Unveiling the overlooked: Current and future distribution dynamics of kissing bugs and palm species linked to oral Chagas disease transmission.

Chagas disease, a neglected global health concern primarily transmitted through the bite and feces of kissing bugs, has garnered increasing attention due to recent outbreaks in northern Brazil, highlighting the role of oral transmission facilitated by the kissing bugs species Rhodnius robustus and Rhodnius pictipes. These vectors are associated with palm trees with large crowns, such as the maripa palm (Attalea maripa) and moriche palm (Mauritia flexuosa). In this study, we employ maximum entropy (MaxEnt) ecological niche models to analyze the spatial distribution of these vectors and palm species, predicting current and future climate suitability. Our models indicate broader potential habitats than documented occurrences, with high suitability in northern South America, southern Central America, central Africa, and southeast Asia. Projections suggest increased climate suitability by 2040, followed by a reduction by 2080. This study identifies present and future areas suitable for kissing bugs and palm tree species due to climate change, aiding in the design of prevention and management strategies.

Cytotoxicity, anti-inflammatory effect, and acute oral toxicity of a novel Attalea phalerata kernel oil-loaded nanocapsules.

The kernel oil of the Attalea phalerata Mart. Ex Spreng (Acurí) is traditionally used in several Latin American countries to treat respiratory problems, inflammation, and fever. However, it cannot be found on the literature any attend to use this oil in pharmaceutical formulation. In this paper, it was developed Acurí oil-loaded nanocapsules, and it was evaluated the cytotoxicity against cancer cells, the antinflammatory activity and the oral acute toxicity in rats. Acurí oil contains lauric acid as the predominant saturated fatty acid (433.26 mg/g) and oleic acid as the main unsaturated fatty acid (180.06 mg/g). The Acurí oil-loaded nanocapsules showed a size of 237 nm, a polydispersity index of 0.260, and a high ζ-potential of -78.75 mV. It was obtained an encapsulation efficiency of 88.77%, and the nanocapsules remain stable on the shelf for 180 days. The nanocapsules showed a rapid release profile (98.25% in 40 minutes). Nanocapsules at a dose of 10 mg/kg exhibit an anti-inflammatory effect similar to indomethacin at the same dose. The nanocapsules showed excellent antiproliferative effect and selectivity index against prostate tumor cells (IC50 2.09 µg/mL, SI=119.61) and kidney tumor cells (IC50 3.03 µg/mL, SI=82.50). Both Acurí oil and Acurí oil-loaded nanocapsules are nontoxic at a dose of 2000 mg/kg. Additionally, they reduce serum triglyceride and total cholesterol levels in rat and could find application in nutraceutical formulations. The Acurí oil-loaded nanocapsules emerge as a promising candidate for new antitumor therapies.

The monodominance of Acuri in Pantanal formations: allelochemical effects of its leaves and the presence of Acurizais / A monodominância do Acuri em formações do Pantanal: efeitos aleloquímicos de suas folhas e a presença de acurizais

The presence of monodominant vegetative formations almost exclusively composed of Acuri palm trees (Attalea phalerata) stands out in some regions of the Pantanal Sul-Mato-Grossense. These formations are generally associated with anthropic, edaphic and/or hydrological factors. However, little is known about the effect of allelopathy on the formation and maintenance of these areas. Herein, we investigated the chemical composition of A. phalerata aqueous leaf extract and the potential allelopathic effects on germination and growth of target L. sativa, L. esculentum and S. obtusifolia species. Thus, extracts at concentrations of 0, 2.5, 5, 10, 15, and 20% were used for germination and growth bioassays with a completely randomised design in a germination chamber and greenhouse. The results showed that the A. phalerata extracts negatively affected the germination speed index and mean germination time of the target species and positively affected seedling length under controlled conditions and were also stimulated in the greenhouse. Thus, the formation of Acurizals can be related to the presence of secondary metabolites in the leaves, in addition to other environmental factors.

No Pantanal Sul-Mato-Grossense se destaca, em determinadas regiões, a presença de formações vegetacionais monodominantes, compostas quase que exclusivamente por uma espécie de palmeira, o Acuri (Attalea phalerata). Normalmente estas formações estão associadas a fatores antrópicos, edáficos e/ou hidrológicos. Porém pouco se sabe sobre o efeito da alelopatia na formação e manutenção destas áreas. Desta maneira, objetivou-se estudar a composição química dos extratos aquosos das folhas de A. phalerata e seus possíveis efeitos alelopáticos na germinação e crescimento das espécies-alvo, alface, tomate e fedegoso. Para os bioensaios de germinação e crescimento, foram utilizados extratos nas concentrações de 0, 2,5, 5, 10, 15 e 20%, com delineamento inteiramente casualizado, em câmara de germinação e casa de vegetação. Os resultados demostraram que em condições controladas (câmara de germinação), os extratos de A. phalerata afetaram negativamente o índice de velocidade de germinação e o tempo médio de geminação das espécies-alvo e positivamente, o comprimento das plântulas, que também foram estimuladas em casa de vegetação, indicando a presença de fitormônios. Desta maneira, a formação de Acurizais pode estar relacionada à presença de metabolitos secundários nas folhas, além de outros fatores ambientais.

