RESUMO
Cultured T.vaginalis was used for the anti-trichomonas test at different times,concentrations of propolis and densities of the parasites.After being cultured for 0,6,12,24 hrs,the survival rate of the parasites was(91.50?3.11)%,(43.00?6.83)%,(22.25?5.32)% and(11.50?5.74)% respectively with a significant difference between propolis group and the control.Under the concentrations of 0.24,0.48,0.96,1.92,3.84 and 7.68(mg/ml) with 24 hrs culture,the survival rate was(88.00?5.29)%,(92.67?4.16)%,(90.0?6.00)%,(84.00?4.00)%,(2.67?1.15)%,and 0 respectively.The results showed that propolis possesses clear in vitro anti-trichomonas activity which is relevant to the duration of culture and the concentration of the agent.
RESUMO
Objective To research the trichomonacidal effect of secnidazole benzoate in vitro.Methods Trichomonas vaginalis was cultured in liver extract medium in 96-well microplate.The culture suspension of Trichomonas vaginalis was divided into four groups:secnidazole benzoate, secnidazole, metronidazole and control, with medium as blank control.MTT colorimetric assay was applied to determine the inhibitory effect of secnidazole benzoate on the proliferation of Trichomonas vaginalis.The culture suspension was transfered into test tubes and divided into same groups to observe inhibitory effect by the classical microscopic counting method.Results After 24 h incubation, the proliferation of the parasites was concentration-dependent by secnidazole benzoate(t=9.02, P
