Potential Effect of Dietary Supplementation of Tannin-Rich Forage on Mitigation of Greenhouse Gas Production, Defaunation and Rumen Function.

This experiment evaluated the effect of including Acacia mearnsii leaves in a high-fiber diet (corn stover), on ruminal degradation kinetics, digestibility, microbial biomass production, and gas, CH4, and CO2 production. Four experimental diets were tested, including a control with 100% corn stover (T1), and three additional diets with corn stover supplemented at 15% A. mearnsii leaves (T2), 30% A. mearnsii leaves (T3) and 45% of A. mearnsii leaves (T4). The highest dry matter in situ degradation (p ≤ 0.001) and in vitro digestibility (p ≤ 0.001) was found in T1 (80.6 and 53.4%, respectively) and T2 (76.4 and 49.6%, respectively) diets. A higher population of holotrich and entodiniomorph ruminal protozoa was found (p = 0.0001) in T1 at 12 and 24 h. Diets of T1 and T2 promoted a higher (p = 0.0001) microbial protein production (314.5 and 321.1 mg/0.5 g DM, respectively). Furthermore, a lower amount of CH4 was found (p < 0.05) with T2, T3 and T4. It is concluded that it is possible to supplement up to 15% of A. mearnsii leaves (30.5 g TC/kg DM) in ruminant's diets. This decreased the population of protozoa (holotrich and entodiniomorph) as well as the CH4 production by 35.8 and 18.5%, respectively, without generating adverse effects on the ruminal degradation kinetics, nutrient digestibility and microbial protein production.

Use of tannin extract as a strategy to reduce methane in Nellore and Holstein cattle and its effect on intake, digestibility, microbial efficiency and ruminal fermentation.

This study was carried out to evaluate the use of tannin extract from Acacia mearnsii as a strategy to reduce methane (CH4 ) in two distinct cattle genotypes and its effect on intake, digestibility, microbial efficiency and ruminal fermentation. Four Nellore (Bos indicus) and four Holstein (Bos taurus) dry cows fitted with rumen cannula were assigned to two 4 × 4 Latin square design, in a 2 × 4 factorial arrangement, where each genotype represented a square receiving four tannin levels (commercial extract of A. mearnsii) in the diet (0%, 0.5%, 1.0% and 1.5% of dry matter). Tannin levels used did not cause a reduction in feed intake or rumen passage rate for both genotypes (p > 0.05), although there was a linear reduction in the degradation rate and ruminal disappearance of diet (p < 0.05). The increase in tannin levels reduced the amount of entodiniomorph protozoa in the Nellore cattle (p < 0.05). There was no change in N retention or microbial efficiency (p > 0.05), despite the linear reduction of nutrient digestibility and the synthesis of microbial nitrogen (p < 0.05). The ruminal CH4 production was reduced (p < 0.05) without reducing the short-chain fatty acid production. The threshold of 0.72% of tannin in the diet was estimated as the starting point for the reduction of ruminal CH4 production with long-term efficacy. Therefore, the use of low levels of tannin extract from A. mearnsii is a potential option to manipulate rumen fermentation in Nellore and Holstein cattle and needs to be further investigated.

Anatomical characterization of black wattle wood for the pulp and paper production / Caracterização anatômica do lenho de acácia-negra para produção de celulose e papel

The growing worldwide demand in the pulp market has fostered research that evaluates alternative fiber sources with specific characteristics that attend the needs of the consumer market, with a view to add value to the final product and reduce production costs. Acacia mearnsii De Wild wood is a by-product of the extraction of tannin from the trunk bark, used for firewood, charcoal and pellets. However, its wood is still poorly studied, especially its anatomical characteristics, which can provide important information about its industrial potential. This study evaluated the anatomical characteristics of Acacia mearnsii wood for the production of pulp and paper. Ten trees at approximately seven years old were cut down, five from the seed production area (SPA) and five from the clonal population area (CPA). From each tree, one trunk disc was sectioned at the diameter at breast height (DBH), resulting in 10 (ten) wood samples. From each disc, a sapwood specimen was made oriented in the tangential longitudinal, radial longitudinal and transversal planes, for later obtaining the anatomical cuts and the macerates. The anatomical description of the wood followed the recommendations of the International Association of Wood Anatomists-IAWA. From the dimensions of the fibers, their quality evaluation ratios for the production of pulp and paper were calculated. Results obtained from the anatomical characterization allowed to conclude that the Acacia mearnsii woods from SPA and CPA are indicated as a source of raw material for the pulp and paper production.

