Extraction and preconcentration of lead (II) in various water and food samples by orbital shaker-assisted magnetic solid phase extraction method using a new magnetic poly linoleic acid-polystyrene-PDMS block copolymer.

The aim of this study was to develop a practical orbital shaker-assisted magnetic solid phase extraction (OSA-MSPE) method for the determination of lead by FAAS. A new magnetic poly linoleic acid-polystyrene-polydimethylsiloxane (PSt-PLina-PDMS) hydrophobic graft copolymer was synthesized and characterized by NMR, FT-IR, SEM-EDX, DSC, TGA, BET and used as adsorbent for the extraction of Pb (II). This adsorbent can be used at least 50 times without any decrease of its adsorption properties for the adsorption and elution of analyte ions. Several analytical parameters including pH, adsorbent amount, sample volume, shaking time, etc. were optimized. Multivariate optimization was used for the investigation of different parameters. The linear range at optimum operating condition was 1.7-84 µg L-1. The limit of detection (LOD) and limit of quantification (LOQ) were 0.5 µg L-1, 1.7 µg L-1, respectively. Intraday and interday relative standard deviation (RSD %), enhancement factor (EF) and adsorbent capacity were found as 1.9%, 3.3%, 166.7, 50 mg g-1, respectively. OSA-MSPE method was tested with certified reference materials including LGC-6010 (Hard Drinking Water), NCS ZC73032 Celery and CS-M-3 Control Sample Microelements in Mushroom Powder for the accuracy. Experimental results for lead were confirmed with certified values. Present method was successfully applied to various liquid and solid food samples. The OSA-MSPE method has some important features such as selective, sensitive, low LOD, LOQ and RSD, pre-concentration factor (PF) and high enhancement factor (EF). High tolerance limits against matrix ions were achieved.

Selected detrimental and essential elements in fruiting bodies of culinary and toxic medicinal macroscopic fungi growing in the Bohemian Forest, the Czech Republic.

Selected wild-growing edible fungi (Boletus edulis, Neoboletus luridiformis, Cantharellus cibarius, Macrolepiota procera, Amanita rubescens, Russula virescens, Lycoperdon perlatum, and Flammulina velutipes) along with the poisonous medicinal species Amanita muscaria were collected from five sites in the Bohemian Forest, the Czech Republic and analyzed regarding the contents of 19 elements (Ag, Al, As, Be, Ca, Cd, Co, Cr, Cu, Fe, Li, Mg, Mn, Ni, Pb, Rb, Se, Tl, and Zn) in their fruiting bodies. The contents of the elements as well as bioconcentration factors (ratios of the element content in dry matter of the mushroom to the content in the soil; BCF) were significantly species dependent. In general, the analysis revealed the most intensive accumulation of Cd, Rb, Ag, Cu, Se, and Zn in the studied mushrooms. B. edulis accumulated Ag, Se, Cd, Rb, Cu, and Zn with average BCF of 31, 25, 18, 13, 3.9, and 2.6, respectively. On the other hand, A. rubescens accumulated Cd, Rb, Ag, Cu, Zn, and As (BCF of 41, 27, 4.8, 3.3, 2.1, and 1.4). The data concerning the detrimental elements in sporocarps of edible mushrooms indicate no negative effect on human health if the fungi are consumed occasionally or as a delicacy.

Non-radioactive Rb+ Efflux Assay for Screening KATP Channel Modulators.

ATP-sensitive potassium (KATP) channels function as metabolic sensors that link cell membrane excitability to the cellular energy status by controlling potassium ion (K+) flow across the cell membrane according to intracellular ATP and ADP concentrations. As such, KATP channels influence a broad spectrum of physiological processes, including insulin secretion and cardiovascular functions. KATP channels are hetero-octamers, consisting of four inward rectifier potassium channel subunits, Kir6.1 or Kir6.2, and four sulfonylurea receptors (SURs), SUR1, SUR2A, or SUR2B. Different Kir6 and SUR isoforms assemble into KATP channel subtypes with distinct tissue distributions and physiological functions. Mutations in the genes encoding KATP channel subunits underlie various human diseases. Targeted treatment for these diseases requires subtype-specific KATP channel modulators. Rubidium ions (Rb+) also pass through KATP channels, and Rb+ efflux assays can be used to assess KATP channel function and activity. Flame atomic absorption spectroscopy (Flame-AAS) combined with microsampling can measure Rb+ in small volume, which provides an efficient tool to screen for compounds that alter KATP channel activity in Rb+ efflux assays. In this chapter, we describe a detailed protocol for Rb+ efflux assays designed to identify new KATP channel modulators with potential therapeutic utilities.

