Curativo de hidrocoloide em lesões por arrastamento na região distal dos membros pélvicos em cães / Hydrocolloid membrane dressing in shearing injuries in the distal part of the pelvic limbs in dogs

Background: Shearing wounds on the limbs of dogs usually affect the regions distally from the radiocarpal joint at thethoracic limb and from the tibiotarsal joint at the pelvic limb. The tissue coverage and re-epithelialization of the injuredregion are important factors that should be considered in the definitive surgical treatment. The hydrocolloid membranepromotes selective autolytic debridement and accelerates the formation of granulation tissue and epithelialization. Thepresent study aims to describe the treatment with hydrocolloid membrane dressing of three cases of shearing wounds withconcomitant orthopedic injuries in the pelvic limbs of dogs.Case: Three dogs with pelvic limb injuries after vehicular trauma were selected for treatment. Two patients had shearinglesions on the medial aspect of the pelvic limb with exposure of the tibia and fibula, the talus and the tibiotarsal joint,associated with bone loss on the medial surface of the limb and rupture of the medial collateral ligament of the tarsus.Another patient had a shearing wound on the dorsal surface of the distal region of the pelvic limb, with injury of the digitalextensor tendons and bone exposure of the second and third metatarsals. Initially, the surgical debridement of the lesionwas performed and during the first five days after trauma the wound was cleansed with chlorhexidine solution and topicalapplication of crystallized sugar daily. In this initial period a dry adherent dressing was used on the lesions, without bandagesfor immobilization of the pelvic limb. In all dogs, the hydrocolloid membrane was applied from the sixth day after initialwound management. Immediately after the application of the hydrocolloid membrane, temporary immobilization of theaffected pelvic limb with a padded Robert Jones bandage was performed. The first changes of the hydrocolloid membraneswere performed after five days of their use. Subsequently, the membranes changes...(AU)

Curativo de hidrocoloide em lesões por arrastamento na região distal dos membros pélvicos em cães / Hydrocolloid membrane dressing in shearing injuries in the distal part of the pelvic limbs in dogs

Background: Shearing wounds on the limbs of dogs usually affect the regions distally from the radiocarpal joint at thethoracic limb and from the tibiotarsal joint at the pelvic limb. The tissue coverage and re-epithelialization of the injuredregion are important factors that should be considered in the definitive surgical treatment. The hydrocolloid membranepromotes selective autolytic debridement and accelerates the formation of granulation tissue and epithelialization. Thepresent study aims to describe the treatment with hydrocolloid membrane dressing of three cases of shearing wounds withconcomitant orthopedic injuries in the pelvic limbs of dogs.Case: Three dogs with pelvic limb injuries after vehicular trauma were selected for treatment. Two patients had shearinglesions on the medial aspect of the pelvic limb with exposure of the tibia and fibula, the talus and the tibiotarsal joint,associated with bone loss on the medial surface of the limb and rupture of the medial collateral ligament of the tarsus.Another patient had a shearing wound on the dorsal surface of the distal region of the pelvic limb, with injury of the digitalextensor tendons and bone exposure of the second and third metatarsals. Initially, the surgical debridement of the lesionwas performed and during the first five days after trauma the wound was cleansed with chlorhexidine solution and topicalapplication of crystallized sugar daily. In this initial period a dry adherent dressing was used on the lesions, without bandagesfor immobilization of the pelvic limb. In all dogs, the hydrocolloid membrane was applied from the sixth day after initialwound management. Immediately after the application of the hydrocolloid membrane, temporary immobilization of theaffected pelvic limb with a padded Robert Jones bandage was performed. The first changes of the hydrocolloid membraneswere performed after five days of their use. Subsequently, the membranes changes...

Evaluación de los parámetros de desempeño de un contador hematológico / Evaluation of performance parameters of an automated hematology analyzer / Avaliação dos parâmetros de desempenho de um analisador da hematologia

El primer paso para obtener seguridad en los resultados emitidos por el laboratorio clínico es confirmar que los procedimientos de medida utilizados tienen un desempeño analítico aceptable. Para conseguirlo se verificó el contador hematológico Beckman Coulter LH 750 de acuerdo con las especificaciones del fabricante y con los requisitos de calidad de este laboratorio. Los parámetros de desempeño evaluados, tanto en modo manual como automático, fueron: porcentaje de arrastre, según protocolo CLSI H26-A2, precisión en condiciones de repetibilidad, precisión en condiciones de precisión intermedia y veracidad, según protocolo CLSI EP15-A2 utilizando controles BIO-RAD, intervalo de medición, según protocolo CLSI EP6-A, límite de cuantificación, según protocolo CLSI EP17-A2 e intervalos de referencia, según protocolo CLSI EP28-A3C. Los datos se analizaron mediante LinChecker y GraphPad 5. En los ensayos realizados se cumplió con las especificaciones estipuladas por el fabricante, como así también con el requisito de calidad de este laboratorio que es variabilidad biológica mínima. También se verificaron los intervalos de referencia para individuos adultos. De esta manera, se logró realizar la verificación del contador hematológico, evidenciando que los parámetros analíticos evaluados tienen un desempeño aceptable.