A crescente demanda mundial do mercado de polpa celulósica tem fomentado pesquisas que avaliem fontes de fibras alternativas com características específicas que atendam às necessidades do mercado consumidor, visando agregar valor ao produto final e reduzir os custos de produção. No estado do Rio Grande do Sul, Brasil, o lenho de Acacia mearnsii De Wild é considerado um subproduto da extração de tanino da casca do tronco, utilizada como lenha, carvão e pellets. No entanto, ainda é pouco estudado, em especial suas características anatômicas, as quais podem fornecer informações importantes sobre suas potencialidades industriais. O presente estudo teve como objetivo avaliar as características anatômicas do lenho de Acacia mearnsii para a produção de celulose e papel. Foram coletadas dez árvores com aproximadamente sete anos de idade, sendo cinco provenientes da área de produção de sementes (APS) e cinco da área de povoamento clonal (APC). De cada árvore, foi seccionado um disco do tronco na altura do DAP, resultando em dez amostras de lenho. De cada disco, foi confeccionado um corpo de prova do alburno, orientado nos planos longitudinal tangencial, longitudinal radial e transversal, para posterior obtenção dos cortes anatômicos e dos macerados. A descrição anatômica do lenho seguiu as recomendações da Associação Internacional de Anatomistas da Madeira-IAWA. A partir das dimensões das fibras, foram calculados os seus índices de avaliação da qualidade para a produção de celulose e papel. Os resultados obtidos da caracterização anatômica permitiram concluir que os lenhos de Acacia mearnsii provenientes de APS e APC são indicados como matérias-primas para a produção de celulose e papel.

Milk fatty acid composition of cows and ewes supplemented with black wattle tannin extract / Composição em ácidos graxos no leite de vacas e ovelhas suplementadas com extrato tanífero de acácia negra

This study evaluated the effects of Acacia mearnsii tannin extract on milk fatty acid profile of dairy ewes and cows. In experiment 1, twenty-four Lacaune ewes received one of the following dietary treatments: control (no tannin extract added to the diet), T30 (30 g tannin extract/kg concentrate), and T40 (40 g extract/kg concentrate). In experiment 2, thirty Jersey cows received either a control diet (no tannin extract added to the diet) or the same diet containing 40 g tannin extract/kg concentrate (T40). Dry matter intake, milk production and milk solids content of ewes and cows were unaffected by tannin supplementation. The cis-9, trans-11 conjugated linoleic acid (CLA) content increased linearly by 21% in milk fat from ewes fed tannin extract, while the C18:2 n-6 (linoleic acid) content tended (P = 0.051) to increase by 13% in cows fed TE. The supplementation with black wattle tannin extract improves the nutritional quality of milk fat of ewes and cows to a small extent, with no adverse effects on performance or gross milk composition.

O objetivo deste trabalho foi avaliar os efeitos da suplementação com extrato tanífero de acácia negra (Acacia mearnsii) sobre o perfil de ácidos graxos no leite de ovelhas e vacas. No experimento 1, vinte e quatro ovelhas Lacaune receberam um dos seguintes tratamentos dietéticos: controle (sem extrato tanífero), T30 (30 g de extrato tanífero/kg concentrado) e T40 (40 g de extrato tanífero/kg concentrado). No experimento 2, trinta vacas Jersey receberam uma dieta controle ou a mesma dieta contendo 40 g de extrato tanífero/kg concentrado (T40). O consumo de matéria seca, a produção de leite e o teor de sólidos do leite das ovelhas e vacas não foram afetados pela suplementação com o extrato tanífero. O teor de ácido linoleico conjugado (CLA) cis-9, trans-11 aumentou em 21% na gordura do leite de ovelhas alimentadas com o extrato tanífero, enquanto o teor de C18:2 n-6 (ácido linoleico) tendeu (P = 0,051) a aumentar em 13% em vacas suplementadas com o extrato. A suplementação com extrato tanífero de acácia negra melhora em pequena escala a qualidade nutricional da gordura do leite de ovelhas e vacas, sem efeitos adversos no desempenho ou na composição bruta do leite.

Influence of Acacia Mearnsii Fodder on Rumen Digestion and Mitigation of Greenhouse Gas Production.

In recent years, the worrying generation of GHG from ruminant production has generated widespread interest in exploring nutritional strategies focused on reducing these gases, presenting the use of bioactive compounds (tannins) as an alternative in the diet. The aim of this research was to determine the effect of the addition of different levels of Acacia mearnsii on ruminal degradation, nutrient digestibility, and mitigation of greenhouse gas production. A completely randomized design with four treatments and six repetitions was used. The treatments were: T1, T2, T3, and T4 diets with, respectively, 0%, 20%, 40%, and 60% A. mearnsii. The rumen degradation kinetic and in vitro digestibility, and the production of gas, CH4, and CO2 were evaluated. In situ rumen degradation and in vitro digestibility of DM and OM showed differences between treatments, with T1 being higher (p < 0.05) in the degradation of the soluble fraction (A), potential degradation (A + B), and effective degradation for the different passage rates in percent hour (0.02, 0.05, and 0.08), compared to the other treatments. Rumen pH did not show differences (p > 0.05) between treatments. The lowest (p < 0.05) gas, CH4, and CO2 production was observed in treatments T1 and T2 with an approximate mean of 354.5 mL gas/0.500 g fermented DM, 36.5 mL CH4/0.500 g fermented DM, and 151.5 mL CO2/0.500 g fermented DM, respectively, compared to treatments T3 and T4. Under the conditions of this study, it was concluded that it is possible to replace traditional forages with up to 20% of A. mearnsii, without observing changes in the production of greenhouse gases with respect to the control treatment (0% of A. mearnsii); however, A. mearnsii is not usable because it significantly decreases rumen degradability of DM and OM, which would considerably affect the production in animals.

