Compensatory effects of other olfactory genes after CRISPR/cas9 editing of BmOR56 in silkworm, Bombyx mori.

Bombyx mori is an oligophagous economic insect. Cis-Jasmone is one of the main substances in mulberry leaf that attract silkworm for feeding and BmOR56 is its receptor. Potential interaction ways between BmOR56 and cis-Jasmone were explored, which included some crucial amino acids such as Gln172, Val173, Ser176, Lys182, His322, and Arg345. BmOR56 was edited using CRISPR/cas9 for Qiufeng, and a homozygous knockout strain QiufengM was obtained. Compared with Qiufeng, the feeding ability of QiufengM on mulberry leaf did not change significantly, but on artificial diet decreased significantly. QiufengM also showed a dependence on the concentration of mulberry leaf powder. The result indicated that other olfactory genes had a compensatory effect on the attractance of mulberry leaf after the loss of BmOR56. Transcriptome analysis of antennae showed that many genes differentially expressed between Qiufeng and QiufengM, which involved in olfactory system, glucose metabolism, protein metabolism, amino acid metabolism, and insect hormone biosynthesis. Particularly, BmIR21, BmOR53 and BmOR27 were significantly up-regulated, which may have a compensatory effect on BmOR56 loss. In addition, detoxification mechanism was activated and may cause the passivation of feeling external signals in silkworm.

Diverse material properties and morphology of moth proboscises relates to the feeding habits of some macromoth and other lepidopteran lineages.

Insects have evolved unique structures that host a diversity of material and mechanical properties, and the mouthparts (proboscis) of butterflies and moths (Lepidoptera) are no exception. Here, we examined proboscis morphology and material properties from several previously unstudied moth lineages to determine if they relate to flower visiting and non-flower visiting feeding habits. Scanning electron microscopy and three-dimensional imaging were used to study proboscis morphology and assess surface roughness patterns on the galeal surface, respectively. Confocal laser scanning microscopy was used to study patterns of cuticular autofluorescence, which was quantified with colour analysis software. We found that moth proboscises display similar autofluorescent signals and morphological patterns in relation to feeding habits to those previously described for flower and non-flower visiting butterflies. The distal region of proboscises of non-flower visitors is brush-like for augmented capillarity and exhibited blue autofluorescence, indicating the possible presence of resilin and increased flexibility. Flower visitors have smoother proboscises and show red autofluorescence, an indicator of high sclerotization, which is adaptive for floral tube entry. We propose the lepidopteran proboscis as a model structure for understanding how insects have evolved a suite of morphological and material adaptations to overcome the challenges of acquiring fluids from diverse sources.

The pollution characteristics and risk assessment of microplastics in mollusks collected from the Bohai Sea.

Microplastics (MPs) pollution in the marine environment has become a global problem. In this study, a number of 21 mollusk species (n = 2006) with different feeding habits were collected from 11 sites along the Bohai Sea for MPs uptake analysis. The MPs in mollusk samples were isolated and identified by micro-Fourier Transform Infrared Spectroscopy (µ-FTIR), and an assessment of the health risks of MPs ingested by mollusk consumption is also conducted. Approximately 91.9 % of the individuals among all the collected species inhaled MPs, and there was an average abundance of 3.30 ± 2.04 items·individual-1 or 1.04 ± 0.74 items·g-1 of wet weight. The shape of MPs was mainly fiber, and a total number of 8 polymers were detected, of which rayon had the highest detection rate (58.3 %). The highest abundance, uptake rate and polymer composition of MPs was observed in creeping types, suggesting that they might ingest these MPs from their food. The gastropod Siphonalia subdilatata contains the highest levels of MPs, which may increase the risk of human exposure if consumed whole without removing the digestive gland. The polymer risk level of MPs in these mollusks was Level III (H = 299), presenting harmful MPs such as polyvinyl chloride. In terms of human exposure risk, the average risk of human exposure to MPs through consumption of Bohai mollusks is estimated to be 3399 items·(capita·year)-1 (424-9349 items·(capita·year)-1). Overall, this study provides a basis for the ecological and health Risk assessment of MPs in mollusks collected from the coastline of China.

Both host and diet shape bacterial communities of predatory mites.

