Efficacy of 0.2% hyaluronic acid in the healing of skin abrasions in rats.

Acute injuries, such as surgical and traumatic, heal normally in an organized and rapid manner. Studies point to the healing activity of hyaluronic acid in all phases of healing. The aim was to evaluate the effectiveness of hyaluronic acid in skin abrasions on the dorsum of rats to compare to usual products on the market. Seventy-two Wistar rats were subjected to excoriation of approximately 2.0 cm2 on the back by dermabrasion. According to the treatment, 3 groups were established: saline, chlorhexidine digluconate and 0.2% hyaluronic acid for 14 days. Animals were photographed on the 2nd, 7th, 10th and 14th postinjury days, and the index of healing of the abrasions was calculated. Biochemically, myeloperoxidase measurements of skin biopsies in addition to histological studies to assess the crust and epidermal layers were performed. The group treated with hyaluronic acid showed better re-epithelialization from the other groups (p < 0.05) on the 7th and 10th days. For the thickness of the crust, the hyaluronic acid group presented thinner crust than other groups on the 10th and 14th days (p < 0.05), but in the epidermis, no difference was observed between the groups studied. All groups showed an increase in myeloperoxidase enzyme on the 2nd day, but a decreasing on the 7th day. On the 10th day, there was a difference in the hyaluronic acid group compared to the other groups (p < 0.05). The application of 0.2% hyaluronic acid significantly accelerated the re-epithelialization of skin abrasions compared to saline and chlorhexidine digluconate.

Micro needling: A novel therapeutic approach for androgenetic alopecia, A Review of Literature.

Androgenetic alopecia (AGA) is an androgen-dependent hereditary trait resulting in hair miniaturization. It is the most common type of alopecia in men and women. During the last years, multiple treatment modalities have been studied, but only topical minoxidil and finasteride have been approved by the US Food and Drug Administration. Microneedling (MN) is a minimally invasive technique that induces collagen formation, as well as growth factors production and neovascularization. Even though not many studies of MN in alopecia have been performed, it remains a favorable treatment modality; however, no standardized protocol for MN in hair loss has been proposed yet. Current evidence is not sufficient to allow a direct comparison with other therapies, but it shows promises to increase hair density, thickness, and quality of hair, especially when combined with other treatments or when used as a drug delivery system. This article aims to summarize the available literature regarding the use of MN alone or associated with other therapies for the treatment of androgenetic alopecia.

Healing effects of natural latex serum 1% from Hevea brasiliensis in an experimental skin abrasion wound model

Abstract Background Dermabrasion is related with mechanical and surgical traumas on the skin; usually topical antiseptics and/or saline have been used for healing. Natural products for wound healing can also be used for abrasions, such as latex from Hevea brasiliensis. Objective This study aimed to evaluate the in vitro viability and migratory/proliferative effects of latex serum from H. brasiliensis and to compare with a commercially available standard antiseptic solution and saline in experimental dermabrasion on rats. Methods For in vitro evaluation, MTT and scratch assays were used. In vivo testing was performed in 72 rats submitted to dermabrasion, treated with saline, antiseptic, or latex serum. This study evaluated re-epithelialization, neutrophilic infiltration, and the quantification of crust and epidermis. Results Latex showed viability at 1% and 0.1% concentrations and migratory/proliferative activity at 0.01% concentrations. The re-epithelialization was highest in latex group on 7th day. The latex group displayed lower thickness of crusts and greater extent of epidermal layers. The latex and antiseptic groups showed increases of myeloperoxidase levels on the 2nd day and showed important reductions from the 7th day. Study limitations Acute superficial wound model in rats and non-use of gel-cream (medium) without latex. Conclusion In conclusion, non-toxic latex stimulated migration/proliferation of keratinocytes in vitro and significantly accelerated wound healing in animal excoriation models compared to chlorhexidine or saline.

Healing effects of natural latex serum 1% from Hevea brasiliensis in an experimental skin abrasion wound model.

