Dichoptic Rescue for Spectacle-Flip Sabotage of Anisometropic Amblyopia Therapy.

Background: To be successful, amblyopia therapeutic techniques must respond when ingenious (shrewd) amblyopic children sabotage treatment effect by discovering ways(1) to alter those therapy aspects they find frustrating. Methods: Her guardian consented to IRB-approved study outside typical FDA enrollment guidelines for CureSight dichoptic therapy. Acuity (EVA) and stereopsis was by PEDIG protocols. Results: A 6-year-old rural Alaska native girl in foster care had previously been diagnosed with anisometropic amblyopia and treated with spectacles and patching but had poor compliance with BCVA 0.9-1.0 logMAR. She demonstrated exceptional creativity and hyperactivity but had no other medical issues. Atropine was prescribed in addition to her spectacles +5.50+1.00x90 and +0.50+1.00x90, but the patient discovered the rare "Spectacle Flip" method that sabotaged therapeutic impact. She had EVA enrollment acuities of logMAR 0.9 right and -0.1 left with stereo 400 seconds of arc. After just one month dichoptic therapy 1.5 hours 6 days per week, acuity improved to logMAR 0.7 and -0.1 with stereo up to 140 second of arc. Four months later acuity was 0.6 right and -0.2 left. Stereoacuity improved from 400 to 200 arc seconds. Conclusion: Even when conventional compliance methods fail, dichoptic therapy with eye-tracking has the novel and unique potential to improve amblyopia treatment compliance with real-time monitored fixation compliance and confirmation of correct spectacle wear. Clinical Trial Registration: NCT06165705.

Dichoptic therapy with eye-tracking improved compliance and achieved de novo acuity and stereo gains in a girl with severe refractory anisometropic amblyopia compounded by the rare complication of topical atropine therapy called "spectacle-flip sabotage".

A pilot non-randomized trial of smartphone-based anaglyph system for treatment of adult amblyopia through VR (Virtual Reality).

Purpose: To evaluate the effectiveness of amblyopia treatment through a smartphone-based anaglyph system by virtual reality (VR) in adult patients. Methods: A total of 10 subjects diagnosed with anisometropic amblyopia were enrolled during the study period. Best Corrected Visual Acuity (BCVA), stereoacuity, and contrast acuity were evaluated during three visits (at presentation, 3 months and 6 months) of smartphone-based anaglyph video run in the VR mode. All the amblyogenic factors including stereopsis, color vision, and contrast acuity were compared using Friedman two-way analysis of variance. Statistical significance was considered if P < 0.05. Results: Mean BCVA in amblyopic eye improved significantly from a logMAR value of 0.73 ± 0.64 before Virtual reality vision therapy (VRVT) to a post-training VRVT value of 0.48 ± 0.44 (P < 0.01). Mean stereoacuity changed from a value of 560.00 ± 301.58 before VRVT to a value of 263.00 ± 143.58 seconds of arc after training (VRVT) (P < 0.01). Mean accommodation changes from a value of 15.00 ± 7.40 before training or VRVT to value of 12.60 ± 6.10 cm after training (P < 0.01). Mean contrast acuity changes from a value of 1.21 ± 0.72 at presentation to a value of 1.52 ± 0.49 log unit after VRVT. Conclusion: A smartphone-based anaglyph system using VR vision therapy appears to be an effective treatment option for amblyopia in adults.

Perimortem Skeletal Sharp Force Trauma: Detection Reliability on CT Data, Demographics and Anatomical Patterns from a Forensic Dataset.

