Effect of low-glucosinolate crambe meal in diet on lactational performance, efficiency of nutrient utilization, and hepatic function of crossbred Holstein × Zebu and Jersey cows.

Crambe meal (CM) is a potential dietary protein source for ruminant, but its effects in diets for lactating dairy cows remains unknown. We evaluated the effects of inclusion of the low-glucosinolates (GIs) CM (450 mg GIs/kg DM) in partial total mixed ration (pTMR) on performance, efficiency of nutrient utilization, and hepatic function of crossbred Holstein × Zebu and Jersey cows. Eight crossbred Holstein × Zebu cows and four Jersey cows were blocked by breed and days in milk, and randomly assigned in a replicated 4 × 4 Latin square design, and distributed in one of four isonitrogenous TMRs (130 g CP/kg DM): 0, 45, 90, and 135 g CM/kg DM pTMR. Crambe meal was included in pTMR replacing soybean meal (SBM) and ground corn grain (GCG). The pTMRs were offered ad libitum between 7:00 a.m. and 5:00 p.m. Cows were kept on pasture of Panicum maximum cv. Mombaça (90.4 g CP/kg DM) between 6:00 p.m. and 6:30 a.m. Pasture intake was measured from external and internal fecal excretion marker. Inclusion of CM did not affect (P ≤ 0.05) the DM intakes of pTMR and pasture, apparent total-tract digestibilities of organic matter, CP and NDF, ruminal outflow microbial protein, milk yield, milk composition, urinary N excretion, milk N efficiency, and level of plasm hepatic enzymes. The effects of CM inclusion in pTMR were independent of breed. Low-glucosinolates CM can be incorporated up to 135 g/kg DM in pTMR in replacement of SBM and GCG without affecting performance, efficiency of nutrient utilization, and hepatic function of crossbred Holstein × Zebu and Jersey cows.

Nutritional evaluation of crambe meal as a partial replacement of soybean meal in Nile tilapia diet.

A variety of plant protein sources have been evaluated in aquafeeds. Crambe meal (CM) has potential for inclusion in fish diets because of its nutritional composition. This study evaluated the apparent digestibility coefficient (ADC) of crambe meal and its potential to partially replace soybean meal (SM) protein in Nile tilapia Oreochromis niloticus diets. The ADC for dry matter, crude protein, ether extract, energy, amino acids, calcium and phosphorus of CM were assessed in fish (n = 80; 65.30 ± 5.32 g). Subsequently, an 80-day feeding trial was conducted with Nile tilapia (n = 140; 6.04 ± 0.25 g) randomly distributed in 20 experimental cages (70 L; seven fish cage-1) allocated in five circular tanks (1000 L) in a recirculation water system, to evaluate the effects of replacement of SM by CM (0, 6, 12, 18 and 24% in isonitrogenous and isoenergetic diets) on growth, blood parameters, fillet yield and proximal composition. The CM shows good digestibility of protein (0.824) and amino acids (0.844) by Nile tilapia and its inclusion in the diet does not affect carcass and fillet yield or proximal composition. Fish fed diets with 24.0% of the SM replaced by CM showed the worst weight gain and feed conversion rate. The protein efficiency ratio decreased in fish fed diets with 12.0, 18.0 and 24.0% of the SM replaced by CM. Hemoglobin, mean corpuscular hemoglobin concentration, total plasma protein, glucose and alanine aminotransferase enzyme activity trend to increase at highest levels of CM in the diet. In conclusion, CM has high digestibility of protein and amino acids for Nile tilapia. However, anti-nutritional factors present in untreated CM interfere on the growth and nutrient utilization of Nile tilapia.

Production components of crambe in paraiba semi-arid region with nitrogen fertilization and different spacings / Componentes de produção de crambe no semiárido paraibano com adubação nitrogenada e diferentes espaçamentos

Crambe (Crambe abyssinica H.), a promising species in the production of biodiesel, has low planting cost, high oil content and easy extraction, besides being an alternative for the crop rotation system. The objective of this study was to evaluate the effects of nitrogen fertilization and line spacing on the production components and yield of the crambe crop under semi-arid conditions. The study was conducted under field conditions in the experimental area of the National Semi-Arid Institute-INSA, located in the city of Campina Grande-PB. The experimental design was a randomized complete block design in 4 x 2 factorial scheme, consisting of four nitrogen doses (30, 60, 90, 120 kg ha-1) with two spacings between planting lines (0.20 and 0.40 meters) with three replications, totaling 24 experimental plots. The evaluated parameters were: number of siliques per plant, dry weight of shoots, number of branches (primary and secondary), primary stem length, number of siliques per stem, mass of 1000 grains and productivity. The data were submitted to analysis of variance, in which the nitrogen doses (quantitative factor) were compared by regression analysis. The different spacings were compared by means of a t-test at the 0.05 probability level by using statistical software SISVAR version 5.2. Nitrogen fertilization at 0.40 m spacing between the planting lines led to the best results for the production components, and the highest productivity was obtained at 0.20 m spacing of with nitrogen fertilization of 120 kg ha-1.

