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1.
Artigo em Espanhol | LILACS-Express | LILACS | ID: biblio-1535128

RESUMO

Introducción: El Perú es endémico al virus linfotrópico T humano tipo 1 (HTLV-1), por esas razones es importante conocer la fiabilidad de las pruebas diagnósticas que se usan en el país, con la finalidad de continuar o no su uso. El objetivo fue evaluar el rendimiento de tres pruebas serológicas ELISA Murex, ELISA Wantai e IFI INS-Perú para la detección de anticuerpos anti HTLV-1 frente a muestras peruanas. El estudio. Las tres pruebas fueron evaluadas frente a 382 sueros: 215 positivos y 167 negativos a HTLV-1 (Gold Standar: inmunoblot). Hallazgos. IFI no presentó falsos positivos, Wantai tuvo más falsos negativos (siete) y Murex más falsos positivos (ocho). Las tres pruebas mostraron resultados superiores a 95% para los parámetros estimados de exactitud diagnóstica. Conclusiones. IFI INS-Perú y ELISA Murex tuvieron buen rendimiento diagnóstico para la detección de anticuerpos contra HTLV-1 y son buenos candidatos para continuar siendo usados en Perú.


Background: Peru is endemic to the human T-lymphotropic virus type 1 (HTLV-1), for these reasons it is important to know the reliability of the diagnostic tests used in the country, in order to continue their use or not. The objective was to evaluate the performance of three serological tests ELISA Murex, ELISA Wantai and IFI INS-Peru for the detection of anti-HTLV-1 antibodies against Peruvian samples. The study. The three tests were evaluated against 382 sera: 215 positive and 167 negative for HTLV-1 (Gold Standard: immunoblot). Findings. IFI had no false positives, Wantai had more false negatives (seven) and Murex more false positives (eight). The three tests showed results above 95% for the estimated parameters of diagnostic accuracy. Conclusions. IIF INS-Perú and ELISA Murex had good diagnostic performance for the detection of antibodies against HTLV-1 and are good candidates to continue being used in Peru.

2.
Ciênc. rural (Online) ; 53(5): 1-7, 2023. ilus, tab
Artigo em Inglês | VETINDEX | ID: biblio-1412890

RESUMO

Tissue culture is an important technique for assessing the influence of light on plant growth. This study evaluated the in vitro cultivation of Ocimum basilicum cultivars under different light sources. First, an experiment was conducted to evaluate the effect of fluorescent and LED (Light Emitting Diode) light sources (yellow, blue, green, and red) on five basil cultivars (Cinnamon, Grecco a Palla, Italian Large, Limoncino, and Maria Bonita), followed by, another experiment, with two LED lights (Growlux and Blue). In vitro basil plants did not exhibit good growth under yellow lamps. The size of the roots of Cinnamon, Grecco a Palla and Limoncino cultivars under the two light conditions were similar. Moreover, the luminous condition of the Growlux lamps generated a greater quantity of leaves and fresh, and dry weight than blue LED lamps for Cinnamon, Grecco a Palla and Limoncino cultivars. Thus, it was concluded that the Growlux lamps promoted a greater amount of basil biomass.


A cultura de tecidos é uma técnica importante para avaliar a influência da luz no desenvolvimento das plantas. O objetivo deste trabalho foi avaliar o cultivo in vitro de cultivares de Ocimum basilicum sob diferentes fontes de luz. Primeiramente, foi avaliado o efeito de fontes de luz fluorescentes e LEDs (Diodo emissores de luz amarela, azul, verde e vermelha) em cinco cultivares de manjericão (Cinnamon, Grecco a Palla, Italian Large, Limoncino e Maria Bonita); e posteriormente o efeito de duas lâmpadas LED (Growlux e Azul). As lâmpadas amarelas não proporcionaram um bom desenvolvimento das plantas de manjericão in vitro. Verificou-se que o tamanho das raízes e massa fresca nas cultivares Cinnamon, Grecco a Palla e Limoncino nas duas condições luminosas foram semelhantes. A condição luminosa das lâmpadas Growlux promoveu maior número de folhas, massa fresca e seca quando comparada com lâmpadas LED azuis nas cultivares Cinnamon, Grecco a Palla e Limoncino. Assim, concluiu-se que as lâmpadas Growlux promovem uma maior quantidade de biomassa de manjericão.