BACKGROUND: Dermabrasion is related with mechanical and surgical traumas on the skin; usually topical antiseptics and/or saline have been used for healing. Natural products for wound healing can also be used for abrasions, such as latex from Hevea brasiliensis. OBJECTIVE: This study aimed to evaluate the in vitro viability and migratory/proliferative effects of latex serum from H. brasiliensis and to compare with a commercially available standard antiseptic solution and saline in experimental dermabrasion on rats. METHODS: For in vitro evaluation, MTT and scratch assays were used. In vivo testing was performed in 72 rats submitted to dermabrasion, treated with saline, antiseptic, or latex serum. This study evaluated re-epithelialization, neutrophilic infiltration, and the quantification of crust and epidermis. RESULTS: Latex showed viability at 1% and 0.1% concentrations and migratory/proliferative activity at 0.01% concentrations. The re-epithelialization was highest in latex group on 7th day. The latex group displayed lower thickness of crusts and greater extent of epidermal layers. The latex and antiseptic groups showed increases of myeloperoxidase levels on the 2nd day and showed important reductions from the 7th day. STUDY LIMITATIONS: Acute superficial wound model in rats and non-use of gel-cream (medium) without latex. CONCLUSION: In conclusion, non-toxic latex stimulated migration/proliferation of keratinocytes in vitro and significantly accelerated wound healing in animal excoriation models compared to chlorhexidine or saline.

Galvano-puncture and dermabrasion for striae distensae: a randomized controlled trial.

OBJECTIVE: To assess the effects of galvano-puncture (GG) and dermabrasion (DG) in reducing striae distensae in the gluteal region of women. METHODS: This randomized, controlled, single-blind clinical trial was conducted at the UNIFAL-MG. Participants were 48 female who had striae distensae alba in the gluteal region. They were randomly divided in GG; DG; and Control Group (CG). The length and width of the largest striae were measured (in millimeters) using a caliper. The same striae were assessed before and after treatment. Infrared thermography was performed in the gluteal region to assess local microcirculation. RESULTS: Intragroup analysis showed a significant reduction in the dimension of the striae between baseline and treatment session 10 in the GG and DG groups. Between-group analysis revealed a reduction in the width and length of the striae for both the GG and DG groups, but there were no significant differences between the two groups. When compared to the CG and the DG group, the GG group had significant improvements, as shown by thermography. CONCLUSION: Both GG and DG are effective in reducing striae length and width. However, only the thermography results showed significant differences between GG and control, and between GG and DG.

Pesquisa de opinião sobre eficácia, custos e cicatrização de procedimentos de resurfacing para rugas estáticas faciais / Expert survey on the efficacy, costs and healing of resurfacing procedures for facial static wrinkles

Foi realizada uma pesquisa de opinião entre especialistas em "resurfacing" cutâneo com a finalidade de compararem-se custos e benefícios entre as técnicas de laser de CO2 totalmente ablativo, lasers fracionados ablativos, lasers fracionados não ablativos, radiofrequência fracionada ablativa, microagulhamento, dermabrasão, peelings médios e peelings de fenol- -óleo de cróton. A opção mais eficaz para tratamento de rugas estáticas faciais foi peeling de fenol-óleo de cróton, ranqueado em primeiro lugar por 71,4% dos experts, seguido de laser de CO2 totalmente ablativo, por 28,6%. Sobre "downtime", os procedimentos elencados com recuperação mais rápida foram microagulhamento e laser fracionado não ablativo, com 42,9% cada um, seguidos por radiofrequência fracionada ablativa, com 14,3%. O período para recuperação completa dos efeitos colaterais e adversos foi percebido como mais curto no microagulhamento por 50% dos experts. Sobre os custos operacionais, o procedimento menos custoso foram peelings médios, de acordo com a opinião de 71,4% dos especialistas. Tais resultados apontam para melhor custo/benefício de procedimentos como peelings químicos e microagulhamento, de acordo com a opinião dos 14 especialistas brasileiros que responderam ao questionário.

A survey on the opinion of specialists regarding skin resurfacing was performed, with the objective of comparing costs and benefits between the techniques: totally ablative CO2 laser, fractional ablative lasers, fractional non-ablative lasers, ablative fractional radiofrequency, microneedling, dermabrasion, medium peels, phenol-croton oil peels. The most effective option for the treatment of static wrinkles was the phenol-croton oil peel, in first place by 71.4% of the experts, followed by totally ablative CO2 laser, by 28.6%. Regarding downtime, procedures ranked as fastest recovery were microneedling and fractional non-ablative lasers, with 42.9% each, followed by ablative fractional radiofrequency, with 14.3%. Complete recovery period for side and adverse effects was perceived as shorter with microneedling by 50% of the experts. Regarding operating costs. The less costly procedure was medium peels, according to the opinion of 71.4% of the experts. Such results point towards a better cost/benefit of procedures such as chemical peels and micro-needling, according to the opinion of 14 Brazilian specialists who answered the questionnaire.

