Research progress in the clinical application of autoantibodies in juvenile idiopathic arthritis / 中华实用儿科临床杂志

Juvenile idiopathic arthritis is a common connective tissue disease of children.The rate of disability is high.Therefore,early diagnosis is important.There are some study on the value of antibodies in juvenile idiopathic arthritts,such as rheumatoid factor,antiperinuclear factor,antikeratin antibody,antibodies to cyclic citrullinated peptides and so on.This review introduces the progress of them.

Age-related diagnostic utility of rheumatoid factor, anticyclic citrullinated peptide and antikeratin antibodies in Chinese patients with rheumatoid arthritis.

OBJECTIVES: Retrospective study to evaluate the diagnostic utility of rheumatoid factor (RF), anticyclic citrullinated peptide antibodies (ACPA) and antikeratin antibodies (AKA) in a broad age range of patients with rheumatoid arthritis (RA). METHODS: Clinical and serological data from patients with RA were collected and analysed. Patients were stratified according to age (<16 years [juvenile idiopathic arthritis; JIA], 16-40 years; 41-60 years and >60 years) and sex. RESULTS: The study included 3725 patients. There were no significant sex-related differences in rates of RF, ACPA or AKA positivity. RF, ACPA and AKA positivity were significantly less common in patients aged <16 years than those aged ≥ 16 years. There were no other significant differences between age groups. CONCLUSIONS: RF, ACPA and AKA have better diagnostic value for RA in adult patients than in patients with JIA. A combination of RF, ACPA and AKA serological testing may be a useful diagnostic tool for RA in Chinese adults.

Nuevos marcadores en el diagnóstico de la artritis reumatoide / New markers in the diagnosis of rheumatoid arthritis

Se hizo una revisión sobre otros marcadores de diagnóstico para la artritis reumatoidea, una de las enfermedades autoinmunes sistémicas más comunes y de amplia distribución mundial, que afecta principalmente a las mujeres y es muy agresiva en la raza negra. Su diagnóstico precoz resulta de vital importancia para evitar las deformidades articulares características y para mejorar la calidad de vida de los pacientes. Actualmente se diagnostica siguiendo los criterios de clasificación definidos por la American Rheumatism Association, entre ellos, la detección del factor reumatoideo. Hoy día se sabe que este no es patognomónico de la enfermedad porque puede estar presente en otras entidades. Se realizó una revisión de otros marcadores sexológicos que están asociados a la artritis reumatoidea y que son mucho más específicos, de manera que podría considerarse su uso en el diagnóstico precoz de esta enfermedad.

A review of other diagnostic markers for rheumatoid arthritis, one of the commonest autoimmune systemic diseases, was made. It is widely spread all over the world. It mainly affects women and it is very aggressive among black people. Its early diagnosis is of vital importance to prevent the characteristic joint deformities and to improve the patients’ quality of life. At present, it is diagnosed according to the classification criteria defined by the American Rheumatism Association, among them, the detection of the rheumatoid factor. It is known that it is not pathognomonic of the disease, since it may be present in other entities. A review of other sexological markers that are associated with rheumatoid arthritis and that are much more specific was made, so that their use could be considered in the early diagnosis of this disease,.

Significance of antibodies to filaggrin in diagnosis of rheumatoid arthritis / 基础医学与临床

Objective To determine the diagnostic value of antifilaggrin antibodies(AFA) in rheumatoid arthritis(RA) and to compare the correlation among AFA,anti-perinuclear factor(APF) and anti-keratin antibodies(AKA). Methods Filaggrin was extracted from human epidermis and then partially purified. AFA in 103 cases of RA and 140 cases of controls were detected by western blot. APF and AKA were detected using indirect immunofluorescence. Results AFA test resulted in diagnosis of 35.9% of 103 RA samples, with a specificity of 93.7%. The result was significantly higher than the controls (P

The diagnostic value of anti-cyclic citrullinated peptide antibody in patients with rheumatoid arthritis / 中华风湿病学杂志

Objective To explore the diagnostic value of anti cyclic citrullinated peptide (CCP) antibody (anti CCP) detected by ELISA in patients with rheumatoid arthritis (RA).Methods The synthesized CCP deduced from the known cDNA sequence was used as substrate in ELISA.The anti CCP was detected by ELISA in 294 RA,132 rheumatic diseases other than RA and 135 non rheumatic diseases.To compare the correlation among the anti CCP and APF (antiperinuclear factor),AKA (anti keratin antibody),RF (rheumatoid factor) and HLA DR4.Results One hundred and thirty seven (46 6%) RA sera,7 (5 3%) other rheumatic disease sera and 2 (1 8%) non rheumatic disease were found to be anti CCP positive by ELISA.The sensitivity of anti CCP was 46 6% with high specificity (96 9%) in RA.Anti CCP was associated with APF,AKA and HLA DR4 except RF.Conclusions Anti CCP presents a reasonable sensitivity (46 6%) with high specificity (96 6%) to RA and it is valuable to RA diagnosis.Anti CCP is associated with APF,AKA and HLA DR4 but not completely overlaps them.Anti CCP could be regarded as a new diagnostic marker in RA.

Clinical Significance of Antikeratin Antibody in Korean Patients with Rheumatoid Arthritis / 대한류마티스학회지

OBJECTIVES: Antikeratin antibody is an autoantibody to the cornified epithelium of rat esophagus and known to be a specific marker in Caucasian patients with rheumatoid arthritis. This study was performed to evaluate the prevalence and clinical correlation of the antibody in Korean patients with rheumatoid arthritis. METHOD: We measured antikeratin antibodies by indirect immunofluorescence method and evaluated functional class, anatomical stage, grip strength, Ritchie articular index, tender joint count, swollen joint count, rheumatoid factor and antinuclear antibody in 88 patients with rheumatoid arthritis, 20 patients with systemic lupus erythematosus and 43 healthy controls. RESULTS: The sevsitivity and specificity of the antikeratin antibody test was 48% and 98% respectively in Korean patients with rheumatoid arthritis. Intensity of antikeratin antibody was correlated with duration of morning stiffness(r=0.22, p=0.01) and rheumatoid factor titer(r=0.23, p=0.04). Antikeratin antibody was positive in 8(31%) out of 26 patients without rheumatoid factor. CONCLUSION: Antikeratin antibody is a specific marker in Korean patients with rheumatoid arthritis and correlated with duration of morning stiffness and rheumatoid factor titer.