Assuntos
Técnicas In Vitro , Ocimum basilicum/crescimento & desenvolvimento , Ocimum basilicum/efeitos da radiação , Fluorescência
3.
Rev. Paul. Pediatr. (Ed. Port., Online) ; 42: e2022125, 2023. tab, graf
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-1449276

RESUMO

ABSTRACT Objective: The aim of this study was to sum up and characterize all Williams-Beuren syndrome cases diagnosed by fluorescence in situ hybridization (FISH) since its implementation, as well as to discuss FISH as a cost-effective methodology in developing countries. Data source: From January 1986 to January 2022, articles were selected using the databases in PubMed (Medline) and SciELO. The following terms were used: Williams syndrome and In Situ Hybridization, Fluorescence. Inclusion criteria included Williams-Beuren syndrome cases diagnosed by FISH with a stratified phenotype of each patient. Only studies written in English, Spanish, and Portuguese were included. Studies with overlapping syndromes or genetic conditions were excluded. Data synthesis After screening, 64 articles were included. A total of 205 individuals with Williams-Beuren syndrome diagnosed by FISH were included and further analyzed. Cardiovascular malformations were the most frequent finding (85.4%). Supravalvular aortic stenosis (62.4%) and pulmonary stenosis (30.7%) were the main cardiac alterations described. Conclusions: Our literature review reinforces that cardiac features may be the key to early diagnosis in Williams-Beuren syndrome patients. In addition, FISH may be the best diagnostic tool for developing nations that have limited access to new technologic resources.


RESUMO Objetivo: Caracterizar todos os casos de síndrome de Williams-Beuren (SWB) diagnosticados por hibridização in situ fluorescente (FISH) desde sua implementação, assim como discutir a relação custo-benefício da metodologia de FISH em países em desenvolvimento. Fontes de dados: Entre janeiro de 1986 e janeiro de 2022 foi realizada uma busca nas bases de dados PubMed (Medical Literature Analysis and Retrieval System Online — Medline) e Scientific Electronic Library Online (SciELO) usando os seguintes termos: síndrome de Williams e hibridização in situ fluorescente. O critério de inclusão utilizado foi conter a descrição detalhada de caso(s) de SWB por FISH. Apenas estudos escritos em inglês, espanhol e português foram incluídos. Trabalhos que apresentavam sobreposição de síndromes/condições genéticas foram excluídos. Síntese dos dados: Após os processos de inclusão, 64 artigos e 205 indivíduos com SWB diagnosticados por meio do método de FISH foram incluídos. O achado mais frequente entre os indivíduos foi a presença de algum tipo de malformação cardíaca (85,4%). A estenose aórtica supravalvar (62,4%) e a estenose pulmonar (30,7%) foram as alterações cardíacas mais descritas. A maioria dos estudos era proveniente dos continentes Europa, Ásia e América do Norte. Conclusões: A presente revisão de literatura reitera que as malformações cardíacas podem ser a chave para o diagnóstico precoce em pacientes com SWB. Ainda, a técnica de FISH parece ser a melhor ferramenta de diagnóstico para os países em desenvolvimento, cujo acesso às novas tecnologias ainda é escasso.

4.
Ciênc. rural (Online) ; 53(5): e20220030, 2023. tab, graf
Artigo em Inglês | LILACS-Express | VETINDEX | ID: biblio-1394270

RESUMO

ABSTRACT: Tissue culture is an important technique for assessing the influence of light on plant growth. This study evaluated the in vitro cultivation of Ocimum basilicum cultivars under different light sources. First, an experiment was conducted to evaluate the effect of fluorescent and LED (Light Emitting Diode) light sources (yellow, blue, green, and red) on five basil cultivars (Cinnamon, Grecco a Palla, Italian Large, Limoncino, and Maria Bonita), followed by, another experiment, with two LED lights (Growlux and Blue). In vitro basil plants did not exhibit good growth under yellow lamps. The size of the roots of Cinnamon, Grecco a Palla and Limoncino cultivars under the two light conditions were similar. Moreover, the luminous condition of the Growlux lamps generated a greater quantity of leaves and fresh, and dry weight than blue LED lamps for Cinnamon, Grecco a Palla and Limoncino cultivars. Thus, it was concluded that the Growlux lamps promoted a greater amount of basil biomass.


