The Effect of Opuntia ficus Mucilage Pectin and Citrus aurantium Extract Added to a Food Matrix on the Gut Microbiota of Lean Humans and Humans with Obesity.

Experimental studies have provided evidence that physicochemical interactions in the food matrix can modify the biologically beneficial effects of bioactive compounds, including their effect on gut microbiota. This work aimed to evaluate the effect of a food gel matrix with Opuntia ficus cladodes mucilage pectin and Citrus Aurantium extract on the growth of four beneficial gut bacteria obtained from the fecal microbiota of people who are lean or who have obesity after digestion in the upper digestive system. To accomplish this, a base formulation of Opuntia ficus cladodes mucilage with or without C. aurantium extract was submitted to an ex vivo fecal fermentation in an automatic and robotic intestinal system. The changes in the intestinal microbiota were determined by means of plate culture and 16S sequencing, while short-chain fatty acids (SCFA) produced in the colon were determined via gas chromatography. In the presence of the extract in formulation, greater growth of Bifidobacterium spp. (+1.6 Log10 Colonic Forming Unit, UFC) and Lactobacillus spp. (+2 Log10 UFC) in the microbiota of lean people was observed. Only the growth in Salmonella spp. (-1 Log10 UFC) from both microbiota was affected in the presence of the extract, which decreased in the ascending colon. SCFA was mainly produced by the microbiota of people who were lean rather than those who had obesity in the presence of the extract, particularly in the ascending colon. The effect of sour orange extract seems to depend on the origin of the microbiota, whether in people who have obesity (25 mM/L) or are lean (39 mM/L).

Physicochemical Quality and Fatty Acid Profile in the Meat of Goats Fed Forage Cactus as a Substitute for Tifton 85 Hay.

Low rainfall in Northeast Brazil is a limiting factor for animal production. Forages that present crassulacean acid metabolism, such as forage cactus, are adapted to the edaphoclimatic conditions of this region, as they lose little water through the stomata. Thus, the objective was to evaluate the physical and chemical quality, fatty acid profile and sensory acceptance of the meat from goats fed forage cactus as a substitute for Tifton 85 hay. Twenty-one uncastrated mixed-breed goats with a mean body weight of 18 ± 0.86 kg and 7 ± 1 months of age were used. A completely randomized design with three treatments and seven replications per treatment was performed. The inclusion of 0 (control), 25 and 55% of forage cactus in substitution of Tifton 85 hay in the diet of the goats was evaluated. The lipid content in the meat of animals fed 25 and 55% of forage cactus was 1.33% and 1.26%, respectively, and was lower (p < 0.05) in relation to the meat of animals that received the control diet (1.56%). The inclusion of 55% of forage cactus provided an increase (p < 0.05) in the content of monounsaturated fatty acids in the meat (52.71%) in relation to the control meat (37.75%). Sensory analysis differed (p < 0.05) between treatments. We recommend replacing Tifton 85 hay with 55% forage cactus, as it presents greater sensory acceptance, and provides lower lipid content and higher content of monounsaturated fatty acids in goat meat.

Biomass components and water use efficiency in cactus pear under different irrigation systems and harvest frequencies

The objective was to evaluate the effect of different irrigation systems and harvest frequencies on the cultivation of cactus pear. The study was conducted in the semi-arid region, in the municipality of Russas, CE, Brazil. The experiment was a split plot randomized block design, with four replications. Plots were represented by irrigation systems (surface drip, microsprinkler, Micro Spray Jet, and conventional sprinkler) and subplots by harvest frequencies (6, 9, 12, and 18 months). Cactus pear biomass production characteristics were evaluated. Most of the productive characteristics evaluated were influenced by harvest frequency and irrigation system. The harvest frequency of 12 months combined with conventional sprinkler system resulted in a total dry biomass production of 16,400 kg ha−1. The conventional sprinkler system showed the highest number of second-order cladodes (4.9 cladodes plant−1). The conventional sprinkler and Micro Spray Jet systems showed the highest values for various structural characteristics. For water use efficiency (WUE), the highest value was found at the frequency of six months (8.46 kg ha−1 mm−1). Regarding the effects of irrigation system on WUE, the highest values were observed with the conventional sprinkler system (8.20 kg ha−1 mm−1). The harvest frequency of 12 months combined with the conventional sprinkler system presents better results in the evaluated productive characteristics, mainly the cactus pear production by area.(AU)

Partially replacing sorghum silage with cactus (Opuntia stricta) cladodes in a soybean oil-supplemented diet markedly increases trans-11 18:1, cis-9, trans-11 CLA and 18:2 n-6 contents in cow milk.

