic malignancies. The expression levels of annexin II in bone marrow cells from untreated 81 patients with acute leukemia, 6 patients with MM and 20 patients with iron deficiency anemia were detected by real-time PCR assay. The results showed that the expression of annexin II mRNA significantly increased in M(3) patients as compared with others, the expression of annexin II gene in groups M(5), MM, M(4) were higher than that of other groups except M(3) group, and there were no significant difference in expression of annexin II gene between M(1) + M(2) groups and controls. It is concluded that the expression of annexin II gene significantly increased in patients M(5), M(4), MM, who showed higher ratio of infiltration than other patients. It is inferred that the annexin II participates in invasion and infiltration of hematologic malignancies probably through enhancing the degradation of extracellular matrix by cells of hematologic malignancies.

Full-length of human papillomavirus type 18L1 gene optimized using the plant preferred codons and synthesized by overlapping PCR / 南方医科大学学报

<p><b>OBJECTIVE</b>The Human papillomavirus type 18L1 (HPV18L1) gene was synthesized by overlapping PCR after optimization using plant preferred codons.</p><p><b>METHODS</b>The gene sequences of HPV18L1 were obtained from GenBank and analyzed using DNAMAN, Lasergene, Vector NTI and BLAST. The target sequence was selected and modified using plant preferred codons by the Synthetic Gene Designer and JCat (Java Codon Adaptation Tool) with the addition of a His-tag to the C-terminus to construct the full-length modified HPV18L1 (mHPV18L1). mHPV18L1 was divided into 5 large segments, namely LS1 to LS5, with sizes ranging from 204 to 477 bp. Forty-three small oligonucleotide fragments with sizes of 57-59 bp and 6 pairs of primers were designed and synthesized. mHPV18L1 was amplified by overlapping PCR and subcloned into pMD18-T vector. The recombinant plasmid was identified by restriction enzymes digestion and sequencing.</p><p><b>RESULTS</b>mHPV18L1 was successfully assembled using overlapping PCR. The results of digestion with restriction enzymes and PCR amplification confirmed that the recombinant vector pMD18T- mHPV18L1 contained the inserts with expected size of 1749 bp. mHPV18L1 sequence was confirmed by sequencing.</p><p><b>CONCLUSION</b>mHPV18L1 with plant preferred codons and the recombinant vector pMD18T- mHPV18L1 have been obtained.</p>