Stimulatory effects of smoke solution and biogas digestate slurry application on photosynthesis, growth, and methylation profiling of solanum tuberosum.

Biostimulants are obtained from various sources like plants, animals, microorganisms, and industrial by-products as well as waste material. Their utilization in agriculture practices is being increased that is giving positive results. The purpose of the current study was to use plant-derived smoke (SMK) solution and biogas digestate (BGD) slurry as biostimulant to elucidate their impact on potato (Solanum tuberosum) performance. The experiment was conducted in lab as well as field conditions, and SMK and BGD solutions were prepared in varying concentrations such as SMK 1:500, SMK 1:250, BGD 50:50, and BGD 75:25. Foliar applications were performed thrice during experiments and data were collected related to photosynthesis, growth, pigments, and genome-wide methylation profiling. Net photosynthesis rate (A) and water use efficiency (WUE) were found higher in SMK- and BGD-treated lab and field grown plants. Among pigments, BGD-treated plants depicted higher levels of Chl a and Chl b while SMK-treated plants showed higher carotenoid levels. Alongside, enhancement in growth-related parameters like leaf number and dry weight was also observed in both lab- and field-treated plants. Furthermore, DNA methylation profile of SMK- and BGD-treated plants depicted variation compared to control. DNA methylation events increased in all the treatments compared to control except for SMK 1:500. These results indicate that smoke and slurry both act as efficient biostimulants which result in better performance of plants. Biostimulants also affected the genome-wide DNA methylation profile that resultantly might have changed the plant gene expression profiling and played its role in plant responsiveness to these biostimulants. However, there is need to elucidate a possible synergistic effect of SMK and BGD on plant growth along with gene expression profiling.

The effect of anaerobic digestate as an organic soil fertilizer on the diversity and structure of the indigenous soil microbial and nematode communities.

Anaerobic digestate is a popular soil additive which can promote sustainability and transition toward a circular economy. This study addresses how anaerobic digestate modifies soil health when combined with a common chemical fertilizer. Attention was given to soil microbes and, a neglected but of paramount importance soil taxonomic group, soil nematodes. A mesocosm experiment was set up in order to assess the soil's microbial and nematode community. The results demonstrated that the microbial diversity was not affected by the different fertilization regimes, although species richness increased after digestate and mixed fertilization. The composition and abundance of nematode community did not respond to any treatment. Mixed fertilization notably increased potassium (K) and boron (B) levels, while nitrate (NO3-) levels were uniformly elevated across fertilized soils, despite variations in nitrogen input. Network analysis revealed that chemical fertilization led to a densely interconnected network with mainly mutualistic relationships which could cause ecosystem disruption, while digestate application formed a more complex community based on bacterial interactions. However, the combination of both orchestrated a more balanced and less complex community structure, which is more resilient to random disturbances, but on the downside, it is more likely to collapse under targeted perturbations.

Low uptake of pharmaceuticals in edible mushrooms grown in polluted biogas digestate.

The uptake dynamics of two sulfonamide antibiotics, two fluoroquinolone antibiotics, and the anticonvulsant carbamazepine during the cultivation of two species of edible mushrooms (Agaricus subrufescens and A. bisporus) was investigated. None of the antibiotics were accumulated by the mushrooms, while carbamazepine and its transformation product carbamazepine-10,11-epoxide were taken up by A. bisporus fruiting body but only in small amounts (up to 0.76 and 1.85 µg kg-1 dry weight, respectively). The sulfonamides were quickly removed from the mushroom growth substrate, while the recalcitrant fluoroquinolones and carbamazepine were only partially removed. Dissipation half-lives were generally lower for A. subrufescens than A. bisporus, but A. subrufescens was also grown at a slightly higher culture temperature. A. subrufescens also showed a lower uptake of contaminants. Comparison of maximum dietary intake with other common exposure sources showed that these mushrooms can safely be eaten although produced on a polluted substrate, with respect to the investigated compounds.