Based on low 18:0 contents observed in milk fat of cows fed cactus cladodes (CC), we hypothesized that including Opuntia stricta cladodes in a soybean oil (SO)-supplemented diet would promote incomplete rumen biohydrogenation of supplemental PUFA, leading to increased trans-11 18:1 and cis-9, trans-11 CLA contents in milk. Twelve Holstein cows were used in a two-period study: (a) Baseline: all cows received a total mixed ration (TMR) composed of sorghum silage (SS) and a concentrate containing no SO for 14 days; (b) Treatment: cows received one of the following SO-supplemented diets for 21 days: (1) SS-TMR: a TMR composed of SS and a SO-enriched concentrate, (2) CC-TMR: a TMR containing CC as a partial substitute for SS plus the SO-enriched concentrate, and (3) CC-PMR: same diet as in treatment 2, but CC were mixed with the SO-enriched concentrate and fed as a partial mixed ration (PMR). Both CC diets increased relative abundances of trans-11 18:1, cis-9, trans-11 CLA, and 18:2 n-6 in milk fat, whereas opposite effects were observed on 18:0 and cis-9 18:1. Proportion of 18:2 n-6 increased, and cis-9, trans-11 CLA tended to increase with CC-PMR as compared to CC-TMR, whereas 18:3 n-3 was higher with CC-PMR than with SS-TMR. Proportions of several odd- and branched-chain fatty acids, certain 18:1 isomers, and trans-9, cis-11 CLA changed with CC diets, notably with CC-PMR. Milk yield and intake of most nutrients (except fibre) increased or tended to increase with the CC diets, whereas gross milk composition was unaltered. Stearoyl-CoA desaturase-1 index for C18 (SCD18 ) was higher with CC-PMR than with SS-TMR, and milk n-6:n-3 FA ratio and apparent transfer of 18:2 n-6 to milk increased with CC diets. These results indicate that Opuntia stricta cladodes can be a valuable feed ingredient for improving the nutraceutical value of milk fat.

In vitro biological control of bovine parasitic nematodes by Arthrobotrys cladodes, Duddingtonia flagrans and Pochonia chlamydosporia under different temperature conditions.

Variations in temperature can affect the development of nematophagous fungi, especially when they are used in the biological control of parasitic nematodes in the pastures where cattle are reared. The aim of this work was to evaluate the effects of temperature on the performance of nematophagous fungi in the biological control of bovine parasitic nematodes. The mycelial growth, chlamydospore production and nematicidal activity of Duddingtonia flagrans, Arthrobotrys cladodes and Pochonia chlamydosporia were evaluated at 15, 20, 25, 30 and 35°C. The fungal strains achieved mycelial growth, chlamydospore production and nematicidal activity on parasitic nematodes under all temperature conditions tested. The fungi showed higher growth at intermediate temperatures (20, 25 and 30°C) than at the extremes of 15 and 35°C. At 25 and 30°C, D. flagrans realized 96.8 and 94.5% nematicidal activity on bovine parasitic nematodes, respectively. Arthrobotrys cladodes effected nematicidal activity of 85.3 and 83.5%, at 20 and 25°C, respectively. At 20 and 30°C, P. chlamydosporia achieved nematicidal activity of 81.3 and 87.4%, respectively. The maximum chlamydospore production was reached at 20, 25 and 30°C for D. flagrans, at 20 and 25°C for A. cladodes and P. chlamydosporia. The results of this study demonstrated that the tested fungal strains of D. flagrans, A. cladodes and P. chlamydosporia, when used in the biological control of bovine parasitic nematodes, were not limited by in vitro temperature variations. Therefore, the use of these strains of fungi as biological control agents of parasitic nematodes is promising.

Arthrobotrys cladodes and Pochonia chlamydosporia in the biological control of nematodiosis in extensive bovine production system.