O crambe (Crambeabyssinica H.), espécie promissora na produção de biodiesel, apresenta baixo custo de plantio, elevado teor de óleo e de fácil extração, além de ser alternativa para o sistema de rotação de culturas. Mediante a necessidade de conhecimento para ampliação do cultivo dessa espécie, este estudo teve como objetivo avaliar os efeitos da adubação nitrogenada e espaçamento entre linhas no cultivo nos componentes de produção e na produtividade da cultura do crambe em condições semiáridas. O estudo foi conduzido em condições de campo na área experimental pertencente ao Instituto Nacional do Semiárido-INSA, situado no município de Campina Grande-PB. O delineamento experimental utilizado foi em blocos casualizados, no esquema fatorial 4 x 2, sendo constituído de quatro doses de nitrogênio (30; 60; 90; 120 kg ha-1) com dois espaçamentos entre linhas de plantio (0,20 e 0,40 metros) e com três repetições, totalizando 24 parcelas experimentais. Os parâmetros avaliados foram: número de síliquas por planta, massa seca da parte aérea, número de ramificações (primárias e secundárias), comprimento de haste primária, número de silíquas por haste, massa de 1000 grãos e produtividade. Os dados obtidos foram submetidos à análise de variância, em que as doses de nitrogênio (fator quantitativo) foram comparadas por análise de regressão. Os diferentes espaçamentos foram comparados por meio de comparação de médias, pelo teste t, ao nível de 0,05 de probabilidade utilizando-se software estatístico SISVAR versão 5.2. A adubação nitrogenada sob espaçamento de 0,40 m, entre as linhas de plantio, proporcionou os melhores resultados quanto aos componentes de produção, e a maior produtividade foi obtida no espaçamento referente a 0,20 m com a adubação nitrogenada de 120 kg ha-1.

Phytoremediation capacity, growth and physiological responses of Crambe abyssinica Hochst on soil contaminated with Cd and Pb.

The search for vegetal species regarding effectiveness in the phytoremediation of soils is of great importance, mainly in function of the great environmental problems, such as soil contamination with heavy metals, the necessity of producing more food, among others that mankind face today. This work aimed (i) to evaluate phytoremediation capacity of Crambe abyssinica Hochst and its growth in soil artificially contaminated with Cd and Pb, and (ii) to evaluate the possible impacts of crambe cultivation in contaminated soil conditions, in order to evaluate, to test, and to question the Brazilian CONAMA 420, providing important information that can be useful for governmental and environmental purposes. Two simultaneous experiments were developed, one for each metal. The soils were contaminated with salts of CdCl2 and PbCl2H2O in five doses based on the investigation values (IV) of CONAMA Resolution 420, resulting in 0; 1.5; 3; 9 and 30 mg kg-1 for Cd and 33; 90; 180; 540 and 1800 mg kg-1 for Pb. Gaseous exchange, development, nutritional composition and production of plant components, as well as phytoavailability of metals, were evaluated. The contamination with metals reduced photosynthesis, increased breathing as well as leading to a negative effect on the mineral nutrition and productivity in general; Plants cultivated in soil with Cd presented higher phytoavailability when compared to those cultivated in the Pb conditions, being found metals in all parts of the crambe plants from 1.5 mg kg- 1 of Cd in the soil; and Pb was retained only in roots, not being translocated in the plant. Cd showed higher phytoavailability, being found in all parts of the plant and Pb was retained only in the roots. Cd showed a higher phytoavailability when compared to Pb, also being found in all parts of crambe plants from dose 1.5 mg kg-1 of Cd in soil, which is an environmental problem, since in these concentrations the cultivation of crops is allowed by Brazilian legislation CONAMA 420.

Chemical change during storage of crambe cultivated with application of plant growth regulators / Alterações químicas durante o armazenamento de crambe cultivado com aplicação de reguladores vegetais

This study aimed to characterize the chemical composition of crambe grains produced with plant growth regulators in the 2014 and 2015 harvests and stored for 180 days. During crambe cultivation, two foliar sprayings were carried out with the following treatments: 1) control treatment with distilled water; 2) indole-3-acetic acid 100 mg L-1 (IAA); 3) 3-gibberellic acid P.A. 100 mg L-1 (GA3); 4) Stimulate®. After harvesting, the grains were analyzed initially and after 180 days of uncontrolled storage. We checked water content, lipids in hot extraction (HE), lipids in cold extraction (CE), proteins, acidity and antioxidant activity. The Tukey test was used to compare the averages (p<0.05) and the parameters were grouped using a multivariate clustering technique presented in dendogram. The CE and HE lipid contents were reduced after storage, whereas the protein content and antioxidant activity of the grains were not altered by storage. However, the grains that received IAA application showed an increase in the CE lipid content and their preservation after storage. GA3 application, on the other hand, reduced the protein content. Lipid contents remained constant at the end of storage, and the acidity was reduced due to the application of GA3 and Stimulate® plant growth regulators. The 180-day storage changed crambe quality and regulators can assist in preserving grain quality during storage. The CE and HE lipid content and the grain acidity were good indicators for evaluating effects.