RESUMO: A cultura de tecidos é uma técnica importante para avaliar a influência da luz no desenvolvimento das plantas. O objetivo deste trabalho foi avaliar o cultivo in vitro de cultivares de Ocimum basilicum sob diferentes fontes de luz. Primeiramente, foi avaliado o efeito de fontes de luz fluorescentes e LEDs (Diodo emissores de luz amarela, azul, verde e vermelha) em cinco cultivares de manjericão (Cinnamon, Grecco a Palla, Italian Large, Limoncino e Maria Bonita); e posteriormente o efeito de duas lâmpadas LED (Growlux e Azul). As lâmpadas amarelas não proporcionaram um bom desenvolvimento das plantas de manjericão in vitro. Verificou-se que o tamanho das raízes e massa fresca nas cultivares Cinnamon, Grecco a Palla e Limoncino nas duas condições luminosas foram semelhantes. A condição luminosa das lâmpadas Growlux promoveu maior número de folhas, massa fresca e seca quando comparada com lâmpadas LED azuis nas cultivares Cinnamon, Grecco a Palla e Limoncino. Assim, concluiu-se que as lâmpadas Growlux promovem uma maior quantidade de biomassa de manjericão.

5.
Rev Argent Microbiol ; 54(4): 263-267, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35644768

RESUMO

Acidipropionibacterium acidipropionici is widely used for many applications, such as propionic acid production, cereal silage, and also as probiotic. Due to this plethora of applications, new isolates of A. acidipropionici with improved features are being searched for. These new isolates must be accurately identified, however, most approaches become expensive and time-consuming when the number of isolates is high. On the contrary, fluorescence in situ hybridization allows the affordable, reliable, and rapid identification of microorganisms in pure cultures and environmental and medical samples. Therefore, the aim of this work was to apply a fluorescent in situ hybridization probe for the reliable identification of new A. acidipropionici isolates. To this end, probe Pap446, specific for A. acidipropionici, was validated by hybridization assays with strains of this species from different origins, other species of the same genus or family, and unrelated genera. Eight isolates with propionibacterium characteristics were obtained from milk and feces of cows. Probe Pap446, hybridized only with isolates III and VI. The identity of these isolates was further confirmed by PCR using group and species-specific primers for propionibacteria and 16S rDNA sequencing.


Assuntos
Propionibacterium , Silagem , Bovinos , Animais , Hibridização in Situ Fluorescente , Propionibacterium/genética , Silagem/microbiologia , Especificidade da Espécie
6.
Rev. colomb. reumatol ; 29(2): 107-112, Apr.-June 2022. tab
Artigo em Inglês | LILACS | ID: biblio-1423913

RESUMO

ABSTRACT Introduction: Systemic lupus erythematosus (SLE) is a multisystem autoimmune disease. Patients with SLE exhibit multiple serum autoantibodies, including anti-neutrophil cytoplasmic antibodies (ANCAs). There are two main techniques to detect ANCAs: indirect immunofluorescence (IIF) and enzyme-linked immunosorbent assay (ELISA). In this study, an attempt was made to determine the frequency and clinical associations of ANCAs in patients with SLE. Methods: A cross-sectional study was conducted in a tertiary care hospital in Colombia that included 74 patients with SLE. The presence of ANCAs was assessed using IIF with ethanol-fixed slides, and ELISA was used to detect antibody specificities for myeloperoxidase (MPO)-ANCA and proteinase 3 (PR3)-ANCA. Results: Of the 74 patients with SLE evaluated, 60 (81.1%) of them were ANCA-positive by IIF. By contrast, only one patient showed specificity for PR3-ANCA by ELISA. The relevance of ANCA positivity by IIF and clinical and serological features was significant for renal involvement (p = .0174), and Systemic Lupus Erythematosus Disease Activity Index (SLEDAI) (p = .0308). Conclusion: ANCAs are common in the serum of patients with SLE, as detected by ethanol-fixed slides with IIF staining. However, detection of specificity to PR3 and/or MPO is rare, thus highlighting the importance of detecting these autoantibodies by different techniques.


