¿Es necesaria la formación anual sobre reanimación cardiopulmonar en el colegio? / Is annual training on cardiopulmonary resuscitation necessary at school?

Objetivo: evaluar los conocimientos sobre reanimación cardiopulmonar (RCP) adquiridos en estudiantes de 5º de primaria de un colegio de Zaragoza (España) tras realizar un taller teórico-práctico y las diferencias en función de su repetición o no el año posterior.Método: ensayo controlado aleatorizado realizado entre 2019 y 2021. Se incluyó a 72 estudiantes matriculados que se asignaron aleatoriamente a dos grupos iguales. Un grupo recibió un taller teórico-práctico sobre RCP en dos sesiones de una hora, otro repitió la formación también el curso siguiente. Se midieron edad, sexo y conocimientos mediante test autoadministrado diseñado ad hoc (puntuación 0 a 13 máx.) antes y después del primer año y tras el segundo. Se realizaron índices de estadística descriptiva y contraste de hipótesis.Resultados: participaron 72 alumnos de 10,6 años de media. La puntuación se incrementó de manera estadísticamente significativa en ambos grupos (valor p< 0,001) el primer año. En el segundo año, la mediana de la puntuación total obtenida fue 11,0 (RIC= 2,0) en el grupo sin repetición y 13,0 (RIC= 1,0) en el que sí se repitió (p< 0,001). Este último grupo identificó mejor la comprobación inicial de consciencia, la actuación mientras llega ayuda, la actuación en inconsciencia sin respiración y la relación compresiones/ventilaciones (p< 0,05).Conclusión: la participación en el taller mejoró el conocimiento de los estudiantes. Quienes recibieron de nuevo la formación el año siguiente obtuvieron mayores conocimientos que aquellos que solo lo obtuvieron en una ocasión.(AU)

Objective: to evaluate the knowledge about cardiopulmonary resuscitation acquired by students in their 5th year of Primary School from a school in Zaragoza (Spain) after conducting a theoretical-practical workshop, and the differences based on whether it was repeated or not on the following year.Method: a controlled randomized study conducted between 2019 and 2021, which included 72 enrolled students who were randomly assigned to two equal arms. One of the arms attended a theoretical-practical workshop on cardiopulmonary resuscitation (CPR) in two one-hour sessions; the other arm also repeated the training the following year. Age, gender and knowledge were measured through a self-administered test which was designed ad hoc (scores from 0 to 13 maximum) before and after the first year and after the second year. Descriptive statistics indexes and hypothesis contrast were used.Results: the study included 72 students with 10.6 years as mean age. There was a statistically significant increase in scores in both arms (p value< 0.001) on the first year. On the second year, the median total score obtained was 11.0 (IQR= 2.0) in the arm without repetition and 13.0 (IQR= 1.0) in the arm with repetition (p< 0.001). This last arm presented better identification of the initial verification of consciousness, the action while waiting for help, the action in lack of consciousness without respiration, and the compression / ventilation ratio (p< 0.05).Conclusion: participation in the workshop improved the knowledge of students. Those who received training again on the following year obtained higher knowledge than those who only received it once.(AU)

Comparing treatment strategies to reduce antibiotic resistance in an in vitro epidemiological setting.

The rapid rise of antibiotic resistance, combined with the increasing cost and difficulties to develop new antibiotics, calls for treatment strategies that enable more sustainable antibiotic use. The development of such strategies, however, is impeded by the lack of suitable experimental approaches that allow testing their effects under realistic epidemiological conditions. Here, we present an approach to compare the effect of alternative multidrug treatment strategies in vitro using a robotic liquid-handling platform. We use this framework to study resistance evolution and spread implementing epidemiological population dynamics for treatment, transmission, and patient admission and discharge, as may be observed in hospitals. We perform massively parallel experimental evolution over up to 40 d and complement this with a computational model to infer the underlying population-dynamical parameters. We find that in our study, combination therapy outperforms monotherapies, as well as cycling and mixing, in minimizing resistance evolution and maximizing uninfecteds, as long as there is no influx of double resistance into the focal treated community.

