Acquisition of 2C-like totipotency through defined maternal-effect factors.

Fully grown oocytes have the natural ability to transform 2 terminally differentiated gametes into a totipotent zygote representing the acquisition of totipotency. This process wholly depends on maternal-effect factors (MFs). MFs stored in the eggs are therefore likely to be able to induce cellular reprogramming to a totipotency state. Here we report the generation of totipotent-like stem cells from mESCs using 4MFs Hsf1, Zar1, Padi6, and Npm2, designated as MFiTLSCs. MFiTLSCs exhibited a unique and inherent capability to differentiate into embryonic and extraembryonic derivatives. Transcriptomic analysis revealed that MFiTLSCs are enriched with 2-cell-specific genes that appear to synergistically induce a transcriptional repressive state, in that parental genomes are remodeled to a poised transcriptional repression state while totipotency is established following fertilization. This method to derive MFiTLSCs could help advance the understanding of fate determinations of totipotent stem cells in a physiological context and establish a foundation for the development of oocyte biology-based reprogramming technology.

NMR Analysis of Extra Virgin Olive Oil of the Epirus Region of Greece with Emphasis on Selected Phenolic Compounds.

Extra virgin olive oil (EVOO) is recognized for its numerous health benefits, attributed to its rich phenolic components. NMR has emerged as a prevalent technique for precisely identifying these compounds. Among Mediterranean countries, Greece stands as the third-largest producer of olives, with the Epirus region notably advancing in olive cultivation, contributing significantly to the dynamic growth of the region. In this study, an NMR method was employed based on the acquisition of a 1H NMR spectrum along with multiple resonant suppression in order to increase the sensitivity. Using the above method, 198 samples of extra virgin olive oil, primarily sourced from the Epirus region, were analyzed, and both the qualitative and quantitative aspects of the phenolic compounds were obtained. In addition, we examined the effects of various factors such as variety, harvest month, and region origin on the phenolic compounds' concentration. The results revealed an average total phenolic content of 246 mg/kg, closely approaching the EU health claim limit of 250 mg/kg. Approximately 15% of the samples were confidently characterized as high-phenolic olive oil. The highest concentrations were observed in the Thesprotia samples, with several Lianolia varieties exceeding the total phenolic content of 400 mg/kg. Statistical tests demonstrated a significant influence of the olive variety and the month of fruit harvest on phenolic component concentration, followed by the region of origin. A very strong correlation was noted between the total phenolics content and the levels of oleocanthal and oleacein, with a correlation coefficient (r) of 0.924. Upon optimization of all factors affecting olive oil quality, the majority of the EVOOs from the Epirus region have the potential to be characterized as high in phenolic content.

Prevalence and Risk Factors of Enteral Feeding Intolerance in Critically Ill Patients and the Effectiveness of Preventive Treatments: A Prospective Study.

Background: Feeding intolerance (FI) is a prevalent cause of enteral nutrition (EN) disruption. Factors that can prevent FI are poorly described. Objectives: To determine the prevalence and risk factors associated with FI in critically ill patients and the effectiveness of preventive treatments. Patients and Methods: This prospective observational study included critically ill patients admitted to the ICU of a general hospital who received EN through a nasogastric or nasointestinal tube from March 2020 to October 2021. Independent sample t-test, repeated measurement analysis of variance, and multivariate analysis were used to explore independent risk factors and the efficacy of preventive treatments. Results: The study included 200 critically ill patients (mean age: 59.1 ± 17.8 years), of whom 131 were male. Most patients (58.50%) developed FI after a median EN duration of 2 days. The independent risk factors for FI were fasting for >3 days, high APACHE II score, and acute gastrointestinal injury (AGI) grade I before EN (P < 0.05). During EN, whole protein was found to be an independent preventive treatment that significantly decreased FI (P < 0.05), while before EN, early use of enema and gastric motility drugs in patients with abdominal distention/constipation significantly decreased FI (for both, P < 0.05). The preventive treatment group had significantly higher intake of the nutrient solution and significantly shorter invasive mechanical ventilation duration than the without preventive treatment group (for both, P < 0.05). Conclusion: In ICU patients receiving nasogastric or nasointestinal tube feeding, FI was frequent, occurred early, and was more frequent in patients with fasting >3 days, a high APACHE II score, and an AGI grade before EN. Preventive treatments can reduce FI prevalence and result in patients consuming more nutrient solutions and having shorter invasive mechanical ventilation duration. Chinese Clinical Trial Registry Registration no: ChiCTR-DOD-16008532.