Emerging Lipids from Arecaceae Palm Fruits in Brazil.

Arecaceae palm tree fruits (APTFs) with pulp or kernel rich in oil are widely distributed in six Brazilian biomes. APTFs represent a great potential for the sustainable exploitation of products with high added value, but few literature studies have reported their properties and industrial applications. The lack of information leads to underutilization, low consumption, commercialization, and processing of these fruit species. This review presents and discusses the occurrence of 13 APTFs and the composition, physicochemical properties, bioactive compounds, and potential applications of their 25 oils and fats. The reported studies showed that the species present different lipid profiles. Multivariate analysis based on principal component analysis (PCA) and hierarchical cluster analysis (HCA) indicated a correlation between the composition of pulp and kernel oils. Myristic, caprylic, capric, and lauric acids are the main saturated fatty acids, while oleic acid is the main unsaturated. Carotenoids and phenolic compounds are the main bioactive compounds in APTFs, contributing to their high oxidative stability. The APTFs oils have a potential for use as foods and ingredients in the cosmetic, pharmaceutical, and biofuel industries. However, more studies are still necessary to better understand and exploit these species.

Inclusion of babassu bran produced in milk production in Amazonia.

One important species for extraction and subsistence of traditional populations in Brazil is the babassu palm (Attalea speciosa Mart ex Spreng). As a by-product of the babassu oil socio-productive chain in Amazonia region, the residual bran needs to be better used and could represent an additional source of income for coconut breakers, and a local alternative in the composition of diets for dairy cows. The objective of this work was to evaluate the effect of a diet containing babassu bran on the intake and digestibility of cows, their production and milk composition, in the Amazon region. The experimental trial was arranged in a Cross over design, composed of fourteen animals, two treatments and two periods of 28 days (21 days for adaptation to diets and 7 days for data collection). Treatments were concentrate without babassu bran (CNV) and with babassu bran (BBS). Milk composition did not differ significantly between treatments. The inclusion of 35.6% BBS in feed ration supported an average daily milk production of 18.2 kg/day, while CNV produced 19.8 kg/day. There was lower feed consumption for BBC, but equal to that of CNV when comparing liters of milk produced per kg of ingested concentrate (1:3.09 CNV × 1:3.07 BBS), which demonstrates that treatments did not differ in productive efficiency. Our results bring the possibility of a better use of babassu bran. This alternative could improve the quality of life of rural populations, while being linked to the conservation of native ecosystems and the sustainable use of socio-biodiversity products.

Effect of Babassu Mesocarp As a Food Supplement During Resistance Training.

The population widely uses babassu mesocarp (Attalea speciosa) as food and medicine. This study evaluated the use of babassu mesocarp as a food supplement during resistance training (RT). Male Swiss mice, 60 days old (weight 35-40 g), were divided into four groups (n = 8): control, untreated and untrained; babassu (babassu aqueous extract [BAE]), treated orally with aqueous extract of babassu mesocarp (25 mg/kg), five times a week, for 8 weeks; training (RT), submitted to RT consisting of stair climbing with progressive loads; and resistance training treated with babassu aqueous extract (RTBAE): RT and treatment with BAE. After 8 weeks, we analyzed the biochemistry of serum, the immunological, and histological parameters. The RT group showed maximum strength after the second week. A reduction in body weight, retroperitoneal and interstitial fat deposits, and activated helper T lymphocytes (TCD4+ CD69+) occurred in RT and RTBAE groups. The RTBAE group showed increased levels of aspartate aminotransferase, alanine aminotransferase, and macrophage and helper T lymphocyte count, whereas a reduction occurred in triglyceride levels and the total number of lymphocytes. Supplementation with BAE always reduced cholesterol and the population of activated macrophages but increased activated B lymphocytes and interleukin-6 levels. The combination of supplementation and RT resulted in a decreased production of tumor necrosis factor-α. We propose the use of babassu mesocarp as a food supplement during exercise because of its immunomodulatory effect on lymphocyte and macrophage populations and cytokine production. The additional impact on the control of cholesterol and triglyceride levels suggests its use, particularly for the treatment of dyslipidemias.