The increasing importance of trauma analysis by means of postmortem computed tomography (PMCT) is insufficiently reflected in forensic curricula, nor are best practice manuals available. We attempt to detect sharp force bone lesions on PMCT of closed forensic cases with the aims of assessing errors and pointing out patterns in anatomical location and manner of death (MOD). We investigated 41 closed sharp force fatality cases, with available PMCT and forensic reports. Two observers with different radiological training assessed the lesions on PMCT scans (2D and 3D) for comparison with the reports. Between 3% (suicides) and 15.3% (homicides) of sharp force injuries caused visible bone lesions. While our observations were repeatable, each forensic investigation left a similar number of bone lesions undetected. Injury patterns differed between MOD, with thoracic bone lesions being most frequent overall. Soft tissue injury location varied between the MOD. Associations between MOD and age as well as number of injuries were significant. The detection of bone lesions on PMCT for untrained forensic specialists is challenging, curricula and pertinent manuals are desirable. With the low frequency of bone lesions compared to soft tissue injuries, we should be aware when analyzing decomposed bodies.

[Creation of a Stereo-paired Bone Anatomical Chart Using Human Bone Specimens for X-ray Anatomical Education Part III: Addition of Anaglyph Three-dimensional Images for Those Who Hardly See Stereo-paired Photographs with the Naked Eye].

We published a report entitled "Creation of a stereo-paired bone anatomical chart using human bone specimen for radiation education" in this journal in order to accurately understand the surface structure and three-dimensional structure of bones, and assist in bone image interpretation. However, some people cannot see stereoscopically with the naked eye. Therefore, we created anaglyph three-dimensional (3D) images from stereo-paired images of the stereo X-ray anatomical chart of the bone specimen. The anaglyph of the bone surface and X-ray images facilitates stereoscopic viewing with red-blue 3D glasses. The stereo X-ray anatomical chart of the bone specimen with anaglyph 3D images was converted into an electronic data file in the same manner as the stereo X-ray anatomical chart of the bone specimen, which can be easily used in any radiological examination rooms or at home through an electronic medium. We made it possible to perform correlative stereoscopic observations of the bone surface and X-ray images using red-blue 3D glasses.

Stereogram of the Living Heart, Lung, and Adjacent Structures.

Innovations in invasive cardiovascular diagnostics and therapeutics, not only limited to transcatheter approaches but also involving surgical approaches, are based on a precise appreciation of the three-dimensional living heart anatomy. Rapid advancements in three-dimensional cardiovascular imaging technologies in the 21st century have supported such innovations through the periprocedural assessment of the clinical anatomy of the living heart. However, even if high-resolution volume-rendered images are reconstructed, they cannot provide appropriate depth perception when displayed and shared on a two-dimensional display, which is widely used in clinical settings. Currently, images reconstructed from clinical datasets can visualize fine details of the cardiovascular anatomy. Therefore, this is an optimal time for cardiologists and cardiac surgeons to revisit the classic technology, stereopsis, and obtain bonus information from carefully reconstructed clinical images. Using anaglyphs or cross/uncross-fusion of paired images, striking depth perception can be readily obtained without the need for expensive equipment. This conventional technique, when applied to high-resolution volume-rendered images, may help in obtaining appropriate diagnostics, choosing optimal therapeutics, securing procedural success, and preventing complications. Furthermore, it can be used for anatomical education. In this review, we demonstrate multiple stereoscopic images reconstructed from cardiac computed tomographic datasets and discuss their clinical and educational implications.

Anaglyph stereo virtual dissection: a novel inexpensive method for stereoscopic visualisation of intracardiac anatomy on CT angiogram.

Three-dimensional visualisation is invaluable for evaluating cardiac anatomy. Patient-specific three-dimensional printed models of the heart are useful but require significant infrastructure. The three-dimensional virtual models, derived from 3D echocardiography, computed tomographic (CT) angiography or cardiac magnetic resonance (CMR), permit excellent visualisation of intracardiac anatomy, but viewing on a two-dimensional screen obscures the third dimension. Various forms of extended reality, such as virtual reality and augmented reality, augment the third dimension but only using expensive equipment. Herein, we report a simple technique of anaglyph stereoscopic visualisation of three-dimensional virtual cardiac models. The feasibility of achieving stereovision on a personal computer, using open-source software, and the need for inexpensive anaglyph glasses for viewing make it extremely cost-effective. Further, the retained depth perception of resulting stereo images in electronic and printed format makes sharing with other members of the team easy and effective.

Evaluation of stereoacuity with a digital mobile application.