Recent innovations in fertilization with treated digestate from food waste to recover nutrients for arid agricultural fields.

This study aims to explore the development of sustainable fertilizers from waste materials of a biogas plant and a brewery. These wastes, rich in organic carbon and nitrogen, were processed with sulfuric(VI) and phosphoric(V) acid mixture, facilitating the production of free amino acids and achieving waste sanitization. This treatment produced by-products, which extended the range of possible applications. The highest concentration of free amino acids (360 mg/l) was achieved through hydrolyzing with a 40% concentration medium over 24 h. In this case, the maximum levels were recorded for beta-alanine (69.3 mg/l), glycine (46.8 mg/l), isoleucine (43.5 mg/l), proline (36.2 mg/l), and valine (31.5 mg/l). The study presents two fertilizer technologies, with and without micronutrients, that satisfy European Parliament Regulation 2019/1009 (Ntot > 2%, Norg > 0.5%, Corg > 3%). Bioavailability of nutrients in the formulations ranged from 60 to 100%. The efficacies of these fertilizers were evaluated in 30-day pot trials with various plant species, with both single application and fertigation tested. Multielement analysis confirmed high nutrient transfer in the soil-plant system, and the inclusion of micronutrients led to biofortification of plant biomass in Cu (48.3 ± 7.2 mg/kg), Mn (249 ± 37 mg/kg), Zn (164 ± 25 mg/kg), and Fe (211 ± 32 mg/kg). These sustainable fertilizers present an alternative to traditional, non-renewable fertilizers and offer promising solutions for precision agriculture and environmentally conscious production.

Effect of anaerobic digestion on odor and ammonia emission from land-applied cattle manure.

High ammonia (NH3) and odor emission can occur after land application of liquid animal manure. This study was aimed at evaluating NH3 loss and odor nuisance after field application of cattle manure and how it is affected by two anaerobic digestion strategies: i) digestion of cattle manure alone and ii) digestion with catch crops and dilution by water. A system of dynamic chambers with online measurements of NH3 and odorous compounds (summarized as odor activity value, OAV) was used. Two experiments were conducted under different temperature conditions. The results demonstrated that anaerobic digestion did not affect NH3 loss but did decrease OAV. Addition of catch crops and water to the digestion process reduced both NH3 loss and OAV. Cool temperature in one of the experiments had a large effect on both NH3 and odor emissions, and at high temperature the differences between treatments increased.

Uptake of Ultrashort Chain, Emerging, and Legacy Per- and Polyfluoroalkyl Substances (PFAS) in Edible Mushrooms (Agaricus spp.) Grown in a Polluted Substrate.

Uptake of 19 per- and polyfluoroalkyl substances (PFAS), including C3-C14 perfluoroalkyl carboxylic acids (PFCAs), C4, C6, and C8 perfluoroalkyl sulfonates (PFSAs), and four emerging PFAS, was investigated in two mushroom species (Agaricus bisporus and Agaricus subrufescens) cultivated in a biogas digestate-based substrate. Accumulation of PFAS in mushrooms was low and strongly chain-length dependent. Among the different PFCAs, bioaccumulation factors (log BAFs) decreased from a maximum of -0.3 for perfluoropropanoic acid (PFPrA; C3) to a minimum of -3.1 for perfluoroheptanoate (PFHpA; C7), with only minor changes from PFHpA to perfluorotridecanoate (PFTriDA; C13). For PFSAs, log BAFs decreased from perfluorobutane sulfonate (PFBS; -2.2) to perfluorooctane sulfonate (PFOS; -3.1) while mushroom uptake was not observed for the alternatives 3H-perfluoro-3-[(3-methoxy-propoxy)propanoic acid] (ADONA) and two chlorinated polyfluoro ether sulfonates. To the best of our knowledge, this is the first investigation of the uptake of emerging and ultra-short chain PFAS in mushrooms, and generally the results indicate very low accumulation of PFAS.

Feasibility of housefly larvae-mediated vermicomposting for recycling food waste added digestate as additive.

