Association between dental age and malocclusions: a systematic review.

BACKGROUND: The evidence in the literature suggests that some skeletal or dental malocclusions are involved with dental development, resulting in advanced or delayed dental age (DA). The purpose of this systematic review was to investigate the association between DA and different types of malocclusions. METHODS: The search was carried out on PubMed, Scopus, Web of Science, Virtual Health Library, and in the gray literature. Observational studies that evaluated the association between DA and sagittal, vertical, or transversal malocclusions were included. The quality assessment was performed using the Newcastle-Ottawa Scale (NOS). The data from primary studies were narratively synthesized. The certainty of evidence was evaluated using the GRADE approach. The study was conducted from August 2023 to October 2023. RESULTS: One Thousand Nine Hundred Ninety-One records were identified in the initial search. Twenty (n = 20) studies were included. Most of the studies (n=15) presented a moderate quality according to NOS. Twelve studies evaluated the association between DA and sagittal discrepancies; eight studies evaluated vertical discrepancies, and only one study analyzed a transversal discrepancy. Demirjian's method for DA assessment was the most used among the studies. The primary studies observed that patients of both sexes presenting a vertical growth pattern and males with skeletal Class III malocclusion tend to have advanced DA. The study that investigated transversal malocclusion found that unilateral posterior cross-bite is associated with delayed DA. The certainty of evidence was very low for all outcomes evaluated. CONCLUSION: DA may be associated with the type of malocclusion. It is suggested that DA can be used as an initial diagnostic tool in orthodontics. Future well-designed studies should be performed in order to investigate the association between DA and different types of malocclusions in more detail. TRIAL REGISTRATION: This study was registered in the PROSPERO database (CRD42023454207).

Impact of genetic variations in the WNT family members and RUNX2 on dental and skeletal maturation: a cross-sectional study.

BACKGROUND: This study evaluated if genetic variations in the WNT family members and RUNX2 are associated with craniofacial maturation, investigating dental and skeletal maturity in children and teenagers. METHODS: Radiographs from pre-orthodontic treatment of Brazilian patients (7 to 17 years-old) were used to assess dental (panoramic radiographs) and skeletal maturity (cephalometric radiographs). The chronological age (CA) was calculated based on the date of birth and the time the radiographs were performed. For the dental maturity analysis, the Demirjian (1973) method was used and a delta [dental age - chronological age (DA-CA)] was calculated. For the skeletal maturity analysis, the Baccetti et al. (2005) method was used and the patients were classified as "delayed skeletal maturation", "advanced skeletal maturation" or "normal skeletal maturation". DNA isolated from buccal cells was used for genotyping of two genetic variations in WNT family genes: rs708111 (G > A) in WNT3A and rs1533767 (G > A) in WNT11; and two genetic variations in RUNX2: rs1200425 (G > A) and rs59983488 (G > T). A statistical analysis was performed and values of p < 0.05 indicated a significant difference. RESULTS: There were no associations between dental maturity and genotypes (p > 0.05). In the skeletal maturity analysis, the allele A in the rs708111 (WNT3A) was statistically more frequent in patients with delayed skeletal maturation (Prevalence Ratio = 1.6; 95% Confidence Interval = 1.00 to 2.54; p-value = 0.042). CONCLUSIONS: The rs708111 in the WNT3A gene impacts on skeletal maturation.

Microcephaly as a risk factor for dental alterations: A case-control study.

OBJECTIVE: This study aimed to assess whether microcephaly is a risk factor for alterations in the chronology and sequence of tooth eruption and for developmental defects of enamel. MATHERIALS AND METHODS: In this case-control study, 81 children aged 30-36 months, including 40 normoreactive children and 41 with microcephaly, were submitted to oral clinical examination to determine the frequency of alterations in the chronology and sequence of tooth eruption and developmental enamel defects. The sample was matched for sex and age (1:1) and allocated to the case (presence of dental alterations) and control (absence of dental alterations) groups. Gestational age, birthweight and socioeconomic characteristics were also analyzed. Chi-square test and Fisher's exact test were applied (α = 0.05). RESULTS: Microcephaly was significantly associated with delayed tooth eruption, alterations in the sequence of tooth eruption, and defects in dental enamel (p < 0.001). Low birthweight also showed a significant association with this alterations (p < 0.005) and prematurity was associated with defects in enamel development (p < 0.005). CONCLUSION: Microcephaly is a risk factor for alterations in the tooth eruption process and enamel formation in primary teeth.