Landscape disturbance impacts on Attalea butyracea palm distribution in central Panama.

BACKGROUND: Increased Attalea butyracea palm propagation, notable for its role as key habitat for the primary Chagas disease vector in Panama, has been linked to landscape disturbance in single-palm observations in this region. Close proximity of these palms to human dwellings is proposed to increase risk of Chagas disease transmission from sylvatic transmission cycles to domestic transmission involving human populations. This study examines the relationship between landscape disturbance and mature A. butyracea spatial distribution, density, and proximity to human populations and vector and reservoir species' movement corridors at a regional scale in a 300 km2 heterogeneous tropical landscape in central Panama. METHODS: We remotely identified the locations of over 50,000 mature A. butyracea palms using high-resolution WorldView2 satellite imagery. A local Getis-Ord Gi* spatial analysis identified significant clusters of aggregated palms. Associations between palm and cluster abundance and a landscape disturbance gradient, derived from official Panama land cover data, were tested using Chi-square tests for Homogeneity and Z-test for proportions. Kruskall-Wallis non-parametric analysis of variance tests were run to assess whether palm cluster area varied by disturbance level, or whether disturbance was associated with proximity of palms and palm clusters to susceptible populations or vector movement corridors. RESULTS: Our findings indicate a regional relationship between landscape disturbance and A. butyracea occurrence. We observe a significant increase in both individual and clustered A. butyracea in secondary forest, but a reduction of palms in agricultural settings. We do not detect evidence of any reduction in abundance of palms in residential settings. The majority of residential and commercial buildings in our study area are within vector flight distance of potential vector habitat in palm crowns. CONCLUSIONS: We observe probable anthropogenic elimination of A. butyracea palms in agricultural, but not residential, settings. Even in heavily deforested regions, significant concentrations of mature palms remain in close proximity to human establishments.

Litter decomposition and nutrient release dynamics of leaves and roots of the babassu palm in eastern Amazonia

The ruderal babassu palm (Attalea speciosa) is expanding on large areas of degraded Amazon landscapes. Decomposition of leaves and roots is in the center of plant:soil interactions. We evaluated decomposition and nutrient concentrations of leaves and fine roots of babassu in comparison with two exotic reference species, Acacia mangium (slow degradability) and Leucaena leucocephala (fast degradability), in a 138-day litterbag assay carried out in secondary forest stands of different age and babassu abundance. We chose 4-mm over 2-mm mesh litterbags based on a pilot study. Babassu leaves degraded slower than leaves of A. mangium and L. leucocephala, and also had lower nitrogen, phosphorus and calcium concentrations in all stages of decomposition. By contrast, potassium concentrations in babassu leaves were higher than in both reference species at 0 and 50 days. Roots of all three species decomposed slower than leaves. Compared to the leaves, both biomass loss and nutrient concentrations differed less between babassu and reference-species roots, except for lower nitrogen concentration in babassu roots. Leaf-litter decomposition of all three species was significantly faster in old than in young secondary forest, suggesting an acceleration of decomposition along succession. Babassu leaves decomposed faster in old babassu-dominated than non-dominated secondary forest, pointing to the existence of specialized decomposer communities in babassu-dominated stands. (AU)

Pretreatment Affects Activated Carbon from Piassava.

The specificity of activated carbon (AC) can be targeted by pretreatment of the precursors and/or activation conditions. Piassava (Leopoldinia piassaba and Attalea funifera Martius) are fibrous palms used to make brushes, and other products. Consolidated harvest and production residues provide economic feasibility for producing AC, a value-added product from forest and industrial residues. Corona electrical discharge and extraction pretreatments prior to AC activation were investigated to determine benefits from residue pretreatment. The resulting AC samples were characterized using elemental analyses and FTIR and tested for efficacy using methylene blue and phenol. All resulting AC had good adsorbent properties. Extraction as a pretreatment improved functionality in AC properties over Corona electrical discharge pretreatment. Due to higher lignin content, AC from L. piassaba had better properties than that from A. funifera.

Effects of the dietary inclusion of babassu oil or buriti oil on lamb performance, meat quality and fatty acid composition.

The effects of adding babassu oil (BAO) or buriti oil (BUO) to lamb diets, on performance, carcass characteristics, meat quality and fatty acid (FA) composition were evaluated. Feeding BAO reduced (P = .02) dry matter intake, kidney fat and dressing percentage, but did not change energy intake and performance. Meat pH, color, protein content and sensorial evaluation were not affected by diet. However, BUO increased (P = .02) intramuscular and subcutaneous fat contents, but decreased shear force. BAO increased (P < .05) trans-monounsaturated FA, total biohydrogenation intermediates (BHI) and the t10:t11 ratio, in meat and subcutaneous fat, but decreased total FA and cis-monounsaturated FA, did not change SFA, and increased (P = .04) PUFA in meat. BUO supplementation promoted the highest (P < .05) SFA and total FA content in subcutaneous fat but did not change PUFA. BAO can be used as an alternative energy source for growing lambs, but does not improve the meat FA composition.

