RESUMO
Solid-phase organic synthesis is now a prevalent activity in drug discovery. In keeping with this keen interest is the need to develop reliable automated synthesis instrumentation as well as polymeric supports and linkers suitable for the full range of organic synthesis applications. In this paper, we review our activities in the development of new and enabling tools for automated chemical synthesis, including the following: (i) new solid supports such as ArgoGel (PS-PEG-based) and Argo-X203 (PS-based); and (ii) the Nautilus 2400 system, a fully closed and inert automated chemistry development workstation. Selected chemistry optimization and synthesis examples performed on the Nautilus and new solid supports will be described.
Assuntos
Bioquímica/instrumentação , Bioquímica/métodos , Resinas Vegetais/síntese química , Automação , Indicadores e Reagentes , Espectroscopia de Ressonância Magnética , Polietilenoglicóis/síntese química , Poliestirenos/síntese química , Fatores de TempoRESUMO
Many mammalian cells grown in culture excrete ammonia, which, when it accumulates, limits cell growth and reduces product synthesis. Common tactics for minimizing the effects of ammonia accumulation are uneconomical, requiring large quantities of media and incurring high capital costs. Solution-diffusion membranes were investigated for ammonia removal, and a supported-gas membrane was identified that could be used to remove ammonia rapidly to well below inhibitory levels. Medium treated using this membrane was reused to culture baby-hamster kidney cells, resulting in a cell growth rate that was essentially the same as that for cells grown in fresh medium.
