Preparation and Encapsulation Efficiency Determination of Diclofenac Sodium Liposomes / 中国药房

OBJECTIVE:To prepare diclofenac sodium liposomes and establish a method for their quality control.METHODS:The diclofenac sodium liposomes were prepared by pH gradient method;the particle shape and size were measured by transmission electrical miscroscope;the concentration and encapsulation efficiency of diclofenac sodium in the liposomes were determined by HPLC.RESULTS:The prepared lipisomes were uniformly distributed with particle size of 150～175 nm.The linear range of diclofenac sodium was 0.01～1 mg?mL-1(r=0.999 0,n=6)at an average recovery of 100.05%(RSD=1.21%,n=6).The encapsulation efficiency ranged from 52.4% to 85.8%.CONCLUSION:High encapsulation efficiency of diclofenac sodium liposomes can be obtained from the established method.