Effects of Nitrogen Emissions on Fish Species Richness across the World's Freshwater Ecoregions.

The increasing application of synthetic fertilizer has tripled nitrogen (N) inputs over the 20th century. N enrichment decreases water quality and threatens aquatic species such as fish through eutrophication and toxicity. However, the impacts of N on freshwater ecosystems are typically neglected in life cycle assessment (LCA). Due to the variety of environmental conditions and species compositions, the response of species to N emissions differs among ecoregions, requiring a regionalized effect assessment. Our study tackled this issue by establishing regionalized species sensitivity distributions (SSDs) of freshwater fish against N concentrations for 367 ecoregions and 48 combinations of realms and major habitat types globally. Subsequently, effect factors (EFs) were derived for LCA to assess the effects of N on fish species richness at a 0.5 degree × 0.5 degree resolution. Results show good SSD fits for all of the ecoregions that contain sufficient data and similar patterns for average and marginal EFs. The SSDs highlight strong effects on species richness due to high N concentrations in the tropical zone and the vulnerability of cold regions. Our study revealed the regional differences in sensitivities of freshwater ecosystems against N content in great spatial detail and can be used to assess more precisely and comprehensively nutrient-induced impacts in LCA.

Bioaerosol-related studies in wastewater treatment plant with anaerobic-anoxic-oxic processes: Characterization, source analysis, control measures.

Sewage in wastewater treatment plants (WWTPs) can produce fugitive bioaerosols that pose a health risk to employees and residents. This study aimed to fugitive bioaerosols from two WWTPs with anaerobic-anoxic-oxic (AAO) processes, and bioaerosols control measures were proposed based on the results of these studies. It was found that the bioaerosols were mainly composed of microorganisms from dominant genera such as Romboutsia, Rubellimicrobium, Sphingomonas, Acidea, Cryptotrichosporon and water-soluble ions dominated by SO42-. Moreover, total suspended particulate (TSP), relative humidity (RH), wind speed (WS), Ca2+, NH4+, Na+, Cl-, NO3-, and K+ had positive effects on most dominant genera, while temperature (T) and SO42- had negative effects on most dominant genera. The source analysis showed that the bioaerosols in the indoor treatment facility's fine screen room and sludge dewatering plant mainly originated from sewage or sludge, and those in the aeration tank of the outdoor treatment facility mainly originated from the background air of WWTPs . By combining the characteristics of bioaerosols and the results of source analysis, targeted control measures were proposed from three aspects: source reduction of bioaerosol fugitives, control of bioaerosol propagation, and collection and treatment systems. This study provides the theoretical basis and ideas for controlling bioaerosols in WWTPs with AAO processes.

Factors that affect Pickering emulsions stabilized by mesoporous hollow silica microspheres.