The Response Surface Optimization of Supercritical CO2 Modified with Ethanol Extraction of p-Anisic Acid from Acacia mearnsii Flowers and Mathematical Modeling of the Mass Transfer.

A widely disseminated native species from Australia, Acacia mearnsii, which is mainly cultivated in Brazil and South Africa, represents a rich source of natural tannins used in the tanning process. Many flowers of the Acacia species are used as sources of compounds of interest for the cosmetic industry, such as phenolic compounds. In this study, supercritical fluid extraction was used to obtain non-volatile compounds from A. mearnsii flowers for the first time. The extract showed antimicrobial activity and the presence of p-anisic acid, a substance with industrial and pharmaceutical applications. The fractionation of the extract was performed using a chromatographic column and the fraction containing p-anisic acid presented better minimum inhibitory concentration (MIC) results than the crude extract. Thus, the extraction process was optimized to maximize the p-anisic acid extraction. The response surface methodology and the Box-Behnken design was used to evaluate the pressure, temperature, the cosolvent, and the influence of the particle size on the extraction process. After the optimization process, the p-anisic acid yield was 2.51% w/w and the extraction curve was plotted as a function of time. The simulation of the extraction process was performed using the three models available in the literature.

Efeito de IBA e de rizobactéria no enraizamento de miniestacas de Acácia negra / Effect of IBA and rhizobacterium in rooting of Black wattle minicuttings

A acácia negra é uma espécie florestal de expressiva importância econômica na região sul do Brasil e a investigação de métodos de propagação vegetativa para constituição de florestas clonais é de grande valia para aumento da produtividade florestal. O objetivo deste trabalho foi avaliar a ação de promotores de enraizamento na miniestaquia de Acacia mearnsii como alternativa para a propagação vegetativa da espécie. O experimento foi conduzido no mês de julho/2018 (estação de inverno) com uma testemunha, duas concentrações de ácido indol butírico - IBA (2000 e 4000 mg L-1) e duas concentrações do inoculante AzoTotal® (100% e 50%) que contem a bactéria Azospirillum brasilense. Para os tratamentos com IBA, as bases das miniestacas permaneceram imersas em solução hidroalcoólica 1:1 durante 10 segundos e para o inoculante durante 15 minutos. O experimento foi conduzido em casa de vegetação e após 45 dias avaliou-se a porcentagem de miniestacas enraizadas, número de raízes por miniestaca, comprimento das três maiores raízes por miniestaca, porcentagem de miniestacas com calos, porcentagem de sobrevivência das miniestacas, porcentagem de miniestacas com brotação e porcentagem de miniestacas que mantiveram os folíolos. Houve diferença significativa entre os tratamentos para miniestacas enraizadas, número de raízes por miniestaca e comprimento das três maiores raízes, não ocorrendo para as demais variáveis analisadas. A utilização de IBA em 4000 mg L-1mostrou-se mais eficaz (63,7% de enraizamento)para o processo de propagação vegetativa da espécie.(AU)

Black wattle is a forest species of great economic importance in the southern of Brazil. Researches for vegetative propagation methods to constitute clonal forests are effective to increase forest productivity. The goal of this work was to evaluate the action of compounds to promote rooting in minicuttings of Acacia mearnsii as an alternative to vegetative propagation to the species. We conducted the experiment in July/2018 (winter season) with one negative control, two concentrations of indolebutyric acid –IBA (2000 and 4000 mg L-1) and two concentrations of AzoTotal™ (100% e 50%), an inoculant containing Azospirillum brasilense bacterium. In the IBA treatments, the base of minicuttings remained immersed in hydroalcoholicsolution1:1for 10 seconds and for the inoculant treatments during15 minutes. The experiment took place in a greenhouse and after 45 days, we evaluated the percentage of rooted minicutting; number of roots per minicutting; length of the three largest roots perminicutting; percentage of minicuttings with calluses; percentage of minicuttings survival; percentage of minicutting with budding and percentage of minicutting that kept the leaflets. There was significant difference between treatments considering rooted minicuttings, number of roots by minicutting, length of the three largest roots by minicutting and we did not observe differences tothe other variables analyzed. The use of IBA at 4000 mg L-1(63.7% of rooted minicuttings) was more effective for the vegetative propagation process in this species.(AU)

Efeito de IBA e de rizobactéria no enraizamento de miniestacas de Acácia negra / Effect of IBA and rhizobacterium in rooting of Black wattle minicuttings