The development of methods for the efficient treatment and application of food waste digestate is an important research goal. Vermicomposting via housefly larvae is an efficient way to reduce food waste and achieve its valorization, however, studies on the application and performance of digestate in vermicomposting are rarely. The present study aimed to investigate the feasibility of the co-treatment of food waste and digestate as an additive via larvae. Restaurant food waste (RFW) and household food waste (HFW) were selected to assess the effects of waste type on vermicomposting performance and larval quality. Waste reduction rates of 50.9%-57.8% were observed in the vermicomposting of food waste mixed with digestate at a ratio of 25%, which were slightly lower than those for treatments without the addition of digestate (62.8%-65.9%). The addition of digestate increased the germination index, with a maximum value of 82% in the RFW treatments with 25% digestate, and decreased the respiration activity, with a minimum value of 30 mg-O2/g-TS. The larval productivity of 13.9% in the RFW treatment system with a digestate rate of 25% was lower that without digestate (19.5%). Materials balance shows that larval biomass and metabolic equivalent had decreasing trends as the amount of digestate increased and HFW vermicomposting exhibited lower bioconversion efficiency than that of RFW treatment system regardless of the addition of digestate. These results suggest that mixing digestate at a low ratio (25%) during vermicomposting of food waste especially RFW could lead to considerable larval biomass and generate relatively stable residues.

Acidification and alkalinization pretreatments of biowastes and their effect on P solubility and dynamics when placed in soil.

BACKGROUND: Sustainability concerns as well as recent increases in fertilizer prices exacerbates the need to optimise the use of biowastes as fertilizers. For this reason, we investigated how different pretreatments affect the P dynamics when biofertilizers are placed in the soil. METHODS: Sewage sludge (SS), sewage sludge ash (SS-ash), meat and bone meal (MBM), and the solid fraction of biogas digestate (BGF) were pretreated with H2SO4, NaOH, and Ca(OH)2 and incubated for 2 and 12 days, respectively, in a one-dimensional reaction system for detailed studies of the interactions in the biomaterial-soil interface and the soil adjacent to the placement zone. RESULTS: Our results showed that acidification and treatment with NaOH increased the P solubility of the biomaterials. The P loss from the biomaterial layer to the soil was correlated with water-extractable P in the biomaterials (0.659) and water-extractable P in the soil (0.809). Acidification significantly increased the total amount of P depleted from the biomaterial to the soil whereas NaOH pre-treatment did not. However, for NaOH-treated SS and SS-ash, the apparent recoveries were significantly higher compared to the acidification due to a decrease in soil P sorption capacity as the soil pH increased due to residual alkalinity in the biomaterials. CONCLUSIONS: Acidification showed promising results by increasing the P solubility of all the biomaterials, and the alkalinization of SS and SS-ash with NaOH by increasing the apparent recovery in the soil. However, further studies are needed to assess the effects of these treatments on plant growth and P uptake.

Field assessment of organic amendments and spring barley to phytomanage a Cu/PAH-contaminated soil.

The INTENSE project, supported by the EU Era-Net Facce Surplus, aimed at increasing crop production on marginal land, including those with contaminated soils. A field trial was set up at a former wood preservation site to phytomanage a Cu/PAH-contaminated sandy soil. The novelty was to assess the influence of five organic amendments differing in their composition and production process, i.e. solid fractions before and after biodigestion of pig manure, compost and compost pellets (produced from spent mushroom substrate, biogas digestate and straw), and greenwaste compost, on Cu availability, soil properties, nutrient supply, and plant growth. Organic amendments were incorporated into the soil at 2.3% and 5% soil w/w. Total soil Cu varied from 179 to 1520 mg kg-1, and 1 M NH4NO3-extractable soil Cu ranged from 4.7 to 104 mg kg-1 across the 25 plots. Spring barley (Hordeum vulgare cv. Ella) was cultivated in plots. Changes in physico-chemical soil properties, shoot DW yield, shoot ionome, and shoot Cu uptake depending on extractable soil Cu and the soil treatments are reported. Shoot Cu concentration varied from 45 ± 24 to 140 ± 193 mg kg DW-1 and generally increased with extractable soil Cu. Shoot DW yield, shoot Cu concentration, and shoot Cu uptake of barley plants did not significantly differ across the soil treatments in year 1. Based on soil and plant parameters, the effects of the compost and pig manure treatments were globally discriminated from those of the untreated, greenwaste compost and digested pig manure treatments. Compost and its pellets at the 5% addition rate promoted soil functions related to primary production, water purification, and soil fertility, and the soil quality index.