A large sample-sized study on dental development of children treated at the Central Dental Clinic (OCEx) of the Brazilian Army.

OBJECTIVES: To test and compare the performance of two radiographic methods for dental age estimation on a large sample of Brazilian boys and girls. MATERIAL AND METHODS: The sample consisted of 1.990 panoramic radiographs of Brazilian children (age: 3-15.9 years) equally balanced based on sex. The sample was distributed into ten age categories, each with up to 200 children. Age estimation was performed with Willems' (2001) and Demirjian's (1973) methods. Estimated (EA) and chronological (CA) ages were compared. The performances of the methods were quantified based on sex and age category. RESULTS: The overall differences between CA and EA for Willems' method in boys and girls were 0.06 and - 0.02, respectively. For Demirjian's method, the differences were 0.60 and 0.74, respectively. The overestimations of Demirjian's method were statistically significant (p < 0.001). Willems' method reached the best outcomes among children between 3 and 12 years, while Demirjian's best performances were between the ages of 7 and 10 years. CONCLUSION: Willems' method led to differences between CA and AE that were acceptable for clinical and forensic practice. CLINICAL RELEVANCE: Age estimation may guide clinical decisions based on treatment timing. Validating international tools is necessary to promote evidence-based practice and country-specific application. This study overcame the limitations of previous research to provide a more realistic perspective of the performance of age estimation methods in Brazilian children. Willems' method had a superior performance compared to Demirjian's method and led to outcomes that were better than most studies with the Brazilian population.

Testing the Olze and Timme Methods for Dental Age Estimation in Radiographs of Brazilian Subadults and Adults.

AIM: This study aimed to test the applicability of the methods proposed by Olze et al. (2012) and Timme et al. for dental age estimation in Brazilian subadults and adults. MATERIAL AND METHODS: Panoramic radiographs of 503 individuals aged between 20 and 70 years were analyzed. The mean chronological age of males and females of the sample was 29.04 and 29.97 years, receptively. Secondary dentin formation, cementum apposition, periodontal recession, and attrition of teeth #34, #35, #44 and #45 teeth were assessed as parameters to be calculated in the formulae designed by Olze et al. and Timme et al. RESULTS: In males, the mean estimated age by the Olze method was 28.97 ± 2.86 years, while in females it was 27.85 ± 2.70 years. The Timme method estimated mean age for males of 32.54 ± 5.32 years and for females 33.09 ± 5.16 years. Low coefficients of determination (r2) for the application of tooth-specific formulae of both methods suggest that estimated and chronological ages were not properly associated. CONCLUSION: The outcomes of this study indicate that the Olze and Timme methods may be limited for forensic applications in Brazilian subadults and adults. Country-specific statistical adjustments might be useful for improvements in practice.

Human permanent tooth sizes are associated with genes encoding oestrogen receptors.

OBJECTIVE: To evaluate if genetic polymorphisms in the oestrogen receptor 1 (ESR1) and oestrogen receptor 2 (ESR2) genes encoded for oestrogen receptors alpha (ERα) and beta (ERß) are involved in permanent tooth size. DESIGN: Cross-sectional study. SETTING: Orthodontic Clinic at School of Dentistry of Ribeirão Preto, University of São Paulo. PARTICIPANTS: A total of 108 orthodontic patients. MATERIALS AND METHODS: Pre-treatment orthodontic records were evaluated. Dental casts were used to determine the maximum crown measurements of fully erupted permanent teeth in the mesiodistal dimensions. Second and third molars were not included in the analysis. Genomic DNA samples were used for the genotyping of four genetic polymorphisms: ESR1 (rs9340799 and rs2234693) and ESR2 (rs1256049 and rs4986938). The associations between tooth size and sex were evaluated using t test. The associations between tooth size and genotype were analysed with linear regression and adjusted by sex at an alpha of P⩽0.05. RESULTS: Female patients presented smaller tooth size than male patients. A statistically significant difference was observed in almost all teeth (P<0.05). The genetic polymorphisms in rs9340799, rs2234693, rs1256049 and rs4986938 were associated with some tooth sizes in both the maxilla and mandible (P<0.05). CONCLUSION: This study provides evidence that genetic polymorphisms in ESR1 and ESR2 could be associated with tooth size in permanent teeth.