A acácia negra é uma espécie florestal de expressiva importância econômica na região sul do Brasil e a investigação de métodos de propagação vegetativa para constituição de florestas clonais é de grande valia para aumento da produtividade florestal. O objetivo deste trabalho foi avaliar a ação de promotores de enraizamento na miniestaquia de Acacia mearnsii como alternativa para a propagação vegetativa da espécie. O experimento foi conduzido no mês de julho/2018 (estação de inverno) com uma testemunha, duas concentrações de ácido indol butírico - IBA (2000 e 4000 mg L-1) e duas concentrações do inoculante AzoTotal® (100% e 50%) que contem a bactéria Azospirillum brasilense. Para os tratamentos com IBA, as bases das miniestacas permaneceram imersas em solução hidroalcoólica 1:1 durante 10 segundos e para o inoculante durante 15 minutos. O experimento foi conduzido em casa de vegetação e após 45 dias avaliou-se a porcentagem de miniestacas enraizadas, número de raízes por miniestaca, comprimento das três maiores raízes por miniestaca, porcentagem de miniestacas com calos, porcentagem de sobrevivência das miniestacas, porcentagem de miniestacas com brotação e porcentagem de miniestacas que mantiveram os folíolos. Houve diferença significativa entre os tratamentos para miniestacas enraizadas, número de raízes por miniestaca e comprimento das três maiores raízes, não ocorrendo para as demais variáveis analisadas. A utilização de IBA em 4000 mg L-1mostrou-se mais eficaz (63,7% de enraizamento)para o processo de propagação vegetativa da espécie.

Black wattle is a forest species of great economic importance in the southern of Brazil. Researches for vegetative propagation methods to constitute clonal forests are effective to increase forest productivity. The goal of this work was to evaluate the action of compounds to promote rooting in minicuttings of Acacia mearnsii as an alternative to vegetative propagation to the species. We conducted the experiment in July/2018 (winter season) with one negative control, two concentrations of indolebutyric acid –IBA (2000 and 4000 mg L-1) and two concentrations of AzoTotal™ (100% e 50%), an inoculant containing Azospirillum brasilense bacterium. In the IBA treatments, the base of minicuttings remained immersed in hydroalcoholicsolution1:1for 10 seconds and for the inoculant treatments during15 minutes. The experiment took place in a greenhouse and after 45 days, we evaluated the percentage of rooted minicutting; number of roots per minicutting; length of the three largest roots perminicutting; percentage of minicuttings with calluses; percentage of minicuttings survival; percentage of minicutting with budding and percentage of minicutting that kept the leaflets. There was significant difference between treatments considering rooted minicuttings, number of roots by minicutting, length of the three largest roots by minicutting and we did not observe differences tothe other variables analyzed. The use of IBA at 4000 mg L-1(63.7% of rooted minicuttings) was more effective for the vegetative propagation process in this species.

Physicochemical Quality, Fatty Acid Composition, and Sensory Analysis of Nellore Steers Meat Fed with Inclusion of Condensed Tannin in the Diet.

This study was conducted to test the effect of dietary tannin on the fatty acid profile and sensory attributes of meat from Nellore steers. Thirty-two Nellore bull male were distributed in a completely randomized design and fed diets with condensed tannin extract as follows: 0, 10, 30, and 50 g/kg total DM basis. The physicochemical composition of the meat, lipid oxidation, fatty acid profile, flavor, tenderness, and overall acceptance were evaluated. There was a linear decrease (P ≤ 0.05) on lipid content, tenderness, cooking weight loss, myristic, palmitic, and oleic acids in meat as tannin increased in the diets. The total saturated and monounsaturated fatty acids, the atherogenicity index decreased. However, a linear increase (P ≤ 0.05) was observed for linoleic, linolenic, arachidonic, eicosapentaenoic, and docosapentaenoic acids. The physicochemical characteristic of the meat, such as moisture, ash, and protein contents, water retention capacity, final pH, Warner-Bratzler shear force, collagen, and color indexes (lightness, redness, yellowness, and chrome) did not change with dietary tannin. Also, CLA, n-6:n-3 ratio, Δ9 -desaturase, and elongase activity were not different among diets. In conclusion, condensed tannin linearly increases unsaturated fatty acids and decreases the atherogenicity index of meat; thus, it can be recommended at the highest level (50 g/kg DM) in the diet of Nellore steers. PRACTICAL APPLICATION: Agriculture byproducts plays an important part in the diet of ruminant animals and consequently on food chain and has implications for the composition and quality of the livestock products (milk, meat, and eggs) that people consume. Feeding tannin to steers increases the amount of unsaturated fatty acids and meat tenderness, with a concomitant reduction on saturated fatty acids and the atherogenicity index in meat. Thus, we recommend adding tannin to steer diets to reduce the risk factors for cardiovascular diseases in red meat for human consumption.

The effects of supplementing Acacia mearnsii tannin extract on dairy cow dry matter intake, milk production, and methane emission in a tropical pasture.

The study assessed the effect of Acacia mearnsii tannin extract supplementation grazing dairy cows on dry matter (DM) intake, enteric methane (CH4) emission, and performance. Twelve Holstein cows were divided into two groups and subjected to two treatments that consisted of millet pasture (Pennisetum glaucum L.) plus supplementation with 6 kg of concentrate (750-g/kg ground corn and 250-g/kg soybean meal) including or excluding 120-g tannin extract. The trial design was a double reversal using three periods of 28 days each, with 21 days for the adaption period, and 7 days for sample collection. Herbage intake was measured using the n-alkane technique, and daily CH4 emission was measured with the sulfur hexafluoride tracer gas technique. Individual total DM intake (mean = 17.1 kg/day), herbage DM intake (mean = 11.8 kg/day), and milk production (mean = 19.2 kg/day) were similar between treatments. CH4 emission significantly decreased (32%, P < 0.05) in the animals supplemented with tannin extract, compared to non-supplemented animals. On the other hand, as proportion of DM intake or milk production, methane emission tended to decrease in tannin-supplemented animals. Supplementing dairy cows grazing a millet pasture with 120-g tannin extract reduced daily CH4 emission without affecting animal performance.