O objetivo do presente trabalho foi caracterizar a composição química dos grãos de crambe produzidos com reguladores vegetais nas safras de 2014 e 2015 e armazenados por 180 dias. Durante o cultivo do crambe, realizaram-se duas pulverizações foliares seguindo os tratamentos: 1) tratamento controle com água destilada; 2) ácido indol-3-acético 100 mg L-1 (AIA); 3) ácido 3-giberélico P.A. 100 mg L-1 (GA3); 4) Stimulate®. Após colhidos, os grãos foram analisados inicialmente e após 180 dias de armazenamento não controlado. Aferiu-se o teor de água, lipídeos em extração a quente (EQ), lipídeos em extração a frio (EF), proteínas, acidez e atividade antioxidante. As médias foram comparadas pelo teste Tukey (p<0,05) e os parâmetros agrupados pela técnica multivariada de agrupamento apresentado em dendograma. Os teores de lipídeos EF e EQ reduziram com o armazenamento enquanto que o teor de proteína e a atividade antioxidante dos grãos não foram alterados por esse fator. A aplicação de IAA destacou-se pelo aumento no teor de lipídeos EF e conservação desse após o armazenamento. A aplicação de GA3 reduziu o teor de proteínas. Os teores de lipídeos se mantiveram constante no final do armazenamento e houve redução no teor de acidez em decorrência da aplicação dos reguladores vegetais GA3 e Stimulate®. O armazenamento de 180 dias altera a qualidade de crambe e os reguladores podem auxiliar na preservação da qualidade dos grãos durante o armazenamento. O teor de lipídeos EF, EQ e a acidez dos grãos mostraram-se bons indicadores para avaliação dos efeitos.

Development of Industrial Oil Crop Crambe abyssinica for Wax Ester Production through Metabolic Engineering and Cross Breeding.

As an important industrial feedstock, wax esters (WEs) have been used as lubricants in a number of technical processes. There is however currently no large-scale biological source for WE production and alteration in metabolic pathways of plant oils for producing WEs could be attractive to the commercial markets. Here, we present the breeding results of long-term studies on successful development of new crambe lines producing WEs through genetic engineering and cross breeding. The transgenic crambe lines producing WEs at over 25% of the total seed oil were first generated by introduction of the jojoba WE biosynthetic genes ScFAR and ScWS. Further improvement of the lines aiming at improving oxidative stability of WEs was achieved through introducing the CaFAD2-RNAi gene into these lines by crossing. The hybrid lines possessed similar agronomic traits to the wild type and a stable level of WEs over several generations, suggesting a high potential of crambe as an industrial crop for WE production.

Foliar application of plant growth regulators changes the physiological quality of crambe seeds

The application of plant growth regulators can manipulate the development of cultivated plants, and their physiological effects may be reflected in the physiological quality of the seeds. This study evaluated the effects of plant growth regulators (PGR) on the physiological quality of crambe seeds produced in the 2014 and 2015 harvests. During crop development, we applied two foliar sprayings of the following treatments: 1) control treatment with distilled water; 2) indole-3-acetic acid 100 mg L-1 (IAA); 3) 3-gibberellic acid P.A. 100 mg L-1 (GA3); 4) commercial PGR Stimulate® 6 mL L-1. The seeds were harvested and evaluated for water content, germination percentage and germination speed index (GSI), electrical conductivity and concentrations of leached Ca, Mg and K ions. Means were compared by Tukey ́s and Dunnett tests (p<0.05). Applying Stimulate® increased the germination percentage and the germination speed index, while IAA application showed an opposite effect, increasing the electrical conductivity of crambe seeds. Calcium leaching decreased with IAA application, and all regulators reduced K leaching. We therefore infer that the application of plant growth regulators influences the physiological quality of crambe seeds.

Spectroscopic Methods in the Evaluation of Modified Vegetable Base Oils from Crambe abyssinica.

Raw vegetable oil from Crambe abyssinica was subjected to oxidative treatment to enhance its viscosity. The oxidation processes were carried out in the presence of N-hydroxyphthalimide with or without supercritical CO2 as a solvent. Four spectroscopic techniques (Raman, UV-VIS, FT-IR, NMR) were applied to assess the chemical changes taking place during the oxidation. Raman and NMR spectroscopy proved best in the assessment of the chemical transformations leading to increased viscosity of the modified vegetable oil.