HYPOTHESIS: Classical (solid particles stabilized) Pickering emulsions have been widely studied due to the irreversible adsorption of solid particles at the oil-water interface. Mesoporous hollow silica microspheres (MHSMs) are promising stabilizers for Pickering emulsion owing to its larger specific surface area and lower apparent density. However, this type of Pickering emulsion has not attracted enough attention. The stabilization mechanism of Pickering emulsion by MHSMs has not been studied in detail yet. EXPERIMENTS: Herein, stable Pickering emulsions were prepared using only MHSMs as stabilizers. In order to investigate its stabilization mechanism, the effect factors of size, shell thickness, wettability and concentration of MHSMs, and oil/water ratio on the stability of Pickering emulsions were analyzed deeply. FINDINGS: As a result, the stability of Pickering emulsion can be improved by MHSMs with smaller particle size and shell thickness. Also, MHSMs with the intermediate hydrophobicity and suitable oil/water ratio actually do favour for the stability of Pickering emulsion. As expected, the stability of Pickering emulsion can be enhanced by increasing the concentration of MHSMs in a certain range. The Pickering emulsions tend to achieve excellent stable state when the concentration of MHSMs is 1.25 mg/mL. All those results suggested that the stability of Pickering emulsions correlates directly to particle size, shell thickness, wettability and concentration of MHSMs, and oil/water ratio. This research paves a way for the fabrication of functional materials via Pickering emulsions.

Incidence of and risk factors for post-intensive care syndrome among Chinese respiratory intensive care unit patients: A cross-sectional, prospective study.

BACKGROUND: Patients transferred from the respiratory intensive care unit (ICU) can experience post-intensive care syndrome (PICS), which comprises cognitive, psychological, and physical disorders that seriously affect the quality of life. Therefore, it was necessary to explore the incidence of and the risk factors for PICS among respiratory ICU patients. OBJECTIVES: This study evaluated PICS among respiratory ICU patients and explored the risk factors for PICS. METHODS: This cross-sectional, prospective study was performed at one hospital in China. Using convenience sampling, 125 respiratory ICU patients from August 2018 to June 2019 were recruited for the study. The Mini-Mental State Examination, Confusion Assessment Method for the Intensive Care Unit, Hospital Anxiety and Depression Scale, Medical Research Council Scale, activities of daily living scale, Pittsburgh Sleep Quality Index, and the 14-item fatigue scale were used to comprehensively assess the patients' cognitive status, psychological status, and physiological status when entering the ICU and 2 weeks after leaving the ICU. Factors affecting PICS were measured using researcher-created questionnaires of patients' general information and disease-related information. RESULTS: Fifteen patients were lost to follow-up. Fifty-nine patients had PICS (incidence rate, 53.6%). Logistic regression showed that risk factors for PICS were age, invasive mechanical ventilation, noninvasive ventilator-assisted ventilation, and coronary heart disease (P < 0.05). CONCLUSION: The PICS incidence was high. Older age, longer invasive mechanical ventilation times, longer noninvasive ventilator times, and coronary heart disease were risk factors for PICS. ICU medical workers in China should pay more attention on PICS, know the risk factors, and implement preventive measures.

What affects the direct economic burden of non-communicable diseases on middle-aged and older adult people in Shaanxi Province?

Non-communicable diseases (NCDs) are the leading cause of death worldwide. NCDs affect the health status and the quality of life. In addition, continuous NCDs treatment expenses place a heavy economic burden on families and cause huge economic losses to the society. The prevention and treatment of NCDs and reduction of their economic burden are key public health issues. Considering middle-aged and older adult people as the focus, their basic socio-demographic characteristics and health behavior status of this group, and a pooled cross-sections regression model was then used to analyze the main factors affecting the direct economic burden. The results showed that from 2013 to 2018, the prevalence of NCDs among the middle-aged and older adult people in Shaanxi province as well as the direct economic burden of NCDs increased. The effect factors primarily included sex, age, employment status, income level, type of medical insurance, urban or rural residency, level of the health care-providing institutions, visiting times of 2-week, and length of hospital stay. Several measures can be taken to control the onset of NCDs and reduce their direct economic burden.

Chang impact analysis of level 3 COVID-19 alert on air pollution indicators using artificial neural network.