Genetic variants in tooth agenesis-related genes might be also involved in tooth size variations.

OBJECTIVE: The present study aimed to evaluate if genetic variants in PAX9, MSX1, TGFα, FGF3, FGF10, FGF13, GLI2 and GLI3 are involved in TS of permanent teeth. MATERIALS AND METHODS: Pretreatment dental records from orthodontic patients were assessed prior to recruitment. Patients with tooth agenesis and congenital anomalies (including oral cleft) and/or syndromes were excluded. Dental casts were used to measure the maximum crown dimensions of all fully erupted permanent teeth except second and third molars in mesiodistal direction. Teeth with caries, occlusal wear, mesiodistal restorations, and obvious deformities were not evaluated. Genomic DNA samples were used for genotyping. The allelic discrimination of 13 genetic variants was performed. The associations between TS and genotype were analyzed by linear regression, adjusted by gender at a significance level of p ≤ 0.05. RESULTS: Genetic polymorphisms in the tooth agenesis-related genes studied here were associated with increased and decreased TS, in both maxilla and mandible (p < 0.05). CONCLUSION: This study reported associations of novel tooth agenesis-related gene variants with permanent tooth size variations. CLINICAL RELEVANCE: The presence of some genetic variants could allow the prediction of permanent tooth size.

Correlação entre maturidade dentária e índice de massa corporal em pacientes com má oclusão de Classe II / Correlation between dental maturity and body mass index in Angle Class II patients

Resumo Objetivo: Avaliar a correlação entre o Índice de Massa Corporal (IMC) e o desenvolvimento dentário em pacientes portadores de má oclusão de Classe II. Materiais e Métodos: Tratou-se de um estudo retrospectivo com uma amostra de 37 pacientes portadores de má oclusão de Classe II, que estavam em tratamento ortodôntico. Os dados idade, gênero, peso e altura foram coletados dos prontuários, juntamente com a tomada tomográfica inicial. O desenvolvimento dentário foi avaliado pelo método de Demirjian et al. (1973). O coeficiente de correlação de Pearson foi utilizado para determinar a força de correlação entre as variáveis. A significância estatística considerada foi p <0,05 bicaudal. Resultados: A amostra foi constituída por pacientes entre 10 e 16 anos. A maioria dos pacientes (78,4%) apresentou IMC dentro da normalidade. Observou-se correlação fraca sem significância estatística entre o desenvolvimento dentário e o IMC nos pacientes avaliados (r=-0,10; p=0,55). Conclusão: Não houve correlação entre o IMC e o desenvolvimento dentário para a amostra estudada (AU)

Abstract Objective: To evaluate the correlation between the Body Mass Index (BMI) and dental development in patients with Class II malocclusion. Materials and Methods: This was a retrospective study with a sample of 37 patients presenting Class II malocclusion, who were undergoing orthodontic treatment. The age, gender, weight and height data were collected from the medical records, together with the initial CT scan. Dental development was assessed using the method of Demirjian et al. (1973). Pearson's correlation coefficient was used to determine the correlation strength between variables. The statistical significance considered was p <0.05 two-tailed. Results: The sample consisted of patients between 10 and 16 years old. The majority of the patients (78.4%) had a normal BMI. There was a weak correlation without statistical significance between tooth development and BMI in the evaluated patients (r=-0.10; p=0.55). Conclusion: There was no correlation between BMI and dental development for the sample studied.(AU)

Gestational protein restriction alters the RANKL/OPG system in the dental germ of offsprings.

Evaluate the dentinogenesis in the offspring of rats submitted to gestational protein restriction (GPR). DESIGN: The offspring were evaluated at the 21st day of gestation (21 dG). Assessments were made of morphological parameters and the RANKL/OPG system - bone tissue maturation markers - in the upper incisor tooth germ. Pregnant 10-week-old female Wistar rats were divided into normal protein (NP, 17% casein, n = 5) and low protein (LP, 6% casein, n = 5) diet groups. At 21 dG, the offspring maxillae were collected for histomorphometric and immunohistochemical analyses. RESULTS: The LP group showed decreased thickness of the dentin and odontoblast cell layers on the tooth germ. GPR led to decreased OPG expression and increased RANKL expression in the incisor germ. CONCLUSION: The results suggested that gestational protein restriction altered odontoblast RANKL/OPG expression and decreased dentin matrix deposition and thickness in tooth development.

