Assessing the conservation value of cemeteries to urban biota worldwide.

Cemeteries are key urban green spaces with multifaceted societal and ecological importance. Their biodiversity is shaped by unique environmental and cultural factors. They can potentially protect rare and endangered species, yet their conservation value compared with other urban green spaces remains largely unexplored. We sought to fill this gap by systematically reviewing literature to investigate the conservation value of cemeteries relative to other urban green spaces (botanical gardens, institutional premises, natural remnants, and parks) by comparing species richness and proportions of native and unique species. We analyzed data from 70 papers covering 50 cities in 27 countries with linear and binomial mixed-effects models at both site and city level. Cemetery conservation value was similar to urban parks, except for the proportion of unique species, for which parks had significantly higher proportions (21.9% vs. 14.2%, p < 0.001). Cemeteries hosted slightly higher proportions of native species at the city level than botanical gardens (99.7% vs. 99.6%, p < 0.001) and institutional green spaces (96.3% vs. 94.1%, p = 0.034) and proportions comparable to parks and natural remnants (p > 0.05). They also had similar or higher values than institutional premises in species richness and unique species proportions (p > 0.05) and a higher site-level proportion of native species (p < 0.001). In contrast, species richness (slopes = -0.11 and -0.25, respectively) and unique species proportions (4.4% and 6.9%, respectively, p < 0.001 for both) were lower in cemeteries than in remnants of natural areas and in botanical gardens. The conservation value of cemeteries and parks was similar for animals, but parks had a higher value for plants. Overall, cemeteries were generally at least as valuable as some other green spaces for urban biodiversity and mostly native biota. Their religious and cultural significance suggests they will remain intact in the long term; thus, it is essential to prioritize and further promote their biodiversity in conservation and sustainable urban design plans.

Análisis del valor de conservación que tienen los cementerios para la biota urbana a nivel mundial Resumen Los cementerios son espacios urbanos verdes con una importancia social y ecológica multifacética. Su biodiversidad está moldeada por factores ambientales y culturales únicos. Los cementerios tienen el potencial para proteger especies raras y en peligro, pero su valor de conservación sigue sin ser explorado cuando se compara con otros espacios urbanos verdes. Buscamos llenar este vacío con una revisión sistemática de la bibliografía para investigar el valor de conservación de los cementerios en relación a otros espacios urbanos verdes (jardines botánicos, sedes institucionales, remanentes naturales y parques) al comparar la riqueza de especies y la proporción de especies nativas y únicas. Analizamos con modelos lineales y binomiales de efectos mixtos a nivel de sitio y de ciudad los datos de 70 artículos enfocados en 50 ciudades de 27 países. El valor de conservación de los cementerios fue similar al de los parques urbanos, excepto para la proporción de especies únicas, para la cual los parques tuvieron una proporción significativamente más alta (21.9% vs. 14.2%, p<0.001). A nivel de ciudad, los cementerios albergaron una proporción ligeramente mayor de especies nativas en comparación con los jardines botánicos (99.7% vs. 99.6%, p<0.001) y las sedes institucionales (96.3% vs. 94.1%, p = 0.034) y tuvieron una proporción comparable a los parques y los remanentes naturales (p>0.05). Los cementerios también tuvieron un valor similar o mayor a los espacios verdes institucionales en cuanto a riqueza de especies y proporción de especies únicas (p>0.05) y una proporción mayor a nivel de sitio de especies nativas (p<0.001). Al contrario, la riqueza de especies (pendientes = ­0.11 y ­0.25, respectivamente) y la proporción de especies únicas (4.4% y 6.9%, respectivamente, p<0.001 para ambas) fueron menores en los cementerios que en los remanentes de las áreas naturales y en los jardines botánicos. El valor de conservación de los cementerios y los parques fue similar para los animales, pero los parques tuvieron un valor mayor para las plantas. En general, los cementerios fueron al menos tan valiosos como otros espacios verdes para la biodiversidad urbana y casi toda la biota nativa. El significado religioso y cultural que tienen los cementerios sugiere que seguirán intactos a largo plazo; por ello, es esencial priorizar y promover aún más su biodiversidad en la conservación y en los planes de diseño urbano sustentable.