Structural, textural and morphological characteristics of tannins from Acacia mearnsii encapsulated using sol-gel methods: Applications as antimicrobial agents.

Tannins from Acacia mearnsii were encapsulated using four different sol-gel methods acid (SGAR), basic (SGBR), silicate (SGSR) and non-hydrolytic (SGNHR) routes. The hybrid materials were analyzed using a set of techniques to characterize their structure, texture and morphology. The antimicrobial performance of the encapsulated materials was evaluated against different microorganisms (Staphylococcus aureus, Escherichia coli, Aspergillus niger and Candida sp.). The data showed that the encapsulation route significantly affects the characteristics of the resulting hybrid materials. Better functional performances were obtained using the silicate route, which produced mesoporous materials with a small surface area (0.96m2g-1) and small particle size (<1nm). These characteristics promoted the gradual release of tannins in an aqueous medium and improved their interactions with microorganisms. Furthermore, the process demonstrated the preservation of tannins after synthesis and increased antimicrobial activity (via a controlled tannin release), as demonstrated by the moderate activity against filamentous fungi and yeast.

Tannin extracts on quality of fresh cut crisp leaf lettuce / Extratos de taninos na qualidade de alface crespa minimamente processada

In the present study, tannin extracts (rinsed or not) were compared to the use of sodium hypochlorite and tap water on fresh cut crisp leaf lettuce stored under modified atmosphere packaging. Effects of these sanitizers on total color difference and microbial levels of the product after sanitization and storage for 9 days at 3°C were evaluated. Performance of rinsed SM(r) tannin extract was comparable to the results of chlorine solution for all the analyzed parameters and; furthermore, that extract presented a high reduction in the initial bacterial count of minimally processed lettuce. However, storage of tannin extracts, did not impart better outcomes than the use of tap water. Therefore, the tannin extract storage SM(r) could be used in washing water to reduce the initial microbiological load, avoiding cross contamination in vegetables minimally processed.(AU)

Extratos de taninos (com e sem enxague) foram comparados com o uso de hipoclorito de sódio e água em alface crespa minimamente processada, sob atmosfera modificada. Foram avaliadas diferença total de cor e análises microbiológicas após a sanitização e durante o armazenamento dos produtos (9 dias à 3°C). O extrato de tanino SM(r) com enxague apresentou resultados semelhantes aos da solução de cloro para todos os parâmetros analisados e, além disso, apresentou uma elevada redução da contagem inicial microbiológica de alface minimamente processada. No entanto, durante o armazenamento, SM(r) não apresentou melhores resultados do que o uso de água. Portanto, SM(r) poderia ser utilizado na desinfecção da água de lavagem das alfaces, reduzindo a microbiota inicial e, assim, impedir a contaminação cruzada durante o processamento mínimo de vegetais.(AU)

Tannin extracts on quality of fresh cut crisp leaf lettuce / Extratos de taninos na qualidade de alface crespa minimamente processada

ABSTRACT: In the present study, tannin extracts (rinsed or not) were compared to the use of sodium hypochlorite and tap water on fresh cut crisp leaf lettuce stored under modified atmosphere packaging. Effects of these sanitizers on total color difference and microbial levels of the product after sanitization and storage for 9 days at 3°C were evaluated. Performance of rinsed SM(r) tannin extract was comparable to the results of chlorine solution for all the analyzed parameters and; furthermore, that extract presented a high reduction in the initial bacterial count of minimally processed lettuce. However, storage of tannin extracts, did not impart better outcomes than the use of tap water. Therefore, the tannin extract storage SM(r) could be used in washing water to reduce the initial microbiological load, avoiding cross contamination in vegetables minimally processed.

RESUMO: Extratos de taninos (com e sem enxague) foram comparados com o uso de hipoclorito de sódio e água em alface crespa minimamente processada, sob atmosfera modificada. Foram avaliadas diferença total de cor e análises microbiológicas após a sanitização e durante o armazenamento dos produtos (9 dias à 3°C). O extrato de tanino SM(r) com enxague apresentou resultados semelhantes aos da solução de cloro para todos os parâmetros analisados e, além disso, apresentou uma elevada redução da contagem inicial microbiológica de alface minimamente processada. No entanto, durante o armazenamento, SM(r) não apresentou melhores resultados do que o uso de água. Portanto, SM(r) poderia ser utilizado na desinfecção da água de lavagem das alfaces, reduzindo a microbiota inicial e, assim, impedir a contaminação cruzada durante o processamento mínimo de vegetais.