In this study, mean monthly and diurnal variations in fine particulate matters (PM2.5), nitrate, sulfate, and gaseous precursors were investigated during the Level 3 COVID-19 alert from May 19 to July 27 in 2021. For comparison, the historical data during the identical period in 2019 and 2020 were also provided to determine the effect of the Level 3 COVID-19 alert on aerosols and gaseous pollutants concentrations in Taichung City. A machine learning model using the artificial neural network technique coupled with a kinetic model was applied to predict NOx, O3, nitrate (NO3 -), and sulfate (SO4 2-) to investigate potential emission sources and chemical reaction mechanism. D during the Level 3 COVID-19 alert, a decrease in NOx concentration due to a decrease in traffic flow under the NOx-saturated regime was observed to enhance the secondary NO3 - and O3 formation. The present models were shown to predict 80.1, 77.0, 72.6, and 67.2% concentrations of NOx, O3, NO3 -, and SO4 2-, respectively, which could help decision-makers for pollutant emissions reduction policies development and air pollution control strategies. It is recommended that more long-term datasets, including water soluble inorganic salts (WIS), precursors including OH radicals, NH3, HNO3, and H2SO4, be provided by regulatory air quality monitoring stations to further improve the prediction model accuracy.

Human activities induce potential aquatic threats of micropollutants in Danjiangkou Reservoir, the largest artificial freshwater lake in Asia.

Freshwater biodiversity and ecosystem services may decline because of toxicant input, and other environmental variables often co-occur with contaminants to jeopardize the freshwater ecosystem. In this study, Danjiangkou Reservoir (DJKR) in central China was selected as the target research area to investigate the impact of multiple categories of micropollutants coupled with other stressors on the reservoir ecosystem. A total of 140 samples were collected from 28 sites in DJKR, and 124 micropollutants, including pesticides, organophosphate esters (OPEs), psychoactive substances, antiviral drugs, and pharmaceutical and personal care products, were quantified. A total of 108 micropollutants were detected in the water samples, with sum concentrations ranging from 82.35 ng·L-1 to 1436.57 ng·L-1, and 71 of them had a detection frequency above 50 %, indicating the prevailing micropollutant contamination in the reservoir. The most severe pollution and risks were observed in the tributaries of DJKR. Pesticides (neonicotinoid and triazine) and OPEs were the major contributors to the ecological risk in the reservoir. Insecticides, herbicides, and OPEs accounted for the majority of the risks to fish, algae, and invertebrates, respectively. The determined priority pollutants should be paid increased attention. Environmental variables and human activities, such as human land use, induced the potential aquatic threats of micropollutants in DJKR. Results demonstrated that micropollutant pollution was one of the dominant pressures faced by aquatic organisms and human beings, and human activities played important roles as well.

A state-of-the-art review on WWTP associated bioaerosols: Microbial diversity, potential emission stages, dispersion factors, and control strategies.

Wastewater treatment plants (WWTPs) associated bioaerosols have emerged as one of the critical sustainability indicators, ensuring health and well-being of societies and cities. In this context, this review summarizes the various wastewater treatment technologies which have been studied with a focus of bioaerosols emissions, potential emission stages, available sampling strategies, survival and dispersion factors, dominant microbial species in bioaerosols, and possible control approaches. Literature review revealed that most of the studies were devoted to sampling, enumerating and identifying cultivable microbial species of bioaerosols, as well as measuring their concentrations. However, the role of treatment technologies and their operational factors are investigated in limited studies only. Moreover, few studies have been reported to investigate the presence and concentrations of air borne virus and fungi in WWTP, as compared to bacterial species. The common environmental factors, affecting the survival and dispersion of bioaerosols, are observed as relative humidity, temperature, wind speed, and solar illumination. Further, research studies on recent episodes of COVID-19 (SARS-CoV-2 virus) pandemic also revealed that continuous and effective surveillance on WWTPs associated bioaerosols may led to early sign for future pandemics. The evaluation of reported data is bit complicated, due to the variation in sampling approaches, ambient conditions, and site activities of each study. Therefore, such studies need a standardized methodology and improved guidance to help informed future policies, contextual research, and support a robust health-based risk assessment process. Based on this review, an integrated sampling and analysis framework is suggested for future WWTPs to ensure their sustainability at social and/or health associated aspects.

Sensitivity analysis of heat dissipation factors in a hot oil pipeline based on orthogonal experiments.