A first revision of the Andrena of Iraq (Hymenoptera, Andrenidae), with the description of two new species from Iraqi Kurdistan and additional records from surrounding countries.

Iraq is a large country in the Middle East region that borders both Turkey and Iran, countries known to host two of the largest bee faunas globally, as expected for a group of insects that favour dry to Mediterranean climates. Despite this huge regional species richness, the bee fauna of Iraq is chronically understudied and poorly known, both in relative and absolute terms. This is true for the hyper-speciose bee genus Andrena, for which only 17 species have been previously published for Iraq. This work is the first modern contribution to the revision of the Andrena fauna of Iraq. Based on new specimen collections in Duhok Governorate (Iraqi Kurdistan) during 2023, a revised total of 59 Andrena species for Iraq (42 species recorded for the first time) is presented, including the description of two new species: Andrena (Aciandrena) duhokensis Wood, sp. nov. and Andrena (Notandrena) baiocchii Wood, sp. nov. The unknown males of A. (Micrandrena) elam Wood, 2022, A. (Micrandrena) obsidiana Wood, 2022, and A. (Notandrena) ayna Wood, 2023 are described. Andrenabakrajoensis Amin & Mawlood, 2019, syn. nov. is synonymised with A. (Holandrena) variabilis Smith, 1853. Additional records are presented from nearby Middle Eastern countries, particularly Lebanon. These results highlight the fundamentally understudied nature of the Iraqi Andrena fauna.

A new species of holothuroid from the Labrador Sea (eastern Canada): Pseudothyonelabradorensis sp. nov. (Echinodermata, Holothuroidea, Dendrochirotida, Sclerodactylidae).

A new species of holothuroid, Pseudothyonelabradorensis sp. nov. (order Dendrochirotida and family Sclerodactylidae), was discovered off the coast of Labrador (eastern Canada) at a depth of 740-969 m. Two specimens were described based on morphological and genetic parameters. Distinctive characters included pinkish body colour, presence of tube feet on a 'tail', supporting rod-shaped ossicles in the tube feet, and rod-shaped ossicles in the tentacles. To investigate its phylogenetic relationships, partial sequences of COI were obtained for the new species as well as for the type species P.raphanus and another North Atlantic species P.serrifera. According to the phylogenetic analysis, P.labradorensis sp. nov. appeared in a well-supported clade with P.raphanus and P.serrifera. Molecular data also suggest polyphyly of the genus, showing the Northeast Pacific species Pseudothyonebelli recovered outside of the clade containing the type species. Pseudothyonelabradorensis sp. nov. is the first species of the genus from the Northwest Atlantic. A key to the North Atlantic Pseudothyone is provided.

Incorporation of human-wildlife interactions in ecosystem-based management to enhance conservation of endangered guitarfish.

Growing human use of the marine environment increases the proximity of humans to marine wildlife and thus likely increases human-wildlife interactions. Such interactions influence perceptions of nature and promote or undermine conservation. Despite their importance, human-wildlife interactions are rarely considered in ecosystem-based marine spatial planning (MSP). Ideally, these interactions should be identified and considered in ecosystem-based management (EBM), which is often purported to be the basis for MSP. We used Marxan software and data from a citizen science project documenting location, species, age, sex, and activity type to identify regions along Israel's coast with a high probability of encounters between people and 2 species of guitarfish. We considered the geographic distribution of these encounters and the various activities undertaken by the reporting observers. We ran 4 scenarios in Marxan. Two had conservation goals of 30% and 50% guitarfish habitat protection. In the third and fourth scenarios, we added a 50% conservation goal of human leisure activities to each guitarfish conservation goal. We also conducted a gap analysis between our guitarfish conservation goals and the Israel Nature and Parks Authority's master plan for marine protected areas. We found the park authority was close to meeting the 30% goal but was far from meeting the conservation goal of 50% of guitarfish habitat conservation. Different human uses were more likely to interact with different life stages of guitarfish, and different recreational activities occurred in different areas. Identifying areas of specific human use showed which activities should be addressed in conservation management decisions. Our addition of certain recreational uses to the model of habitat conservation showed how enhancing human dimensions in conservation planning can lead to more holistic ecosystem-based conservation necessary for effective marine planning.

