Rat Experimental Autoimmune Gastritis Model.

BACKGROUND: Autoimmune gastritis (AIG) is an autoimmune disease of the stomach characterized by the destruction of the oxyntic mucosa, which stops producing acid and becomes both functionally and morphologically atrophic. The pathogenic mechanisms behind the disease are still poorly understood. There is no early diagnosis and specific AIG therapy. To elucidate the pathogenesis of AIG, to search for early diagnostic markers, as well as to test new therapeutic approaches, an adequate and easily reproducible experimental model for autoimmune gastritis (EAG) is needed. Existing EAG models have some limitations, including slow development of signs, absence of advanced gastritis, irrational use of animals to obtain antigen. The aim was to find out whether it is possible to cause autoimmune gastritis similar to human disease in Wistar rats through immunization with a homologous gastric mucosa extract. METHODS: Wistar rats were immunized with gastric mucosa extract. Histology studies and evaluation of serological parameters were performed 56 and 91 days later. RESULTS: Destruction of oxyntic glands by infiltrating T lymphocytes were detected in rats on 56 and 91 days after initial immunization with gastric mucosa extract. Hyperplasia of enterochromaffin-like (ECL) cells was detected on the 91st day. Antral mucosa remained unchanged. CONCLUSION: Wistar rats, immunized with gastric mucosa extract, developed EAG similar to human AIG. The advantages of received EAG model are the ease of obtaining, the rapid development of oxyntic mucosa damage, which may progress to ECL cell hyperplasia.

Prolapsed Antral Mucosa: Variant of Maxillary Sinus Mucocele / 대한이비인후과학회지

BACKGROUND AND OBJECTIVES: An array of diseases may cause masses in the maxillary sinus. Among the various diseases, the prolapsed antral mucosa (PAM) presents an inflammed, swollen maxillary antral mucosa which becomes redundant and prolapsed into the nasal cavity through a widened natural ostium, mimicking an antrochoanal polyp. The purposes of this study were to introduce prolapsed antral mucosa and evaluate the differences between prolapsed antral mucosa and other diseases, particularly maxillary sinus mucocele. MATERIALS AND METHODS: We retrospectively analyzed 18 patients who were diagnosed with prolapsed antral mucosa at the Kangbuk Samsung Hospital between 1990 and 1998. The presenting signs and symptoms, endoscopic finding, radiological finding, histopathological finding were reviewed. RESULTS: Of the symptoms reported by patients in this study, nasal obstruction was the most frequent, followed by rhinorrhea, postnasal drip, foul odor and headache. Endoscopic findings showed protruding maxitlary antral mucosa into the middle meatus. On the CT, the prolapsed antral mucosa presented features such similar to antrochoanal polyp as unilateral and homogeneous soft tissue density which extended into the middle meatus. MR imaging was useful for differentiating the prolapsed antral mucosa from neoplasms and antrochoanal polyp. Prolapsed antral mucosa and maxilla sinus mucocele showed the same histopathological features such as epithelial lining of columnar or metaplastic squamous cells with varying glandular structures, and dense fibrous stroma intiltrated by infiammatory cells. CONCLUSIONS: The prolapsed antral mucosa is considered as a variant of maxillary sinus mucocele. History, physical examination, biopsy, CT and MM are useful for differentiating the prolapsed antral mucosa from other diseases. Although a prolapsed antral mucosa is rare, it should be considered in the diagnosis and the treatment of unilateral maxillary sinus lesion.

IMMUNOHISTOCHEMICAL STUDIES OF G-CELL AND D-CELL CHANGES IN THE ANTRAL MUCOSA OF RATS DURING EXPERIMENTAL GASTRIC ULCER / 解剖学报

53 male Wistar rats were divided into three groups, i.e. experimental ulcer group, saline control group and normal control group. The antral tissues were prepared for immunohistochemical staining at 4th, 10th, 14th, 21st and 28th days after operation. Sternberger's PAP method was used to demonstrate the gastrin cells (G cells) and somatostatin cells (D cells)in the antral mucosa in order to observe their changes during experimental gastric ulcer. The morphological relationships of G cells and D cells were examined by simultaneous double immunostaining method.The results indicated that the G cells count as well as D cells count in the antral mucosa was increased (P