Enxerto por raspagem epidérmica no vitiligo estável: uma opção terapêutica / Grafting by epidermal scraping in stable vitiligo: a therapeutic option

O vitiligo é despigmentação adquirida caracterizada pela perda parcial ou total dos melanócitos da epiderme. Inúmeras modalidades terapêuticas foram propostas para seu tratamento. Nos casos de vitiligo estável os tratamentos cirúrgicos são a preferência. O enxerto por raspagem epidérmica é uma variante da técnica de microenxertos por punch. Constitui técnica nova, simples e de baixo custo, com altas taxas de repigmentação. O objetivo deste relato é demonstrar a experiência dos autores com essa técnica e discutir os resultados obtidos numa série inicial de dois casos (três lesões).

Vitiligo is an acquired depigmentation characterized by partial or total loss of epidermal melanocytes. Many therapeutic modalities have been proposed for its treatment. In cases of stable vitiligo surgical treatments are preferred. Grafting by epidermal scraping is a variant of micrografts by punch technique. It is a new, simple and low cost technique, with high rates of repigmentation. The objective of this report is to demonstrate the authors' experience with this technique and discuss the results in an initial series of two cases (three lesions).

Recipient site preparation for epidermal graft in stable vitiligo by a special fraise

BACKGROUND: The only approach used in the refractory lesions of stable vitiligo is the surgical supply of melanocytes. Suction Blistering Epidermal Graft is one of the most common and effective techniques. There are multiple modalities, including the motor-driven diamond fraise, for the preparation of recipient sites in suction blistering epidermal graft with different repigmentation rates and complications. OBJECTIVES: To evaluate preparation of recipient site by a motor-driven dental lab finishing carbide bur. METHODS: Sixty-one stable, depigmented lesions were selected in 14 patients (9 women and 5men), aged 16-29 years, of which 9, 3 and 2 had localized, generalized and segmental vitiligo, respectively. Recipient site was prepared by a motor-driven dental lab finishing carbide bur. RESULT: Excellent repigmentation at the recipient site was observed in 53 out of 61 (86.9%) grafted lesions. Postinflammatory hyperpigmentation and perigraft halo were seen in 11 (18%) and 17 (27.9%) patients at the recipient site, respectively. CONCLUSION: Using a motor-driven dental lab finishing carbide bur to prepare the recipient site of suction blistering epidermal graft technique is reliable and effective, removing only the depigmented epidermis in a simple and safe manner, even on complex-shaped lesions and scar-prone sites. .

Inovação na correção estética dos retalhos cutâneos nasais / Innovation in aesthetic correction of nasal skin flaps

Os retalhos são muito utilizados na reconstrução cirúrgica da região nasal. Durante o pósoperatório desses pacientes habitualmente observa-se desnível da cicatriz em relação à pele vizinha, gerando insatisfação estética. Tal queixa frequentemente se mantém mesmo com a abrasão preconizada pela literatura entre 30 e 60 dias após o procedimento. Propõese associação de exereses tangenciais com lâmina de barbear (shavings) de toda a área do retalho até o aplainamento em relação à vizinhança, seguida de dermabrasão para refinamento de irregularidades ainda existentes. Os resultados são excelentes, tornando as cicatrizes menos perceptíveis, fato muito satisfatório em se tratando de área tão importante estética e socialmente

Flaps are widely used in the surgical reconstruction of the nasal region. During the post-operative period, an unattractive depressed scar in relation to the nearby skin can be observed. Even after abrasion ­ which the literature recommends 30-60 days after the procedure ­ this complaint often persists. This study proposes tangential excisions with a razor blade (shaving) of the flap's entire area until it is level with the surrounding region, followed by dermabrasion to refine any remaining irregularities. The results are deemed excellent since the scars become less noticeable ­ a very satisfactory outcome due to the aesthetic and social importance of the treated area.