Relationship between cervical vertebral maturation and dental development in a sample of yemeni children and adolescents / Relación entre la maduración vertebral cervical y el desarrollo dental en una muestra de niños y adolescentes yemeníes

Objective: This study was conducted to investigate the relationship between dental development and cervical vertebral maturation stages in a group of Yemeni children and adolescents. Materials an Methods: The study included digital panoramic radiographs and lateral skull cephalograms obtained from 207 Yemeni subjects­122 females and 85 males aged between 8 to 18 years. Dental maturity was evaluated according to the method of Demirijian et al., calcification stages of the left mandibular canines, first and second premolars and second molars were assessed. Skeletal maturity was assessed by the cervical vertebral maturation (CVM) stages according to the method of Baccetti et al. Correlation between CVM and dental maturation was evaluated by Spearman rank-order correlation coefficient (SROCC). Results: CVM and dental calcification stages were highly correlated (p<0.001) in both genders, ranging from 0.686 to 0.873 for females and 0.787 to 0.871 for males. Calcification stages of the second molars showed the strongest correlation with CVM. Conclusion: Calcification stages of the second molar may be used as a reliable maturation indicator. Dental maturation may be applied to determine the skeletal maturity status of Yemeni children and adolescents.

Objetivo: Este estudio se realizó para investigar la relación entre el desarrollo dental y las etapas de maduración vertebral cervical en un grupo de niños y adolescentes yemeníes. Material y Métodos: El estudio incluyó radiografías panorámicas digitales y cefalogramas laterales del cráneo obtenidos de 207 sujetos yemeníes: 122 mujeres y 85 hombres de entre 8 y 18 años. La madurez dental se evaluó de acuerdo con el método de Demirijian et al. Se evaluaron las etapas de calcificación de los caninos mandibulares izquierdos, primer y segundo premolares y segundos molares. La madurez esquelética se evaluó mediante las etapas de maduración vertebral cervical (CVM) de acuerdo con el método de Baccetti et al. La correlación entre la CVM y la maduración dental se evaluó mediante el coeficiente de correlación de orden de rango de Spearman (SROCC). Resultado: Las etapas de CVM y calcificación dental estuvieron altamente correlacionadas (p<0.001) en ambos sexos, con un rango de 0.686 a 0.873 para las mujeres y 0.787 a 0.871 para los hombres. Las etapas de calcificación de los segundos molares mostraron la correlación más fuerte con CVM. Conclusión: las etapas de calcificación del segundo molar pueden usarse como un indicador de maduración confiable. La maduración dental puede aplicarse para determinar el estado de madurez esquelética de los niños y adolescentes yemeníes.

Comparación de los métodos de Nolla, Demirjian y Moorrees en la estimación de la edad dental con fines forenses / Comparison of Nolla, Demirjian and Moorrees methods for dental age calculation for forensic purposes

RESUMEN: Objetivo: El propósito de este estudio fue comparar tres métodos de estimación de la edad dental (ED) con fines forenses. Material y métodos: Se seleccionaron 512 radiografías panorámicas de sujetos de Maracaibo, estado Zulia, Venezuela, de ambos sexos (272 hembras y 240 varones), con edades cronológicas (EC) entre 6-18 años. Se asignaron los estadios de maduración propuestos por Nolla, Moorrees et al y Demirjian et al a siete dientes mandibulares permanentes del lado izquierdo, la ED fue calculada de acuerdo con la metodología de cada autor. Se obtuvo la EC en la cual se observaron los diferentes estadios de maduración, así como las diferencias de media entre la EC y la ED estimada por cada método mediante un test de Student para muestras relacionadas. Resultados: En general, las hembras alcanzaron los estadios de maduración a edades más tempranas que los varones. Se evidenció en el total de la muestra, una sobreestimación de la edad para el método de Demirjian et al (-0.14 ± 1.45), mientras que para el de Nolla y Moorrees et al se observó una subestimación, esta subestimación fue mayor para el método de Moorrees et al (2.63 ± 2.09) que para el de Nolla (0.42 ± 1.38), siendo que las diferencias encontradas entre la EC y la ED fueron estadísticamente significativas. Conclusión: Se determinó que para el total de la muestra, el método de Demirjian et al fue el más preciso.