Microbial communities, derived from food, ambient, and inner, can affect host ecological adaption and evolution. Comparing with most phytophagous arthropods, predators may have more opportunities to develop specific microbiota depending on the level of prey specialization. To explore how diet sources affect host microbial communities and vary across predator species, we considered 3 types of predators from Phytoseiidae (Acari: Mesostigmata): polyphagous (Amblyseius orientalis Ehara, Neoseiulus barkeri Hughes, and Amblyseius swirskii Athias-Henrio), oligophagous (Neoseiulus californicus McGregor), and monophagous (Phytoseiulus persimilis Athias-Henriot) predatory mites. The polyphagous species were fed on 2 types of diets, natural prey and alternative prey. By using 16S rRNA sequencing, we found that diet was the main source of microbiota in predatory mites, while there was no clear pattern affected by prey specialization. Among 3 polyphagous predators, host species had a larger impact than prey on microbial composition. Unlike A. orientalis or N. barkeri which showed consistency in their microbiota, prey switching significantly affected ß-diversity of bacterial composition in A. swirskii, with 56% of the microbial alteration. In short, our results confirmed the substantial influence of diet on host microbial construction in predatory species, and highlighted species differences in shaping the microbiota which are not necessarily related to prey specialization.

Assessment of microplastic contamination in the gastrointestinal tracts of indigenous fishes from north eastern hill regions of Bhogdoi, a tributary of River Brahmaputra, India.

Microplastic (MP) pollution in freshwater environments has drawn considerable attention over the decades due to the risk posed by MPs to the aquatic fauna and human health. In this study, the occurrence of MPs was assessed from the gastrointestinal tracts (GIT) of indigenous fishes from Bhogdoi River, a tributary of River Brahmaputra, northeast hill region, Assam, India. All the fish species (n = 180) analysed showed 100% occurrence of MPs sampled from three stations of River Bhogdoi. A total of 488 microplastic particles were recorded from GIT of fishes collected. The mean abundance of MPs was highest in Station-II (7.64 ± 2.77 numbers individual-1) with lowest in S-I (3.17 ± 0.99 numbers individual-1). The MP size of 100-500 µm, MP shape of fibre (43.33%) and fragments (35.33%), blue colour MPs (26-37%) and polymer type PA (23-30%) were most prevalent in fishes analysed from River Bhogdoi. The mean abundance of MPs was higher in herbivore fishes (8.52 ± 1.22 numbers individual-1) as compared to carnivorous and omnivorous fish species with highest abundance in Labeo rohita (12.11 ± 2.13 numbers individual-1). Fibre and fragments with size range of 100-500 µm were dominant among all the feeding habits. Blue and black colour MPs were abundant among herbivore and carnivore fishes. Fourier transform infrared spectroscopy results confirmed that polyamide and polypropylene were the most abundant MP polymer in the fishes analysed. The present study provides baseline information of MPs in River Bhogdoi, Assam, for its future monitoring and assessment of contaminants.

Interspecific differences in plasma carotenoid profiles in nestlings of three sympatric vulture species.

Carotenoids are diet-based micronutrients important in health and coloration signaling. Related species with similar diets can differ in the kinds and levels of circulating carotenoids, which suggests specific physiological mechanisms to efficiently utilize these micronutrients, regardless of their availability. We explored whether diet and parental provisioning of unusual sources of carotenoids (fresh vegetal matter and vertebrate feces) can explain the occurrence and concentrations of carotenoids in the cinereous vulture Aegypius monachus, griffon vulture Gyps fulvus, and Egyptian vulture Neophron percnopterus nestlings, even when these pigments appear to not be deposited in their integumentary system. A greater diversity of wild prey in diet could be behind the profile of higher concentrations of carotenoids in the Egyptian vulture, the species with carotenoid-dependent coloration during adulthood, while differences in diet composition between cinereous and griffon vultures do not translate to different carotenoid profiles. The carotenoid profile appears to not be related to the ingestion of unusual matter rich in these compounds, although the infrequent occurrence of lycopene and unidentified γ-carotene-like compounds suggest that these vultures may be exploiting vegetal matter that left no identifiable unconsumed remains in the nest of Egyptian vultures. The consumption of green plant material by griffon vultures does not result in especially high levels of carotenoids when compared to the carotenoids found in cinereous vultures, which do not consume green plant material. Ungulate feces were not provisioned to Egyptian vulture nestlings, despite the fact they contain carotenoids that adults need for appropriate coloration. Overall, this study indicates that diet differences alone appear insufficient to explain contrasting interspecific carotenoid profiles, especially since all types of food consumed are considered to be poor in carotenoids, except vegetable matter. We suggest that nestling Egyptian vultures are comparatively efficient in uptaking carotenoids present in low concentrations in food when these compounds are not deposited in their integument, which suggests allocation to other functions.

