Production of lettuce cultivars in greenhouse in Humaitá, southern Amazonas state / Produção de cultivares de alface em casa de vegetação em Humaitá, sul do estado do Amazonas

Lettuce is the main leafy vegetable commercialized in Brazil and it is cultivated in many different environment conditions and regions. But, under high temperature and high light intensity lettuce plants tend to early flowering, making it difficult to obtain high-quality lettuce harvest. Thus, this study was aimed to evaluate the productive performance of lettuce cultivars in a greenhouse in the municipality of Humaitá, Amazonas state (AM). The cultivars used were Rafaela and Hanson, Veneranda, Mônica SF 31, Simpson, Solaris, Elba, Quatro Estações and Babá de Verão. The lettuce seedlings were transplanted 24 days after sowing into a greenhouse covered by 100 micra polyethylene transparent agrofilm. It was used bed with 33-m-long and 1-m-wide, using 30 cm x 30cm spacing between plants. The experimental design was randomized complete block with four replications. The parameters evaluated 32 days after transplanting were plant diameter, number of leaves, stem length and shoot dry mass. Cultivars Babá de Verão and Elba presented a better agronomic performance for cultivation in greenhouse in municipality of Humaitá, Amazonas state.

A alface é a principal folhosa comercializada no Brasil, sendo cultivada em muitas regiões e ambientais diferentes. Entretanto, sob altas temperaturas e elevada intensidade luminosa as plantas de alface tendem ao florescimento precoce, dificultando a obtenção de uma colheita de alface de alta qualidade. Assim, no presente estudo objetivou-se avaliar o desempenho produtivo de cultivares de alface em ambiente protegido no município de Humaitá, estado do Amazonas (AM). As cultivares usadas foram Rafaela e Hanson, Veneranda, Mônica SF 31, Simpson, Solaris, Elba, Quatro estações e Baba de Verão. As mudas de alface foram transplantadas 24 dias após a semeadura para casa de vegetação coberta com agrofilme de polietileno transparente de 100 micra. Foi utilizado canteiro com 33 m de comprimento e 1 m de largura, utilizando-se espaçamento de 30 cm x 30 cm entre plantas. O experimento foi conduzido utilizando-se delineamento em blocos completos casualizados com 4 repetições. Os parâmetros avaliados 32 dias após o transplantio foram o diâmetro da planta, número de folhas, comprimento do caule e massa seca da parte aérea. As cultivares Baba de Verão e Elba apresentaram melhor desempenho agronômico para o cultivo protegido no município de Humaitá, estado do Amazonas.

cDNA-AFLP analysis of gene expression differences between the flower bud and sprout-shoot apical meristem of Angelica sinensis (Oliv.) Diels.

Angelica sinensis (Oliv.) Diels (Umbelliferae) is a well-known medicinal plant mainly distributed in Gansu Province of China. Its local and global demand is significant because of its food and medicinal applications. However, the early bolting rate of Angelica sinensis (Oliv.) Diels reaches 20%-60%, which seriously affects its food and medicinal qualities. Thus, differences in gene expression between the flower bud and sprout-shoot apical meristem underwent analysis, by means of cDNA-amplified restriction fragment length polymorphism, to better understand the flowering mechanism. 64 primer sets, each of which amplified to 60 transcript-derived fragments (TDFs), were used. Among these TDFs, 26 were expressed specifically in the flower bud. After cloning and sequencing, 32 distinct sequences were obtained from these 26 TDFs, and 25 were found with homologous sequences in databases. Confirmation of differential expression of 13 sequences was obtained by semi-quantitative RT-PCR, their showing higher expression levels in flower buds. These homologous sequences encode transposable elements, pentatricopeptide repeat-containing proteins, DNA-binding transcription factors, zinc finger (B-box type) family proteins, NADP-dependent sorbitol 6-phosphate dehydrogenase (S6PDH), amongst others.

cDNA-AFLP analysis of gene expression differences between the flower bud and sprout-shoot apical meristem of Angelica sinensis (Oliv.) Diels

Angelica sinensis (Oliv.) Diels (Umbelliferae) is a well-known medicinal plant mainly distributed in Gansu Province of China. Its local and global demand is significant because of its food and medicinal applications. However, the early bolting rate of Angelica sinensis (Oliv.) Diels reaches 20 percent-60 percent, which seriously affects its food and medicinal qualities. Thus, differences in gene expression between the flower bud and sprout-shoot apical meristem underwent analysis, by means of cDNA-amplified restriction fragment length polymorphism, to better understand the flowering mechanism. 64 primer sets, each of which amplified to 60 transcript-derived fragments (TDFs), were used. Among these TDFs, 26 were expressed specifically in the flower bud. After cloning and sequencing, 32 distinct sequences were obtained from these 26 TDFs, and 25 were found with homologous sequences in databases. Confirmation of differential expression of 13 sequences was obtained by semi-quantitative RT-PCR, their showing higher expression levels in flower buds. These homologous sequences encode transposable elements, pentatricopeptide repeat-containing proteins, DNA-binding transcription factors, zinc finger (B-box type) family proteins, NADP-dependent sorbitol 6-phosphate dehydrogenase (S6PDH), amongst others.