The study of phase-change heat-transfer characteristics of crude oil has been one of the hot issues in the field of gathering and transportation. The process of phase-change heat transfer of crude oil involves many complicated problems such as natural convection treatment, latent heat treatment, phase-change interface determination and fluid characteristic change. A mathematical model based on the additional capacity heat method is proposed in this article, and the momentum equations of crude oil liquid phase are presented for Newtonian and non-Newtonian fluids. The aim of this study was to investigate the influence of different factors on the heat transfer performance during the shutdown process of an overhead pipe. Experiments were conducted to verify the model and the solution method; the experimental and model results showed good agreement with a maximum relative error of 4.57%. The temperature fields and solidification conditions of crude oil in pipelines under different shutdown conditions were determined, and the sensitivity of the main effect factors was determined through an orthogonal experiment. The results show that the order of influence was oil initial temperature >thickness of insulating layer >air temperature >thickness of wax layer. The results of the study have important guiding significance on the control of shutdown time and the determination of restarting schemes.

Effect factors related to a high probability of hemodialysis independence in newly diagnosed multiple myeloma patients requiring hemodialysis.

BACKGROUND: Renal failure is a severe complication of symptomatic myeloma, related to higher mortality. Recovery from dialysis dependence can lead to enormous survival benefits. We investigated the effect factors for probability of dialysis independence. METHODS: Retrospective data on 45 newly diagnosed MM (NDMM) patients with serious renal impairment and requiring hemodialysis were analyzed. The statistical methods including logistic regression analysis, Kaplan-Meier survival curves, the log-rank test and the Cox proportional hazards model for survival analysis were used in our study. RESULTS: Twenty-two of the 45 patients, who were on hemodialysis at diagnosis, became dialysis independence. In the logistic regression analysis, serum level of ß2-microglobulin, kidney disease history, involved free light chain, and achieving at least VGPR were significantly associated with reversibility from dialysis dependence. In addition, achieving hemodialysis discontinuation was related to better survival. The multivariate analyses demonstrated that reversibility from dialysis dependence, proteinuria < 3.5 g/24 h, and achieving at least VGPR were significantly associated with OS among NDMM patients requiring hemodialysis. CONCLUSION: Lower serum level of ß2-microglobulin and lower level of free light chain at diagnosis, achieving at least VGPR, and shorter kidney disease history are related to a high probability of dialysis independence in NDMM patients with serious renal failure requiring dialysis.

Analyses of effect factors associated with the postoperative dissatisfaction of patients undergoing open-door laminoplasty for cervical OPLL: a retrospective cohort study.

OBJECTIVES: This study aimed to investigate the effect factors associated with the postoperative dissatisfaction of patients undergoing open-door laminoplasty for cervical OPLL. METHODS: In this study, 194 patients, who underwent open-door laminoplasty for cervical OPLL from January 2009 to January 2016, were retrospectively reviewed. The Patient Satisfaction Index (PSI) was collected at discharge, 6 months, 1 year, and the last follow-up. According to the PSI, patients were divided into satisfied group and dissatisfied group. The possible effect factors included demographic variables and surgery-related variables. RESULTS: At discharge, 42 (21.6%) patients were in the dissatisfied group, as compared to the satisfied group, the hospitalization cost, hospital stay, postoperative depression, the axial neck pain, delayed wound healing, and VAS-neck had significant statistical differences. At 6-month follow-up, 25 (12.9%) patients were in the dissatisfied group. The axial neck pain and JOA score had significant statistical differences between the two groups, and no significant differences were found between the two groups in other items. At 1 year with 18 (9.3%) dissatisfied patients and last follow-up with 14 (7.2%) dissatisfied patients, the JOA score and symptom recurrence had significant statistical differences. For further analysis, the dissatisfied patients with axial neck pain at 6 months were significantly higher than that at other terms and the JOA score of the two groups increased gradually with prolonging of restoration years but compared with the dissatisfied group, the JOA scores were obviously better in the satisfied group at the last follow-up. CONCLUSIONS: Overall, to patients undergoing open-door laminoplasty for cervical OPLL, hospitalization cost and neck pain might be mainly associated with patient dissatisfaction at the early and middle recovery. Patient dissatisfaction at the long-term treatment outcome might be mainly associated with the low improvement rate of JOA score and symptom recurrence.