High resolution in turbid waters: Ampullae of lorenzini in the daggernose shark Carcharhinus oxyrhynchus (Valenciennes, 1839) (Elasmobranchii: Carcharhinidae).

The present work aimed to analyze the distribution of the electrosensory pores of the Daggernose Shark Carcharhinus oxyrhynchus identifying the organ's importance in the natural history of the species. By examining photographs and digital microscope videos, we found that C. oxyrhynchus possesses the highest abundance of pores among Carcharhiniformes. This suggests a well-developed electroreceptor system, which may have maximized its evolutionary success in high turbidity environments. Furthermore, as a morphologically derived species, C. oxyrhynchus comprises a more complex and specialized electrosensory system. Notably, the species exhibits ontogenetic variation in pore abundance, highlighting the importance of a high-resolution system for adults. The higher density of pores in the ventral region indicates a preference for benthic prey, despite also feeding on pelagic items. Moreover, the species has a high-resolution electrosensory system and a high density of pores in the snout, which emphasizes the importance of the elongated snout that expands the electroreception search area coverage. Evolutionary convergence was observed in the development of the electrosensory system, as C. oxyrhynchus shares characteristics of pore distribution and abundance with phylogenetically unrelated species.

Effects of social confinement during the first wave of COVID-19 in Mexico City.

Background: The COVID-19 pandemic led to global social confinement that had a significant impact on people's lives. This includes changes such as increased loneliness and isolation, changes in sleep patterns and social habits, increased substance use and domestic violence, and decreased physical activities. In some cases, it has increased mental health problems, such as anxiety, depression, and post-traumatic stress disorder. Objective: The objective of this study is to analyze the living conditions that arose during social confinement in the first wave of COVID-19 within a group of volunteers in Mexico City. Methods: This is a descriptive and cross-sectional analysis of the experiences of volunteers during social confinement from 20 March 2020 to 20 December 2020. The study analyzes the impact of confinement on family life, work, mental health, physical activity, social life, and domestic violence. A maximum likelihood generalized linear model is used to determine the association between domestic violence and demographic and health-related factors. Results: The findings indicate that social confinement had a significant impact on the participants, resulting in difficulties within families and vulnerable conditions for individuals. Gender and social level differences were observed in work and mental health. Physical activity and social life were also modified. We found that suffering from domestic violence was significantly associated with being unmarried (OR = 1.4454, p-value = 0.0479), lack of self-care in feeding habits (OR = 2.3159, p-value = 0.0084), and most notably, having suffered from a symptomatic COVID-19 infection (OR = 4.0099, p-value = 0.0009). Despite public policy to support vulnerable populations during confinement, only a small proportion of the studied population reported benefiting from it, suggesting areas for improvement in policy. Conclusion: The findings of this study suggest that social confinement during the COVID-19 pandemic had a significant impact on the living conditions of people in Mexico City. Modified circumstances on families and individuals, included increased domestic violence. The results can inform policy decisions to improve the living conditions of vulnerable populations during times of social confinement.

Accelerated recovery of lake macroinvertebrates in the third decade since the reversal of acidification.

Chemical reversal from acidification has been progressing in European freshwaters since the late 1980s, responding to successful control of atmospheric pollution by acidifying emissions. However, biological recovery is often delayed after improvements in water composition. We studied macroinvertebrate recovery from acidification in eight glacial lakes in the Bohemian Forest (central Europe) between 1999 and 2019. The chemical composition of these lakes reflects a complex of environmental changes, dominated by a very steep decline in acid deposition and, currently, by elevated nutrient leaching following climate-induced tree dieback within their catchments. Temporal trends in species richness, abundance, species traits and community composition were evaluated with regard to water chemistry, littoral habitat properties and fish colonisation. The results showed accelerated recovery of macroinvertebrates following two decades of gradual improvement in water composition and slowly progressing biological rehabilitation. We observed a significant increase in macroinvertebrate species richness and abundance, coupled with distinct changes in community composition, the extent of changes varying between lakes, reflecting different littoral habitat properties (vegetated vs. stony) and water chemistry. Overall, the communities shifted toward more specialised (grazers, filterers, and phytophilous species) and acid-tolerant taxa at the expense of detritivorous, eurytopic and acid-resistant taxa. Where fish reappeared, open-water taxa declined greatly. Compositional changes were likely driven by the combined effects of water chemistry reversal, rehabilitation of habitat conditions and fish colonisation. Despite favourable trends, communities in recovering lakes still lack several biotic elements, particularly less vagile, acid-sensitive taxa and specialised herbivores known from the regional species pool. It is expected that future progress in lake recovery will be further promoted or inhibited by stochastic colonisation or disturbance events.