ABSTRACT: Objective: The purpose of the present study was to compare three methods for calculation of dental age (DA) to be used for forensic purposes. Material and methods: 512 panoramic X-rays of subjects of both genders living in Maracaibo, State of Zulia, Venezuela were selected (272 females, 240 males). Selected subjects were in the 6-18 years chronological age (CA) range. Maturation stages of Nolla, Moorrees et al and Demirjian et al were assigned to seven permanent teeth of the left side, and DA was calculated according to methodology of each author. CA was obtained where different stages of maturation were observed, as well as mean difference between DA and CA as calculated with each method were obtained with a t student test for related samples. Results: In general, females reached maturation stages at earlier ages than males. The total sample revealed age over-estimation for the Demirjian method (-0.14 ± 1.45), whereas, a sub-calculation was observed for the Nolla and Moorrees et al method. This under-estimation was greater for the Moorrees at al method (2.63 ± 2.09) when compared to Nolla method (0.42 ± 1.38) and differences between DA and CA were found to be statistically significant. Conclusion: In the total studied sample, it was determined that Demirjian et al method was the most accurate.

Dentinogenesis Imperfecta: A Case Report of Five Patients in the Same Family Group / Dentinogenesis Imperfecta: Un Reporte de Caso de Cinco Pacientes en el Mismo Grupo Familiar

Dentinogenesis imperfecta (DI) is a type of dentin dysplasia that affects the dentin structure of one or both dentitions, which may be classified in three types. The aim of this report was to show the clinical and radiographic features of the four cases of DI in the same family group. Five brothers were checked clinically and radiographically. Two individuals were diagnosed, by their phenotypic features and medical history, with DI type I; two of them with DI type II and one case without signs of DI. It is important to know the features of dentinogenesis imperfecta to perform a comprehensive dental care, including the right diagnosis and an effective treatment plan.

La dentinogénesis imperfecta (DI) es un tipo de displasia de la dentina que afecta su estructura en una o ambas denticiones. La DI puede clasificarse en tres tipos. El objetivo de este informe fue demostrar las características clínicas y radiológicas de los cuatro casos de DI en un mismo grupo familiar. Cinco hermanos fueron controlados clínica y radiográficamente. Dos individuos fueron diagnosticados, por sus características fenotípicas y antecedentes clínicos, con el tipo de DI I; dos de ellos con DI de tipo II y un caso sin signos de DI. Es importante conocer las características de la dentinogénesis imperfecta para poder realizar una atención odontológica integral, lo que permitirá desarrollar un diagnóstico correcto y un plan de tratamiento efectivo.

Correlating skeletal and dental developmental stages using radiographic parameters.

The demand for age estimation of living and deceased children considerably increased in the last years. It was mainly justified by the growing globalization trend and the enlarged amount of violent crimes involving young victims. The present research aims to correlate skeletal and dental developmental stages using radiographic parameters. Lateral cephalometric (n = 576) and panoramic (n = 576) radiographs taken in the same moment were used to asses skeletal and dental development according to the methods of Hassel and Farman (1995) and Bacetti et al. (2002), and Demirjian et al. (1973), respectively. Likelihood-ratio test was used to verify the positive prediction in correlations between developmental stages. Spearman's correlation coefficient was calculated between skeletal and dental developmental stages, as well between estimated and chronological ages. Higher prediction in correlation for the skeletal starting stage (stage 1) with stage E in mandibular canines, and with stage D in mandibular premolars and second molars was detected. Higher staging correlations were observed considering the mandibular left second premolar (0.652, p < 0.001 for the technique of Bacetti et al.; and 0.646, p < 0.001 for the technique of Hassel and Farman). Despite the correlations between skeletal and dental developmental stages, the results must be carefully interpreted once it reached moderate values (<0.652). Further researches must test different classifications of skeletal and dental development, in order to verify the possibility of replacing one technique for another with stronger correlation.