Investigating influencing factors of physical restraint use in China intensive care units: A prospective, cross-sectional, observational study.

OBJECTIVE: In this study, we characterised the use of physical restraints in three intensive care units (ICUs) in a general hospital in Nantong, China. Additionally, we explored risk factors potentially related to physical restraint use. BACKGROUND: Despite their numerous harmful effects, physical restraints are frequently used in ICUs worldwide. Few studies have investigated the factors that contribute to physical restraint use in Chinese hospitals. METHODS: We conducted a prospective, cross-sectional, observational study of 312 patients in three ICUs at a general hospital in China. The quantitative data were collected during a 5-month period using a physical restraint observation form and patient records. The data obtained were analysed using descriptive statistics. The independent risk factors for physical restraint use were assessed using a logistic regression model. RESULTS: Of the 312 patients in the three ICUs, 191 (61.2%) were restrained, and physical restraints were used more than once for 46 (24.1%) patients during their ICU stay. The median length of physical restrain use was 20 shifts (interquartile range = 10-36 shifts). Physical restraints were applied in 6664 of 12374 (53.9%) nurse shifts. The most common time at which physical restraints were applied was the beginning of the evening shift. According to the forward stepwise logistic regression analysis, delirium (P < 0.001), mechanical ventilation (P < 0.001), and age (P < 0.001) were independent risk factors for physical restraint use. The use of analgesics (P = 0.001) exerted an independent protective effect against physical restraint use. CONCLUSIONS: The overall prevalence of physical restraint use in Chinese ICUs was higher than that reported in previous investigations. The patients' nursing notes lacked complete physical restraint records, reflecting a need for standard guidelines and policies for physical restraint use in hospital ICUs in China. In addition, in this study, we explored the risk factors related to physical restraint use and found that age, delirium, mechanical ventilation, and analgesic use are associated with physical restraint use.

Prevalence and effect factors of dementia among the community elderly in Chongqing, China.

BACKGROUND: China is an ageing society, with around 150 million people aged 60 years or older. The prevalence of dementia will increase by more than 100% in the coming 20 years. This study aimed to investigate the prevalence and effects of dementia among the community elderly in Chongqing, China. METHODS: Cluster sampling was adopted. A sample of elderly individuals aged 60 years and older was extracted as research subjects from three counties within Chongqing, China. One-on-one interviews were conducted through a general information questionnaire, and the Mini-Mental State Examination, Instrumental Activity of Daily Living Scale, and Geriatric Depression Scale were administered. The investigated data were tested by χ2 , and the effect factors of dementia were analyzed by multiple logistic regression analysis. Statistical significance was set at P < 0.05. RESULTS: We distributed 1850 questionnaire, and the response rate was 100%. However, only 1781 questionnaires were able to be used in the study. Of the 1781 elderly respondents, 186 (10.44%) presented with dementia. Our findings revealed that differences in regions, age, marital status, education level, occupation, tobacco consumption, alcohol consumption, freshwater fish consumption, exercise, intensive labour, mah-jong- and chess-playing habits, media consumption (i.e. watching TV, listening to the radio, or reading the newspaper), body mass index, hypertension, coronary heart disease, and depression were statistically significant in the prevalence of dementia (P < 0.05). CONCLUSIONS: According to multiple logistic regression analyses, living in a rural area, older age, being single, obesity, hypertension, coronary heart disease, and depression are risk factors for dementia. In contrast, cessation of smoking, freshwater fish consumption, moderate or frequent exercise, intensive labour, daily housework, outdoor activities, media consumption, and social activities are protective factors against dementia among community elderly in Chongqing, China.