Mixed and monospecific stands of eucalyptus and black-wattle: I - fine root length density / Plantios monoespecíficos e mistos de eucalipto e acácia-negra: I - densidade do comprimento de raízes finas

Fine root length density (FRLD) was evaluated in mixed and monospecific stands of Eucalyptus grandis x E. urophylla and Acacia mearnsii in Southern Brazil. FRLD (≤2,0mm) at 8 and 18 months after planting in the treatments: 100E (100% of eucalyptus); 100A (100% of Acacia mearnsii); 50E:50A (50% of eucalyptus + 50% of Acacia mearnsii). The findings demonstrated that the FRLD at 8 months of age have the same distribution, in the two different species, in the distribution of the different soil layers, reaching the maximum projection of 125cm from the tree trunk. For the age of 18 months after planting, it was verified that the FRLD in the monospecific stand of Acacia mearnsii was higher than in the monoculture and mixed stand of Eucalyptus grandis x E. urophylla. Therefore, no interaction, neither positive nor negative, between the root systems of Eucalyptus grandis x E. urophylla and Acacia mearnsii during the 18 months after planting was found. The higher FRLD is found at the soil layers surface, next to the tree trunk and in the planting line, followed by the diagonal and planting rows. The initial growth in length of the root system of Acacia mearnsii is more dynamic with higher density than the eucalyptus, but without interfering directly in the global growth of fine roots in mixed stands.

Avaliou-se a densidade do comprimento de raízes finas (DCRF) de plantios monoespecíficos e misto de Eucalyptus grandis x E. urophylla e de Acacia mearnsii na região sul do Brasil. A DCRF (≤2,0mm) foi determinada aos 8 e 18 meses após o plantio nos tratamentos: 100E (100% de eucalipto); 100A (100% de Acacia mearnsii); 50E:50A (50% de eucalipto + 50% de Acacia mearnsii). A DCRF aos oito meses de idade possui o mesmo comportamento para a ocupação das diferentes camadas do solo, atingindo uma projeção máxima de 125cm de distância em relação ao tronco da árvore. Já, aos 18 meses após o plantio, verificou-se que, no cultivo monoespecífico de Acacia mearnsii, a DCRF foi superior em relação ao monocultivo e plantio misto de Eucalyptus grandis x E. urophylla. Não ocorreram interações positivas ou negativas entre os sistemas radiculares do eucalipto e da acácia-negra durante os primeiros 18 meses após o plantio. A maior DCRF encontra-se nas camadas superficiais do solo, nas proximidades do tronco da árvore e na linha de plantio, seguida pela diagonal e entrelinha de plantio. O crescimento inicial em comprimento do sistema radicular da Acacia mearnsii é mais dinâmico e maior do que a do eucalipto, mas sem interferir diretamente no crescimento global das raízes finas no cultivo misto.

Mixed and monospecific stands of eucalyptus and black-wattle: I - fine root length density

Fine root length density (FRLD) was evaluated in mixed and monospecific stands of Eucalyptus grandis x E. urophylla and Acacia mearnsii in Southern Brazil. FRLD (2,0mm) at 8 and 18 months after planting in the treatments: 100E (100% of eucalyptus); 100A (100% of Acacia mearnsii); 50E:50A (50% of eucalyptus + 50% of Acacia mearnsii). The findings demonstrated that the FRLD at 8 months of age have the same distribution, in the two different species, in the distribution of the different soil layers, reaching the maximum projection of 125cm from the tree trunk. For the age of 18 months after planting, it was verified that the FRLD in the monospecific stand of Acacia mearnsii was higher than in the monoculture and mixed stand of Eucalyptus grandis x E. urophylla. Therefore, no interaction, neither positive nor negative, between the root systems of Eucalyptus grandis x E. urophylla and Acacia mearnsii during the 18 months after planting was found. The higher FRLD is found at the soil layers surface, next to the tree trunk and in the planting line, followed by the diagonal and planting rows. The initial growth in length of the root system of Acacia mearnsii is more dynamic with higher density than the eucalyptus, but without interfering directly in the global growth of fine roots in mixed stands.

Avaliou-se a densidade do comprimento de raízes finas (DCRF) de plantios monoespecíficos e misto de Eucalyptus grandis x E. urophylla e de Acacia mearnsii na região sul do Brasil. A DCRF (2,0mm) foi determinada aos 8 e 18 meses após o plantio nos tratamentos: 100E (100% de eucalipto); 100A (100% de Acacia mearnsii); 50E:50A (50% de eucalipto + 50% de Acacia mearnsii). A DCRF aos oito meses de idade possui o mesmo comportamento para a ocupação das diferentes camadas do solo, atingindo uma projeção máxima de 125cm de distância em relação ao tronco da árvore. Já, aos 18 meses após o plantio, verificou-se que, no cultivo monoespecífico de Acacia mearnsii, a DCRF foi superior em relação ao monocultivo e plantio misto de Eucalyptus grandis x E. urophylla. Não ocorreram interações positivas ou negativas entre os sistemas radiculares do eucalipto e da acácia-negra durante os primeiros 18 meses após o plantio. A maior DCRF encontra-se nas camadas superficiais do solo, nas proximidades do tronco da árvore e na linha de plantio, seguida pela diagonal e entrelinha de plantio. O crescimento inicial em comprimento do sistema radicular da Acacia mearnsii é mais dinâmico e maior do que a do eucalipto, mas sem interferir diretamente no crescimento global das raízes finas no cultivo misto.