Cattle production in extensive systems favours the occurrence of gastrointestinal nematodes, and the use of nematophagous fungi complements the control strategies for these nematodes. The aim of this study was to evaluate the effectiveness of the fungi Arthrobotrys cladodes and Pochonia chlamydosporia in the biological control of gastrointestinal parasitic nematodes in grazing cattle. Twenty-four calves were randomly divided into four groups and allocated to independent paddocks from February 2018 to January 2019. In the first group, the animals received pellets containing P. chlamydosporia. In the second group, the animals received pellets containing A. cladodes. In the third group, the animals received pellets containing a combination of the fungi A. cladodes and P. chlamydosporia. In the control group, the animals received pellets without fungus. The combined use of A. cladodes and P. chlamydosporia showed greater efficacy in the biological control of bovine gastrointestinal parasitic nematodes than the same fungi used separately. The parasite load was lower and weight gain was greater (P ⩽ 0.05) in the groups of cattle treated with nematophagous fungi. Therefore, the use of A. cladodes and P. chlamydosporia is promising in the biological control of nematodiosis in cattle.

Enzymatic hydrolysis of Opuntia ficus-indica cladode by Acinetobacter pittii and alcohol fermentation by Kluyveromyces marxianus: pH, temperature and microorganism effect.

In the present work the effect of three factors: pH, temperature and type of microorganism using a factorial design 32x2 was evaluated on: growth, total cellulases hydrolytic activity (FPases), endoglucanases hydrolytic activity (CMCases), free reducing sugars (FRS), glucose, sucrose, and alcohol production using a culture medium based on mineral salts added with cladodes flour of Opuntia ficus-indica at 20% as the unique carbon source. Two wild microorganisms were used, Acinetobacter pittii a bacteria isolated from decaying cladodes, and Kluyveromyces marxianus a yeast isolated from termite stomach. The maximum hydrolytic activities were obtained with Acinetobacter pittii at 37 °C and pH 6.5 for total cellulases (0.67 ±â€¯0.02 IU/ml) and for endoglucanases (0.23 ±â€¯0.02 IU/ml) at 24 and 4 h. The maximum production of alcohol was 12.98 ±â€¯0.06 g/L obtained with Kluyveromyces marxianus at 4 h with conditions of 40 °C and pH 5.5.

Waste residues from Opuntia ficus indica for peroxidase-mediated preparation of phenolic dimeric compounds.

A methodology to detect peroxidase activity in Opuntia ficus indica cladodes waste extracts was performed and then used towards phenolic compounds. The extracts were able to dimerize three different molecules. Dimeric compounds were produced with yields ranging from 11% to 55%. The influence of H2O2 concentration was also tested, finding better yields when the peroxide-to-substrate ratio was 1:1. Some water-miscible solvents were used trying to increase overall yields, but no-significant positive results were found. In fact, one of them, THF, seemed to inhibit dimerization reaction. Hence, we have tested an alternative natural peroxidase source obtained from the wastes of a local highly-consumed vegetable and studied their enzymatic activity towards the preparation of biologically active, valuable compounds.

Reduction of bovine strongilides in naturally contaminated pastures in the southeast region of Brazil.

Biological control through the use of nematophagous fungi is a sustainable alternative for combatting helminthes in domestic animals and allows a reduction in the use of anthelmintics. The objective of this research was to evaluate the efficacy of the Arthrobotrys cladodes var macroides fungus in a pelleted formulation, based on sodium alginate and administered twice a week orally, as an alternative for the biological control of nematodes in field-grown young cattle. The experiment was conducted in a farm located in the municipality of Viçosa, MG, where 12 cattle, seven to nine months old, were allocated in two groups (treated group and control group) and distributed in pickets of Brachiaria decumbens, naturally infested with nematode larvae. The animals in the treated group received 1g of sodium alginate matrix pellets for every 10 kg of animal live weight, containing the nematophagous fungus Arthrobotrys cladodes var macroides and administered twice a week in conjunction with commercial feed. In the control group, each animal received 1 g of pellets for every 10 kg of animal live weight, without fungal mycelium added to the feed. Samples of feces and pastures were collected fortnightly for 12 months. The results showed that the most prevalent nematode genera in the coprocultures were Haemonchus sp., Cooperia sp. and Oesophagostomum sp., reflecting the results found in forage. The pasture that contained the animals that received feed with the fungus presented a reduction of 59% and 52% of larvae recovered at distances of 20 cm and 40 cm from the fecal pats, respectively. The mean number of eggs per gram of feces each month and animal body weight did not differ (p > 0.05) between the treated and control groups. Stool and soil samples from both groups were colonized by A. cladodes fungus and other fungi. Administration of Arthrobotrys cladodes var macroides mycelium by means of a sodium alginate matrix twice weekly reduced larval infestation of the surrounding pasture, indicating that this fungus may be a promising biological control of infecting forms of nematodes present in the environment.