Designing Lubricating Properties of Vegetable Base Oils.

Lubricants that are based on renewable raw materials have drawn increased attention in various applications, especially those related to the food industry. Due to the high requirements of environmental protection, there is a need to develop biodegradable base oils that are environmentally friendly and do not contain harmful components. The objective of the research was to obtain a base oil with a certain viscosity and certain desired lubricating properties. Base oils were obtained from Crambe abyssinica oil by means of blending with synthetic oil and oxidation. The oxidation processes were carried out in the presence of N-hydroxyphthalimide with or without CO2 as a solvent. As a final result of this study, oil bases meeting the viscosity requirements and showing suitable lubricating properties were obtained. The Raman spectra of the obtained oils were evaluated.

Sorghum silage supplemented with crambe meal improves dry matter intake and milk production in crossbred Holstein cows.

We studied the feed intake and milk production of Holstein × Zebu crossbred cows fed sorghum silage diets and concentrates, with and without the addition of crambe meal. Using a change-over design for a total of 120 days, eight cows were fed two diets (concentrates + silages with and without crambe meal) in four 30-day periods, with four replications (animals). The crambe meal diet increased (P < 0.05) the dry matter intake (DMI), crude protein intake (CPI), and non-fibrous carbohydrates intake (NFCI) as well as milk production (4.3%). No difference was noted (P > 0.05) for the milk composition between the treatments. Crambe meal as an additive in sorghum silage (100 g/kg in natural matter) showed a great potential for introduction in dairy farming as it substantially increases CP intake, does not reduce food intake, raises animal productivity, and does not affect milk composition.

Natural infection of croton yellow vein mosaic virus and its cognate betasatellite in germplasm of different Crambe spp in India.

Crambe is an important crop grown worldwide for industrial oil and seed meal. Besides the fungal and bacterial diseases, the crop is reported to be infected by tobacco mosaic virus, beet western yellows virus and turnip mosaic virus under experimental condition. Till now, there was no report of natural infection of any begomovirus in this crop. In the present study, a leaf curl disease was observed in germplasm accessions of three species of Crambe (C. abyssinica, C. glabrata and C. hispanica). Based on the symptoms and presence of whitefly population in the field, begomovirus infection was suspected. Molecular characterization through RCA approach, indicated presence of croton yellow vein mosaic virus (CYVMV, KJ747958) and croton yellow vein mosaic betasatellite (CroYVMB, KM229762). Co-agroinoculation of partial dimeric construct of CYVMV with complete dimeric construct of CroYVMB, produced typical leaf curl symptoms in C. abyssinica, whereas, agroinoculation of partial dimeric construct of CYVMV alone could not produce symptoms in the same plant. In contrast, the CYVMV construct alone could produce symptom in Nicotiana benthamiana, a model host for plant virus studies. In N. benthamiana co-inoculation of CroYVMV with CYVMV construct develop more severe symptoms. However, neither the CYVMV construct alone nor the co-inoculation with CroYVMB produce any symptom in Arabidopsis thaliana even with different methods of inoculation. Inoculated Arabidopsis thaliana also did not yield any amplification of the virus as assessed through PCR and rolling circle amplification (RCA). Thus it confirmed that for successful infection in crambe, CYVMV requires betasatellite, while in N. benthamiana, it does not require betasatellite for symptom induction and in Arabidopsis thaliana CYVMV alone or in presence of betasatellite did not replicate and produce any symptom. This study constitutes the first confirmed record of natural infection of a begomovirus in crambe and further confirmed that cognate betasatellite of CYVMV has differential role in infectivity in different hosts.

Synthesis of structured lipids containing behenic acid from fully hydrogenated Crambe abyssinica oil by enzymatic interesterification.

Low-calorie structured lipids (SLs) rich in behenic and oleic acids were produced by enzymatic (EI) and chemical interesterification (CI) of high oleic sunflower oil and fully hydrogenated Crambe abyssinica oil in proportions of 60:40, 50:50, 40:60 and 30:70 (w/w), respectively. The immobilized lipase from Thermomyces lanuginosus (Lipozyme TL IM) was used for 3 h at 70 °C at a dosage of 7% (w/w) free of organic solvents. The original blend (BE) and the reaction products were evaluated for their fatty acid (FA) and triacylglycerols (TAG) compositions, solid fat contents, thermal analysis, regiospecific distribution of the FA, microstructure and polymorphism. The tendency was towards a reduction in the C52, C54, C60, C62 and C64 TAG contents and an increase in the C56, C58 e C66 TAG contents, therefore the solid fat content was reduced. There was lower acyl migration at the sn-2 position of the TAGs in EI as compared to CI in all the blends ratios. Needle-like crystals were predominant in the IE while large symmetrical spherulites were observed in the BE. Reduction in the mean crystal diameter was observed in all the blends, in addition to modifications of the crystal morphology. X-ray diffraction analysis showed a predominance of the ß' form crystals in both the IE and CI. The SLs were produced for application in the food industry as bakery/confectionery fats in the proportions of 60:40/50:50 and as additive in the crystallization of lipids in the proportions of 40:60/30:70.