Transcriptome analysis of anorexic and preferred silkworms (Bombyx mori) on artificial diet.

The silkworm, Bombyx mori, is an important oligophagous economic insect and feeding habits of different silkworm varieties to artificial diet are different. Research on the mechanisms of feeding habits on artificial diet, and breeding of silkworm varieties adapted on artificial diet, which is a necessary condition for industrial silkworm rearing, is currently lacking. For an artificial diet, Xin was anorexic, whereas Haoyue A showed a strong appetite. When the two varieties were crossed, the F1 generation showed a poor appetite for the artificial diet and had a setae dispersion rate of <50 %. However, the F2 generation, self-bred progeny of F1, had a good appetite for the artificial diet, with a setae dispersion rate of 70 %. Herein, transcriptome analysis was conducted on the F2 generation, comparing individuals with anorexic and preferred feeding habits, and 2188 differential genes were identified, with 1524 genes up-regulated and 934 genes down-regulated. Several genes were identified to contribute to feeding habits, such as genes involved olfactory system, energy supply, and cell proliferation and differentiation. GO enrichment revealed a large number of DEGs related to behavior, growth, signaling, developmental process, response to stimulation, and other pathways. Furthermore, proteins closely related to feeding were expressed differently. Some DEGs were selected for qRT-PCR, and results indicated the reliability of the DEG results. The DEGs between individuals with anorexic and preferred feeding habits were screened by RNA-Seq technology, which provides a reliable reference to study molecule mechanisms of feeding habits on artificial diet.

Trophic niche influences ingestion of micro- and mesoplastics in pelagic and demersal fish from the Western Mediterranean Sea.

Plastic pollution has been extensively documented in the marine food web, but targeted studies focusing on the relationship between microplastic ingestion and fish trophic niches are still limited. In this study we investigated the frequency of occurrence and the abundance of micro- and mesoplastics (MMPs) in eight fish species with different feeding habits from the western Mediterranean Sea. Stable isotope analysis (δ13C and δ15N) was used to describe the trophic niche and its metrics for each species. A total of 139 plastic items were found in 98 out of the 396 fish analysed (25%). The bogue revealed the highest occurrence with 37% of individuals with MMPs in their gastrointestinal tract, followed by the European sardine (35%). We highlighted how some of the assessed trophic niche metrics seem to influence MMPs occurrence. Fish species with a wider isotopic niche and higher trophic diversity were more probable to ingest plastic particles in pelagic, benthopelagic and demersal habitats. Additionally, fish trophic habits, habitat and body condition influenced the abundance of ingested MMPs. A higher number of MMPs per individual was found in zooplanktivorous than in benthivore and piscivorous species. Similarly, our results show a higher plastic particles ingestion per individual in benthopelagic and pelagic species than in demersal species, which also resulted in lower body condition. Altogether, these results suggest that feeding habits and trophic niche descriptors can play a significant role in the ingestion of plastic particles in fish species.

Scanning Electron Microscopy of Antennae and Mouthparts of Mezira yunnana Hsiao (Hemiptera: Aradidae): Specialized Microstructures Reflecting Adaptation to Mycetophagy.

Many species of the family Aradidae (also known as flat bugs) feed on fungal mycelia and fruiting bodies. In order to better understand the morphological adaptation to this unique feeding habit, we examined the microstructure of antennae and mouthparts of an aradid species, Mezira yunnana Hsiao, using scanning electron microscope, and documented the fungal feeding process under laboratory conditions. The antennal sensilla include three subtypes of sensilla trichodea, three subtypes of sensilla basiconica, two subtypes of sensilla chaetica, sensilla campaniformia, and sensilla styloconica. The apex of the second segment of flagellum has a large number of various sensilla forming a sensilla cluster. The labial tip is distally constricted, which is rarely observed in other Pentatomomorpha species. The labial sensilla include three subtypes of sensilla trichodea, three subtypes of sensilla basiconica, and a sensilla campaniformia. The tip of the labium has only three pairs of sensilla basiconica III and small comb-shaped cuticular processes. The external surface of the mandibular apex has 8-10 ridge-like central teeth. A series of key morphological structures associated with mycetophagous feeding habit were identified, which will facilitate future studies on adaptive evolution of species in Pentatomomorpha as well as in other heteropteran lineages.