The first step for safety in the results issued by the clinical laboratory is to confirm that all analytical measurement procedures have shown an acceptable analytical performance. A quality performance evaluation of automated hematology analyzer Beckman Coulter LH 750 was performed according to the quality requirements of our laboratory and manufacturer's specifications. The performance parameters evaluated by both manual and automatic mode were: carryover according to CLSI H26-A2 protocol; repeatability, intermediate precision and trueness according to CLSI EP15-A2 protocol and using BIO-RAD controls; linearity according to CLSI EP6-A protocol; quantification limits according to CLSI EP17-A2 protocol; and reference intervals according to CLSI EP28-A3C protocol. Data were analyzed using LinChecker and GraphPad5 programs. The tests performed complied with the requirements stipulated by the manufacturer and the quality requirements of our laboratory like minimal biological variability. Reference intervals for adult individuals were also checked. Consequently, performance evaluation of the automated hematology analyzer showed that the assessed laboratory parameters have acceptable performance.

O primeiro passo para obter segurança nos resultados emitidos pelo laboratório clínico é confirmar que os processos de medição utilizados tenham um desempenho analítico aceitável. Para obtê-los foi verificado o analisador hematológico Beckman Coulter LH 750 de acordo com as especificações do fabricante e os requisitos de qualidade deste laboratório. Os parâmetros de desempenho avaliados, tanto em modo manual quanto automático, foram: percentual de arrastamento, de acordo com o protocolo CLSI H26-A2, em condições de repetitibidade, precisão em condições de precisão intermediária e veracidade, conforme o protocolo CLSI EP15-A2 usando controles Bio-Rad, intervalo de medição segundo o protocolo CLSI EP6-A, limite de quantificação, de acordo com CLSI EP- 17-A2 e intervalos de referência, de acordo com o protocolo CLSI EP28-A3C. Os dados foram analisados através de LinChecker e GraphPad 5. Nos ensaios realizados foram obsevadas as especificações estabelecidas pelo fabricante, bem como a exigência de qualidade deste laboratório que é variabilidade biológica mínima. Os intervalos de referência para indivíduos adultos também foram verificados. Desta forma, foi possível realizar a verificação do analisador hematológico, que demonstra que os parâmetros analíticos avaliados têm um desempenho aceitável.

Parameters of photic resetting of the circadian system of a durnal rodent, the nile grass rat

Research on circadian rhythms has been hampered by the lack of a suitable rodent model with diurnal habits. The goal of this study was to characterize the parameters of photic entrainment of the circadian system of the Nile grass rat, a small rodent that can potentially serve as a model for the study of circadian rhythms in diurnal animals. The state of the circadian system was accessed through the rhythm of running-wheel activity and manipulated by photic stimulation (white light). The results indicated that the grass rat exhibits a robust rhythm of running-wheel activity with a mean free-running period of 23.9 hours (range: 23.6 to 24.3 hours). The animal is clearly diurnal and starts activity daily approximately 50 minutes before lights-on under an L12:D12 light-dark cycle. Its photic phase-response curve shows a dead zone during most of subjective day, a phase delay region during early subjective night (maximal delay: 1.5 hours), and a phase advance region during late subjective night (maximal advance: 2.2 hours). When exposed to constant illumination, the grass rat exhibits longer circadian periods as the intensity of illumination increases, with a ceiling between 100 and 1,000 lux. Finally, its range of entrainment extends from at least 23.0 to 25.0 hours (from 22.0 to 25.5 in some animals).

Parameters of photic resetting of the circadian system of a durnal rodent, the nile grass rat

Research on circadian rhythms has been hampered by the lack of a suitable rodent model with diurnal habits. The goal of this study was to characterize the parameters of photic entrainment of the circadian system of the Nile grass rat, a small rodent that can potentially serve as a model for the study of circadian rhythms in diurnal animals. The state of the circadian system was accessed through the rhythm of running-wheel activity and manipulated by photic stimulation (white light). The results indicated that the grass rat exhibits a robust rhythm of running-wheel activity with a mean free-running period of 23.9 hours (range: 23.6 to 24.3 hours). The animal is clearly diurnal and starts activity daily approximately 50 minutes before lights-on under an L12:D12 light-dark cycle. Its photic phase-response curve shows a dead zone during most of subjective day, a phase delay region during early subjective night (maximal delay: 1.5 hours), and a phase advance region during late subjective night (maximal advance: 2.2 hours). When exposed to constant illumination, the grass rat exhibits longer circadian periods as the intensity of illumination increases, with a ceiling between 100 and 1,000 lux. Finally, its range of entrainment extends from at least 23.0 to 25.0 hours (from 22.0 to 25.5 in some animals).

Parameters of photic resetting of the circadian system of a durnal rodent, the nile grass rat

Research on circadian rhythms has been hampered by the lack of a suitable rodent model with diurnal habits. The goal of this study was to characterize the parameters of photic entrainment of the circadian system of the Nile grass rat, a small rodent that can potentially serve as a model for the study of circadian rhythms in diurnal animals. The state of the circadian system was accessed through the rhythm of running-wheel activity and manipulated by photic stimulation (white light). The results indicated that the grass rat exhibits a robust rhythm of running-wheel activity with a mean free-running period of 23.9 hours (range: 23.6 to 24.3 hours). The animal is clearly diurnal and starts activity daily approximately 50 minutes before lights-on under an L12:D12 light-dark cycle. Its photic phase-response curve shows a dead zone during most of subjective day, a phase delay region during early subjective night (maximal delay: 1.5 hours), and a phase advance region during late subjective night (maximal advance: 2.2 hours). When exposed to constant illumination, the grass rat exhibits longer circadian periods as the intensity of illumination increases, with a ceiling between 100 and 1,000 lux. Finally, its range of entrainment extends from at least 23.0 to 25.0 hours (from 22.0 to 25.5 in some animals).