Effectiveness of cattle dung biogas digestate on spinach growth and nutrient uptake.

New farming techniques should be introduced to improve yield quality and quantity while taking preservation of the environment into consideration. This study investigated effectiveness of cattle dung biogas digestate on spinach growth and nutrient uptake. Spinach was grown with cattle dung biogas digestate (BD), inorganic fertiliser (IF) and unfertilised control (CO) treatments under complete randomised design field conditions. Spinach planted under BD showed significantly higher growth in terms of plant height and number of leaves compared to spinach under CO and IF. A linear relationship between leaf numbers and leaf area index (LAI) (R2 0.691, p < 0.0001) was established for the study. Cattle dung biogas digestate (BD) plants produced significantly the longest roots followed by IF plants. The IF plants produced more biomass per rooting depth (0.85 g cm-1) than BD (0.61 g cm-1) and CO (0.35 g cm-1). Regarding macronutrient content of the spinach leaves, significant differences were only observed for potassium (K) in the order of IF (8.6 g kg-1), BD (6.8 g kg-1), and CO (6.7 g kg-1). Significantly higher amounts of zinc (Zn2+) accumulated in spinach leaves under BD compared to IF and CO. Fertilising spinach with BD improves growth and development just as much as IF. Additional benefits include improving nutrient content of the spinach, assisting with environmental preservation and decreasing production cost.

Selecting the optimal nutrients recovery application for a biogas slurry based on its characteristics and the local environmental conditions: A critical review.

Digestate (effluent of biogas plants) became the main bottleneck for biogas industry expansion because it often exceeds the capacity of surrounding croplands as fertilizer. Nutrients recovery from digestate is a promising solution for closing nutrients cycles and generating high value-added byproducts. In fact, numerous nutrients recovery technologies were reported and utilized for that purpose. However, each technology has optimum working conditions, while digestates have different characteristics due to the different substrates, digestion conditions, and handling methods. On the other hand, no protocol has been reported yet for selecting the optimal nutrients recovery technology or sequenced technologies for different digestates regarding their characteristics and the surrounding environmental conditions. In this study, an interactive flowchart was suggested and discussed for selecting the most appropriate technology or sequential techniques among the different alternatives. The whole digestate utilization technologies, solid-liquid separation technologies, liquid and solid processing technologies were included.

Tunable active sites on biogas digestate derived biochar for sulfanilamide degradation by peroxymonosulfate activation.

Conversion of digestate into biochar-based catalysts is an effective strategy for disposal and resource utilization. The active sites on biochar correlated with reactive species formation in peroxymonosulfate (PMS) system directly. Clarifying the structure-performance relationship of digestate derived biochar in PMS system was essential for decomposition of contaminants. Herein, dairy manure digestate derived biochar (DMDB) was prepared for PMS activation and sulfamethoxazole (SMX) degradation. The higher pyrolysis temperature could promote effective sites generation. Especially, the DMDB-800 catalyst exhibited excellent performance for PMS activation, achieving 90.2% degradation of SMX within 60 min. Based on the correlation analysis between log (k) values and active sites, defects, graphite N and CO were identified as dominant sites for PMS activation. The 1O2 oxidation and surface electron transfer were critical routes for SMX degradation. Besides, the degradation pathways of SMX were proposed according to DFT calculations and intermediates determination. The cleavage of the sulfonamide bond, hydroxylation of the benzene ring and oxidation of the amino group mainly occurred during SMX degradation. Overall, this study provides deep insights into the enhanced mechanism of tunable active sites on DMDBs for PMS activation, boosting the application of digestate biochar for water treatment in advanced oxidation systems.