Litter decomposition and nutrient release dynamics of leaves and roots of the babassu palm in eastern Amazonia / Decomposição de serrapilheira e dinâmica de nutrientes de folhas e raízes da palmeira babaçu na periferia leste da Amazônia

The ruderal babassu palm (Attalea speciosa) is expanding on large areas of degraded Amazon landscapes. Decomposition of leaves and roots is in the center of plant:soil interactions. We evaluated decomposition and nutrient concentrations of leaves and fine roots of babassu in comparison with two exotic reference species, Acacia mangium (slow degradability) and Leucaena leucocephala (fast degradability), in a 138-day litterbag assay carried out in secondary forest stands of different age and babassu abundance. We chose 4-mm over 2-mm mesh litterbags based on a pilot study. Babassu leaves degraded slower than leaves of A. mangium and L. leucocephala, and also had lower nitrogen, phosphorus and calcium concentrations in all stages of decomposition. By contrast, potassium concentrations in babassu leaves were higher than in both reference species at 0 and 50 days. Roots of all three species decomposed slower than leaves. Compared to the leaves, both biomass loss and nutrient concentrations differed less between babassu and reference-species roots, except for lower nitrogen concentration in babassu roots. Leaf-litter decomposition of all three species was significantly faster in old than in young secondary forest, suggesting an acceleration of decomposition along succession. Babassu leaves decomposed faster in old babassu-dominated than non-dominated secondary forest, pointing to the existence of specialized decomposer communities in babassu-dominated stands.(AU)

A palmeira ruderal babaçu (Attalea speciosa) se expande em grandes áreas da Amazônia já desmatada e degradada. A decomposição é chave na dinâmica sucessional e nas interações planta:solo. Avaliamos decomposição e concentração de nutrientes de folhas e raízes finas de babaçu e de duas espécies exóticas de referência, Acacia mangium (decomposição lenta) e Leucaena leucocephala (decomposição rápida), em capoeiras de diferente idade e grau de dominância de babaçu usando litterbags durante 138 dias. Usamos litterbags com malha de 4 mm em vez de 2 mm, com base em um estudo-piloto. As folhas de babaçu se decompuseram mais lentamente que as de A. mangium e L. leucocephala, e apresentaram concentrações mais baixas de nitrogênio, fósforo e cálcio em todos os estágios de decomposição. Em contraste, a concentração de potássio em folhas de babaçu foi mais alta que nas espécies de referência aos 0 e 50 dias. Raízes se decompuseram mais lentamente que folhas nas três espécies. Tanto a perda de massa, como a concentração de nutrientes diferiram menos entre as raízes que entre as folhas de babaçu e as espécies de referência, exceto a concentração de nitrogênio nas raízes de babaçu. A decomposição foliar das três espécies foi significantemente mais rápida em capoeira velha que em capoeira jovem, sugerindo uma aceleração da decomposição ao longo dos estágios sucessionais. A decomposição foliar do babaçu foi mais rápida em capoeira velha com alta dominância de babaçu que em capoeira velha com baixa dominância, apontando para a existência de comunidades especializadas de decompositores em áreas dominadas por babaçu.(AU)

First Study on the Oxidative Stability and Elemental Analysis of Babassu (Attalea speciosa) Edible Oil Produced in Brazil Using a Domestic Extraction Machine.

Interest in edible oil extraction processes is growing interest because the final nutritional quality of the extracted oil depends on the procedure used to obtain ir. In this context, a domestic cold oil press machine is a valuable tool that avoids the use of chemicals during oil extraction, in an environmentally friendly way. Although babassu (Attalea speciosa) oil is economically important in several Brazilian regions due to its nutritional and healthy features, few studies have been conducted on the chemical composition and stability of babassu oils extracted by cold pressing. Babassu oil's major constituents are saturated fatty acids (~86.42%), with the most prevalent fatty acids being lauric (~47.40%), myristic (15.64%), and oleic (~11.28%) acids, respectively, within the recommended range by Codex Alimentarius, presenting atherogenicity and thrombogenicity indexes favorable for human consumption. Peroxide value, Rancimat, and TGA/DSC results indicated that babassu oil is stable to oxidation. Also, macro- (Na, K, Ca, Mg, P) and micro-elements (Fe, Mn, Cr, Se, Al, and Zn) of babassu oil were determined, revealing levels below the tolerable upper intake level (ULs) for adults. These findings demonstrated that cold-press extraction using a domestic machine yielded a high-quality oil that kept oil chemical composition stable to oxidation with natural antioxidants.