Monitoring and Health Risk Assessment of Lead and Cadmium in Date Palm Fruit Cultivars Growing in Morocco.

The present study aimed to investigate the levels of two toxic trace elements (lead (Pb) and cadmium (Cd)) in 84 date fruit samples of four varieties (Mejhoul, Bouittob, Aziza and Boufegouss) produced in four Moroccan areas, using graphite furnace atomic absorption spectrometry, and to assess their risk to the consumer health. Results showed that out of the total samples, Pb was detected in 39 date fruit samples (46.43%) in the range of 0.0001-0.118 mg/kg, with an average level of 0.016 ± 0.0005 mg/kg. For Cd levels, a total of 40 samples (47.62%) were found to be contaminated with Cd levels ranging from 0.0004 to 0.752 mg/kg, with a mean value of 0.079 ± 0.003 mg/kg. Cadmium levels exceeded the European maximum regulatory limit (MRL) of 0.020 mg/kg in 17 date samples, which represents 20.24%. However, only one sample (1.19%) was above the MRL for Pb (0.10 mg/kg) set by the Codex Alimentarius Commission (CAC). The hazard quotient (HQ) and hazard index (HI) values were found to be below 1. Furthermore, cancer risk (CR) levels exceeded the acceptable ranges. As a result, the non-carcinogenic risk assessments have concluded that consuming date fruits is safe and unlikely to harm harmful effects to the consumers. However, the date consumption may pose a significant lifetime carcinogenic risk to consumers from date-growing regions.

A spectrometric analysis of variedly purified cinnabar in a siddha drug - linga chendhooram.

OBJECTIVES: A siddha drug, linga chendhooram was prepared from cinnabar (lingam) and Citrullus colocynthis L. The transition of heavy metals concentration throughout the process were analysed to comprehend the significance of drug preparatory methods. In addition to that the main constituent cinnabar was purified from two different methods to comparatively analyse the significance of purification methods. METHODS: Atomic absorption spectrometric studies were employed to obtain the concentration of class I heavy metals (lead, arsenic, cadmium and mercury) in all five samples - raw cinnabar (R), cinnabar purified (P1 and P2) from purification method 1 and 2, linga chendhooram (D1 and D2) prepared from P1 and P2. RESULTS: Based on the permissible limits given by The Ayurvedic Pharmacopoeia of India, except cadmium the concentrations of all other three heavy metals were found to be above the limit in P1, P2, D1 and D2. According to the oral Permitted Daily Exposure values for elemental impurities given in ICH Q3D(R1) Guidelines, D1 was within the permissible limits for both single and double oral doses whereas D2 showed a slight increase in the concentration of lead in a single oral dose itself. CONCLUSIONS: The drug preparatory method showed a reduction of the heavy metals concentration and comparative analysis of the purification methods revealed a substantial reduction of heavy metals concentration in the end drug D1 when compared with D2 showing that the methods of purification and drug preparation in siddha system plays a vital role in reducing the concentrations of heavy metals accordingly.

Two-step extraction for the evaluation of metal-organic framework impregnated materials.

Metal-organic frameworks (MOFs) are widely used for gas adsorption, separation, and sensing materials. In most cases, MOFs are not used in their crystal form but as impregnated materials because the fine crystals result in high-pressure drops. One key characteristic of MOF-impregnated materials is the amount of MOF in the material. This is evaluated using the wet digestion method; however, it is limited to determining only the metal content. Moreover, some metal, denoted as free metal, will not react with ligands to form MOFs. Additionally, it is crucial to determine the ligand amount, which cannot be determined using wet digestion. In the present study, a two-step extraction method for copper (II) benzene-1,3,5-tricarboxylate (Cu-BTC MOF) impregnated materials was developed to determine the MOF formed and free metals and ligands. Various solvents were applied to evaluate the extraction efficiencies. The results led to the selection of ethanol (EtOH) for extracting free Cu2+ and BTC, while 0.3 M HNO3 was chosen to extract MOF-formed Cu2+ and BTC. The MOF-impregnated sample material was first extracted using EtOH and then 0.3 M HNO3. The Cu2+ and BTC in the obtained extract solutions, as well as EtOH and HNO3, were analyzed using flame atomic absorption spectroscopy and high-performance liquid chromatography, respectively. In standard addition tests, free and MOF-formed Cu2+ and BTC were quantitatively extracted from MOF-impregnated materials. The developed two-step analysis method was successfully applied to Cu-BTC-impregnated materials used in gas sensing.

