Establishment of PCR Reaction Condition for Highly Successful Ancient DNA PCR / 체질인류학회지

The ancient bone DNA analysis essentially requires PCR amplification of the targeting genes of study due to the limitation of the ancient bone sample and DNA amounts. In contrast to the fresh living human DNA, it is common to face failing in amplifying the poorly preserved ancient DNA after death. Therefore, the optimized PCR methods appropriate for ancient DNA are required. However, there is no report to date that a systemic investigation of enhanced PCR amplification methods suitable for ancient samples has been conducted Approximately 500~3,300-year-old Korean and Mongolian ancient bones that are resistant to PCR were selected and an extensive number of PCR conditions were systematically investigated for the comparison of PCR success rates. For the PCR analysis, a mitochondrial DNA fragment as a multicopy DNA and a M175 Y chromosome biallelic marker DNA fragment as a single copy DNA that is the marker of the prevalent Y haplogroup (haplogroup O) in Korea were targeted. The identity of the amplified products were confirmed by DNA sequencing. Through this study, we established the optimized PCR conditions for the highly successful amplification of ancient bone DNAs. This estabilished method allowed for the successful amplification of mitochondrial DNAs from all the ancient bone samples tested and the amplification by 50% success rates in the amplification of M175 Y chromosome biallelic marker DNA but with the highest success rates. These results demonstrate that the optimized PCR condition will be useful for the promising ancient DNA analysis in the fields of molecular genetic anthropological studies.

Haplogroup diversity of 8 biallelic markers on human Y chromosome in Wuhan Han population / 中国法医学杂志

Objective To screen polymorphic biallelic markers on human Y chromosome in Han population , calculations of their allele and haplogroup frequency distributions to provide data for forensic application and population evolution studies. Methods Genotyping of 8 biallelic markers on human Y chromosome (M9, M89, M111, Ml19, M122, M134, IMS-JST0033050 and SRY +465) were carried out in a sample of 160 unrelated Chinese male individuals living in Wuhan using fragment length discrepant allele specific PCR (FLDAS-PCR) and PAGE technique. Results Genetic polymorphism were identified for all 8 biallelic markers in Wuhan Han population. Gene Diversity (GD) ranges from 0.0126 to 0.4830. A total of 9 different haplogroups(Hg 1-9)were observed and the haplogroup diversity (HD) were 0.7776. Conclusion The haplogroups formed by 8 biallelic markers are highly polymorphic, and can be used in conjunction with Y-STRs in forensic medicine and population evolution studies.