PURPOSE: Stereopsis is a fundamental skill in human vision and visual actions. There are many ways to test and quantify stereoacuity: traditional paper and new digital applications are both valid ways to test the stereoacuity. The aim of this study is to compare the results obtained using standard tests and the new Stereoacuity Test App developed by the University of Bergamo. METHODS: A group of 497 children (272 males), aged between 6 and 11 years old, were tested using different tests for the quantification of stereopsis at near. These tests were TNO, Weiss EKW, and the new developed Stereoacuity Test App. RESULTS: A one-way repeated measure ANOVA showed that the three tests give different thresholds of stereoacuity (p < 0.0001). Post hoc analyses with Bonferroni correction showed that all tests showed different thresholds (p < 0.0001). The lower threshold was obtained by Titmus Stereo Test followed by Stereoacuity App, Weiss MKW, and TNO. CONCLUSION: The stereoacuity based on global stereopsis showed that the better values were obtained in order by Stereoacuity Test App, TNO, and Weiss EKW. However, the clinical significance of their values is similar. The new digital test showed a greater compliance by the child, showing itself in tune with the digital characteristics of today's children.

On the Origins of Terms in Binocular Vision.

Vision with two eyes has been commented upon for many centuries, and the principal concern has been with binocular single vision. The terminology we apply to binocular vision developed rapidly after the invention of the stereoscope in the early 19th century. The origins of terms such as anaglyph, binocular lustre, chromatic stereoscope, cyclopean eye, dichoptic, horopter, pseudoscope, rivalry, stereoscope, stereograph, and stereopsis are described together with portraits of those who introduced them.

How effective are 3D anaglyph stimuli? An analysis in emotional recognition / Quão eficazes são os estímulos de anáglifos 3D? Uma análise no reconhecimento emocional / ¿Qué eficacia tienen los estímulos anaglifos 3D? Un análisis del reconocimiento emocional

The aim of this study was to examine the role of virtual environments in visual recognition. A classic and economically accessible technique was chosen: anaglyph. A battery of images that differed in their emotional charge was developed. The emotional valence of the new stimulus was evaluated using the SAM battery. 2D and anaglyph modalities were also tested. 32 subjects participated in the first study and 42 subjects in the recognition task. The results: i) Participants were more accurate in classic 2D environments compared to 3D, ii) participants were more efficient in the negative 3D condition compared to the rest of the emotional conditions in this category, while the neutral environment was the most efficient for 2D. It is suggested that coding information would be simpler for a 2D stimulus, however, effects as robust as emotional charge would manifest more clearly in a deeper presentation (AU).

O objetivo deste estudo foi examinar o papel dos ambientes virtuais no reconhecimento visual. Foi escolhida uma técnica clássica e economicamente acessível a todos: o anáglifo. Foi desenvolvida uma bateria de imagens que diferiam em sua carga emocional. Avaliou-se a valência emocional do novo estímulo por meio da bateria SAM. As modalidades 2D e anáglifo também foram testadas. Participaram 32 sujeitos no primeiro estudo e 42 sujeitos na tarefa de reconhecimento. Os resultados: i) Os participantes foram mais precisos nos ambientes 2D clássicos em comparação com os 3D; ii) os participantes foram mais eficientes na condição negativa 3D em comparação com o resto das condições emocionais nessa categoria, enquanto o ambiente neutro foi o mais eficiente para 2D. Sugere-se que a codificação da informação seria mais simples para um estímulo 2D, no entanto, efeitos tão robustos quanto a carga emocional se manifestariam mais claramente em uma apresentação mais profunda (AU).