Escala de Influência Psicossocial da ingestão de Frutas, Verduras e Legumes do Adolescente: Adaptação e Validade Fatorial / Psychosocial Influence Scale of Fruit and Vegetable Consumption among Adolescents: Adaptation and Factorial Validity

Resumo Instrumentos para avaliar determinantes psicossociais da ingestão de frutas, verduras e legumes (FLV) de adolescentes são escassos, e há um instrumento - sem nome e validação - que investiga estes determinantes oriundos de teorias da psicologia social e direcionado à frequência de ingestão semanal de FLV de adolescentes. O objetivo foi apresentar o processo de adaptação e validação fatorial deste instrumento para ser aplicado em estudos na população brasileira. Realizou-se equivalência conceitual e de itens; equivalência semântica por tradutores e especialistas e semântica e operacional por entrevistas dirigidas com público-alvo. A versão final - denominada Escala de Influências Psicossociais da ingestão de Frutas, Verduras e Legumes de Adolescentes (PSI-FAVES) - foi testada com 429 estudantes (58% mulheres) utilizando confiabilidade por ômega de McDonald (ω) e Análise Fatorial Confirmatória (AFC) como métodos analíticos. A versão final demandou refinamento e o instrumento apresentou confiabilidade total adequada (ω=0,86) e bom ajuste dos dados ao modelo previamente conjecturado (CFI=0,955; TLI=0,951 e RMSEA (90%IC)=0,043 (0,038-0,049), sendo o primeiro instrumento sistematicamente validado para investigar determinantes psicossociais da ingestão de FLV de adolescentes.

Abstract Instruments to evaluate psychosocial determinants of fruit and vegetable (F&V) consumption among adolescents are scarce, and there appears to be only one instrument - unnamed and unvalidated - that investigates determinants derived from theories of social psychology targeting the frequency of F&V consumption among adolescents. The scope of this paper was to present the process of cultural adaptation and factor validation of this instrument al-lowing its use in studies with Brazilian adolescents. The cross-cultural adaptation process was accomplished and the final version - named Psychosocial Influence Scale for Fruit and Vegetable Consumption among Adolescents (PSI-FAVES) - was tested with 429 students (58% female) using McDonald's omega (ω) reliability and Confirmatory Factor Analysis (CFA) as analytical methods. The final version required refinement and the instrument showed adequate overall reliability (ω=0.86) and good fit of the data to the previously elaborated model (CFI=0.955; TLI=0.951 and RMSEA (90%CI) =0.043 (0.038-0.049)), being the first systematically validated instrument to investigate psychosocial determinants of F&V consumption among adolescents.

Feeding habits and foraging patch selection strategy of the giant panda in the Meigu Dafengding National Nature Reserve, Sichuan Province, China.

Despite retaining a digestive system with carnivorous characteristics, the giant panda has now specialized to eat bamboo. Therefore, research on its feeding habits and foraging strategies has always been an important field in the study of giant panda population ecology. Located in the core distribution area of the giant panda population in the Liangshan mountains, the Meigu Dafengding Nature Reserve acts as the link between the major panda reserves in the Liangshan mountains and the key corridor zone connecting the giant panda populations in the east and west. This study was performed in 2017 and aimed to determine the feeding habits of giant pandas in this region, the heterogeneity of food resources in different seasons, and the selection strategy for foraging patches. To achieve this, we used the line transect method to investigate the giant panda population, collected fresh feces, analyzed the nutritional composition of bamboo samples, and set up a sampling grid of foraging patches in different seasonal distribution areas. It was found that wild giant pandas in the region spend most of the year at lower elevations(1939-3296 m) foraging for Yushania maculata and Yushania ailuropodina, and only move to higher elevations(2844-3770 m) in summer for Bashania fangiana. Their preferred foraging patches(n = 65) had certain topographical features, such as gentle slopes(< 25°) and closer proximity to water sources(< 500 m), and the nutritional quality of bamboo resources in foraging patches was significantly better than in those they avoided or rarely used. The food habits and foraging patch selection strategies of giant pandas in the Liangshan and Qinling mountains are relatively similar, and the results of this study can help provide a scientific basis for the conservation and management of wild giant panda habitats in different mountain regions.