Metabolic engineering of fatty alcohol production in transgenic hairy roots of Crambe abyssinica.

Biotechnological production of fatty alcohols, important raw materials in the chemical industry, has been receiving considerable attention in recent years. Fatty alcohols are formed by the reduction of fatty acyl-CoAs or fatty acyl-ACPs catalyzed by a fatty acyl reductase (FAR). In this study, we introduced genes encoding FARs from Arabidopsis thaliana (AtFAR5) and Simmondsia chinensis (ScFAR) into Crambe abyssinica hairy roots via Agrobacterium rhizogenes-mediated transformation. The efficiency of the transformation ranged between 30 and 45%. The fatty alcohols were only detected in the transgenic hairy root lines expressing ScFAR gene. In all tested lines stearyl alcohol (18:0-OH), arachidyl alcohol (20:0-OH), and behenyl alcohol (22:0-OH) were produced. The content of 18:0-OH varied from 1 to 3% of total fatty acids and fatty alcohols, while the amount of either 20:0-OH and 22:0-OH did not exceed 2%. The transgenic hairy root lines produced from 0.98 to 2.59 nmol of fatty alcohols per mg of dry weight. Very low activity of ScFAR was detected in the microsomal fractions isolated from the selected hairy root lines. To our knowledge, this is the first report on the fatty alcohol production in the hairy root cultures. Biotechnol. Bioeng. 2017;114: 1275-1282. © 2016 Wiley Periodicals, Inc.

High-throughput development of simple sequence repeat markers for genetic diversity research in Crambe abyssinica.

BACKGROUND: The allohexaploid Crambe abyssinica (crambe) is an oilseed crop that has been recognized for its potential value in the chemical industry, particularly in terms of producing high-erucic acid content vegetable oil. However, as an understudied crop, improvement of crambe has been hampered by the lack of genetic and genomic information to enhance its yield, oil quality and resistance against biotic and abiotic stress. Development of molecular markers is therefore of great significance to facilitate genetic improvement of crambe. RESULTS: In this study, high-throughput sequencing was performed to generate sequences for the transcriptome and genome of a widely planted crambe cultivar, Galactica. A total of 186,778 expressed sequence tag (EST) contigs as 8,130,350 genomic contigs were assembled as well. Altogether, 82,523 pairs of primers were designed in the flanking sequences of the simple sequence repeat (SSR) within these contigs. Virtual PCR analysis showed that a fraction of these primers could be mapped onto the genomes of related species of Brassica, including Brassica rapa, B. oleraceae and B. napus. Genetic diversity analysis using a subset of 166 markers on 30 independent C. abyssinica accessions exhibited that 1) 95 % of the designed SSRs were polymorphic among these accessions; 2) the polymorphism information content (PIC) value of the markers ranged from 0.13 to 0.89; 3) the genetic distances (coefficient NEI72) between accessions varied from 0.06 to 0.36. Cluster analysis subsequent on the accessions demonstrated consistency with crambe breeding history. F-statistics analysis revealed a moderate level of genetic differentiation in C. abyssinica (Gst = 0.3934) and a accordingly low estimated gene flow (Nm = 0.7709). CONCLUSION: Application of high-throughput sequencing technology has facilitated SSR marker development, which was successfully employed in evaluating genetic diversity of C. abyssinica as demonstrated in our study. Results showed these molecular markers were robust and provided powerful tools for assessing genetic diversity and estimating crambe breeding history. Moreover, the SSR primers and sequence information developed in the study are freely available to the research community.

pH of exudate test in the physiological quality of crambe seeds / Teste do pH do exsudato para avaliação da qualidade fisiológica de sementes de crambe

ABSTRACT: Crambe is a rapeseed with high oil content and can be used as a winter cover or as a source of raw material for the production of biodiesel, however espite the growing interest in the culture, research on the subject is still incipient, especially concerning the seed production and analysis technology. The purpose of this study is to evaluate the physiological quality of crambe seeds, 'FMS Brilhante' cultivar, by testing the pH of exudate. Five seed lots were submitted to the determination of water content and the tests of germination and vigor (first count, emergence and tetrazolium). In the conduction of pH exudate test, temperatures (25 and 30oC), and periods of seed imbibition in water (15, 30 and 45 minutes) were tested. The experiment was conducted in a completely randomized manner, with four replicates, and the mean values were compared by the Tukey test at 5% probability; Pearson correlation between the pH of the exudate and initial tests was also made. Testing the pH of exudate is promising for separating lots of crambe seeds and the following combinations of 25°C/30 minutes or 30°C/45 minutes can be used.