Incorporación de las interacciones humano­fauna dentro de la gestión basada en el ecosistema para mejorar la conservación del pez guitarra en peligro Resumen El uso creciente que el humano le da al mar incrementa la cercanía de las personas con la fauna marina, lo que probablemente incrementa las interacciones humano­fauna. Dichas interacciones influyen sobre las percepciones que se tienen de la naturaleza y promueven debilitan la conservación. A pesar de su importancia, pocas veces se consideran las relaciones humano­fauna dentro de la planeación espacial basada en ecosistemas marinos (PEM). Lo ideal debería ser la identificación y consideración de estas interacciones dentro de la gestión basada en el ecosistema (GBE), la cual con frecuencia se considera como la base de la PEM. Usamos software Marxan y datos de un proyecto de ciencia ciudadana que documenta la ubicación, especie, edad, sexo y tipo de actividad para identificar las regiones de la costa de Israel con una alta probabilidad de encuentros entre las personas y dos especies de pez guitarra. Consideramos la distribución geográfica de estos encuentros y las diferentes actividades que realizan los observadores. En Marxan corrimos cuatro escenarios. Dos de los escenarios contaban objetivos de conservación del 30% y 50% de la protección del hábitat del pez guitarra. En los otros dos escenarios, añadimos un objetivo de conservación de 50% de las actividades humanas de recreación a los objetivos uno y dos. También realizamos un análisis de brecha entre los objetivos de conservación del pez guitarra y el plan maestro para las áreas marinas protegidas de la Autoridad de Parques y Naturaleza de Israel. Descubrimos que esta autoridad estaba cerca de lograr el objetivo del 30% pero lejos del de 50% de la conservación del hábitat del pez guitarra. Fue más probable que los diferentes usos humanos interactuaran con diferentes estadios de vida del pez guitarra y las diversas actividades recreativas ocurrieron en áreas distintas. La identificación de las áreas con un uso humano específico mostró cuáles actividades deberían abordarse en las decisiones de gestión de la conservación. La suma de ciertos usos recreativos al modelo de conservación del hábitat mostró cómo aumentar las dimensiones humanas en la planeación de la conservación puede derivar en una conservación basada en el ecosistema más holística, necesaria para la planeación marina eficiente.

Genome assembly catalog for species in the Japanese Red List: unlocking endangered biodiversity through genomic inventory.

Improvements in DNA sequencing technology are allowing the dramatic increase of whole genome data for a wide variety of species. Such genome sequence data can assist the monitoring of intraspecific genetic diversity, but is often lacking for threatened species. In this project, we focused on the national Red List, a catalog of extinct and threatened species, issued by the Japanese government. We combined the data included in it with the record of genome assembly in NCBI and tabulated the assembly availability of the species in the list. The combined data shows a low percentage (2.1%) of the availability of whole genome sequence data for the taxa ranked on the Japanese Red List as well as a strong bias towards mammals and birds in Animalia and vascular plants in Plantae. Our data presentation highlights potential systematic limitations in genome sequencing (e.g., budget for sequencing large genomes of amphibians) and instructs future policies including which taxon needs more effort for genome sequencing. The resultant tables are available in the original website https://treethinkers.nig.ac.jp/redlist/ and are regularly updated.

Biochar alters the soil fauna functional traits and community diversity: A quantitative and cascading perspective.