RESUMEN Introducción: El lupus eritematoso sistémico (LES) es una enfermedad autoinmune sistémica. Los pacientes con LES muestran múltiples autoanticuerpos séricos, incluyendo los anticuerpos anticitoplasma de neutrófilo (ANCA, por sus siglas en inglés). Existen 2 técnicas principales para la detección de ANCA: inmunofluorescencia indirecta (IFI) y ensayo por inmunoadsorción ligado a enzimas (ELISA). En este estudio nuestro objetivo fue determinar la frecuencia y las asociaciones clínicas de los ANCA en pacientes con LES. Métodos: Realizamos un estudio transversal de 74 pacientes con LES en un hospital de alta complejidad de Colombia. La presencia de ANCA se evaluó por IFI, utilizando láminas con fijación de etanol, y con ELISA para determinar las especificidades para mieloperoxidasa (MPO)-ANCA y proteinasa 3 (PR3)-ANCA. Resultados: Fueron evaluados 74 pacientes con LES, 60 (81,1%) de ellos fueron positivos para ANCA. Por el contrario, solo un paciente mostró especificidad para PR3-ANCA por ELISA. La relación entre la positividad para ANCA por IFI y las características clínicas y serológicas fue estadísticamente significativa para compromiso renal (p = 0,0174) y para el índice de actividad de la enfermedad (Systemic Lupus Erythematosus Disease Activity Index [SLEDAI]) (p = 0,0308). Conclusiones: Los ANCA detectados mediante fijación con etanol por técnicas de IFI, son comunes en pacientes con LES. Sin embargo, la detección de especificidades para PR3 o MPO es rara; se destaca la importancia de la evaluación de estos autoanticuerpos mediante diferentes técnicas.


Assuntos
Humanos , Adulto , Imunoproteínas , Proteínas Sanguíneas , Doenças da Pele e do Tecido Conjuntivo , Doenças do Tecido Conjuntivo , Anticorpos Anticitoplasma de Neutrófilos , Aminoácidos, Peptídeos e Proteínas , Lúpus Eritematoso Sistêmico
7.
Rev. cientif. cienc. med ; 25(2): 125-132, 2022.
Artigo em Espanhol | LILACS | ID: biblio-1426805

RESUMO

Introducción: la discapacidad intelectual se considera un problema de salud pública global, la prevalencia oscila entre el 1% al 3% de la población mundial, cifra de la que se estima el origen genético estaría representado por el 5-7% de síndromes subteloméricos. El objetivo de la presente investigación fue determinar la frecuencia de discapacidad intelectual de etiología genética debida a rearreglos cromosómicos crípticos en 69 pacientes del IDAI. Material y Métodos. El estudio descriptivo de corte transversal se realizó en el Instituto de Genética en 69 pacientes con discapacidad intelectual de 5 a 18 años del Instituto de Adaptación Infantil (IDAI). El estudio fue dividido en tres etapas, la primera consistió en la elaboración de la historia clínica genética, seguidamente, se realizó el estudio de cariotipo en sangre periférica a todos los pacientes, finalmente, con la sospecha diagnóstica se realizó citogenética molecular a nueve de ellos, empleando una sonda locus específica. Resultados. Se encontró 43.48% de rearreglos cromosómicos, 24.67% correspondió a síndromes crípticos, de estos el 7.25% respondió a síndromes subteloméricos. Se observó mayor afectación en la población masculina: 45 hombres (65%) y 24 mujeres (35%), obteniendo una razón de sexo de 1.88 a favor del sexo masculino. Conclusiones. Se debe considerar la causa genética en toda discapacidad intelectual idiopática, sobre todo la debida a rearreglos cromosómicos crípticos . Para confirmar la sospecha diagnóstica se emplean técnicas de citogenética clásica y de hibridación fluorescente in situ , de esta manera se llega a un diagnóstico más preciso para coadyuvar en el asesoramiento genético del paciente.