Heavy metal analysis of water and sediments of the Kaptai Lake in Bangladesh: Contamination and concomitant health risk assessment.

In this study, concentrations of 9 heavy metals (Cr, Fe, Co, Ni, Cu, Zn, As, Cd, and Pb) in water and sediments of the Kaptai Lake were determined by neutron activation analysis and atomic absorption spectrometry techniques to study their distribution and contamination in the lake. Average concentrations of Cr and Co in sediments, and Fe and Pb in water were higher than those of some international guideline values. Different environmental pollution indexes (individual and synergistic) suggested that the sediments of Kaptai Lake are minorly enriched by As and Zn, and have low severity of contamination at most of the sampling sites. For residential receptors exposed to the heavy metals in lake water, both non-carcinogenic and carcinogenic hazards were assessed which indicated that there is no carcinogenic risk for As while Cr shows a slightly carcinogenic risk. Moreover, estimated potential ecological risks and different SQGs suggested low ecotoxicological risks in the sediments of Kaptai Lake. Multivariate statistical analyses revealed the correlation among the studied heavy metals and indicated that the origin of most of the metals is mainly lithogenic and a small number of metals (Cu and Pb) from anthropogenic sources. The results of this study will be helpful in developing a pollution control strategy for the lake.

Development of a Predictive Model for Iron Levels in Bovine Muscle Tissue Using Hair as a Predictor.

The assessment of iron levels in cattle muscle tissue is crucial for livestock management because it influences both animal health and meat quality, key factors in sustainable development. This study aimed to develop an optimal model for noninvasively predicting the iron content in Hereford cattle muscle tissue, contributing to a comprehensive understanding of the animals' elemental status. The research involved the atomic absorption analysis of muscle tissue and hair samples from cattle. A regression model was constructed using the least squares method to identify the most effective approach. These findings have ecological applications, aiding in evaluating environmental health and establishing acceptable iron thresholds for animals. The proposed mathematical model utilizing biomarkers (levels of Mg, K, Fe, Al, Cr in hair) will allow for the assessment of iron levels in cattle muscle tissue throughout the period of productive use, with the possibility of adjustment and tracking the changes in elemental status over time. The utilization of the developed method will enable the diagnosis of animal elementosis and assessment of the iron level burden. Subsequently, this will allow for the improvement of the qualitative characteristics of the final product. Thus, the obtained data contribute to fundamental knowledge regarding the content and variability of iron levels in the muscle tissue of cattle.

Acid-based deep eutectic solvents followed by GFAAS for the speciation of As(III), As(V), total inorganic arsenic and total arsenic in rice samples.

In the present study, an efficacious, safe, inexpensive and eco-friendly microextraction was provided by deep eutectic solvents based on dispersive liquid-liquid microextraction (DLLME - DES) followed by GFAAS. A series of DESs were synthesised using l-menthol as hydrogen bond acceptor (HBA) and carboxylic acids with 4, 6, 8 and 10 carbon atoms as hydrogen bond donors (HBD). The synthesised DESs were used as extractants of arsenic ions. Under optimised conditions, good linearity with coefficient of determination (r2) 0.992 and an acceptable linear range of 0.3-100 µg kg-1 was obtained. The limit of detection was 0.1 µg kg-1 (S/N = 3) for arsenite (As(III)) ions, and a high enrichment factor (EF = 200) was obtained. The enhancement factor and extraction recovery (ER%) of the method were 340 and 60%, respectively. RSDs including inter- and intra-day ranged from 3.2% to 5.8% in three examined concentrations. After a specific digestion, the capability of the synthesised DES in the extraction of As(III) from rice was tested. Total inorganic arsenic was separated similarly after reduction of arsenate (As(V)) to As(III), and As(V) concentration was calculated by difference. Using a second digestion method, total arsenic concentration (sum of organic and inorganic arsenic) in the samples was determined.

An in vitro comparison of calcium ions release and diffusion ability of calcium hydroxide-based intracanal medicament in combination with three different vehicles like propolis, chitosan, and propylene glycol.

