RESUMO
Objective:To observe the effect of Radix Paeoniae Rubra on intimal proliferation,activating of angiotensin Ⅱ(AgⅡ) and expression of Mitogen-activated protein kinase phosphatase-1(MKP-1) after carotid artery balloon injury in cholesterol-fed rabbits.Methods: Male rabbits were randomly divided into Radix Paeoniae Rubra(PPR) groups: high dose group(75、50、25g.kg-1.d-1;n=8),middle dose group(2g.kg-1.d-1),low dose group(1g.kg-1.d-1;n=8) and control group.Both groups received high fat forage(2% cholesterol + 5% lard).Balloon injury of carotid artery was performed.Carotid artery were harvested at the end of 10 weeks.Expression level of AgⅡwas measured by radioimmunoassay.MKP-1 expression was determined by RT-PCR.Immunohistochemical staining and morphological detection were adopted.Results: Compare with control group,expression of AgⅡ decreased obviously(P
RESUMO
Objective To observe the relationship between fibroblast and angiotensin Ⅱ and,the influence of angiotensin Ⅱ on human renal interstitial fibroblast(HRIF).Methods Separated and cultured renal interstitial fibroblasts were identified and the 3H-TDR incorporation,flow cytometry and immunohistochemical technique were applied to observe the effect of angiotensin Ⅱ on the cell cycle and DNA synthesis of HRIF and the inhibitive effect of Losartan.Results Angiotensin Ⅱ (10 -10M-10 -6M) had elevated the incorporation rate of HRIF 3H-TDR and had showed dose-dependence in day 1 and day 3(? 1=0.709,P
