RESUMO
To determine the effect of Qingchang Oral Liquid (QOL) on second generation merozoite of chicken E. tenella, healthy Roman pink chickens were randomly divided into model group and QOL group (drug group), and both groups of chicks were inoculated with 5×104 sporulated oocysts by oral gavage. Then, the drug group was given QOL at a dose of 2.4 ml/kg, and the model group was given the same volume of normal saline. After 120 hours of inoculation, both groups of experimental chickens were killed at the same time, their caecum tissues were collected, the second generation merozoite were separated, the ultra-microstructure of the second generation merozoite were observed with transmission electron microscope and the mitochondrial membrane potential and apoptosis proportion of the second generation merozoite were analyzed with flow cytometer. The current results suggested that QOL could cause swelling and vacuoles of mitochondria, swelling of endoplasmic reticulum and damage of outer membrane in the second generation merozoite of E. tenella. Compared with the model group, the drug group could increase the total apoptosis rate of the second generation merozoite (p<0.01), and reduce the depolarization rate of mitochondrial membrane potential (p<0.01). Conclusion: QOL can directly affect the outer membrane and mitochondria of the second generation merozoite of E. tenella, reduce the depolarization rate of mitochondrial membrane potential of the second generation merozoite and increase the apoptosis rate of the second generation merozoite.
Assuntos
Feminino , Animais , Apoptose , Galinhas/crescimento & desenvolvimento , Galinhas/fisiologia , MerozoítosRESUMO
To determine the effect of Qingchang Oral Liquid (QOL) on second generation merozoite of chicken E. tenella, healthy Roman pink chickens were randomly divided into model group and QOL group (drug group), and both groups of chicks were inoculated with 5×104 sporulated oocysts by oral gavage. Then, the drug group was given QOL at a dose of 2.4 ml/kg, and the model group was given the same volume of normal saline. After 120 hours of inoculation, both groups of experimental chickens were killed at the same time, their caecum tissues were collected, the second generation merozoite were separated, the ultra-microstructure of the second generation merozoite were observed with transmission electron microscope and the mitochondrial membrane potential and apoptosis proportion of the second generation merozoite were analyzed with flow cytometer. The current results suggested that QOL could cause swelling and vacuoles of mitochondria, swelling of endoplasmic reticulum and damage of outer membrane in the second generation merozoite of E. tenella. Compared with the model group, the drug group could increase the total apoptosis rate of the second generation merozoite (p<0.01), and reduce the depolarization rate of mitochondrial membrane potential (p<0.01). Conclusion: QOL can directly affect the outer membrane and mitochondria of the second generation merozoite of E. tenella, reduce the depolarization rate of mitochondrial membrane potential of the second generation merozoite and increase the apoptosis rate of the second generation merozoite.(AU)
Assuntos
Animais , Feminino , Galinhas/crescimento & desenvolvimento , Galinhas/fisiologia , Merozoítos , ApoptoseRESUMO
La medicina tradicional china se expandió por Europa en los siglos XVI y XVII a través de las crónicas de los misioneros. Entra a la isla de Cuba amparada por la Orden Real del 7 de julio de 1847, que legalizaba el uso de braceros asiáticos en Cuba. En 1854 llega Chang Pon Piang a La Habana a bordo de un bergantín de emigrantes chinos culíes. Incluía en su terapéutica medicamentos preparados a partir de plantas medicinales cubanas o productos extranjeros importados que compraba en cualquiera de los establecimientos de la Capital. Acusado de ejercicio ilegal de la medicina se traslada en el año 1871 a Cárdenas, donde ejerce su profesión hasta su enigmática muerte(AU)
Chinese traditional medicine spread out over Europe in the XVI and XVII centuries through the missionaries chronicles. It entered the Island of Cuba protected by the Royal Order of July 7th, 1847, legalizing the use of Asiatic laborers in Cuba. Chang Pon Piang arrives to Havana in 1854 on-board of a brig of Chinese emigrant-laborers. He included in his therapy medicines prepared using Cuban medicinal plants or foreign imported products he used to buy in stores of the Capital. Accused of illegally practicing medicine, he moved to Cardenas where he practiced his profession up to his enigmatic death(AU)