With the widespread use of biochar, the cascading effects of biochar exposure on soil fauna urgently require deeper understanding. A meta-analysis quantified hierarchical changes in functional traits and community diversity of soil fauna under biochar exposure. Antioxidant enzymes (24.1 %) did not fully mitigate the impact of MDA (13.5 %), leading to excessive DNA damage in soil fauna (21.2 %). Concurrently, reproduction, growth, and survival rates decreased by 20.2 %, 8.5 %, and 21.2 %, respectively. Due to a 39.7 % increase in avoidance behavior of soil fauna towards biochar, species richness ultimately increased by 80.2 %. Compared to other feeding habits, biochar posed a greater threat to the survival of herbivores. Additionally, macrofauna were the most sensitive to biochar. The response of soil fauna also depended on the type, size, concentration, and duration of biochar exposure. It should be emphasized that as exposure concentration increased, the damage to soil fauna became more severe. Furthermore, the smaller the biochar sizes, the greater the damage to soil fauna. To mitigate the adverse effects on soil fauna, this study recommens applying biochar at appropriate times and selecting large sizes in low to medium concentrations. These findings confirm the threat of biochar to soil health from the perspective of soil fauna.

A dataset of ground-dwelling nocturnal fauna for object detection and classification.

The exploration of ground-dwelling nocturnal fauna represents a significant challenge due to its broad implications across various sectors, including pesticide management, crop yield forecasting, and plant disease identification. This paper unveils an annotated dataset, BioAuxdataset, aimed at facilitating the recognition of such fauna through field images gathered across multiple years. Culled from a collection exceeding 100,000 raw field images over a span of four years, this meticulously curated dataset features seven prevalent species of nocturnal ground-dwelling fauna: carabid, mouse, opilion, slug, shrew, small-slug, and worm. In instances of underrepresented species within the dataset, we have implemented straightforward yet potent image augmentation techniques to enhance data quality. BioAuxdataset stands as a valuable resource for the detection and identification of these organisms, leveraging the power of deep learning algorithms to unlock new potentials in ecological research and beyond. This dataset not only enriches the academic discourse but also opens up avenues for practical applications in agriculture, environmental science, and biodiversity conservation.

Ground spiders (Chelicerata, Araneae) of an urban green space: intensive sampling in a protected area of Rome (Italy) reveals a high diversity and new records to the Italian territory.

Background: Urbanisation is a rapidly growing global phenomenon leading to habitat destruction, fragmentation and degradation. However, urban areas can offer opportunities for conservation, particularly through the presence of green spaces which can even provide important habitats for imperilled species. Spiders, which play crucial roles in ecosystem functioning, include many species that can successfully exploit urban environments. Placed in the middle of the Mediterranean global biodiversity hotspot, Italy possesses an exceptionally rich spider fauna, yet comprehensive data on urban spider communities are still limited. More information on urban spiders in Italy would be extremely beneficial to support conservation efforts, especially in central and southern Italy, where knowledge on the spider fauna is largely incomplete. New information: The current study focused on the spider diversity of a large protected area (Appia Antica Regional Park) in urban Rome, Italy. A total of 120 spider species belonging to 83 genera and 28 families were identified, with 70 species being new records to the Province of Rome, 39 to the Latium Region and two (Pelecopsisdigitulus Bosmans & Abrous, 1992 and Palliduphantesarenicola (Denis, 1964)) to Italy.Forty-one species were recorded during autumn/winter sampling and 107 in spring/summer. The spider fauna recorded from the study area included about 37% of the total spider fauna known from the Province of Rome, 28% of that of the Latium Region and 7% of the entire Italian territory. The most represented families in terms of species richness were Gnaphosidae and Linyphiidae, which accounted for more than 40% of the sampled fauna. Lycosidae were the most abundant family (29% of captured individuals), followed by Zodariidae (16% of captured individuals), Linyphiidae (13% of captured individuals) and Gnaphosidae (7.5% of captured individuals). From a biogeographical point of view, most of the collected species belonged to chorotypes that extend for large areas across Europe and the Mediterranean. The research highlights the role of urban green spaces as refuges for spiders and the importance of arachnological research in urban areas as sources of information on spider biodiversity at larger scales.

Prevalence and molecular characterization of Giardia duodenalis in companion dogs, domestic livestock and wildlife in the Jordan Basin, Israel.