Anestesia local odontológica y su influencia en anomalías del desarrollo dental: revisión sistemática de la literatura / Dental local anesthesia and its influence on dental developmental abnormalities: systematic review of the literature

La anestesia local odontológica es un procedimiento de rutina en la práctica odontológica pediátrica que, tal como otros sucesos, podría generar anomalías en el desarrollo dental, considerando la susceptibilidad de los gérmenes dentales en desarrollo; por lo anterior consideramos necesario determinar la existencia de asociación entre anomalías del desarrollo dental y el uso de técnicas anestésicas. Para esto se diseñó un estudio descriptivo basado en búsqueda sistemática de literatura en las bases de datos: Scopus, MEDLINE, Web of Science Core Collection, ProQuest Central, Korean Journal Database y SciELO, utilizando combinación de términos MeSH: ("odontoblasts" OR "ameloblasts" OR "odontogenesis" OR "amelogenesis" OR "dentinogenesis" OR "odontodysplasia" OR "dental enamel hypoplasia" OR "dental sac" OR "dental papilla" OR "enamel organ") AND ("anesthesia" OR "local anesthesia" OR "nerve block" OR "dental anesthesia"). De un total de 96 artículos, se incluyeron para revisión 5 artículos relacionados con los objetivos de esta revisión, excluyéndose las coincidencias. La evidencia presente en la literatura es contradictoria. Existen múltiples diferencias entre los estudios analizados, las que podrían deberse a las técnicas anestésicas involucradas, tipo de anestésico, la no consideración de otros posibles agentes causales (síndromes) o la terapia odontológica. No puede ser determinado una relación entre estos dos aspectos.

The dental local anesthesia is a routine procedure in pediatric dental practice that, as other events could generate abnormalities in dental development, considering the susceptibility of developing tooth germs. From the foregoing it necessary to determine the existence of association between disorders of tooth development and use of anesthetic techniques. A descriptive study based on systematic literature review in databases: Scopus, MEDLINE, Web of Science Core Collection, ProQuest Central, Korean Journal Database and SciELO data was designed, using the following combination of MeSH terms ("odontoblasts" OR "ameloblasts" OR "odontogenesis" OR "amelogenesis" OR "dentinogenesis" OR "odontodysplasia" OR "dental enamel hypoplasia" OR "dental sac" OR "dental papilla" OR "enamel organ") AND ("anesthesia" OR "local anesthesia "OR" nerve block "OR" dental anesthesia"). A total of 96 articles, were included for review a total of 5 articles related to the objectives of this review, excluding the matches. The present evidence in the literature is contradictory. There are many differences between the studies analyzed, which could be due to the anesthetic techniques involved, type of anesthetic, other possible causative agents (syndromes) or dental treatment are not included. It can not be determined a relationship between these two aspects.

Long-term dental follow-up in hematological stem cells transplantation children / Acompanhametnto a longo prazo das alterações dentárias em pacientes pediátricos submetidos ao transplante de celulas tronco hematopoieticas

The aim of this paper is to update the current published content about the craniofacial long-term development disturbances of childhood hematopoietic stem-cell transplantation (HSCT) and the preparative regimens for the onco-hematological malignancies treatments. Methods: Two author sindependently reviewed the published articles about long-term effects of childhood HSCT that fitted into predetermined inclusion/exclusion criteria: clear definition of exposure or intervention, standard outcomes measurement and appropriate statistical analysis. Results: Twelve papers matched all the previous established eligibility criteria and were included in this review. The children’s age at HSCT were related to a higher risk of dental development disturbances, such as agenesis, dental hypoplasia, root stunting, crown-root proportion alterations, and microdontia. Craniofacial vertical growth was impaired in the irradiated patients without antero-posterior or latero-lateral impairment. Temporomandibular joint dysfunction was found to be more prevalent in the patients diagnosed withgraft-versus-host disease. Conclusion: The late effects of craniofacial development disturbances lead to several aesthetic and functional impairment, periodontal bone resorption with consequent impairment of chewing ability, and risk of early tooth loss often associated with life’s quality impairment. Further investigations should be performed to provide accurate information for patients, parent sand health care professionals...