RESUMO: O crambe é uma brássica com alto teor de óleo, podendo ser utilizada como cobertura de inverno ou fonte de matéria prima para a produção de biodiesel, porém, apesar do crescente interesse pela cultura, as pesquisas ainda são incipientes, principalmente quanto à tecnologia de produção e análise de sementes. O objetivo neste trabalho foi avaliar a qualidade fisiológica de sementes de crambe, cultivar 'FMS Brilhante', pelo teste do pH do exsudato. Para tanto, cinco lotes de sementes foram submetidos à determinação do teor de água e a testes de germinação e vigor (primeira contagem, emergência e tetrazólio). Na condução do teste do pH do exsudato, foram testadas as temperaturas constantes de 25 e 30ºC e períodos de embebição das sementes em água de 15, 30 e 45 minutos. O experimento foi realizado no delineamento inteiramente casualizado com quatro repetições e as médias foram comparadas pelo teste de Tukey a 5% de probabilidade; também foi feita a correlação de Pearson entre o pH do exsudato e as avaliações iniciais. O teste do pH do exsudato é promissor na separação de lotes de semente de crambe, podendo ser utilizadas as combinações 25ºC/30 minutos ou 30oC/45 minutos.

Consumo, digestibilidade e balanço de nitrogênio em ovinos alimentados com rações contendo torta de Crambe / Intake, digestibility and nitrogen balance in sheep fed with diets containing Crambe cake

O objetivo deste trabalho foi avaliar os efeitos da utilização de torta de crambe na ração de ovinos sobre o consumo e a digestibilidade. O delineamento experimental utilizado foi o quadrado latino 4x4 com quatro animais, quatro períodos e quatro tratamentos. Os tratamentos avaliados foram rações com 30% de silagem de sorgo e 70% de torta de crambe (30:70) e rações com 70% de silagem de sorgo e 30% de torta de crambe (70:30) em cordeiros sob consumo voluntário e controlado. Os animais que receberam a ração com a relação 30:70 consumiram mais matéria seca (MS), proteína bruta (PB), extrato etéreo (EE) por kg/dia, % PV/dia e g/kg PV0,75. A relação 30:70 proporcionou maior coeficiente de digestibilidade de MS e FDN. Os animais no regime voluntário apresentaram maior consumo de MS, PB, EE por kg/dia, % PV/dia e g/kg PV0,75. A torta de crambe apresentou coeficientes de digestibilidade superiores à silagem de sorgo, independentemente do regime de consumo. A relação 30:70 apresentou diferença para o N-ingerido e N-absorvido. A torta de crambe apresenta alta digestibilidade dos nutrientes e aceitação pelos animais quando associada à silagem de sorgo.(AU)

The aim of this study was to evaluate the effects of using crambe cake in diets for sheep on intake and digestibility. The experimental design was a 4x4 Latin square with four animals, four periods and four treatments. The treatments were diets with 30% sorghum silage and 70% crambe cake (30:70) and a diet with 70% sorghum silage and 30% crambe cake (70:30) in lambs under the voluntary and controlled intake. The animals who fed a diet with a 30:70 ratio consumed more dry matter (DM), crude protein (CP), ether extract (EE) per kg/day, %BW/day and g/kgPV0,75. The ration provided higher digestibility of DM and NDF. The animals in the voluntary regime presented higher consumption of DM, CP, EE per kg/day, %BW/day and g/kgPV0,75. The cake crambe presented digestibility coefficient higher than sorghum silage, regardless of the regime intake. The 30:70 ration presented a difference in N intake and N absorbed. The cake crambe has high nutrient digestibility and acceptance by animals when associated with sorghum silage.(AU)

Cover crops and straw management on yield components of crambe / Culturas de cobertura e manejo da palhada sobre os componentes de produção de crambe

The objective of this study was to evaluate the dry matter (DM) yield and nutrient accumulation in cover crops and the effect of these, as well as mechanical management of crop residues on growth and grain yield of crambe (Crambe abyssinica). The experiment was conducted in Santa Helena, Paraná, Brazil in Rhodic Hapludox (Red Latosol in the Brazilian classification) in experimental design of randomized complete blocks with four replications in a split-plot. The plots were: Pearl millet (Pennisetum glaucum), grass brachiaria (Brachiaria brizantha), forage sorghum (Sorghum bicolor) and sunn hemp (Crotalaria juncea) and an area fallow (spontaneous weed) and the sub-plots, the mechanical or not management of crop residues on the soil surface (Triton©). Grass brachiaria produced less amount of DM and showed less accumulation of nutrients. Other cover crops did not differ in DM production, but the sunn hemp showed the highest concentrations of N and Ca, while pearl millet P. The mechanical management of crop residues on the soil surface not influence the yield components of crambe, however provides higher final plant density. The crambe grown after sunn hemp showed higher DM accumulation (1,826 kg ha­1) and grain yield (1,066 kg ha­1).