Regional biogeography of microbiota composition in the Chagas disease vector Rhodnius pallescens.

BACKGROUND: Triatomine bugs are vectors of the protozoan parasite Trypanosoma cruzi, which causes Chagas disease. Rhodnius pallescens is a major vector of Chagas disease in Panama. Understanding the microbial ecology of disease vectors is important in the development of vector management strategies that target vector survival and fitness. In this study we examined the whole-body microbial composition of R. pallescens from three locations in Panama. METHODS: We collected 89 R. pallescens specimens using Noireau traps in Attalea butyracea palms. We then extracted total DNA from whole-bodies of specimens and amplified bacterial microbiota using 16S rRNA metabarcoding PCR. The 16S libraries were sequenced on an Illumina MiSeq and analyzed using QIIME2 software. RESULTS: We found Proteobacteria, Actinobacteria, Bacteroidetes and Firmicutes to be the most abundant bacterial phyla across all samples. Geographical location showed the largest difference in microbial composition with northern Veraguas Province having the most diversity and Panama Oeste Province localities being most similar to each other. Wolbachia was detected in high abundance (48-72%) at Panama Oeste area localities with a complete absence of detection in Veraguas Province. No significant differences in microbial composition were detected between triatomine age class, primary blood meal source, or T. cruzi infection status. CONCLUSIONS: We found biogeographical regions differ in microbial composition among R. pallescens populations in Panama. While overall the microbiota has bacterial taxa consistent with previous studies in triatomine microbial ecology, locality differences are an important observation for future studies. Geographical heterogeneity in microbiomes of vectors is an important consideration for future developments that leverage microbiomes for disease control.

Silage of the agro-industrial co-product of babassu palm heart processing in sheep feed / Silagem de coproduto da industrialização do palmito de babaçu na alimentação de ovinos

The inner bark or less tender sheath generated from industrial manufacture of canned babassu palm (Attalea speciosa) heart is inadequate for human consumption, often being discarded inappropriately and giving rise to environmental pollution. Accordingly, the aim of this study was to assess the intake and digestibility in sheep fed on silage of the agro-industrial co-product of babassu palm (ICBP)enriched with different additives, or on sugarcane silage. Fifteen Santa Inês sheep were distributed in a completely randomized design, consisting of five treatments and three replicates. The treatments comprised babassu palm silage enriched with broken maize (BM), broken rice (BR), cassava scrapings (CS), coffee husk combined with maize kernel (CC), and sugarcane silage (SC), all supplemented with concentrate, at a voluminous:concentrate ratio of 65:35. Silages based on ICBP and SC had similar intake of dry matter (DM), organic matter (OM), crude protein (CP), ether extract (EE), and non-fibrous carbohydrate (NFC). Increasing the additive fraction in ICBP silages favored the digestibility of DM, OM, CP, NFC, neutral detergent fiber (NDF), and total digestible nutrients (TDN). Our results indicated that silage based on additive-enriched ICBP can be used in the diet of ruminants, especially those based on broken maize and cassava scrapings that provide superior nutritional quality compared to the other silages assessed.(AU)

No processo industrial de fabricação de palmito babaçu (Attalea speciosa) em conserva, a entrecasca ou bainha menos tenra, apresenta características impróprias à alimentação humana, sendo muitas vezes descartada de forma inadequada, oferecendo riscos de poluição ambiental. De tal forma, objetivou-se avaliar o consumo e a digestibilidade em ovinos recebendo silagens de coproduto da industrialização de palmito babaçu (CIPB) com diferentes aditivos ou silagem de cana-de-açúcar. Utilizou-se 15 ovelhas da raça Santa Inês, distribuídas em delineamento inteiramente casualizado, constituindo de cinco tratamentos e três repetições. Os tratamentos compreendiam dietas de silagem de babaçu enriquecidas com quirera de milho (QM), quirera de arroz (QA), raspa de mandioca (RM), casca de café combinada com quirera de milho (CC), e silagem de cana-de-açúcar (CA), todos complementadas com concentrado, na relação volumoso:concentrado, 65:35. Silagens a base de CIPB e CA tiveram consumos de matéria seca (MS), matéria orgânica (MO), proteína bruta (PB), extrato etéreo (EE) e carboidrato não-fibroso (CNF) semelhantes. O incremento de aditivos às silagens de CIPB favoreceram a digestibilidade da MS, MO, PB, CNF, fibra em detergente neutro (FDN) e de nutrientes digestíveis totais (NDT). Silagem a base de CIPB pode ser utilizada na dieta de ruminantes com a inclusão de aditivos, especialmente quirera de milho e raspa de mandioca, que conferem qualidade nutricional superior às demais silagens avaliadas.(AU)