[Accuracy of computer-guided implant placement and influencing factors].

Digital technology is a new trend in implant dentistry and oral medical technology. Stereolithographic surgical guides, which are computer-guided implant placement, have been introduced gradually to the market. Surgeons are attracted to this approach because of it features visualized preoperative planning, simple surgical procedure, flapless implant, and immediate restoration. However, surgeons are concerned about the accuracy and complications of this approach. This review aims to introduce the classification of computer-guided implant placement. The advantages, disadvantages, and accuracy of this approach are also analyzed. Moreover, factors that may affect the outcomes of computer-guided implant placement are determined. Results will provide a reference to surgeons regarding the clinical application of this approach.

Accuracy of computer-guided implant placement and influencing factors / 华西口腔医学杂志

Digital technology is a new trend in implant dentistry and oral medical technology. Stereolithographic surgical guides, which are computer-guided implant placement, have been introduced gradually to the market. Surgeons are attracted to this approach because of it features visualized preoperative planning, simple surgical procedure, flapless implant, and immediate restoration. However, surgeons are concerned about the accuracy and complications of this approach. This review aims to introduce the classification of computer-guided implant placement. The advantages, disadvantages, and accuracy of this approach are also analyzed. Moreover, factors that may affect the outcomes of computer-guided implant placement are determined. Results will provide a reference to surgeons regarding the clinical application of this approach.

Effects of land use patterns on stream water quality: a case study of a small-scale watershed in the Three Gorges Reservoir Area, China.

In this study, we have considered the relationship between the spatial configuration of land use and water quality in the Three Gorges Reservoir Area. Using land use types, landscape metrics, and long-term water quality data, as well as statistical and spatial analysis, we determined that most water quality parameters were negatively correlated with non-wood forest and urban areas but were strongly positively correlated with the proportion of forest area. Landscape indices such as patch density, contagion, and the Shannon diversity index were able to predict some water quality indicators, but the mean shape index was not significantly related to the proportions of farmland and water in the study area. Regression relationships were stronger in spring and fall than in summer, and relationships with nitrogen were stronger than those of the other water quality parameters (R(2) > 0.80) in all three seasons. Redundancy analysis showed that declining stream water quality was closely associated with configurations of urban, agricultural, and forest areas and with landscape fragmentation (PD) caused by urbanization and agricultural activities. Thus, a rational land use plan of adjusting the land use type, controlling landscape fragmentation, and increasing the proportion of forest area would help to achieve a healthier river ecosystem in the Three Gorges Reservoir Area (TGRA).

Evaluation of preventive medicine studnent's quality of life and its relationship to training mode in school / 中华医学教育探索杂志

Objective To understand the situation of undergraduates' quality of life and analyze the influence factors in order to offer basis for improving personnel training mode of public health department in Wuhan University. Methods Questionnaire survey has been conducted among all undergraduates of public health department in Wuhan University. The questionnaire includes the level of internationalization, compound and practice innovation activities. At the same time, students' quality of life has been investigated using the WHOQOL-BREF. SPSS13.0 has been used to make statistical analysis. Scores between groups has been compared utilizing t test, analysis of variance has been made and influence factors has been ana-lyzed utilizing multiple linear regression. Results The average of the undergraduate graduates' quality of life total score is(3.54±0.77),with a scoring rate of 70.9%. The dimension with the highest scoring rate is social relations, and its scoring rate is 75.1%. The dimension with the lowest scoring rate is environment, with scoring rate 65.9%. Compared with the norm, the scores of the overall quality of life, physical health, social relations and environment are all above the norm(P<0.05). The result of multiple linear regression indicates that female(t=-2.338, P=0.020), students from high income family(t=3.512, P=0.001) and students with a high degree of practice innovation activity participation(t=3.515, P=0.001) have a high quality of life. Conclusion The students' quality of life from school of public health, Wuhan University is slightly higher than the general population. The quality of life is influenced significantly by part of cultivating ways. There is a need which personnel training mode is innovated in order to improve the quality of cultivation.