Fundamental Frequency in Excised Human Larynges after Anterior Glottic Web Formation of Various Extents.

OBJECTIVE: To determine the effect of vocal fold anterior web formation on fundamental frequency with a cadaveric excised larynx model. STUDY DESIGN: Experimental study with excised human larynges. SETTING: Academic tertiary care hospital. SUBJECTS AND METHODS: Sixteen freshly excised human larynges were evaluated with high-speed videoendoscopy and digital kymography during artificially produced vibration. Each larynx was assessed in 4 conditions: preoperative controls and after 25%, 33%, and 50% decreases in the vibratory portion of the vocal folds. The following parameters were evaluated: fundamental frequency, periodicity, vocal fold vibration amplitude, phase symmetry, and glottic closure. RESULTS: The mean fundamental frequencies were 208.87, 250.20, 292.37, and 342.67 Hz for preoperative controls and 25%, 33%, and 50% reductions in vibratory length of the vocal folds, respectively. Fundamental frequency increased with each increase in anterior glottic web extent, and the difference among the groups was statistically significant in absolute values in hertz and in semitone elevation. The mathematical models for estimating postoperative fundamental frequency had a statistically significant coefficient. The vibration of the vocal folds remained periodic in all larynges before and after the procedures. CONCLUSION: There was a significant and progressive increase in the fundamental frequency with each enlargement of anterior glottic web. Based on the control frequency, mathematical models could estimate the value of the fundamental frequency after the procedure.

Experimental Epidemiology of Antibiotic Resistance: Looking for an Appropriate Animal Model System.

Antibiotic resistance is recognized as one of the major challenges in public health. The global spread of antibiotic resistance is the consequence of a constant flow of information across multi-hierarchical interactions, involving cellular (clones), subcellular (resistance genes located in plasmids, transposons, and integrons), and supracellular (clonal complexes, genetic exchange communities, and microbiotic ensembles) levels. In order to study such multilevel complexity, we propose to establish an experimental epidemiology model for the transmission of antibiotic resistance with the cockroach Blatella germanica. This paper reports the results of five types of preliminary experiments with B. germanica populations that allow us to conclude that this animal is an appropriate model for experimental epidemiology: (i) the composition, transmission, and acquisition of gut microbiota and endosymbionts; (ii) the effect of different diets on gut microbiota; (iii) the effect of antibiotics on host fitness; (iv) the evaluation of the presence of antibiotic resistance genes in natural- and lab-reared populations; and (v) the preparation of plasmids harboring specific antibiotic resistance genes. The basic idea is to have populations with higher and lower antibiotic exposure, simulating the hospital and the community, respectively, and with a certain migration rate of insects between populations. In parallel, we present a computational model based on P-membrane computing that will mimic the experimental system of antibiotic resistance transmission. The proposal serves as a proof of concept for the development of more-complex population dynamics of antibiotic resistance transmission that are of interest in public health, which can help us evaluate procedures and design appropriate interventions in epidemiology.

Cicatrização de úlceras teciduais não infectadas tratadas com papaína / Tecidual ulcers cicatrization no infected by papaína

estudar os aspectos histológicos da cicatrização de lesões ulcerativas assépticas tratadas com soluções de papaína. MÉTODO: estudo experimental, utilizandos ratos Wistar (n=18), machos, adultos, que após procedimento cirúrgico, para a retirada de uma seção quadrada de pele da região cervical, foram distribuídos em dois grupos: Grupo I - Controle (n=9), sem tratamento; Grupo II ? Tratados (n=9), com tratamento com soluções de papaína de 10%, 6% e 4%, de acordo com as características morfológicas macroscópicas das lesões. A análise histológica das áreas lesadas, coradas com Hematoxilina-eosina e tricrômico de Masson, foi realizada com 7, 14 e 21 dias. RESULTADOS: a papaína auxiliou na modulação do processo inflamatório; formação e amadurecimento do tecido de granulação, e organização das fibras colágenas; acelerando a proliferação e organização da epiderme nas lesões ulcerativas em todos os dias estudados. CONCLUSÕES: estes dados reforçam e complementam pesquisas que relacionam os efeitos cicatrizantes da papaína sobre lesões ulcerativas principalmente por facilitar a organização do tecido de granulação e possivelmente por modular a resposta inflamatória