Feeding ecology of the Terciopelo pit viper snake (Bothrops asper) in Ecuador.

Thoroughly documenting prey items and diet composition is crucial for understanding a predator's role in the ecosystem. In gape restricted predators, such as snakes, documenting and analyzing the type and size of the prey is important to interpret their ecological role. We describe the diet patterns of a species of venomous snake, the Terciopelo pit viper (Bothrops asper), from its Ecuadorian populations. Examining the gastrointestinal contents of museum specimens collected over an extensive area of the Pacific lowlands of Ecuador, we encountered 69 identifiable prey items from four major taxonomic groups (amphibians, centipedes, mammals, and reptiles). We evaluated the observed composition of prey to check for differences between sexes and size-classes. To complement our observations of the Terciopelo species complex throughout their distribution, we carried out a systematic literature review. Our data show an ontogenetic shift in diet, with a transition from more diverse diet in juveniles towards a mammal-specialized diet in adults, and distinct proportion of prey taxa between the sexes in the juvenile size class.

Flood pulse as a driving force of Pb variation in four fish guilds from Puruzinho Lake (western Amazon).

The Brazilian Amazon is heavily impacted by mining activities, especially by gold and cassiterite mining. Pb is a contaminant released during cassiterite extraction, and it stands out for its high toxicity. The present study aimed to evaluate the effect of flood pulse on Pb dynamics in four fish species with different feeding habits (Cichlapleiozona: carnivorous, Mylossomaduriventre: herbivorous, Prochilodus nigricans: detritivorous, and Serrasalmus rhombeus: piscivorous) over four hydrological periods in Puruzinho Lake (Brazilian Amazon), which receives water from the Madeira River. The risk assessment for daily Pb intake through the consumption of these fish by the local riverside population was also carried out. Fish species were sampled during four Amazonian hydrological periods: rising water, high water, falling water, and low water. Pb and stable isotopes (δ15N and δ13C) measurements were carried out on fish muscle. The flood pulse had the same effect on Pb concentrations of all the fish species regardless of their feeding habits, as we found a nonsignificant interaction between these variables. The Pb in fish muscle increased in the flood season (rising and high water) and decreased in the dry season (falling and low water). The Pb concentration in fish increased significantly with their trophic level (δ15N), evidencing the occurrence of biomagnification of the element along the trophic chain (R2 = 0.24). The main food source of the herbivorous fish (δ13C) was very distinct from that of the others, which had a large overlap in their values. Regarding risk assessment, the daily Pb intake through consumption of the herbivorous species during high water (17.82 ± 19.68 µg∙day-1) exceeded the limit determined by the Food and Drug Administration (FDA) of 12.5 µg∙day-1, representing a health risk to the riverside population of Puruzinho Lake.

Improvement in dietary diversity and feeding habits of malnourished under-five children attending supplementary feeding programmmes: a community-based cross-sectional study in Zambia.

A community-based cross-sectional study was conducted on 390 under-five malnourished children enrolled in the Rainbow Project supplementary feeding programmmes-SFPs. Dietary diversity, feeding habits and nutritional status at admission (T1) and at discharge (T2) were compared. At T1 the diet was monotonous and unbalanced, with a progressive decline in dietary diversity and anthropometric values noted with children's age growth (p < 0.001). Significant improvements were registered at T2: DDS 5.1 ± 1.1 SD vs. 8.3 ± 1.0 SD; meal frequency 3.0 ± 0.6 SD vs. 4.9 ± 0.2 SD; animal-protein consumed 62.8% vs. 90.5%; drinking water treated 41.0% vs. 97.2%. At T1, the risk of having ZMUAC < 2.5SD increased when teenage motherhood (AOR: 5.3; CI: 1.8-15.2; p = 0.002), followed by children's age >2 years (AOR: 1.9; CI: 1.1-3.5; p = 0.020). Children's age was associated with an increased risk of WAZ < 2.5 SD (AOR: 4.9; CI: 2.4-10.4; p < 0.001). When considering inadequate DDS, the variable associated was breastfeeding cessation (AOR: 12.0; CI: 4.6-31.4; p < 0.001). Rainbow's SFPs have proved effective in treating under-five malnourished children, irrespective of the severity of malnutrition.