Este artigo visa revisar o conteúdo publicado sobre as alterações tardias em crianças submetidas ao transplante de células-tronco hematopoiéticas(TCTH) e o regime pré-transplante para tratamento das doenças onco-hematológicas. Método: Dois autores independentes conduziram uma revisão simples dos artigos recentemente publicados sobre o tema, utilizando os critérios de exclusão/inclusão pré determinados: definição clara da intervenção clínica realizada, mensuração dos resultados padronizada e análise estatística adequada. Resultados: Doze artigos enquadraram-se em todos os critérios de avaliação pré-determinados para esta revisão. Houve correlação entre a idade da criança no momento do TCTH e maior risco de distúrbios de desenvolvimento dentário, como agenesia, hipoplasia dentária, encurtamento radicular,alterações da proporção coroa-raiz e microdontia.Houve alteração do crescimento craniofacial no sentido vertical sem, no entanto, alterar o crescimento anteroposterior e latero-lateral. Disfunção da articulação temporomandibular foi mais prevalente entre os pacientes com diagnostico de Doença do Enxerto contra o Hospedeiro. Conclusão: Os efeitos tardios do desenvolvimento craniofacial podem levara diversas alterações estéticas e funcionais, reabsorção óssea periodontal, diminuição da capacidade de mastigação e perda dentária precoce com possíveis impactos negativos sobre a qualidade de vida. Mais estudos são necessários para fornecer informações precisas aos pacientes, cuidadores e profissionais de saúde...

Estimación de Edad Dentaria Utilizando el Método de Demirjian en Niños de 5 a 15 Años de Curicó, Chile / Dental Age Estimation Using the Demirjian's Method in Children 5 to 15 Years of Age in Curicó, Chile

El método de Demirjian (MD) es el más ampliamente difundido para estimar la edad dental (ED). Con este método se han realizado numerosos estudios en diferentes grupos étnicos, analizándose niños europeos, asiáticos y norteamericanos entre otros. Estos resultados sugieren posibles diferencias en los patrones de maduración dental entre las diferentes poblaciones. El objetivo del presente estudio fue determinar la edad dentaria aplicando el MD en una muestra de 363 ortopantomografías de pacientes entre 5 y 15 años, obtenidas de un centro radiológico maxilofacial privado en Curicó, VII región, Chile. Se obtuvo un coeficiente de correlación inter-clase (CCI), como medida de correlación entre edad dental y cronológica, de 0,974 (CCI 95% 0,968­0,979). El CCI para niños fue de 0,972 (CCI 95% 0,963- 0,979) y en el caso de las niñas fue de 0,977 (CCI 95% 0,969­0,983). En general, el método de Demirjian tendió a sobreestimar la edad en los niños y niñas. La ED observada fue mayor que la edad cronológica (EC) en 130 niños y en 138 niñas, resultó igual a la EC en 13 niños y 8 niñas, y fue menor que la EC en 38 niños y 33 niñas. En conclusión, nuestros resultados coinciden con hallazgos en numerosos lugares del mundo, es decir, la ED en este grupo se fue levemente mayor que la muestra original franco-canadiense. Sin embargo, el rango obtenido de la ED es similar al de la EC y el grado de correlación entre ambas es casi perfecto al aplicar el CCI. El grado de correlación entre ambas edades para cada sexo también es muy bueno. Por lo tanto, consideramos que el MD es aplicable a esta muestra de niños.

The Demirjian's method (DM) is the most widely used method for estimating dental age. A number of studies with this method have been performed in different ethnic groups, with European, Asian y North American children being analyzed among others. Possible differences in patterns of dental maturation among different population have been suggested. The aim of this study was to determine dental age with the DM in a sample of 363 panoramic radiographs from children 5-15 years old, obtained from a private radiological maxillofacial center in Curicó, Chile. An inter-class correlation coefficient (ICC) was obtained as a measure of correlation between dental and chronological age of 0.974 (ICC 95% 0.968-0.979). The ICC for boys was 0.972 (ICC 95% 0.963- 0.979) and 0.977 for girls (CCI 95% 0.969-0.983). In general, DM overestimated the age in girls and boys. Dental age (DA) was observed to be higher than chronological age (CA) in 130 boys and 138 girls; DA was equal to CA in 13 boys y 8 girls and lower than CE in 38 boys and 33 girls. In conclusion, our study concurs with several other studies in other places of the world, indeed the DA was found to be higher when compared to the original French-Canadian sample. However, the range obtained for DA is similar to CA and the degree of correlation between both is almost perfect when applying ICC. The degree of correlation between both ages for each sex was found as very good. Furthermore, we consider that DM can be applied to this sample of children.

Discordant tooth agenesis and peg-shaped in a pair of monozygotic twins: Clinical and molecular study.