Lifting de lábio superior associado à dermabrasão mecânica / Upper lip lifting associated with mechanical dermabrasion

O envelhecimento traz profundas modificações na face. Existe demanda crescente de procedimentos para tratamento estético facial. São exemplos as blefaroplatias, ritidoplastias, uso de substâncias preenchedoras e de toxina botulínica, entre outros. Infelizmente a região subnasal não recebe comparativamente igual atenção. Este relato tem por objetivo demonstrar o uso do lifting do lábio superior associado a dermabrasão como alternativa para se obter maior harmonia facial. Além disso, cabe reforçar a possibilidade de sua realização associada à de outros procedimentos cirúrgicos.

Efeito do uso de silicone em spray na reparação cutânea em procedimentos envolvendo ablação epidérmica: estudo de 20 casos / Use of silicone spray in cutaneous repair in dermal ablation procedures: study of 20 cases

Introdução: Não existe padronização dos cuidados imediatos após procedimentos de ablação epidérmica; a escolha cuidadosa dos produtos pode oferecer maior conforto ao paciente e menores riscos e períodos de recuperação e complicações Objetivo: Analisar a segurança e eficácia do silicone em spray na reparação tecidual de dois procedimentos de ablação epidérmica, o laser YSGG ablativo fracionado de 2790nm e a dermoabrasão. Métodos: Foram avaliados 20 pacientes com fotoenvelhecimento ou cicatrizes de acne, divididos aleatoriamente em dois grupos iguais; cada paciente usou de forma aleatorizada em cada hemiface, o silicone spray e vaselina líquida como controle. Todos os pacientes foram submetidos à medida de perda de água transepidérmica.Resultados: Todos os pacientes completaram o estudo; o eritema e a ardência foram os achados mais comuns, sendo significativamente mais frequentes na área de aplicação da vaselina. Conclusões: O silicone em spray se mostrou seguro e eficiente nos cuidados após procedimentos ablativos epidérmicos, sendo significativamente superior à vaselina no controle da ardência e do eritema.

Quimioabrasão para tratamento de rugas periorais: avaliação clínica e quantificação das células de langerhans epidérmicas / Chemabrasion for the treatment of perioral wrinkles: clinical analysis and epidermal Langerhans cells qualifi cation

Introdução: As técnicas ablativas oferecem bons resultados no rejuvenescimento da região perioral, mas acarretam alterações imunológicas locais. Objetivos: Avaliar a efi cácia e a segurança da quimioabrasão, no rejuvenescimento da região perioral, e quantifi car as células de Langerhans epidérmicas nos períodos pré e pós-operatório. Material e Métodos: Foi realizada a aplicação de ácido tricloroacético a 35%, seguida de dermabrasão manual com lixas d'água na região perioral, em 12 pacientes, sem antecedentes de infecção por herpes simples. O acompanhamento clínico e fotográfi co foi realizado 30 dias e 12 meses após a cirurgia. Técnicas de imuno-histoquímica quantifi caram as células de Langerhans antes e 30 dias após. Resultados: Todas as pacientes apresentaram melhora da pele da região perioral e redução da quantidade das células de Langerhans, após 30 dias (p = 0,002). Três desenvolveram herpes simples. Conclusões: A quimioabrasão oferece bons resultados para o tratamento de rugas periorais, e, após 30 dias, as células de Langerhans permanecem em menor número.

Introduction: Skin photoaging can be treated with ablative therapies that cause immunological changes to the skin. Objectives: To evaluate the results of chemabrasion in the treatment of perioral wrinkles and the change in number of Langerhans cells. Material and Methods: Application of 35% trichloroacetic acid followed by manual dermabrasion using waterproof sandpaper, in 12 female patients with perioral wrinkles and results were then examined 30 days and 1 year later. Immunohistochemistry was used to measure Langerhans' cells which were counted before treatment and 1 month post-treatment. Results: Improved perioral photoaging and reduced number of Langerhans' cells (p = 0.002) were seen in all patients. Conclusions: Chemabrasion provides effective results in the treatment of perioral wrinkles but Langerhans cells remain low for 30 days after therapy.