Evaluation of the effect of different concentrations of organic amendments and botanical extracts on the mortality and hatching of Meloidogyne javanica.

Organic amendments and botanical extracts are considered as some of the eco-friendly alternatives to chemical pesticide in suppressing plant pathogenic nematodes (PPN). Root-knot nematode (RKN) is the most important group of PPN distributed globally causing both qualitative and quantitative damage to many crops. Vermicompost and biogas digestate (BD) are two forms of organic amendments reported to have potential to limit RKN infestation. Likewise, marigold (Tagates spp.) and cabbage (Brassica oleracea) are two widely studied botanicals having shown their potential to control RKN. However, there was not much in vitro research related to organic amendments and botanicals targeting a particular species of RKN to observe their nematicidal effect. An in vitro experiment was undertaken to evaluate the effect of these organic amendments and botanical extracts at different concentrations (10.0%, 25.0%, 50.0% and 100.0%) on the hatching and mortality of Meloidogyne javanica at different time spans. Mortality of J2 and inhibition of hatching of egg mass of M. javanica differed significantly (p < 0.0001) among the interaction effect of treatments and incubation time for both organic amendments and botanical extracts. Findings of this experiment indicated that potentiality for increasing mortality and inhibition of hatching was higher and steadier in botanical extracts than those of organic amendments.

Organic Waste-Based Fertilizer in Hydroponics Increases Tomato Fruit Size but Reduces Fruit Quality.

In regions with intensive agricultural production, large amounts of organic waste are produced by livestock animals. Liquid digestate from manure-based biogas production could potentially serve as fertilizer if integrated with closed horticultural irrigation systems. The aim of this experiment was to investigate how fertilizer based on liquid biogas by-products of pig manure digestion can affect the growth and production of tomato plants. Integration of a nitrification bioreactor presumes a significantly lower concentration of nutrient solutions and a higher level of oxygenation than classical mineral cultivation. Therefore, additional controls were included. We compared plant growth and fruit quality traits of tomato plants grown in a hydroponic solution with organic fertilizer with two levels of mineral fertilizer. The tomatoes grown with organic waste-based liquid fertilizer showed reduced growth rates but increased mean fruit size, resulting in no significant change in total yield compared with high-mineral cultivation. The growth rate was similarly reduced in plants cultivated with low-mineral fertilizer. Plants cultivated with organic waste-based fertilizer had high Cl- concentration in xylem sap, leaves, and, ultimately, fruits. The leaves of plants cultivated with organic waste-based fertilizer contained higher concentrations of starch and soluble carbohydrate and low concentrations of phosphorous (P) and sulfur (S). The plants grown with organic waste-based or low-mineral medium showed significantly poorer fruit quality than the plants cultivated with the high-mineral solution. The low-mineral treatment increased xylem sap contribution to fruit weight because of higher root power. The organic waste-based fertilization did not change the root power but increased fruit size. In conclusion, organic waste-based cultivation is a possible solution for sustainable plant production in greenhouses. However, additional adjustment of nutrient supply is required to improve fruit quality.

Pretreatment by composting increased the utilization proportion of pig manure biogas digestate and improved the seedling substrate quality.