Introduction. Intellectual disability is considered a global public health problem, the prevalence ranges from 1% to 3% of the world population, a figure whose genetic origin is estimated to be represented by 5-7% of subtelomeric syndromes. The objective of this research was to determine the frequency of intellectual disability of genetic etiology due to cryptic chromosomal rearrangements in 69 patients of IDAI. Material and methods. The descriptive cross-sectional study was carried out at the Institute of Genetics in 69 patients with intellectual disabilities from 5 to 18 years of age from the Institute for Child Adaptation (IDAI). The study was divided into three stages, the first consisted of preparing the genetic clinical history, then peripheral blood karyotyping was performed on all patients, finally, with suspected diagnosis, molecular cytogenetics was performed on nine of them, using a locus-specific probe. Results. 43.48% of chromosomal rearrangements were found, 24.67% corresponded to cryptic syndromes, of these 7.25% responded to subtelomeric syndromes. Greater involvement was observed in the male population: 45 men (65%) and 24 women (35%), obtaining a sex ratio of 1.88 in favor of the male sex. Conclusions. The genetic cause must be considered in all idiopathic intellectual disability, especially that due to cryptic chromosomal rearrangements. To confirm the diagnostic suspicion, classical cytogenetics and fluorescent in situ hybridization techniques are used, thus reaching a more precise diagnosis to assist in the genetic counseling of the patient.


Assuntos
Deficiência Intelectual , Hibridização in Situ Fluorescente
8.
Med. lab ; 26(3): 273-286, 2022. Tabs
Artigo em Espanhol | LILACS | ID: biblio-1412400

RESUMO

Introducción. La leucemia promielocítica aguda (LPA) es un subtipo poco frecuente de leucemia mieloide aguda (LMA), que se caracteriza por un comportamiento clínico particularmente agresivo, y en ausencia de tratamiento, su curso generalmente es fatal. El objetivo de este trabajo fue determinar las características clínicas y citogenéticas de una cohorte de pacientes con LPA, con la finalidad de evaluar su relación con las complicaciones, el pronóstico y el desenlace de estos pacientes. Metodología. Se realizó un estudio observacional, descriptivo, retrospectivo de los pacientes mayores de 15 años con diagnóstico de LPA, atendidos en el Hospital Universitario San Vicente Fundación, entre los años 2012 a 2020. Resultados. Un total de 32 pacientes fueron incluidos. La edad media del diagnóstico fue 37 años. El 84,4% de los pacientes tenía la traslocación (15;17) en el cariotipo, y el 93,75% tenían FISH positivo. El 12,5% de los casos tenían cariotipo complejo. La mortalidad en los primeros 30 días fue del 15,6%, siendo el sangrado la causa de muerte más frecuente. Todos los pacientes que sobrevivieron alcanzaron la remisión completa (84,3%). En un promedio de seguimiento de 24 meses, el 14,8% de los casos recayeron. En el análisis bivariado se encontró relación entre sexo masculino y tener cariotipo complejo (p=0,015). No se encontró relación entre cariotipo complejo y mortalidad temprana (p=0,358), tampoco entre cariotipo complejo y recaída (p=0,052). Conclusiones. Se presentan las características clínicas y citogenéticas de una cohorte de pacientes con LPA en Colombia. El sangrado en el sistema nervioso central fue la principal causa de mortalidad temprana, todos los pacientes que sobrevivieron alcanzaron la remisión completa con la terapia de inducción. Las tasas de mortalidad, remisión completa y recaída fueron similares a las reportadas por otras series latinoamericanas, pero inferiores a estudios provenientes de países europeos. Contrario a lo reportado en otros estudios, no se encontró relación entre el cariotipo complejo y la mortalidad temprana o recaída.