El objetivo de este estudio fue examinar el papel de los entornos virtuales en el reconocimiento visual. Se eligió una técnica clásica y económicamente accesible: el anaglifo. Se desarrolló una batería de imágenes que diferían en su carga emocional. La valencia emocional del nuevo estímulo fue evaluada a través de la batería SAM. Se probaron las modalidades 2D y anaglifo. 32 sujetos participaron en el primer estudio y 42 sujetos en la tarea de reconocimiento. Los resultados: i) los participantes fueron más precisos en entornos 2D clásicos en comparación con 3D, ii) los participantes fueron más eficientes en la condición 3D negativa en comparación con el resto de las condiciones emocionales en esta categoría, mientras que el entorno neutral fue el más eficiente para 2D. Se sugiere que la codificación de información sería más simple para un estímulo 2D, sin embargo, efectos tan robustos como la carga emocional se manifestarían más claramente en una presentación de mayor profundidad (AU).

Stereoscopy in Dental Education: An Investigation.

The aim of this study was to investigate whether stereoscopy can play a meaningful role in dental education. The study used an anaglyph technique in which two images were presented separately to the left and right eyes (using red/cyan filters), which, combined in the brain, give enhanced depth perception. A positional judgment task was performed to assess whether the use of stereoscopy would enhance depth perception among dental students at Osaka University in Japan. Subsequently, the optimum angle was evaluated to obtain maximum ability to discriminate among complex anatomical structures. Finally, students completed a questionnaire on a range of matters concerning their experience with stereoscopic images including their views on using stereoscopy in their future careers. The results showed that the students who used stereoscopy were better able than students who did not to appreciate spatial relationships between structures when judging relative positions. The maximum ability to discriminate among complex anatomical structures was between 2 and 6 degrees. The students' overall experience with the technique was positive, and although most did not have a clear vision for stereoscopy in their own practice, they did recognize its merits for education. These results suggest that using stereoscopic images in dental education can be quite valuable as stereoscopy greatly helped these students' understanding of the spatial relationships in complex anatomical structures.

Ultrastructural analysis of apatite-degrading capability of extended invasive podosomes in resorbing osteoclasts.

Osteoclasts in culture are non-transformed cell types that spontaneously develop specific cell-adhesion devices such as podosomes. An individual podosome is a complex network of filamentous actin (F-actin) unit structure that collectively, with other proteins, self-organizes as the sealing zone. Major matrix degradation on apatite seems to proceed under the ruffled-border domain, which is an enclosed extracellular compartment tightly sealed off by this sealing zone. Presently we found that usually the top of finger-like projections of the ruffled border reached toward the plane of the apatite surface, where a shallow degradation of apatite took place. Simultaneously, we obtained several pieces of structural evidence indicating that a specific protrusion referred to as an invasive podosome (invadopodium), which was continuous with podosomes derived from the sealing zone, invaded deeply into apatite matrix and degraded it. The F-actin architecture of the invasive podosome - an active extracellular matrix-degrading, actin-rich cell protrusion - could be distinguished from that of other punctate F-actin structures including the individual podosome, sealing zone, and ruffled border projection. Invasive podosomes contained 2 different F-actin populations, i.e., an interconnected meshwork and a parallel array of bundles. The morphological variability of these protrusions was apparent, having a single cylindrical to lamella-shaped cytoskeletal organization. Our present observations strongly suggest that the degradation of apatite substrate-resorbing osteoclasts appears to have been preceded by the combined appearance of ruffled border and invasive podosomes, and also occurred simultaneously with cell migration during an alternating cycle of resorption and migration.

Stereoscopic three-dimensional visualization applied to multimodal brain images: clinical applications and a functional connectivity atlas.

Effective visualization is central to the exploration and comprehension of brain imaging data. While MRI data are acquired in three-dimensional space, the methods for visualizing such data have rarely taken advantage of three-dimensional stereoscopic technologies. We present here results of stereoscopic visualization of clinical data, as well as an atlas of whole-brain functional connectivity. In comparison with traditional 3D rendering techniques, we demonstrate the utility of stereoscopic visualizations to provide an intuitive description of the exact location and the relative sizes of various brain landmarks, structures and lesions. In the case of resting state fMRI, stereoscopic 3D visualization facilitated comprehension of the anatomical position of complex large-scale functional connectivity patterns. Overall, stereoscopic visualization improves the intuitive visual comprehension of image contents, and brings increased dimensionality to visualization of traditional MRI data, as well as patterns of functional connectivity.