Objetivou-se com este trabalho avaliar a produção de matéria seca (MS) e o acúmulo de nutrientes por culturas de cobertura e o efeito destas, bem como do manejo mecânico da palhada nos componentes da produção e produtividade de grãos de crambe. O experimento foi conduzido em Santa Helena, Paraná, Brasil, em Latossolo Vermelho distrófico típico, em delineamento experimental de blocos ao acaso com quatro repetições em parcelas subdivididas. As parcelas foram: milheto (Pennisetum glaucum (L.) R. Brown), braquiária (Brachiaria brizantha cv. Marandu), sorgo forrageiro (Sorghum bicolor (L.) Moench), crotalária (Crotalaria juncea L.) e uma área em pousio (vegetação espontânea), e as subparcelas, pela ausência e presença do manejo mecânico de palhada (Triton©). A braquiária produz menor quantidade de MS e apresenta menor acúmulo de nutrientes. As demais culturas não diferem entre si na produção de MS, contudo a crotalária apresenta os maiores acúmulos de N e Ca, enquanto que o milheto maior acúmulo de P. O manejo mecânico da palhada não influencia os componentes de produção do crambe, porém proporciona maior densidade final de plantas. O crambe cultivado em sucessão a crotalária apresenta maior acúmulo de MS (1.826 kg ha­1) e produtividade de grãos (1.066 kg ha­1).

Determinação das isotermas de equilíbrio higroscópico de frutos de crambe pelo método dinâmico / Determination of hygroscopic equilibrium isotherms of crambe fruit by dynamic method

Objetivou-se neste trabalho determinar as isotermas de sorção dos frutos de crambe pelo método dinâmico para diversas condições de temperatura e atividades de água e ajustar diferentes modelos matemáticos aos dados experimentais, bem como obter os valores do calor isostérico de dessorção em função do teor de água de equilíbrio do produto. O teor de água de equilíbrio dos frutos de crambe foi determinado pelo método dinâmico-gravimétrico para temperaturas de 25, 30, 35, 40 e 45 °C e atividades de água para cada temperatura, entre 0,270 a 0,825. A partir dos resultados obtidos calculou-se o calor isostérico para cada teor de água de equilíbrio. Aos dados experimentais foram ajustados diversos modelos matemáticos disponíveis na literatura (Chung-pfost, Copace, GAB, Halsey Modificado, Sabbah, Sigma Copace, Cavalcanti Mata, Henderson Modificado, Henderson, BET, Oswin e GAB mod). Observou-se que o teor de água de equilíbrio decresce com o aumento da temperatura para uma dada atividade de água à semelhança dos produtos higroscópicos. O modelo de Sigma Copace foi o que descreveu melhor a higroscopicidade dos frutos de crambe. Observou-se que o calor isostérico aumenta com a diminuição do teor de água do produto, indicando aumento da energia necessária para a remoção de água. Os valores de calor isostérico para os frutos de crambe na faixa de teor de água de 3,85 a 11,86 (% b.s.) variaram de 4731,73 a 2698,35kJ kg-1.

The objective of this work was to determine the sorption isotherms of crambe fruits by dynamic method for several conditions of temperature and water activity and adjust different mathematical models to experimental data, and obtain the values of isosteric heat of desorption versus the equilibrium moisture content of the product. The equilibrium moisture content of crambe fruits was determined by dynamic-gravimetric method to temperatures of 25, 30, 35, 40 and 45 °C and water activity for each temperature, from 0.270 to 0.825. From the results obtained it was calculated for each isosteric heat of moisture content equilibrium. The experimental data were adjusted several models available in the literature (Chung-pfost, Copace, GAB, Modified Halsey, Sabbah, Sigma Copace, Cavalcanti Mata, Modified Henderson, Henderson, BET, Oswin e Modified GAB). It was observed that the equilibrium moisture content decreases with increasing temperature for a given water activity like the hygroscopic products. The model Sigma Copace was best described what the hygroscopicity of the crambe fruits. It was observed that the isosteric heat increases with decreasing moisture content of the product, indicating an increase in energy required for water removal. The values of isosteric heat of crambe fruits in the range of moisture content from 3.85 to 11.86 (d.b.%) varied from 4731.73 to 2698.35 kJ kg-1.