Giardiasis is a small intestinal disease caused by the zoonotic parasite, Giardia duodenalis. This study presents the molecular findings of G. duodenalis infection in companion dogs, domestic livestock and wildlife in the Northern Jordan Basin, Israel. Identification of G. duodenalis was accomplished by nested PCR (nPCR) targeting the 18S rRNA gene. Samples were collected from water (five samples from four sources of which one was recycled water), as well as feces from wolves (Canis lupus) (n = 34), jackals (Canis aureus) (n = 24), wild boars (Sus scrofa) (n = 40), cattle (Bos taurus) (n = 40), dogs (Canis lupus familiaris) (n = 37) and nutria (Mayocastor coypus) (n = 100). All positive samples were sequenced and a phylogenetic tree was drawn using the Bayesian Inference (BI) algorithm. Differences in G. duodenalis prevalence between the different hosts were analyzed by Pearson's chi-square (p < 0.05). Of the total 275 fecal samples, 36 were positive for G. duodenalis (13%). Frequency rates among different animal species was highest in wolves (32.3%), whilst rates in wild boars (22.5%), dogs (16.2%), cattle (12.5%) and jackals (4.2%), were observed to be significantly lower (p < 0.001). Three out of 5 recycled water (RW) samples were G. duodenalis positive. Three clusters with high posterior probabilities (PP) were found in the BI: Cluster 1: samples from wolves, wild boars, water and cattle together with database sequences of assemblages A, B and F, Cluster 2: samples from dogs, nutria and a jackal with sequences from assemblage D and Cluster 3: samples from cattle, wild boars, wolves and dogs with sequences from assemblage C and D. We suggest that wolves serve as reservoirs of G. duodenalis in this region. The finding of Giardia in RW suggests that this vehicle may further contaminate crops intended for human consumption as this water source is used for agricultural irrigation.

Further records of social parasitic ants in Europe and review of the Bulgarian species.

Background: Social parasitic ants exploit the colonies of other ant species, either permanently or temporarily. The permanent parasites are amongst the rarest species of ants, although their hosts may be very common. Due to their rarity and often restricted distribution range, most of them are listed as vulnerable. Filling in the gaps in geographical and host ranges will advance our understanding of the social parasitic lifestyle's origin and evolution. New information: This study reports the first records of the slave-makers Myrmoxenusalgerianus (Cagniant, 1968) for Europe (Italy, Sicily) and M.ravouxi (André 1896) for Albania and the inquiline Anergatesatratulus (Schenck, 1852) for Malta (Gozo). We also report new localities of Camponotusuniversitatis Forel, 1890 for Albania and Myrmoxenusgordiagini Ruzsky, 1902, M.kraussei (Emery, 1915) and Anergatesatratulus for Bulgaria. Diversity, type of parasite-host relationships, host range, distribution and conservation of social parasitic ant species in Bulgaria are discussed. Although social parasitic ants are still understudied in Bulgaria, they represent 21% of the regional ant fauna.

Larval and adult morphology of Photuriselliptica Olivier (Coleoptera, Lampyridae) and a Halloweeny case of cave-dwelling firefly larva feeding on bat guano.

The predatory firefly Photuriselliptica is common throughout the Atlantic Forest and has been proposed as a biomonitor due to the species' narrow niche and elevational range. However, the species is only known from adults, and a more effective monitoring of its populations hinges on the lack of knowledge on their immature stages. Recent sampling in ferruginous caves and inserted in other lithologies, on sites in the Atlantic Forest and Cerrado, have led to the capture of firefly larvae later reared to adults in the lab. Firefly larvae have been reported in South American caves before; however, they have only been identified to family due to the adult-biased taxonomy of Lampyridae. Here, we provide an updated diagnosis of Photuriselliptica, describe its immature stages for the first time, and update the distribution of the species. The larvae of Photuriselliptica were observed to interact with guano of several bat species, including that of vampire bats. These observations are consistent with the less specialized feeding preferences of photurine larvae, unlike most other firefly taxa, which specialize in gastropods and earthworms. It is yet unclear whether P.elliptica are cave specialists. However, since its occurrence outside caves remains unknown, protecting cave environments must be considered in conservation strategies for this important biomonitor species.