Context: Calcium hydroxide, which is an intracanal medicament, is widely used in endodontics. Improvements can be made to its effectiveness, as calcium hydroxide is dependent on the vehicle. Aim: The study aims to compare and evaluate the release and diffusion ability of calcium hydroxide when mixed with - propolis, chitosan, and propylene glycol. Methods: For this study, 33 single-rooted extracted premolar teeth have been decoronated. After the working length and enlargement of the canals had been established, different preparations of calcium hydroxide with vehicles such as propolis, chitosan, and propylene glycol were loaded into the canals. Atomic absorption spectrophotometry was used to analyze the release of calcium ions in three groups, while a digital pH meter was used to determine an acid change. Results: Atomic absorption spectrophotometry showed sustained releases of calcium ions and the digital pH meter showed increased diffusion capacity in the propylene glycol paste group in comparison to the other two groups. Conclusion: Propylene glycol vehicle made it easier to enter calcium hydroxide into the dentinal tubules.

Chemical speciation and comprehensive risk assessment of metals in sediments from Nabq protectorate, the Red Sea using individual and synergistic indices.

The study evaluates metal concentrations, distributions, contamination, risk, sources, fractionation, and mobility in Nabq Protectorate sediments, revealing a metal content order of Fe, Mn, Pb, Ni, and Cd. Metals are dominated by residual fractions, with Cd (83.70 %) > Ni (82.98 %) > Pb (80.96 %) > Fe (80.31 %) > Mn (76.65 %) reflecting the natural sources of investigated metals. Mn (23.35 %) was the most abundant mobile metal, and the sediments of the protectorate had low toxicity and moderate risk according to the synergistic indices (1 ≤ mRAC<10 and ERM; 5-10). The results from the proposed individual indices showed that Mn, Fe, and Pb are the most bioavailable (BIM 0.1-0.4), Cd, Mn, Ni, Fe, and Pb are of moderate mobility (MIM 0.1-0.4), and Cd is the most available (ARIM 5-10), with Cd posing the most ecological risk. The total hazard quotient (THQ) for child was greater than one, exposure to manganese through ingestion and skin contact while swimming may endanger human health.

CoSn(OH)6 nanocubes as a solid sorbent for the effective preconcentration of copper ions in cinnamon (Cinnamomum zeylanicum) extract.

This study was aimed at developing a simple and efficient CoSn(OH)6 nanocubes-based preconcentration method for the preconcentration of copper ions from cinnamon extracts for determination by flame atomic absorption spectrometry. The cube-shaped sorbent was synthesized using the simple stoichiometric co-precipitation method under ambient conditions. Experimental factors of the method were evaluated with a comprehensive optimization approach to maximize the extraction efficiency for the analyte. Under the optimal conditions, the limit of detection (LOD), limit of quantitation (LOQ), and linear dynamic range were recorded as 0.98 µg/L, 3.28 µg/L, and 4.0-75 µg/L, respectively. The enhancement factor was calculated as 101.6-fold by comparing the LODs of the optimized and direct analysis systems. Percent recoveries were found to be within an acceptable range (77.6-115 %), with high repeatability using matrix matching calibration strategy. Results validated the proposed method as a highly efficient extraction approach for the monitoring of copper ions in herbal cinnamon extracts.

Rapid and sensitive in situ detection of heavy metals in fish using enhanced Raman spectroscopy.

Heavy metals have been widely applied in industry, agriculture, and other fields because of their outstanding physics and chemistry properties. They are non-degradable even at low concentrations, causing irreversible harm to the human and other organisms. Therefore, it is of great significance to develop high accuracy and sensitivity as well as stable techniques for their detection. Raman scattering spectroscopy and atomic absorption spectrophotometer (AAS) were used parallelly to detect heavy metal ions such as Hg, Cd, and Pb of different concentrations in fish samples. The concentration of the heavy metals is varied from 5 ppb to 5 ppm. Despite the satisfactory recoveries of AAS, their drawbacks are imperative for an alternative technique. In Raman scattering spectroscopy, the intensities and areas of the characteristic peaks are increased with increasing the concentration of the heavy metals. For Hg concentration ≥ 1 ppm, a slight shift is observed in the peak position. The obtained values of peak intensity and peak area are modeled according to Elvoich, Pseudo-first order, Pseudo-second order, and asymptotic1 exponential model. The best modeling was obtained using the Elovich model followed by the asymptotic1 exponential model. The introduced Raman spectroscopy-based approach for on-site detection of trace heavy metal pollution in fish samples is rapid, low-cost, and simple to implement, increasing its visibility in food safety and industrial applications.

Health effects of heavy metals in meat and poultry consumption in Noakhali, Bangladesh.