The present report aimed to study an uncommon case of a pair of twins that presented a discordant dental phenotype. Family investigation, clinical, radiographic examination and molecular analysis were performed in both girls. Molecular analysis confirmed the monozygosity by deoxyribonucleic acid chip technology. One twin presented tooth agenesis in left upper lateral incisor and peg-shaped on the contra lateral side while the other twin had no dental alterations. The dental casts study employed digital caliper to compare morphological dimensions and showed alteration only in peg-shaped tooth. In conclusion, this study provide support that one or more mutated gene could cause discordances in dental phenotype in these monozygotic twins.

Regional odontodysplasia: a case report / Odontodisplasia regional: relato de caso clínico

PURPOSE: Regional odontodysplasia (RO) is a rare condition characterized by distinctive clinical, radiographic and microscopic findings. It is presumed to be the result of a developmental disturbance that locally affects the odontogenic ectodermal and mesodermal tissues. This report describes the clinical and radiographic findings of RO and its treatment. CASE DESCRIPTION: A 15-year-old boy presented with an uncommon case of RO involving two quadrants of the jaws on the right side of his face. CONCLUSION: A complex multidisciplinary team is required to fully rehabilitate a patient affected by RO. The treatment of RO is controversial and varies according to the individual patient.

OBJETIVO: Odontodisplasia regional (OR) é uma condição rara caracterizada por achados clínicos, radiográficos e microscópicos bem distintos. É o resultado de um distúrbio de desenvolvimento bem localizado afetando tanto o ectoderma quanto o mesoderma odontogênico. Este trabalho descreveu os achados clínicos e radiográficos de um caso de OR e os tratamentos. DESCRIÇÃO DO CASO: Um menino de 15 anos de idade apresentou um caso incomum de OR envolvendo dois quadrantes dos maxilares no lado direito da face. CONCLUSÃO: Há necessidade de uma equipe multidisciplinar complexa para reabilitar inteiramente um paciente com OR. O tratamento de OR é controverso e varia de acordo com as características e resposta do paciente.

Pseudomonas aeruginosa in the Early Childhood: A Case Report.

Pseudomonas aeruginosa is an opportunistic bacterium that usually affects immunocompromised patients, causing infections whose signals and symptoms are related to the affected organ. The patient presented in this article was infected when he was 9 months old. Such condition led to certain alterations like dental improperly positioned teeth, retained deciduous teeth, hipodonty of permanent teeth, atrophy of the upper jaw and dental crowding. Therefore, the purpose of this article is to report the case of a patient affected by Pseudomonas aeruginosa infection in the early childhood and to describe the dental development disorders as consequence of this fact.

Dental development of Didelphis albiventris (Marsupialia): I - incisors and canines

The formation of incisors and canines in marsupials of D. albiventris was studied at various stages of development. Seventy-six specimens, with ages varying from 0 to 100 days, were used in this investigation. Serial sections of the maxilla were obtained in the transverse plane and stained with hematoxylin and eosin. Histological analyses were made to verify the pattern of teeth development, as well as their chronology of eruption. The period of time from birth to 100 days comprised the entire process of teeth development, from epithelial bud formation to early eruption of the teeth. Oral epithelium thickening gave rise to the functional incisors and canines. In addition, a secondary dental lamina emerged in different phases of development in the outer epithelium of incisors and canines, which degenerated when it reached the bud stage. No evidence of deciduous dentition was observed. The results of this investigation suggest that secondary dental lamina represents remnants of a primitive condition in which secondary dentition used to be present.

Estudou-se o desenvolvimento dos dentes incisivos e caninos em 76 amostras de Didelphis albiventris com idade entre 0 e 100 dias. Cortes transversais, seriados de 6 µm de espessura foram obtidos da região da maxila, corados com Hematoxilina e Eosina e analisados ao microscópio de luz. Verificou-se que o período estudado abrange todo o desenvolvimento dental, desde a fase de iniciação da interação epitélio/mesenquima até a completa formação e erupção dos incisivos e caninos. O espessamento do epitélio oral dá origem aos incisivos e caninos funcionais, enquanto o epitélio dental externo do órgão dental origina uma lâmina dental secundária, a qual sofre degeneração, quando o dente alcança o estágio de botão. Não há vestígios de dentição decídua. Sugere-se que a lâmina dental secundária é remanescente de uma condição primitiva na qual ocorria dentição secundária.