Anaerobic digestion of organic residues has the potential to significantly contribute to a shift from fossil to renewable energy, while the remaining biogas digestate need to be treated or used for a second time. In this study, the pig manure biogas digestate (PMBD) was evaluated as a potential part of seedling substrates and composting was considered a pretreating method to improve its characters. Composting was carried out firstly in a forced aeration composting system (100 L), in which perlite and sawdust were used as additives in different proportions separately or together. Based on the comparison of the physicochemical characters of different seedling substrates formulas mixed with PMBD or pig manure biogas digestate pretreated by composting (CPMBD), selected seedling substrates were analyzed by bioassay experiment. The results showed that pretreatment by composting and the additives (perlite and sawdust) used in composting decreased the pH value of PMBD and make it suitable for seedling substrates, especially composted with perlite and sawdust. Both PMBD and CPMBD with low proportion in the substrates improved plant growth of lettuce and tomato, while CPMBD was better than PMBD. However, when the proportions of PMBD was increased more than 20% and CPMBD was increased more than 40%, plant growth inhibition was observed. Tomato was more sensitive than lettuce to the physicochemical characters of the substrate. In summary, PMBD pretreated by composting not only increases the uses of digestate, but also enhances plant growth and hence yield.

Effect of residence time during hydrothermal carbonization of biogas digestate on the combustion characteristics of hydrochar and the biogas production of process water.

The biogas digestate from anaerobic digestion of cow manure and energy crops was treated by hydrothermal carbonization (HTC) at 210 °C for 0.5 to 5 h to understand the effect of HTC residence time on the combustion characteristics of hydrochar and the biogas production of process water. The increase in HTC residence time slightly reduced the higher heating values (16.3-16.0 MJ/kg) but improved most slagging and fouling indices of the hydrochar. However, the slagging and fouling during hydrochar combustion were almost impossible to avoid. The specific methane yield of the process water was not significantly influenced by the HTC residence time. Energy assessment demonstrated that HTC for 0.5 h achieved the highest process efficiency and net energy gain when the combustion energy was obtained from hydrochar and CH4 (from process water). Therefore, the HTC condition of 210 °C, 0.5 h is suggested to valorize biogas digestate for energy production.

Dispersion of Antibiotic Resistance Genes (ARGs) from stored swine manure biogas digestate to the atmosphere.

Biogas digestate is a reservoir of antibiotic resistance genes (ARGs) and could pose a high health risk to both human and animals if the host microorganisms of ARGs become aerosolized. The purpose of this study was to investigate the diversity and relative abundance characteristics of aerosol-loaded ARGs from biogas digestate during storage, and to explore whether the change of ARGs in biogas digestate directly affect the dispersion of aerosol-loaded ARGs. This study reported for the first time that 28 of 42 ARG subtypes detected in the biogas digestate could be dispersed to the atmosphere via aerosol dispersion in a lab-scale dynamic emission vessels experiment. The relative abundance or diversity of ARGs in aerosols were different from that in biogas digestate, and no significant correlation were observed between the relative abundance of ARGs in biogas digestate and aerosols. The dominant ARGs were tetracycline resistance genes in biogas digestate and ß-lactam resistance genes in aerosols. The process of biogas digestate storage reduced the total relative abundance of targeted ARGs in biogas digestate, decreased by 0.35 copies/16S rRNA after 30 days of storage, but increased the abundance of some ARG subtypes, including tetM, tetX, tetQ, tetS, ermF and sul2. High-concerned ARGs, including NDM-1, mcr-1 and vancomycin resistance genes (including vanA, vanB, vanRA and vanSA), were found in biogas digestate, and NDM-1 and vanB were also detected in aerosols. These results indicated a potential risk of ARGs dispersion during biogas digestate storage. Further research on the dispersion of ARGs from biogas digestate is required to elucidate the emission mechanism and develop mitigation measures.

Effect of Pretreatment on Hydraulic Performance of the Integrated Membrane Process for Concentrating Nutrient in Biogas Digestate from Swine Manure.