Can cactus (Opuntia stricta [Haw.] Haw) cladodes plus urea replace wheat bran in steers' diet?

OBJECTIVE: The study aimed to evaluate the effect of replacing wheat bran for cactus cladodes plus urea (0%, 25%, 50%, 75%, and 100%) on the intake of nutrients, nitrogen balance, microbial protein synthesis, and rumen fermentation for steers. METHODS: Five crossbred steers (1/2 Holstein-Zebu), with rumen cannula and an average body weight of 180±5.3 kg, were assigned to a 5×5 Latin square design. Dietary treatments consisted of the replacement of the total of wheat bran in basal diet by cactus cladodes using the following proportions: 0% for basal diet, 25%, 50%, 75%, and 100% cactus cladodes replacing wheat bran. Urea was added to the diets to adjust the crude protein (CP) content to 130 g/kg dry matter. RESULTS: Maximum dry matter intake (5.73 kg/d) and maximum nitrogen balance (103 g/d) were estimated for 54.6% and 70.8% replacement levels of wheat bran. The maximum microbial protein production (44.6 g/d) was obtained at a replacement level of 49.7%, and a medium value (125 g CP mic/kg total digestible nutrients) of microbial protein efficiency was observed. The rumen pH increased linearly according to cactus cladodes inclusion, while the ammonia nitrogen medium value was 24.5 mg/dL. CONCLUSION: The replacement of 55% wheat bran for cactus cladodes plus urea in the diet of crossbred steers is recommended.

Shading of stock plants and the use of auxin in red pitaya cuttings / Sombreamento de plantas matrizes e uso de auxina na estaquia de pitaia vermelha

Although there has been a significant expansion of red pitaya cultivation in Brazil and other countries around the world, its cultivation requires scientific expertise to identify the plant production systems best suited to Brazilian soils and climate. Therefore, this study aimed to evaluate the effect of exogenous auxin and shading of the parental stock plants on the subsequent quality of red pitaya cuttings. The use of a commercial rooting auxin (with and without) and five shading levels on stock plants (full sun, 35, 50, 65 and 80% shading) were tested via four randomized blocks in a factorial 2x5 scheme with two plants per plot. After 90 days, the following vegetative characteristics were evaluated: length of the longest root (LR), root dry mass (RDM), root width (RW), shoot fresh weight (SFW) and shoot dry weight (SDW), total dry weight (TDW), number of axillary shoots (NAS), sum of the length of the axillary shoots (SLAS), and shoot:root ratio (SRR). We concluded that for the production of high quality plants, collecting cuttings from stock plants grown in full sun or 80% shade is recommended, eliminating the need to use commercial rooting auxin.(AU)

Embora tenha ocorrido grande expansão no cultivo de pitaia vermelha tanto no Brasil como em outros países do mundo, a cultura necessita de informações científicas que subsidiem a definição de sistemas de produção de mudas mais adequados às condições edafoclimáticas brasileiras. Diante disso, objetivou-se avaliar o efeito de auxina exógena e sombreamento das plantas matrizes sobre a qualidade de mudas de pitaia vermelha. Foram testados o uso de enraizador comercial (presença e ausência) e cinco níveis de sombreamento nas plantas matrizes (pleno sol, 35, 50, 65 e 80% de sombreamento), os quais foram arranjados em fatorial 2x5, com quatro blocos casualizados e duas plantas por parcela. Aos 90 dias, avaliaram-se as seguintes características vegetativas: comprimento da maior raiz (CR), massa seca das raízes (MSR), largura radicular (LR), massa fresca (MFPA) e seca da parte aérea (MSPA), massa seca total (MST), número de emissões laterais (NEL), somatório do comprimento de emissões laterais (SCEL) e razão parte aérea-raiz (RPAR). Para a produção de mudas de boa qualidade recomenda-se a coleta de estacas em plantas matrizes cultivadas a pleno sol ou 80% de sombra, sem a necessidade de enraizador comercial.(AU)