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As regulators of the surface land processes, soil fauna communities are the vital foundations for healthy terrestrial ecosystems. Soil fauna have been studied in China for more than 70 years. Great progresses have been achieved in exploring soil fauna species composition and geographical distribution patterns. Soil fauna eco-geography, as a bridge between soil fauna geographic patterns and ecosystem services, has a new development opportunity with the deep recognition of soil fauna ecological functions. Soil fauna eco-geography research could be partitioned into four dimensions including the spatio-temporal patterns of: 1) the apparent characteristics of soil fauna community, such as species composition, richness and abundance; 2) the intrinsic characteristics of soil fauna community, such as dietary and habits; 3) soil fauna-related biotic and abiotic interactions especially those indicating drivers of soil fauna community structure or shaping the roles of soil fauna in ecosystems; and 4) soil fauna-related or -regulated key ecological processes. Current studies focus solely on soil fauna themselves and their geographical distributions. To link soil fauna geography more closely with ecosystem services, we suggested that: 1) converting the pure biogeography studies to those of revealing the spatio-temporal patterns of the soil fauna-related or regulated key relationships and ecological processes;2) expanding the temporal and spatial scales in soil fauna geographical research;3) exploring the integrated analysis approach for soil fauna-related data with multi-scales, multi-factors, and multi-processes;and 4) establishing standard reference systems for soil fauna eco-geographical researches. Hence, the change patterns of ecological niche of soil fauna communities could be illustrated, and precision mani-pulations of soil fauna communities and their ecological functions would become implementable, which finally contributes to ecosystem health and human well-being.

New records for the Helius Lepeletier & Serville fauna (Diptera, Limoniidae) of China.

Background: The genus Helius Lepeletier & Serville, 1828 is globally distributed with 232 species and subspecies, of which 25 have been known to occur in China. Amongst the Chinese Helius crane flies, 24 species are distributed in southern China. The species diversity of Helius in other Chinese regions may be severely underestimated due to a lack of investigation. Some investigations on crane flies in Inner Mongolia, China have been initiated by the authors together with other entomologists, with Helius being one of the key targets of attention. New information: Two Helius species, H. (Helius) flavus (Walker, 1856) and H. (H.) gracillimus Alexander, 1938, are added to the Chinese fauna. The two newly-recorded species also represent the first records of the crane fly tribe Elephantomyiini in Inner Mongolia. Re-descriptions and illustrations of the two newly-recorded species are presented.

Ameripathidae, a new family of antipatharian corals (Cnidaria, Anthozoa, Hexacorallia, Antipatharia).

A new family of antipatharian corals, Ameripathidae (Cnidaria: Anthozoa: Antipatharia), is established for Ameripathespseudomyriophylla Opresko & Horowitz, gen. et sp. nov. The new family resembles Myriopathidae and Stylopathidae in terms of the morphology of the polyps and tentacles and the pinnulate branching of the corallum. Phylogenetic analysis using a genomic data set of 741 conserved element loci indicates that the new family is sister to a clade containing the Myriopathidae, Stylopathidae, Antipathidae, and Aphanipathidae.

Critical role of benthic fauna in enhancing nanoplastics removal in constructed wetland: Performance, fate and mechanism.

The presence of nanoplastics (NPs) in wastewater poses a considerable risk to ecosystems. Although constructed wetlands (CWs) have the potential to removal NPs, their efficiency is limited by insufficient consideration of ecosystem integrity. Herein, three typical benthic fauna (Corbicula fluminea, Chironomus riparius and Tubifex tubifex) were added to CWs to improve the ecological integrity of CWs, and further enhance the ecological benefits. Results indicated that the addition of C. fluminea, C. riparius and T. tubifex increased NPs removal by 19.14 %, 17.02 %, and 15.76 % than that without benthic faunas, respectively. Based on fluorescence signal analysis, the presence of benthic fauna could intake NPs, and enhanced the adsorption of NPs by plants. The addition of C. fluminea significantly increased catalase (1541.82 ± 41.35 U/g), glutathione S-transferase (0.34 ± 0.02 U/g), and superoxide dismutase (116.33 ± 6.91 U/g) activities (p < 0.05) as a defense mechanism against NPs-induced oxidative stress. Metagenomic analysis revealed that the abundances of key enzymes involved in glycolysis, the tricarboxylic acid cycle, and polystyrene metabolism pathways were increased when C. fluminea was added, corresponding to the microbial degradation of NPs. Overall, the results of this study implied that the benthic fauna can efficiently remove NPs from wastewater in CWs.