This study examined the quantities of heavy metals (Cd, Cr, Pb, Ni, Fe, and Cu) in commercially available meat, poultry, and game products in Noakhali, Bangladesh, and their potential health effects, as heavy metal contamination poses a significant food safety risk to human health Atomic Absorption Spectrophotometry was used to analyze heavy metals, and the health risk assessment was based on Estimated Daily Intake (EDI), Targeted Hazard Quotient (THQ), Total THQ, and Total Carcinogenic Risk (TCR). Most samples exceeded Maximum Allowable Concentrations (MAC) for heavy metals. The EDI value of Cd, Pb, and Cr for duck liver, goat liver, and pigeon brain, were higher than the Maximum Tolerable Dietary Intake (MTDI). Children had 1.28 times higher HI values than an adult. The calculation of THQ of all elements in adults and children was in the order of Cu; Pb; Ni; Cr; Cd; and Fe. The calculated TTHQ values were in the range of 0.051 to 1.988 and 0.047 to 3.975 for adults and children, respectively. The TCR values for Cd in poultry liver, brain, and meat, Sonali chicken, cow, pigeon, duck, and goat liver were higher than the reference value for adults and children, suggesting a potential cancer risk. The average exposure to lead leads to an increase in blood pressure by 0.47 mmHg and a decrease in IQ score by 1.94 points. The present study reveals the need to determine strong relationships between heavy metal exposure and food supply.

Switchable hydrophilicity solvent-assisted solidified floating organic drop microextraction for separation and determination of arsenic in water and fish samples.

The aim of the current study was to separate and determine arsenic in water and fish samples using a novel and green solidified floating organic drop microextraction (SFODME), which is based on switchable hydrophilicity solvent (SHS)-assisted procedure followed by hydride generation atomic absorption spectrometry (HG-AAS). The 4-((2-hydroxyquinoline-7-yl)diazenyl)-N-(4-methylisoxazol-3-yl)benzene sulfonamide (HDNMBA) and tertiary amine (4-(2-aminoethyl)-N,N-dimethylbenzylamine (AADMBA) were used as ligand and SHS, respectively. The use of SHS promotes quantitative extraction of arsenic complexes into an extraction solvent (1-undecanol). Some factors that impact extraction recovery were studied. Under optimal conditions, the limit of detection (LOD) and limit of quantification (LOQ) were 0.005 µg L-1 and 0.015 µg L-1, respectively. The calibration graph was linear up to 900.0 µg L-1 arsenic, with the enrichment factor is 267. The proposed SHS-SFODME methodology for arsenic quantification in water and fish samples was successfully implemented. The environmental friendliness and safety of proposed method were approved by the Analytical Greenness Calculator (AGREE) and the Blue Applicability Grade Index (BAGI) tools.

Determination of copper and cobalt in different tea samples at trace levels by FAAS after preconcentration with a novel iron PAMAM-OH-encapsulated magnetic nanoparticle as SPE sorbent.

Environmental contamination caused by heavy metals is a significant global concern. The presented study investigated the efficiency of iron PAMAM-OH encapsulated magnetic nanoparticles (Fe-MNP-G2-OH) as sorbent for the preconcentration of copper and cobalt from tea samples. High metal-chelating ethylenediamine core polyamidoethanol generation-2 (PAMAM-G2-OH) was encapsulated with iron oxide (Fe3O4) to synthesize the sorbent. Limit of detection (LOD) values for copper and cobalt extracted and detected by the developed Fe-MNP-G2-OH -SPE-FAAS method were 0.52 and 1.1 µg L-1, respectively. There were 230- and 101-fold improvement in detection limits for copper and cobalt, respectively, when compared to direct FAAS measurement. The percent recovery results for the analytes in green and black tea samples ranged from 93 to 107%, with low relative standard deviation (%RSD) values. The synthesis of nanoparticle was carried out through a unique method, which was characterized by thermogravimetric analysis (TGA), scanning electron microscope (SEM), and Fourier transform infrared spectroscopy (FTIR) methods. The analytical results demonstrated the applicability and effectiveness of Fe-MNP-G2-OH nanoparticles on the preconcentration of copper and cobalt from tea samples and the developed method is suitable for the trace detection of heavy metals by FAAS method. To the best our knowledge, this is the first study where copper and cobalt in green and black tea samples were extracted by Fe-MNP-G2-OH adsorbent and precipitation of the adsorbent during its synthesis was carried out in acetone medium rather than aqueous one.