Fatty Acid Composition of M. Biceps Femoris of Edible Dormouse (Glis glis L.).

This study aimed to investigate the fatty acid (FA) composition of edible dormouse m. biceps femoris in both sexes. More than 20 FA were identified in the muscle, with the 18:1cis-9 (oleic acid) being the most abundant in both sexes, comprising more than 50% of total FA in muscle. The most dominated FA were monounsaturated (MUFA), followed by saturated FA (SFA) and polyunsaturated FA (PUFA), reaching 54.8%, 25.43% and 19.8% of total FA, respectively. Sums of PUFA and n-3 PUFA tended (p > 0.05) to be higher in males than in females. There were no significant differences between sexes on the FA composition. Nevertheless, the 18:2n-6 tended to differ between sexes (p = 0.063). Several long-chain PUFA (LC-PUFA) were detected in dormouse muscle, with the 20:4 n-6 (arachidonic acid, AA) and the 22:6 n-3 (docosahexaenoic acid, DHA) being the most abundant in both sexes. The relatively high stearoyl-CoA desaturase (SCD) indexes and the large concentration of 18:1cis-9 in dormouse muscle tissues might point to a low mobilization of the SCD products. Furthermore, finding the unusual FA 20:3 ∆5,∆11,∆14, suggests feeding on leaf and wood lipids of Coniferophytes. We demonstrated sexual size monomorphism in edible dormouse. The literature regarding the composition of dormouse meat is scarce and no studies reported the FA composition of muscle, thus, this work can contribute to increasing the knowledge on edible dormouse physiology and nutritional traits.

How to improve the diet of toddlers? The feasibility of an online, habit-based intervention targeting parental feeding behaviour.

Background: The diet of toddlers is often not in accordance with dietary recommendations, putting them at risk of poor health outcomes later in life. Parents can struggle to provide their toddler with a healthy diet and interventions are needed. Helping parents to form healthy feeding habits may facilitate healthy feeding behaviours. Therefore, the aim of this study was to establish the feasibility of a 6-week online intervention to build healthy feeding behaviours in parents of toddlers. Methods: Parents and primary caregivers of children aged 2-3 (N = 75) were recruited to complete a 6-week online intervention consisting of 6 modules targeting habit formation, planning, goal setting, self-efficacy, interpersonal influences and picky eating. Demographics and feeding behaviours were measured with self-report at baseline and follow-up. Intervention acceptability and feasibility ratings were also gathered post-intervention. Results: Most participants were recruited online, highly educated and in a relationship. After 6-weeks, 17 participants completed the follow-up questionnaire, 11 of whom indicated that they had completed the whole intervention. Improvements were found for some feeding behaviours. Generally, participants who completed the programme reported that they found it acceptable. Conclusions: This study provides insights for future intervention development. Firstly, an online healthy feeding intervention seems to be acceptable but may need to focus on fewer change techniques. Further, engagement should be improved by including target group members and compulsory reminders. The target behaviours seem to be relevant. Online recruitment seems to be preferable and reaches parents and primary caregivers nationwide.

Morphological characters and feeding habits of insectivorous Kuhl's pipistrelle bat Pipistrellus kuhlii Cretzschmar, 1830, in different riverine habitats.

This study revises the morphological characters of both sexes of Kuhl's pipistrelle, including external, cranial and dental characters. This study also establishes a baseline for the diet of this insectivorous bat species across a variety of habitats in the Nile Valley, where diets have never before been investigated. Our findings of the stomach contentswere not significantly affected despite the different habitats from which the samples were collected. The three most common insect taxa recovered were Hymenoptera, Lepidoptera and Coleoptera. The percentage volume of insect orders in the diet of Kuhl's bat (63 stomachs) consisted of 29% of Hymenoptera, 9% of Coleoptera, 27% of Lepidoptera, 32% unknown materials and unexpected thing of these insectivorous bats was the presences of 3% fruits. A single order did not dominate the diet of this specie because Hymenoptera was the most fed insect orders by frequency of occurrence 87.3%, Lepidoptera by 74.6%, Coleoptera 36.5% and fruits in 4.8% of samples. Also, they may also be fed by other insect orders, as unknown materials were found in all samples.