Anaglyph of retinal stem cells and developing axons: selective volume enhancement in microscopy images.

Retinal stem cell culture has become a powerful research tool, but it requires reliable methods to obtain high-quality images of living and fixed cells. This study describes a procedure for using phase contrast microscopy to obtain three-dimensional (3-D) images for the study of living cells by photographing a living cell in a culture dish from bottom to top, as well as a procedure to increase the quality of scanning electron micrographs and laser confocal images. The procedure may also be used to photograph clusters of neural stem cells, and retinal explants with vigorous axonal growth. In the case of scanning electron microscopy and laser confocal images, a Gaussian procedure is applied to the original images. The methodology allows for the creation of anaglyphs and video reconstructions, and provides high-quality images for characterizing living cells or tissues, fixed cells or tissues, or organs observed with scanning electron and laser confocal microscopy. Its greatest advantage is that it is easy to obtain good results without expensive equipment. The procedure is fast, precise, simple, and offers a strategic tool for obtaining 3-D reconstructions of cells and axons suitable for easily determining the orientation and polarity of a specimen. It also enables video reconstructions to be created, even of specimens parallel to the plastic base of a tissue culture dish, It is also helpful for studying the distribution and organization of living cells in a culture, as it provides the same powerful information as optical tomography, which most confocal microscopes cannot do on sterile living cells.

[Three-dimensional endoscopic endonasal study of skull base anatomy]. / Estudio endoscópico endonasal 3D de la anatomía de la base de cráneo.

INTRODUCTION: Training in dissection of the paranasal sinuses and the skull base is essential for anatomical understanding and correct surgical techniques. Three-dimensional (3D) visualisation of endoscopic skull base anatomy increases spatial orientation and allows depth perception. OBJECTIVE: To show endoscopic skull base anatomy based on the 3D technique. METHODS: We performed endoscopic dissection in cadaveric specimens fixed with formalin and with the Thiel technique, both prepared using intravascular injection of coloured material. Endonasal approaches were performed with conventional 2D endoscopes. Then we applied the 3D anaglyph technique to illustrate the pictures in 3D. RESULTS: The most important anatomical structures and landmarks of the sellar region under endonasal endoscopic vision are illustrated in 3D images. CONCLUSION: The skull base consists of complex bony and neurovascular structures. Experience with cadaver dissection is essential to understand complex anatomy and develop surgical skills. A 3D view constitutes a useful tool for understanding skull base anatomy.

Reconstruction of Stereo MR Angiography Optimized to View Position and Distance using MIP

PURPOSE: We studied enhanced method to view the vessels in the brain using Magnetic Resonance Angiography (MRA). Noticing that Maximum Intensity Projection (MIP) image is often used to evaluate the arteries of the neck and brain, we propose a new method for view brain vessels to stereo image in 3D space with more superior and more correct compared with conventional method. MATERIALS AND METHODS: We use 3T Siemens Tim Trio MRI scanner with 4 channel head coil and get a 3D MRA brain data by fixing volunteers head and radiating Phase Contrast pulse sequence. MRA brain data is 3D rotated according to the view angle of each eyes. Optimal view angle (projection angle) is determined by the distance between eye and center of the data. Newly acquired MRA data are projected along with the projection line and display only the highest values. Each left and right view MIP image is integrated through anaglyph imaging method and optimal stereoscopic MIP image is acquired. RESULTS: Result image shows that proposed method let enable to view MIP image at any direction of MRA data that is impossible to the conventional method. Moreover, considering disparity and distance from viewer to center of MRA data at spherical coordinates, we can get more realistic stereo image. In conclusion, we can get optimal stereoscopic images according to the position that viewers want to see and distance between viewer and MRA data. CONCLUSION: Proposed method overcome problems of conventional method that shows only specific projected image (z-axis projection) and give optimal depth information by converting mono MIP image to stereoscopic image considering viewers position. And can display any view of MRA data at spherical coordinates. If the optimization algorithm and parallel processing is applied, it may give useful medical information for diagnosis and treatment planning in real-time.