Shading of stock plants and the use of auxin in red pitaya cuttings / Sombreamento de plantas matrizes e uso de auxina na estaquia de pitaia vermelha

Although there has been a significant expansion of red pitaya cultivation in Brazil and other countries around the world, its cultivation requires scientific expertise to identify the plant production systems best suited to Brazilian soils and climate. Therefore, this study aimed to evaluate the effect of exogenous auxin and shading of the parental stock plants on the subsequent quality of red pitaya cuttings. The use of a commercial rooting auxin (with and without) and five shading levels on stock plants (full sun, 35, 50, 65 and 80% shading) were tested via four randomized blocks in a factorial 2x5 scheme with two plants per plot. After 90 days, the following vegetative characteristics were evaluated: length of the longest root (LR), root dry mass (RDM), root width (RW), shoot fresh weight (SFW) and shoot dry weight (SDW), total dry weight (TDW), number of axillary shoots (NAS), sum of the length of the axillary shoots (SLAS), and shoot:root ratio (SRR). We concluded that for the production of high quality plants, collecting cuttings from stock plants grown in full sun or 80% shade is recommended, eliminating the need to use commercial rooting auxin.

Embora tenha ocorrido grande expansão no cultivo de pitaia vermelha tanto no Brasil como em outros países do mundo, a cultura necessita de informações científicas que subsidiem a definição de sistemas de produção de mudas mais adequados às condições edafoclimáticas brasileiras. Diante disso, objetivou-se avaliar o efeito de auxina exógena e sombreamento das plantas matrizes sobre a qualidade de mudas de pitaia vermelha. Foram testados o uso de enraizador comercial (presença e ausência) e cinco níveis de sombreamento nas plantas matrizes (pleno sol, 35, 50, 65 e 80% de sombreamento), os quais foram arranjados em fatorial 2x5, com quatro blocos casualizados e duas plantas por parcela. Aos 90 dias, avaliaram-se as seguintes características vegetativas: comprimento da maior raiz (CR), massa seca das raízes (MSR), largura radicular (LR), massa fresca (MFPA) e seca da parte aérea (MSPA), massa seca total (MST), número de emissões laterais (NEL), somatório do comprimento de emissões laterais (SCEL) e razão parte aérea-raiz (RPAR). Para a produção de mudas de boa qualidade recomenda-se a coleta de estacas em plantas matrizes cultivadas a pleno sol ou 80% de sombra, sem a necessidade de enraizador comercial.

Produtividade da palma forrageira cultivada em diferentes densidades de plantio / Cactus pear forage production under different plant densities

Entre as práticas e técnicas empregadas para obtenção de maior produtividade de massa seca, a escolha do arranjamento de plantas é, entre as decisões de manejo das plantas cultivadas, uma das mais importantes, associada à escolha da cultivar e do sistema de cultivo. Em função do exposto, objetivou-se com este trabalho avaliar a produtividade de forragem de palma forrageira cultivada em diferentes densidades de plantio. O experimento foi instalado no Campo Experimental do CPATC - Embrapa, em Frei Paulo - SE. O delineamento experimental utilizado foi em blocos casualizados, com parcelas subdivididas e quatro repetições. As parcelas foram representadas por genótipos de palma forrageira (Miúda, Redonda e Gigante) e as subparcelas pelas seguintes densidades de plantio (10.000, 20.000, 40.000 e 80.000 plantas ha-1). Os genótipos de palma forrageira apresentam comportamento diferente quanto ao número de cladódios na 1a inserção e no número de cladódios por planta. O aumento da densidade de plantio reduz o comprimento e a largura do cladódio. Existe comportamento diferencial dos genótipos quanto à capacidade produtiva em diferentes densidades. A palma forrageira Miúda apresenta melhor potencial produtivo no cultivo adensado. Já a palma Redonda e Gigante tem maior teor de umidade na massa verde da forragem, e, em cultivo adensado, a palma forrageira reduz a massa verde e seca por planta.(AU)