Mixed and monospecific stands of eucalyptus and black-wattle: I - fine root length density

Fine root length density (FRLD) was evaluated in mixed and monospecific stands of Eucalyptus grandis x E. urophylla and Acacia mearnsii in Southern Brazil. FRLD (2,0mm) at 8 and 18 months after planting in the treatments: 100E (100% of eucalyptus); 100A (100% of Acacia mearnsii); 50E:50A (50% of eucalyptus + 50% of Acacia mearnsii). The findings demonstrated that the FRLD at 8 months of age have the same distribution, in the two different species, in the distribution of the different soil layers, reaching the maximum projection of 125cm from the tree trunk. For the age of 18 months after planting, it was verified that the FRLD in the monospecific stand of Acacia mearnsii was higher than in the monoculture and mixed stand of Eucalyptus grandis x E. urophylla. Therefore, no interaction, neither positive nor negative, between the root systems of Eucalyptus grandis x E. urophylla and Acacia mearnsii during the 18 months after planting was found. The higher FRLD is found at the soil layers surface, next to the tree trunk and in the planting line, followed by the diagonal and planting rows. The initial growth in length of the root system of Acacia mearnsii is more dynamic with higher density than the eucalyptus, but without interfering directly in the global growth of fine roots in mixed stands.

Avaliou-se a densidade do comprimento de raízes finas (DCRF) de plantios monoespecíficos e misto de Eucalyptus grandis x E. urophylla e de Acacia mearnsii na região sul do Brasil. A DCRF (2,0mm) foi determinada aos 8 e 18 meses após o plantio nos tratamentos: 100E (100% de eucalipto); 100A (100% de Acacia mearnsii); 50E:50A (50% de eucalipto + 50% de Acacia mearnsii). A DCRF aos oito meses de idade possui o mesmo comportamento para a ocupação das diferentes camadas do solo, atingindo uma projeção máxima de 125cm de distância em relação ao tronco da árvore. Já, aos 18 meses após o plantio, verificou-se que, no cultivo monoespecífico de Acacia mearnsii, a DCRF foi superior em relação ao monocultivo e plantio misto de Eucalyptus grandis x E. urophylla. Não ocorreram interações positivas ou negativas entre os sistemas radiculares do eucalipto e da acácia-negra durante os primeiros 18 meses após o plantio. A maior DCRF encontra-se nas camadas superficiais do solo, nas proximidades do tronco da árvore e na linha de plantio, seguida pela diagonal e entrelinha de plantio. O crescimento inicial em comprimento do sistema radicular da Acacia mearnsii é mais dinâmico e maior do que a do eucalipto, mas sem interferir diretamente no crescimento global das raízes finas no cultivo misto.

Mixed and monospecific stands of eucalyptus and black-wattle: I - fine root length density

Fine root length density (FRLD) was evaluated in mixed and monospecific stands of Eucalyptus grandis x E. urophylla and Acacia mearnsii in Southern Brazil. FRLD (2,0mm) at 8 and 18 months after planting in the treatments: 100E (100% of eucalyptus); 100A (100% of Acacia mearnsii); 50E:50A (50% of eucalyptus + 50% of Acacia mearnsii). The findings demonstrated that the FRLD at 8 months of age have the same distribution, in the two different species, in the distribution of the different soil layers, reaching the maximum projection of 125cm from the tree trunk. For the age of 18 months after planting, it was verified that the FRLD in the monospecific stand of Acacia mearnsii was higher than in the monoculture and mixed stand of Eucalyptus grandis x E. urophylla. Therefore, no interaction, neither positive nor negative, between the root systems of Eucalyptus grandis x E. urophylla and Acacia mearnsii during the 18 months after planting was found. The higher FRLD is found at the soil layers surface, next to the tree trunk and in the planting line, followed by the diagonal and planting rows. The initial growth in length of the root system of Acacia mearnsii is more dynamic with higher density than the eucalyptus, but without interfering directly in the global growth of fine roots in mixed stands.

Avaliou-se a densidade do comprimento de raízes finas (DCRF) de plantios monoespecíficos e misto de Eucalyptus grandis x E. urophylla e de Acacia mearnsii na região sul do Brasil. A DCRF (2,0mm) foi determinada aos 8 e 18 meses após o plantio nos tratamentos: 100E (100% de eucalipto); 100A (100% de Acacia mearnsii); 50E:50A (50% de eucalipto + 50% de Acacia mearnsii). A DCRF aos oito meses de idade possui o mesmo comportamento para a ocupação das diferentes camadas do solo, atingindo uma projeção máxima de 125cm de distância em relação ao tronco da árvore. Já, aos 18 meses após o plantio, verificou-se que, no cultivo monoespecífico de Acacia mearnsii, a DCRF foi superior em relação ao monocultivo e plantio misto de Eucalyptus grandis x E. urophylla. Não ocorreram interações positivas ou negativas entre os sistemas radiculares do eucalipto e da acácia-negra durante os primeiros 18 meses após o plantio. A maior DCRF encontra-se nas camadas superficiais do solo, nas proximidades do tronco da árvore e na linha de plantio, seguida pela diagonal e entrelinha de plantio. O crescimento inicial em comprimento do sistema radicular da Acacia mearnsii é mais dinâmico e maior do que a do eucalipto, mas sem interferir diretamente no crescimento global das raízes finas no cultivo misto.