Silage of the agro-industrial co-product of babassu palm heart processing in sheep feed / Silagem de coproduto da industrialização do palmito de babaçu na alimentação de ovinos

ABSTRACT: The inner bark or less tender sheath generated from industrial manufacture of canned babassu palm (Attalea speciosa) heart is inadequate for human consumption, often being discarded inappropriately and giving rise to environmental pollution. Accordingly, the aim of this study was to assess the intake and digestibility in sheep fed on silage of the agro-industrial co-product of babassu palm (ICBP)enriched with different additives, or on sugarcane silage. Fifteen Santa Inês sheep were distributed in a completely randomized design, consisting of five treatments and three replicates. The treatments comprised babassu palm silage enriched with broken maize (BM), broken rice (BR), cassava scrapings (CS), coffee husk combined with maize kernel (CC), and sugarcane silage (SC), all supplemented with concentrate, at a voluminous:concentrate ratio of 65:35. Silages based on ICBP and SC had similar intake of dry matter (DM), organic matter (OM), crude protein (CP), ether extract (EE), and non-fibrous carbohydrate (NFC). Increasing the additive fraction in ICBP silages favored the digestibility of DM, OM, CP, NFC, neutral detergent fiber (NDF), and total digestible nutrients (TDN). Our results indicated that silage based on additive-enriched ICBP can be used in the diet of ruminants, especially those based on broken maize and cassava scrapings that provide superior nutritional quality compared to the other silages assessed.

RESUMO: No processo industrial de fabricação de palmito babaçu (Attalea speciosa) em conserva, a entrecasca ou bainha menos tenra, apresenta características impróprias à alimentação humana, sendo muitas vezes descartada de forma inadequada, oferecendo riscos de poluição ambiental. De tal forma, objetivou-se avaliar o consumo e a digestibilidade em ovinos recebendo silagens de coproduto da industrialização de palmito babaçu (CIPB) com diferentes aditivos ou silagem de cana-de-açúcar. Utilizou-se 15 ovelhas da raça Santa Inês, distribuídas em delineamento inteiramente casualizado, constituindo de cinco tratamentos e três repetições. Os tratamentos compreendiam dietas de silagem de babaçu enriquecidas com quirera de milho (QM), quirera de arroz (QA), raspa de mandioca (RM), casca de café combinada com quirera de milho (CC), e silagem de cana-de-açúcar (CA), todos complementadas com concentrado, na relação volumoso:concentrado, 65:35. Silagens a base de CIPB e CA tiveram consumos de matéria seca (MS), matéria orgânica (MO), proteína bruta (PB), extrato etéreo (EE) e carboidrato não-fibroso (CNF) semelhantes. O incremento de aditivos às silagens de CIPB favoreceram a digestibilidade da MS, MO, PB, CNF, fibra em detergente neutro (FDN) e de nutrientes digestíveis totais (NDT). Silagem a base de CIPB pode ser utilizada na dieta de ruminantes com a inclusão de aditivos, especialmente quirera de milho e raspa de mandioca, que conferem qualidade nutricional superior às demais silagens avaliadas.

El género Attalea (Arecaceae) de Bolivia: Afinidades con sistemas ecológicos regionales

La documentación de las especies neotropicales de la familia Arecaceae, basada en los recientes aportes a su taxonomía y su relación con los paisajes naturales, actualiza los patrones espaciales a los cuales se adaptan en su rango de distribución. En este caso se relevan 121 registros de especímenes de las 11 especies del género Attalea de Bolivia y su relación con 30 sistemas ecológicos que aproximan su ámbito de distribución a nivel regional. Para ello se sistematizó, se verificó y corrigieron las coordenadas geográficas vs. localidades de todos los especímenes coleccionados del género Attalea con el fin de cotejarlos con los sistemas ecológicos, utilizando las herramientas del ArgGis. Seguidamente elaboramos un dendrograma (especies vs. sistemas ecológicos) utilizando el método de distancia mínima en el programa R. El análisis de la relación de las especies con los sistemas ecológicos resalta una especie que no compone al sudoeste amazónico: A. eichleri y que procede de sistemas ecológicos del Cerrado. Entre las especies de Attalea amazónicas, A. blepharopus (endémica de Bolivia) se aísla de las demás y el resto subagrupa a especies según su presencia afín en bosques y sabanas, además del subandino y aluvial, como es para A. princeps, que se encuentra en 17 sistemas (57%). Ocho especies de Attalea son comunes con Perú y 10 con Brasil. Es importante relacionar la agrupación jerárquica de las especies de Attalea con los sistemas ecológicos en función a dinámicas paisajísticas para documentar sus patrones de espacio y también para su conservación.