Nanofiltration (NF) or reverse osmosis (RO) process has been widely applied for concentrating nutrient in biogas digestate. However, efficient pretreatment is key to the sustainable operation of NF or RO. In this study, the combination of NF and RO for concentrating biogas digestate was compared using different pretreatments of hollow fiber ultrafiltration membrane (HFUFM) and ceramic membrane (CUFM). Pilot-scale batch tests were conducted (500 L). CUFM showed a higher membrane flux than HFUFM (100 ~ 180 L·(m2·h)-1 vs. 17 ~ 35 L·(m2·h)-1), but they showed little impact on the NF + RO process. Membrane fluxes of NF and RO were 20 ~ 48 L·(m2·h)-1 and 16 ~ 40 L·(m2·h)-1, respectively. In the RO permeates, the removal rates of total suspended solids (TSS), total solids (TS), chemical oxygen demand (COD), total nitrogen (TN), NH4+-N, and Cl- were above 91%. In the concentrates, TN and total potassium (TK) were concentrated by 1.60 and 2.00 folds in the NF stage, and by 2.10 and 2.30 folds in the RO stage. Further attention should be paid to the antibiotics risks in the concentrates before they are utilized as plant fertilizers.

Isolation of antibiotic-resistant bacteria in biogas digestate and their susceptibility to antibiotics.

Antibiotics are widely used to prevent and treat diseases and promote animal growth in the livestock industry, and therefore antibiotic residues can end up in biogas digestate from processes treating animal manure (AM) and food waste (FW). These digestates represent a potential source of spread of antimicrobial resistance (AMR) when used as fertilisers. This study evaluated AMR risks associated with biogas digestates from two processes, using AM and FW as substrate, by isolation and identification of antibiotic-resistant bacteria (ARB) and testing their susceptibility to different antibiotics. ARB from the digestates were isolated by selective plating. The antibiotic susceptibility profile of isolates was determined using ampicillin, ceftazidime, meropenem, vancomycin, ciprofloxacin, rifampicin, chloramphenicol, clindamycin, erythromycin, tetracycline, gentamicin or sulfamethoxazole/trimethoprim, representing different antibiotic classes with differing mechanisms of action. In total, 30 different bacterial species belonging to seven genera were isolated and classified. Bacillus and closely related genera, including Paenibacillus, Lysinibacillus and Brevibacillus, were the dominant ARB in both digestates. Most of the ARB strains isolated were non-pathogenic and some were even known to be beneficial to plant growth. However, some were potentially pathogenic, such as an isolate identified as Bacillus cereus. Many of the isolated species showed multi resistance and the AM digestate and FW digestate both contain bacterial species resistant to all antibiotics tested here, except gentamicin. A higher level of resistance was displayed by the FW isolates, which may indicate higher antibiotic pressure in FW compared with AM digestate. Overall, the results indicate a risk of AMR spread when these digestates are used as fertiliser. However, most of the ARB identified are species commonly found in soil, where AMR in many cases is abundant already, so the contribution of digestate-based fertiliser to the spread of AMR may still be very limited.

Comparative nutrient leaching capability of cattle dung biogas digestate and inorganic fertilizer under spinach cropping condition.

A field experiment was used to determine leaching capability of biogas digestate used as soil amendment in comparison to inorganic fertilizer under spinach cropping condition. The biogas digestate used in this experiment was obtained from a biogas production plant that used cattle dung as feedstock. Spinach was cultivated under three treatments, namely, biogas digestate (BD), inorganic fertilizer (IN) and control (no fertilizer or biogas digestate). All the treatments were replicated three times. Irrometer soil solution access tubes were inserted in the middle of each plot for the collection of the nutrient leachates. The leaf area of spinach was significantly higher in IN treatment than BD and control at the harvest stage; BD produced higher fresh mass (479 g/plant) than IN (468 g/plant) and control (201 g/plant). The leachates of inorganic fertilizer showed higher nutrient concentration compared to biogas digestate and control treatments. Twenty-eight days after transplanting, the leachate consisted of 3670.7, 12.12 and 8.5 mg/l of ammonium and 408, 83 and 39 mg/l of phosphate at IN, BD and control treatments, respectively. The study demonstrates that cattle dung biogas digestate can be applied on soil as fertilizer for crops with little or no environmental consequences to water resources and still have the same product quality as inorganic fertilizer.