Plant arrangement is one of the most important among management decisions of cultivated plants among the practices and techniques employed to obtain higher productivity of dry matter, due to its association to cultivar choice and cultivation system. The current research evaluates forage yield at different planting densities. The experiment was conducted in the CPATC-Embrapa experimental field in the municipality of Frei Paulo SE Brazil. Experimental design consisted of randomized blocks with split plot and four replications. The plots were the genotypes of forage cactus (small, rounded and giant size) and subplots followed planting densities (10,000; 20,000; 40,000 and 80,000 plants ha-1). Cactus forage genotypes exhibit different behavior with regard to number of cladodes in the first insertion and in the number of cladodes per plant. Increased planting density reduces the cladodes length and width. A differential behavior of genotypes exists with regard to the yield capacity for different densities. The small forager cactus has the best production potential in dense growth, whereas the round and giant-sized cactus have a higher rate of humidity in green mass. Forager cactus reduces green and dry mass per plant in dense culture.(AU)

Produtividade da palma forrageira cultivada em diferentes densidades de plantio / Cactus pear forage production under different plant densities

Entre as práticas e técnicas empregadas para obtenção de maior produtividade de massa seca, a escolha do arranjamento de plantas é, entre as decisões de manejo das plantas cultivadas, uma das mais importantes, associada à escolha da cultivar e do sistema de cultivo. Em função do exposto, objetivou-se com este trabalho avaliar a produtividade de forragem de palma forrageira cultivada em diferentes densidades de plantio. O experimento foi instalado no Campo Experimental do CPATC - Embrapa, em Frei Paulo - SE. O delineamento experimental utilizado foi em blocos casualizados, com parcelas subdivididas e quatro repetições. As parcelas foram representadas por genótipos de palma forrageira (Miúda, Redonda e Gigante) e as subparcelas pelas seguintes densidades de plantio (10.000, 20.000, 40.000 e 80.000 plantas ha-1). Os genótipos de palma forrageira apresentam comportamento diferente quanto ao número de cladódios na 1a inserção e no número de cladódios por planta. O aumento da densidade de plantio reduz o comprimento e a largura do cladódio. Existe comportamento diferencial dos genótipos quanto à capacidade produtiva em diferentes densidades. A palma forrageira Miúda apresenta melhor potencial produtivo no cultivo adensado. Já a palma Redonda e Gigante tem maior teor de umidade na massa verde da forragem, e, em cultivo adensado, a palma forrageira reduz a massa verde e seca por planta.

Plant arrangement is one of the most important among management decisions of cultivated plants among the practices and techniques employed to obtain higher productivity of dry matter, due to its association to cultivar choice and cultivation system. The current research evaluates forage yield at different planting densities. The experiment was conducted in the CPATC-Embrapa experimental field in the municipality of Frei Paulo SE Brazil. Experimental design consisted of randomized blocks with split plot and four replications. The plots were the genotypes of forage cactus (small, rounded and giant size) and subplots followed planting densities (10,000; 20,000; 40,000 and 80,000 plants ha-1). Cactus forage genotypes exhibit different behavior with regard to number of cladodes in the first insertion and in the number of cladodes per plant. Increased planting density reduces the cladodes length and width. A differential behavior of genotypes exists with regard to the yield capacity for different densities. The small forager cactus has the best production potential in dense growth, whereas the round and giant-sized cactus have a higher rate of humidity in green mass. Forager cactus reduces green and dry mass per plant in dense culture.