Fauna and Landscapes of the Southern Minusinsk Basin (Southern Siberia) at the End of the Late Pleistocene.

Paleolandscapes of the first half of MIS 2 or the Last Glacial Maximum (LGM) were reconstructed based on the study of the first mammoth fauna locality found in the Abakan River valley and a comparison with other localities of the South Minusinsk Basin. Sediments of the Uytag geological section under study included silt interlayers underlain and overlain by sandstone rock slack fragments and were interpreted as weakly eroded diluvium. Studies of the Uytag locality made it possible to clarify the time and range of distribution in southern Siberia for several mammals (Ovis ammon, Equus ferus, Marmota baibacina, Sicista subtilis, Lagurus lagurus, and Microtus gregalis) and the Pleistocene bird Aquila chrysaetos. The age of the locality was confirmed by radiocarbon dating. The species composition of the Uytag fauna was similar to that of other localities of the same age and region. All known localities and single finds of faunal remains indicate that open steppe landscapes were widely developed in the South Minusinsk Basin during the LGM.

Assessment of heavy metals in soil, leaf litter, and their bioaccumulation in terrestrial macroinvertebrates in Sohag Governorate, Egypt.

The presence of elevated levels of heavy metals in soil poses a significant environmental concern with implications for human health and other organisms. The main objective of our study was to reduce the gap information of seasonal abundance, distribution of heavy metals in soil, leaf litter, and some macroinvertebrates in a citrus orchard (Citrus sinensis) in Sohag Governorate, Egypt. The heavy metals copper (Cu), zinc (Zn), lead (Pb), and cadmium (Cd) were determined by atomic absorption spectrometry. Degree of contamination (DC) was determined for both soil and leaf litter contamination. However, the bioaccumulation factor (BAF) was estimated to determine metal accumulation in the macroinvertebrates including earwigs Anisolabis maritima, chilopoda Scolopendra moristans, spider Dysdera crocata, and earthworm Aporrectodea caliginosa. The study area had clay-loam with varying organic matter, salinity, and pH levels. The degree of contamination varied among seasons, with the highest levels typically observed in autumn in both soil and leaf litter. The soil ranged from low contamination (1.82) to high contamination levels (4.4), while the leaf litter showed extremely high (30.03) to ultra-high (85.92) contamination levels. The mean ecological risk index results indicated that the sampling area had moderate ecological risk levels for Cd (44.3), Zn (42.17), and Pb (80.05), and extremely high levels for Cu (342.5). Heavy metal concentrations in the selected fauna were the highest in autumn, and the bioaccumulation factor varied among species and seasons with some species classified as e-concentrators, micro-concentrators, and macro-concentrators of certain heavy metals. Scolopendra moristans exhibited the highest mean metal concentrations (Cd, Pb, and Zn), while Aporrectodea caliginosa had the lowest. Thus, the differences in heavy metal concentrations found in different soil taxa highlight the significance of taxing a holistic understanding of feeding mechanisms into account when evaluating the potential risk for animals that consume invertebrates.

Seasonal Dynamics of Fruit Flies (Diptera: Drosophilidae) in Natural Parks of Moscow City, Russia.