The documentation of the Neotropical species of the Arecaceae family, based on the recent contributions to its taxonomy and its relationship with natural landscapes, updates the spatial patterns to which they adapt in their range of distribution. In this case 121 records of specimens of the 11 species of the genus Attalea of Bolivia and their relationship with 30 ecological systems that approximate their scope of distribution at regional level are released. To this end, the geographical coordinates were systematized, verified and corrected. Localities of all the specimens collected from the genus Attalea in order to compare them with ecological systems, using the ArgGis tools. We then elaborate a dendrogram (species vs. ecological systems) using the minimum distance method in the R program. The analysis of the relation of the species with the ecological systems highlights a species that does not compose to the southwest amazon: A. eichleri and that is native to ecological systems of the Cerrado. Among the SW Amazonian Attalea species, A. blepharopus (endemic to Bolivia) is isolated from the others and the rest subgroup species according to their presence in forests and savannas, in addition to the subandean and alluvial, as it is for A. princeps, which is found in 17 systems (57%). Eight species of Attalea are common with Peru and 10 with Brazil. It is important to relate the hierarchical grouping of the Attalea species with ecological systems in function of landscape dynamics to document their space patterns and also for their conservation.

Hormonal profile and the role of cell expansion in the germination control of Cerrado biome palm seeds.

Little information is currently available concerning the mechanisms controlling palm seed germination. We compared the anatomical and physiological aspects of seeds of two neotropical palm species showing different levels of dormancy. The seeds of Attalea vitrivir and Butia capitata were evaluated for the endogenous contents of hormones (ABA, GAs, CKs, BRs, IAA, JA, SA and the ethylene precursor ACC) in their cotyledonary petiole and operculum (structures involved in germination control), the force necessary to displace the operculum, endo-ß-mannanase activities, and embryo cell elongation. The analyses were carried out on with intact dry and imbibed seeds as well as with seeds with the operculum mechanically removed, 2, 5 and 10 days after sowing. The germinabilities of the intact seeds of A. vitrivir and B. capitata were 68% and 3%, respectively; the removal of the operculum increased germination to more than 90% in both species. Reductions of ABA and increases in GAs contents coincided with cell elongation, although there is no evidence that hormonal balance and endo-ß-mannanase activity are involved in operculum weakening. The ratio between the embryo length and the force required for operculum displacement (EL/OF) was found to be 1.9 times greater in A. vitrivir than in B. capitata, which means that very small elongations in each cell would be sufficient to promote germination, resulting in a lower level of dormancy in the former species. EL/OF and cell growth control are therefore important for defining dormancy level in palm seeds.

Life history traits influence the strength of distance- and density-dependence at different life stages of two Amazonian palms.

Background and Aims: Natural enemies are known to be important in regulating plant populations and contributing to species coexistence (Janzen-Connell effects). The strength of Janzen-Connell effects (both distance- and density-effects) varies across species, but the life history traits that may mediate such a variation are not well understood. This study examined Janzen-Connell effects across the life stages (seed through adult stages) of two sympatric palm species with distinct phenologies and shade tolerances, two traits that may mediate the strength and timing of Janzen-Connell effects. Methods: Populations of two common palm species, Attalea phalerata and Astrocaryum murumuru , were studied in Manu National Park, Peru. Seed predation experiments were conducted to assess Janzen-Connell effects at the seed stage. In the post-seed stages, spatial point pattern analyses of the distributions of individuals and biomass were used to infer the strength of distance- and density-effects. Key Results: Seed predation was both negative distance- and density-dependent consistent with the Janzen-Connell effects. However, only seedling recruitment for asynchronously fruiting Attalea phalerata was depressed near adults while recruitment remained high for synchronously fruiting Astrocaryum murumuru , consistent with weak distance-effects. Negative density-effects were strong in the early stages for shade-intolerant Attalea phalerata but weak or absent in shade-tolerant Astrocaryum murumuru. Conclusions: Distance- and density-effects varied among the life stages of the two palm species in a manner that corresponded to their contrasting phenology and shade tolerance. Generalizing such connections across many species would provide a route to understanding how trait-mediated Janzen-Connell effects scale up to whole communities of species.

Cascading effects of defaunation on the coexistence of two specialized insect seed predators.