Modelagem matemática da secagem de frutos de crambe em camada delgada / Mathematical modeling of crambe fruits in thin layer drying

Objetivou-se no presente trabalho ajustar diversos modelos matemáticos ao processo de secagem dos frutos de Crambe abyssinica, em diversas condições de ar, bem como determinar o coeficiente de difusão efetivo e obter a energia de ativação. O trabalho foi realizado no Laboratório de Pós-colheita de Produtos Vegetais do Instituto Federal de Educação, Ciência e Tecnologia Goiano ­ Câmpus Rio Verde, Goiás, Brasil (IF Goiano ­ Câmpus Rio Verde). A colheita do crambe (Crambe abyssinica Hochst) da cultivar FMS Brilhante foi realizada manualmente, com o teor de água de 38,0±1,0 (% b.s.) e secados até o teor de 8,0±1,5 (% b.s.). A secagem foi realizada sob diferentes condições controladas de temperatura 35, 45, 60, 75 e 90 °C e umidades relativas de 20,9; 8,7; 6,8; 4,8 e 2,3%, respectivamente. Dentre os modelos analisados, Page foi o melhor que se ajustou aos dados nas diferentes condições do ar de secagem. O coeficiente de difusão efetivo aumentou com a elevação da temperatura apresentando valores de 2,84 x 10-11; 3,22 x 10-11; 5,00 x 10-11; 7,27 x 10-11 e 9,14 x 10-11 m2 s-1, para as temperaturas de 35, 45, 60, 75 e 90 °C, respectivamente, durante a secagem dos frutos de crambe. A relação entre o coeficiente de difusão efetivo e a temperatura de secagem pode ser descrita pela equação de Arrhenius, que apresenta uma energia de ativação para a difusão líquida na secagem de 20,998 kJ mol-1.

The arm in this work was set several mathematical models to the process of drying the fruits of Crambe abyssinica in different air conditions and to determine the effective diffusion coefficient and obtain the activation energy. The work was performed at the Laboratory of Postharvest Products Vegetables of Instituto Federal de Educação, Ciência e Tecnologia Goiano ­ Câmpus Rio Verde, Goiás, Brasil (IF Goiano - Câmpus Rio Verde). The harvest of crambe (Crambe abyssinica Hochst) cultivar FMS Brilhante was performed manually with a water content of 38.0±1.0 (% d.b.) and dried until the content of 8.0±1.5 (d.b.% ). The drying procedure was conducted under different temperature controlled conditions of 35, 45, 60, 75 and 90 °C and relative humidity of 20.9, 8.7, 6.8, 4.8 and 2.3% respectively. Among the models that presented the best fit to the experimental data, Page was the best fit for the data in the different conditions of the drying air. The effective diffusion coefficient increased with increasing temperature with values of 2.84 x 10-11, 3.22 x 10-11, 5.00 x 10-11, 7.27 x 10-11 and 9.14 x 10-11 m2 s-1, at temperatures of 35, 45, 60, 75 and 90 °C, respectively, during the drying of crambe fruits. The relation between the effective diffusion coefficient and the drying temperature may be described by the Arrhenius equation, which has an activation energy for the liquid diffusion in drying of 20.998 kJ mol-1.

Detection of induced mutations in CaFAD2 genes by next-generation sequencing leading to the production of improved oil composition in Crambe abyssinica.

Crambe abyssinica is a hexaploid oil crop for industrial applications. An increase of erucic acid (C22:1) and reduction of polyunsaturated fatty acid (PUFA) contents in crambe oil is a valuable improvement. An increase in oleic acid (C18:1), a reduction in PUFA and possibly an increase in C22:1 can be obtained by down-regulating the expression of fatty acid desaturase2 genes (CaFAD2), which code for the enzyme that converts C18:1 into C18:2. We conducted EMS-mutagenesis in crambe, followed by Illumina sequencing, to screen mutations in three expressed CaFAD2 genes. Two novel analysis strategies were used to detect mutation sites. In the first strategy, mutation detection targeted specific sequence motifs. In the second strategy, every nucleotide position in a CaFAD2 fragment was tested for the presence of mutations. Seventeen novel mutations were detected in 1100 one-dimensional pools (11 000 individuals) in three expressed CaFAD2 genes, including non-sense mutations and mis-sense mutations in CaFAD2-C1, -C2 and -C3. The homozygous non-sense mutants for CaFAD2-C3 resulted in a 25% higher content of C18:1 and 25% lower content of PUFA compared to the wild type. The mis-sense mutations only led to small changes in oil composition. Concluding, targeted mutation detection using NGS in a polyploid was successfully applied and it was found that a non-sense mutation in even a single CaFAD2 gene can lead to changes in crambe oil composition. Stacking the mutations in different CaFAD2 may gain additional changes in C18:1 and PUFA contents.