Micro solid phase extraction of lead and cadmium using functionalized nanodiamonds@CuAl2O4@HKUST-1 nanocomposite for FAAS analysis in food and water samples.

This study focuses on the development and application of a novel nanocomposite (functionalized nanodiamonds@CuAl2O4@HKUST-1)-based µ-SPE method for the sensitive and selective extraction of Pb and Cd from food and water samples. The technique offers high sensitivity and selectivity, allowing accurate measurement of these metals at trace levels. The detection limit is 0.031 µg kg-1 for Cd and 0.052 µg kg-1 for Pb, with a relative standard deviation of 1.7 % for Cd and 4.8 % for Pb. The method was successfully applied to real samples and efficiently quantified Pb and Cd in food and natural water samples. The highest concentrations were found in red lentils (0.274 µg kg-1 Pb) and fresh mint (0.197 µg kg-1Cd), but still below recommended limits set by FAO/WHO (300 µg kg-1 for Pb and 200 µg kg-1 for Cd). It promises to ensure food safety, monitor environmental contamination, and informs regulatory decisions to protect public health.

Detection of Trace Elements Se, Cu, and Zn Levels in Biological Samples of Drug Abusers: Halani Sindh, Pakistan.

Illicit drug use has become a global issue, posing significant health, socioeconomic, and cultural risks. The study examined essential trace metals: selenium, zinc, and copper in blood concentrations, as well as in serum and scalp hair samples, from 240 male drug-abuse subjects/patients aged 18-45, categorized into three age groups. The study compared 45 healthy subjects of the same age group using an acid digestion method supported by a microwave oven during sample preparation. The technique of atomic absorption spectrometry was employed to identify essential and toxic elements, utilizing certified reference materials for accuracy. According to a recent study, plasma zinc and selenium concentrations in drug abusers are lower than those in referent subjects, potentially increasing vulnerability to infection due to poor nutritional status or other contaminants.

Phytochemical studies of white mulberry fruits (Morus alba L.).

Medicinal preparations made from plant materials have been widely used for many years due to their high pharmacological efficacy and safety of use. Therefore, a study of white mulberry fruits (Morus alba L.) for the content of substances is very important for the pharmaceutical industry such as flavonoids, alkaloids, polysaccharides, minerals, vitamins, and amino acids. White mulberry has a wide distribution area around the world, including in Kazakhstan, especially in the southern regions of the country (Almaty, Zhambyl, and Turkestan regions). The composition of the fruits of this plant is significantly influenced by the area where the trees grow, and therefore, the establishment of a specific composition of biologically active substances is very important. In the course of this study, such methods as gas chromatography were used-mass spectrometry of an extract obtained using carbon dioxide under subcritical conditions, atomic absorption, gravimetric, and spectrophotometric methods. As a result, for the first time in Kazakhstan, the composition of white mulberry fruits (Morus alba L.), namely, biologically active substances, has been identified, such as alkaloids, flavonoids, vitamins, macro- and microelements, and amino acids and fatty acids; in addition, the percentage composition of the above compounds has been determined. The results of the study show a comparative analysis of the component composition of white mulberry fruits (Morus alba L.) in various areas of tree growth, including outside of Kazakhstan. The obtained data testify to the great possibilities of using this raw material in medicine, pharmacology, and the food industry.

Evidence of direct interaction between cisplatin and the caspase-cleaved prostate apoptosis response-4 tumor suppressor.

Prostate apoptosis response-4 (Par-4) tumor suppressor protein has gained attention as a potential therapeutic target owing to its unique ability to selectively induce apoptosis in cancer cells, sensitize them to chemotherapy and radiotherapy, and mitigate drug resistance. It has recently been reported that Par-4 interacts synergistically with cisplatin, a widely used anticancer drug. However, the mechanistic details underlying this relationship remain elusive. In this investigation, we employed an array of biophysical techniques, including circular dichroism spectroscopy, dynamic light scattering, and UV-vis absorption spectroscopy, to characterize the interaction between the active caspase-cleaved Par-4 (cl-Par-4) fragment and cisplatin. Additionally, elemental analysis was conducted to quantitatively assess the binding of cisplatin to the protein, utilizing inductively coupled plasma-optical emission spectroscopy and atomic absorption spectroscopy. Our findings provide evidence of direct interaction between cl-Par-4 and cisplatin, and reveal a binding stoichiometry of 1:1. This result provides insights that could be useful in enhancing the efficacy of cisplatin-based and tumor suppressor-based cancer therapies.