The insect fauna of natural parks in large cities has not been sufficiently studied in Russia. This study represents the first investigation of the seasonal dynamics and species diversity of Drosophilidae in Moscow city. Traps with fermenting liquid were placed on the ground under trees to collect flies from four natural park sites between early May and late September from 2021 to 2023. A total of 26,420 individuals belonging to 11 genera and 33 drosophilid species were identified, with 21 species from 6 genera being new to the fauna of Moscow. Drosophila obscura Fll., D. phalerata Mg., and D. testacea Roser were the most abundant species in the traps. Peaks in the abundance of drosophilids varied between years, but the lowest abundance was always observed in May. In 2022, the highest number of flies was collected (9604 specimens), with slightly fewer in 2023 (8496 specimens), and even fewer in 2021 (8320 specimens). In 2022, the highest species diversity of drosophilids was also recorded-33 species-while 28 species were found in both 2021 and 2023. The high variability in the abundance of individual drosophila species obscures the differences between the studied years due to the effects of the "Month" and "Site" factors. The diversity metrics exhibit similar patterns among drosophila communities inhabiting comparable biotopes. Specific climatic factors, such as the temperature and precipitation, impact the species abundance and community diversity indices primarily through their effects on the preimaginal stages of drosophila development. For several species, the population dynamics in the spring, post-hibernation, are influenced by the conditions preceding winter.

Age and sex effects on histological features and in vitro culturing of Antillean manatee (Trichechus manatus manatus, Linnaeus 1758) dermis.

The biobanks from dermal biopsies represent an interesting strategy for biodiversity conservation. Nevertheless, the morphological and cellular patterns of the dermis can be influenced by the age and sex of the individual. Therefore, evaluating these factors is interesting for forming biobanks of Antillean manatees. These animals, representatives of marine fauna, have had their population reduced, and biobanks are essential for their conservation. Then, we evaluated the effects of age (3.5 years vs. 3.6-16 years vs. 23.6 years) and sex (males vs. females) on morphological and cellular parameters using histological and in vitro culture techniques. Regardless of age, no differences were observed for dermal thickness, collagen fibres, tissue proliferative activity and viable cell recovery. Nonetheless, fibroblast reduction was observed in groups aged 23.6 years compared to other animals (p < 0.05). Additionally, cells from animals aged 3.6-16 years showed more significant mitochondrial damage than the other groups (p < 0.05). Regardless of sex, no differences were observed for dermal thickness, collagen fibres, tissue proliferative activity and viable cell recovery; however, females had fewer fibroblasts than males (p < 0.05). Cells from females showed lower mitochondrial damage when compared to cells from males. In summary, although age and sex do not influence dermal thickness and cell recovery, variations in the number of fibroblasts and mitochondrial characteristics were observed among the groups. These differences may be significant for understanding the dermis aspects to be correlated to biobank systems.

Cestode diversity in shrews from islands in the Sea of Japan and the Sea of Okhotsk.

A comparative analysis of taxonomic diversity on shrew cestodes among four islands in the Sea of Japan and the Sea of Okhotsk (Sakhalin, Kunashir, Hokkaido, and Moneron) was performed. Cestode species shared among the islands were identified and their host specificity was investigated. On Sakhalin Island, 33 species of the families Hymenolepididae, Dilepididae and Mesocestoididae were recorded in four shrew species (Sorex caecutiens, S. gracillimus, S. minutissimus and S. unguiculatus). In S. caecutiens, S. gracillimus, and S. unguiculatus on Kunashir Island, 22 species of the same families were found and, on Hokkaido Island, 23 species of the families Hymenolepididae and Dilepididae were recorded. On Moneron Island, three species of cestodes were registered in S. tundrensis. The Sakhalin-Hokkaido-Kunashir complex of shrew cestodes includes eastern-Palearctic, trans-Palearctic and endemic species. High endemism (~22%) of shrew tapeworms in the Sakhalin-Kunashir-Hokkaido Islands was noted as compared to continental territories. The different numbers of cestode species in S. unguiculatus (31), S. caecutiens (29), S. gracillimus (19) and S. minutissimus (1) were found. It was concluded that the cestodes species diversity of shrews of Sakhalin-Kunashir-Hokkaido depended primarily on the history of island formation, their modern physical and geographical features, the abundance of definitive and intermediate cestodes hosts and, to a lesser extent, on the size and remoteness of the islands from the mainland and the diversity of host species.