First report of interaction of nematophagous fungi on Libyostrongylus douglassii (Nematoda: Trichostrongylidae) / Primeiro relato da interação de fungos nematófagos sobre Libyostrongylus douglassii (Nematoda: Trichostrongilidae)

Libyostrongylus douglassii is a gastrointestinal nematode parasite of ostriches that can cause up to 50% mortality in young birds. The objective of this study was to compare the predatory capacity of two isolates of the predatory fungi Duddingtonia flagrans(AC001 and CG722 isolates) and one of Arthrobotrys cladodes (CG719) on infective larvae (L3) of L. douglassii under laboratory conditions, in 2% water-agar medium. The results showed that the fungi tested were effective in preying upon the L3 of L. douglassii (P < 0.05), compared with the control group. However, there was no difference in predatory capacity between the fungi tested (P > 0.05) during the seven days of experimental testing. In comparison with the control, without fungus, there were significant decreases (P < 0.05) of 85.2% (AC001), 81.2% (CG722) and 89.2% (CG719) in the average numbers of L3 of L. douglassii recovered from treatments with the isolates tested. In the present study, the three isolates of the predatory fungi D. flagrans (AC001 and CG722) and A. cladodes (CG719) were efficient at in vitro destruction of the L3 of L. douglassii.

Libyostrongylus douglassii é um nematóide parasito gastrintestinal de avestruzes que pode causar até 50% de mortalidade em aves jovens. O objetivo deste trabalho foi comparar a capacidade predatória de dois isolados de fungos predadores Duddingtonia flagrans (isolados AC001 e CG722) e um Arthrobotrys cladodes (CG719) sobre larvas infectantes (L3) de L. douglassii em condições laboratoriais, em meio ágar­água 2%. Os resultados demonstraram que os fungos testados foram eficientes em predar as L3 de L. douglassii (P < 0,05) em relação ao grupo controle. Contudo, não foi observada nenhuma diferença na capacidade predatória entre os fungos testados (P > 0,05) durante os sete dias do ensaio experimental. Em comparação ao controle, sem fungo, houve uma redução significativa (P < 0,05) de 85,2% (AC001); 81,2% (CG722) e 89,2% (C719) na média de L3 recuperadas nas placas do grupo tratado com os isolados testados. No presente trabalho, os três isolados de fungos predadores D. flagrans (AC001 e CG722) e A. cladodes (CG719) foram eficientes na destruição in vitro das L3 de L. douglassii.

First report of interaction of nematophagous fungi onLibyostrongylus douglassii (Nematoda: Trichostrongylidae)

Libyostrongylus douglassii is a gastrointestinal nematode parasite of ostriches that can cause up to 50% mortality in young birds. The objective of this study was to compare the predatory capacity of two isolates of the predatory fungi Duddingtonia flagrans(AC001 and CG722 isolates) and one of Arthrobotrys cladodes (CG719) on infective larvae (L3) of L. douglassii under laboratory conditions, in 2% water-agar medium. The results showed that the fungi tested were effective in preying upon the L3 of L. douglassii (P 0.05), compared with the control group. However, there was no difference in predatory capacity between the fungi tested (P > 0.05) during the seven days of experimental testing. In comparison with the control, without fungus, there were significant decreases (P 0.05) of 85.2% (AC001), 81.2% (CG722) and 89.2% (CG719) in the average numbers of L3 of L. douglassii recovered from treatments with the isolates tested. In the present study, the three isolates of the predatory fungi D. flagrans (AC001 and CG722) andA. cladodes (CG719) were efficient at in vitro destruction of the L3 of L. douglassii.

Libyostrongylus douglassii é um nematóide parasito gastrintestinal de avestruzes que pode causar até 50% de mortalidade em aves jovens. O objetivo deste trabalho foi comparar a capacidade predatória de dois isolados de fungos predadores Duddingtonia flagrans (isolados AC001 e CG722) e umArthrobotrys cladodes (CG719) sobre larvas infectantes (L3) de L. douglassii em condições laboratoriais, em meio ágarágua 2%. Os resultados demonstraram que os fungos testados foram eficientes em predar as L3 de L. douglassii (P 0,05) em relação ao grupo controle. Contudo, não foi observada nenhuma diferença na capacidade predatória entre os fungos testados (P > 0,05) durante os sete dias do ensaio experimental. Em comparação ao controle, sem fungo, houve uma redução significativa (P 0,05) de 85,2% (AC001); 81,2% (CG722) e 89,2% (C719) na média de L3 recuperadas nas placas do grupo tratado com os isolados testados. No presente trabalho, os três isolados de fungos predadores D. flagrans (AC001 e CG722) e A. cladodes (CG719) foram eficientes na destruição in vitro das L3 deL. douglassii.