Identification of the mechanisms enabling stable coexistence of species with similar resource requirements is a central challenge in ecology. Such coexistence can be facilitated by species at higher trophic levels through complex multi-trophic interactions, a mechanism that could be compromised by ongoing defaunation. We investigated cascading effects of defaunation on Pachymerus cardo and Speciomerus giganteus, the specialized insect seed predators of the Neotropical palm Attalea butyracea, testing the hypothesis that vertebrate frugivores and granivores facilitate their coexistence. Laboratory experiments showed that the two seed parasitoid species differed strongly in their reproductive ecology. Pachymerus produced many small eggs that it deposited exclusively on the fruit exocarp (exterior). Speciomerus produced few large eggs that it deposited exclusively on the endocarp, which is normally exposed only after a vertebrate handles the fruit. When eggs of the two species were deposited on the same fruit, Pachymerus triumphed only when it had a long head start, and the loser always succumbed to intraguild predation. We collected field data on the fates of 6569 Attalea seeds across sites in central Panama with contrasting degrees of defaunation and wide variation in the abundance of vertebrate frugivores and granivores. Speciomerus dominated where vertebrate communities were intact, whereas Pachymerus dominated in defaunated sites. Variation in the relative abundance of Speciomerus across all 84 sampling sites was strongly positively related to the proportion of seeds attacked by rodents, an indicator of local vertebrate abundance. SYNTHESIS: We show that two species of insect seed predators relying on the same host plant species are niche differentiated in their reproductive strategies such that one species has the advantage when fruits are handled promptly by vertebrates and the other when they are not. Defaunation disrupts this mediating influence of vertebrates and strongly favours one species at the expense of the other, providing a case study of the cascading effects of defaunation and its potential to disrupt coexistence of non-target species, including the hyperdiverse phytophagous insects of tropical forests.

Agronomic, morphogenic and structural characteristics of Marandu grass in silvopastoral systems composed of babassu palm and grass monoculture / Características agronômicas, morfogênicas e estruturais do capim-Marandu em sistemas silvipastoris formados por palmeiras de babaçu e em monocultura

This study evaluated the agronomic, morphogenic and structural characteristics of palisadegrass (Urochloa brizantha) in silvopastoral systems (SSPs) composed of babassu palms (Attalea speciosa) and grass monoculture in the Pre-Amazon region of the state of Maranhão, Brazil. The study followed a completely randomized design, with the arrangement in split plots with six replicates for the evaluation of agronomic characteristics and 30 repetitions for the morphogenic and structural characteristics. The plots were divided into pasture environments with different palm densities (monoculture, 80, 131, 160 palms.ha-¹), and the subplots were divided into the different seasons (rainy and dry). Total forage production was affected (P 0.05) by the interaction between palm density and season. The rainy season, compared to the dry season, presented higher total forage production, whereas among silvopastoral systems, the one with 80 palms.ha-¹ presented higher production. The same pattern was found for leaf production. In the rainy season the production of culms increased, as did the ratio leaf.culm-1. Regarding the accumulation of dead material, the only observed difference was a smaller accumulation in the dry season for pastures with 160 palms.ha-¹. In the rainy season, the leaf elongation rate, leaf appearance rate, and final leaf length had higher values in the silvopastoral syste

Objetivou-se avaliar as características agronômicas, morfogênicas e estruturais do capim-Marandu (Urochloa brizantha) em sistemas silvipastoris (SSPs) compostos por palmeiras de babaçu (Attalea speciosa) e em monocultivo de capim-Marandu na região Pré-Amazônica Maranhense. O delineamento experimental adotado foi o inteiramente casualizado, com o arranjo em parcelas subdivididas com seis repetições para a avaliação das características agronômicas e 30 repetições para as características morfogênicas e estruturais. As parcelas foram constituídas pelos ambientes pastoris e suas densidades (monocultura, 80, 131, 160 palmeiras.ha-¹) e nas subparcelas os períodos (chuvoso e seco). A produção total de forragem foi influenciada (P 0,05) pela interação densidades x épocas do ano. Entre as épocas, o período chuvoso foi o que apresentou maior produção total de forragem e entre os ambientes pastoris a densidade de 80 palmeiras.ha-¹ apresentou maior produção. A mesma resposta foi encontrada para a produção de folhas. No período chuvoso foi observado maior produção de colmos e relação folha. colmo-1. Para o acúmulo de material morto só houve efeito (P 0,05) entre os períodos, onde pastagens com 160 palmeiras.ha-¹, no período seco tiveram menor acúmulo. No período chuvoso, as taxas de alongamento foliar, aparecimento foliar, e comprimento final da folha tiveram maiores valores no am