OBJECTIVES: study the histology of healing of ulcerative wound treated with papaine solutions METHODS: a square section of skin was surgically removed from the cervical region of adult-male Wistar rats (n=18), which were divided into two groups: Group I - Control (n=9), no treatment; and Group II ? Treated (n=9) with treatment with papaine solutions of 10, 6 and 4%, according to the macroscopic morphological characteristics of the lesions. A histological analysis was made of the lesions, stained with hematoxilin and eosin and with Masson?s trichrome, at 7, 14 and 21 days. RESULTS: the papaine aided in the modulation of the inflammatory process, in the formation and maturation of the granulation tissue and in the organization of the collagenous fibers; accelerating the proliferation and organization of the epidermis at the ulcerative lesions throughout

Efeito do ácido zoledrônico em tíbias de ratas ooforectomizadas: estudo prospectivo e randomizado / Effects of zoledronic acid on ooforectomized rats' tibiae: a prospective and randomized study

OBJETIVO: Investigar as repercussões clínicas, biomecânicas e histomorfométricas do ácido zoledrônico em tíbias de ratas osteoporóticas, após ooforectomia bilateral. MÉTODOS: Foram estudadas, prospectivamente, 40 ratas da linhagem Wistar (Rattus novergicus albinus). Com 60 dias de vida, os animais foram aleatorizados em dois grupos de acordo com o procedimento cirúrgico: ooforectomia bilateral (O) (n=20) e pseudo-cirurgia ("sham") (P) (n=20). Após 30 dias, os animais foram divididos em quatro subgrupos, de acordo com a administração de 0,1mg/kg de ácido zoledrônico (AZ) ou água destilada (AD): OAZ (n=10), OAD (n=10), PAZ (n=10) e PAD (n=10). Após 12 meses, os animais foram eutanasiados e suas tíbias analisadas. No estudo clínico foi considerado o peso dos animais; no estudo biomecânico foram realizados ensaios compressivos e na análise histomorfométrica foi determinada a área trabecular óssea. RESULTADOS: Os grupos "O" tiveram aumento de peso significativamente maior que os grupos "P" (p=0,005). Os grupos OAZ e PAZ tiveram aumento, não significativo, de peso quando comparados aos grupos OAD (p=0,47) e PAD (p=0,68). Os grupos com ácido zoledrônico e com água destilada suportaram carga máxima, semelhante (p=0,2), no momento em que ocorreu fratura. Nos grupos com ácido zoledrônico verificou-se o aumento não significante da área trabecular óssea quando comparados aos grupos com água destilada (p=0,21). Houve correlação positiva entre a área trabecular e a carga máxima (p=0,04; r=0,95). CONCLUSÃO: O ácido zoledrônico não influiu significativamente no peso dos animais. Os resultados mostraram aumento, não significante, tanto da resistência óssea diafisária tibial, como da área trabecular óssea.

OBJECTIVE: To investigate clinical, biomechanic and histomorphometric effects of zoledronic acid on osteoporotic rats'tibiae after bilateral ooforectomy. METHODS: 40 female Wistar (Rattus novergicus albinus) rats were prospectively studied. On the 60th day of life, the animals were randomized into two groups according to the surgical procedure: bilateral ooforectomy (O) (n=20) and sham surgery ("sham") (P) (n=20). After 30 days, the animals were divided into four groups, according to the administration of zoledronic acid (ZA) 0.1mg/kg or distilled water (DW): OZA (n=10), ODW (n=10), PZA (n=10) and PDW (n=10). After 12 months, the animals were sacrificed, and had their tibiae assessed. In the clinical study, animals'weight was considered; in the biomechanical study, compressive assays were applied and, in the histomorphometric analysis, the bone trabecular area was determined. RESULTS: "O" groups showed a significantly greater weight gain than "P" groups (p=0.005). Groups OZA and PZA showed an insignificant weight gain when compared to ODW (p=0.47) and PDW (p=0.68). The groups receiving zoledronic acid and distilled water were able to bear maximum load, similar (p=0.2), at the moment of fracture. In the groups receiving zoledronic acid, an insignificant increase of the bone trabecular area was found when compared to the groups receiving distilled water (p=0.21). There was a positive correlation between trabecular area and maximum load (p=0.04; r=0.95). CONCLUSION: Zoledronic acid did not significantly influence animals' weight. The results showed an insignificant increase both of the tibial shaft bone resistance and the bone trabecular area.