Avaliação econômica da recuperação de áreas mineradas na empresa Copelmi Mineração S.A., Butiá, RS, Brasil

On the base of an inventory three economical criteria (Internal Rate of Return, Net Present Value and Benefit Cost Ratio) were calculated to analyze the economic results of plantations of Acacia mearnsii and Pinus sp and a grass cover on degraded areas in Mina de Recreio (COPELMI Mineração S.A), in Butiá, Rio Grande do Sul, Brazil. The plantation of Acacia mearnsii with a rotation age of 5 years was found as the economically optimal variant.

Com base em um inventário, foram calculados três critérios econômicos (Taxa Interna de Retorno, Valor Presente Líquido e Relação Benefício Custo) para avaliar os resultados econômicos de plantações de acácia-negra, Pinus sp e de uma cobertura de grama para áreas degradadas na Mina de Recreio (COPELMI Mineração S.A.), em Butiá, RS. Como variante, economicamente ótima, foi encontrada a plantação com acácia-negra com uma idade de rotação de 5 anos.

Avaliação econômica da recuperação de áreas mineradas na empresa Copelmi Mineração S.A., Butiá, RS, Brasil

On the base of an inventory three economical criteria (Internal Rate of Return, Net Present Value and Benefit Cost Ratio) were calculated to analyze the economic results of plantations of Acacia mearnsii and Pinus sp and a grass cover on degraded areas in Mina de Recreio (COPELMI Mineração S.A), in Butiá, Rio Grande do Sul, Brazil. The plantation of Acacia mearnsii with a rotation age of 5 years was found as the economically optimal variant.

Com base em um inventário, foram calculados três critérios econômicos (Taxa Interna de Retorno, Valor Presente Líquido e Relação Benefício Custo) para avaliar os resultados econômicos de plantações de acácia-negra, Pinus sp e de uma cobertura de grama para áreas degradadas na Mina de Recreio (COPELMI Mineração S.A.), em Butiá, RS. Como variante, economicamente ótima, foi encontrada a plantação com acácia-negra com uma idade de rotação de 5 anos.

Ciclagem de nutrientes em Acacia mearnsii de wild. V. Quantificação do conteúdo de nutrientes na biomassa aérea de Acacia mearnsii de wild. Procedência australiana

Nutrient contents of 2.4 years old black wattle (.), from Bodalla Australian provenance, were quantified. This provenance was established on soils of low fertility and high acidity, at Menezes Farm of Agroseta S.A. Forest CompAcacia mearnsii De Wildany in the Capão Comprido District, municipality of Butiá-RS. A total of nine trees were selected to form the sample. The destructive sampling was constituted in the individualization of compartments of above-ground biomass (leaves, live branches, dead branches, bark and wood) to determine dry matter and nutrient contents. The quantity of total nutrients in the above-ground biomass from Bodalla provenance was 182.1kg ha-1 of N; 8.2kg ha-1 of P; 104.4kg ha-1 of K; 66.7kg ha-1 of Ca; 16.1kg ha-1 of Mg and 10.0Kg. ha-1 of S. For the Bodalla provenance, 57.4% of dry matter was allocated to the leaves, live branches, dead branches, bark and wood, containing 74% of N; 72.1% of P; 63% of K; 68.5% of Ca, 69.3% of Mg and 74.1% of S in the total found in the aerial part. The trunk component (bark and wood) accumulated 26% N; 27.9% P; 37% K; 31,5% Ca; 30.7% Mg and 25.8% S.

No presente trabalho foi quantificado o conteúdo de nutrientes na procedência Australiana Bodalla de Acácia-negra (Acacia mearnsii De Wild.), aos 2,4 anos de idade. A procedência encontra-se estabelecida em solo de baixa fertilidade, com acidez elevada e localizado na Fazenda Menezes, no Distrito de Capão Comprido, município de Butiá-RS, pertencente à Empresa Florestal Agroseta S.A.. Foi selecionado um total de nove árvores para comporem as amostras. A amostragem destrutiva constituiu na individualização dos compartimentos da biomassa aérea (folhas, galhos vivos, galhos mortos, casca e madeira) visando à determinação da matéria seca e do conteúdo de nutrientes. As quantidades de nutrientes contidos na biomassa aérea total da procedência Bodalla foram de 182,1kg ha-1 de N; 8,2kg ha-1 de P; 104,4kg ha-1 de K; 66,7kg ha-1 de Ca; 16,1kg ha-1 de Mg e 10,0kg ha-1 de S. Na procedência Bodalla, 57,4% da matéria seca foi alocada para folhas, galhos vivos e galhos mortos, contento 74% do N; 72,1% do P; 63% do K; 68,5% do Ca, 69,3% do Mg e 74,1% do S do total existente na parte aérea. O componente fuste ( casca e madeira) acumulou 26% do N; 27,9% do P; 37% do K; 31,5% do Ca; 30,7% do Mg e 25,8% do S.