EFFECTS OF ZOLEDRONIC ACID ON OOFORECTOMIZED RATS' TIBIAE: A PROSPECTIVE AND RANDOMIZED STUDY.

UNLABELLED: To investigate clinical, biomechanic and histomorphometric effects of zoledronic acid on osteoporotic rats' tibiae after bilateral ooforectomy. METHODS: 40 female Wistar (Rattus novergicus albinus) rats were prospectively studied. On the 60th day of life, the animals were randomized into two groups according to the surgical procedure: bilateral ooforectomy (O) (n=20) and sham surgery ("sham") (P) (n=20). After 30 days, the animals were divided into four groups, according to the administration of zoledronic acid (ZA) 0.1mg/kg or distilled water (DW): OZA (n=10), ODW (n=10), PZA (n=10) and PDW (n=10). After 12 months, the animals were sacrificed, and had their tibiae assessed. In the clinical study, animals' weight was considered; in the biomechanical study, compressive assays were applied and, in the histomorphometric analysis, the bone trabecular area was determined. RESULTS: "O" groups showed a significantly greater weight gain than "P" groups (p=0.005). Groups OZA and PZA showed an insignificant weight gain when compared to ODW (p=0.47) and PDW (p=0.68). The groups receiving zoledronic acid and distilled water were able to bear maximum load, similar (p=0.2), at the moment of fracture. In the groups receiving zoledronic acid, an insignificant increase of the bone trabecular area was found when compared to the groups receiving distilled water (p=0.21). There was a positive correlation between trabecular area and maximum load (p=0.04; r=0.95). CONCLUSION: Zoledronic acid did not significantly influence animals' weight. The results showed an insignificant increase both of the tibial shaft bone resistance and the bone trabecular area.

The Effect of Parasites on Host Population Density and Extinction: Experimental Epidemiology with Daphnia and Six Microparasites.

Parasites have been shown to reduce host density and to induce host population extinction in some cases but not in others. Epidemiological models suggest that variable effects of parasites on individual hosts can explain this variability on the population level. Here, we aim to support this hypothesis with a specific epidemiological model using a cross-parasite species approach. We compared the effect of six parasites on host fecundity and survival to their effects on density and risk of extinction of clonal host populations. We contrast our empirical results of population density with predictions from a deterministic model and contrast our empirical results of host and parasite extinction rates with those predicted by a stochastic model. Five horizontally transmitted microparasites (two bacteria: white bacterial disease, Pasteuria ramosa; two microsporidia: Glugoides intestinalis, Ordospora colligata; one fungus: Metschnikowiella biscuspidata); and six strains of a vertically transmitted microsporidium (Flabelliforma magnivora) of the planktonic crustacean Daphnia magna were used. In life table experiments, we quantified fecundity and survival in individual parasitized and healthy hosts and compared these with the effect of the parasites on host population density and on the likelihood of host population extinction in microcosm populations. Parasite species varied strongly in their effects on host fecundity, host survival, host density reduction, and the frequency with which they drove host populations to extinction. The fewer offspring an infected host produced, the lower the density of an infected host population. This effect on host density was relatively stronger for the vertically transmitted parasite strains than for the horizontally transmitted parasites. As predicted by the stochastic simulations, strong effects of a parasite on individual host survival and fecundity increased the risk of host population extinction. The same was true for parasite extinctions. Our results have implications for the use of microparasites in biological control programs and for the role parasites play in driving small populations to extinction.