Introduction. Acute promyelocytic leukemia (APL) is a rare subtype of acute myeloid leukemia (AML), characterized by a particularly aggressive clinical behavior, that in the absence of treatment is usually fatal. The objective of this work was to determine the clinical and cytogenetic characteristics of a cohort of patients with APL, in order to evaluate their relationship with the outcome and prognosis of these patients. Methodology. An observational, descriptive, retrospective study of patients older than 15 years with a diagnosis of APL treated at the Hospital Universitario San Vicente Fundación, between 2012 and 2020, was carried out. Results. A total of 32 patients were included. The mean age at diagnosis was 37 years, 84.4% of the patients had the t(15;17) in the karyotype, and 93.75% had positive FISH. 12.5% of cases had a complex karyotype. Mortality in the first 30 days was 15.6%, with bleeding being the most common cause of death. All patients who survived achieved complete remission (84.3%). In an average follow-up of 24 months, 14.8% of cases relapsed. In the bivariate analysis, a relationship was found between the male sex and having a complex karyotype (p<0.015). No relationship was found between complex karyotype and early mortality (p=0.358), nor between complex karyotype and relapse (p=0.052). Conclusions. We present the clinical and cytogenetic characteristics of a cohort of patients with APL in Colombia. Central nervous system bleeding was the main cause of early mortality, with all surviving patients achieving complete remission on induction therapy. Mortality, complete remission and relapse rates were similar to those reported by other Latin American series, but lower than studies from European countries. Contrary to what has been reported in other studies, no relationship was found between complex karyotype and early mortality or relapse


Assuntos
Leucemia Promielocítica Aguda , Tretinoína , Idarubicina , Hibridização in Situ Fluorescente , Cariótipo , Trióxido de Arsênio
9.
Semina Ci. agr. ; 42(05): 2813-2824, set.-out. 2021. ilus, tab
Artigo em Inglês | VETINDEX | ID: vti-33449

RESUMO

The genus Brachyspira corresponds to the group of bacteria formerly classified into the genus Serpulina and includes several commensal and pathogenic intestinal spirochetes that affect pigs, poultry, and other animal species, including humans. In birds, some pathogenic species of this genus causes a condition known as avian intestinal spirochetosis, which remains under diagnosed, thereby causing serious economic losses. Brachyspira is a fastidious organism that necessitates the employment of fast and efficient identification techniques. The aim of this study was to identify Brachyspira spp. using histology, immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) in formalin-fixed paraffin embedded (FFPE) tissue samples from the cecum of commercial poultry. Samples were collected from129 birds aged between 35 and 45 days from commercial broiler farms. For evaluation, routine histology processing (H&E) and the histochemical technique, periodic acid–Schiff (PAS) were done. Additionally, FFPE tissue samples were evaluated for FISH and IHC. The histological lesions were analyzed and graded after H&E staining, and the goblet cells were counted and compared using PAS staining with the positive and negative samples obtained through FISH and IHC. For FISH, probes labeled with Brachyspira spp., B. pilosicoli, B. hyodysenteriae, and B. intermedia were used, where as rabbit polyclonal antibody specific for Brachyspira spp. was used for IHC. Of 129 samples, 82 were positive with IHC and 86 were positive with FISH. The samples positive for the genus Brachyspira in the FISH technique were tested for B. pilosicoli, B. hyodysenteriae, and B. intermedia in which 56 were positive for B. pilosicoli, 75 for B. hyodysenteriae and 80 for B. intermedia. There was an increase in goblet cells in the samples positive for FISH and IHC. The techniques used were effective and gave corresponding results, thus serving as a fast and efficient tool for diagnosis.(AU)


O gênero Brachyspira corresponde ao grupo de bactérias anteriormente classificadas no gênero Serpulinae inclui várias espiroquetas intestinais comensais e patogênicas que afetam suínos, aves e outras espécies animais, incluindo humanos. Em aves, algumas espécies patogênicas desse gênero causam uma condição conhecida como espiroquetose intestinal aviária, que permanece sub-diagnosticada, causando sérios prejuízos econômicos. Brachyspira é um organismo fastidioso que necessita do emprego de técnicas de identificação rápidas e eficientes. O objetivo deste estudo foi identificar Brachyspira spp. usando histologia, imunohistoquímica (IHQ) e hibridização fluorescente in situ (FISH) em amostras de tecido fixado em formalina e embebido em parafina (TFEP) do ceco de aves comerciais. As amostras foram coletadas de 129 aves com idades entre 35 e 45 dias em granjas comerciais. Para avaliação, o processamento histológico de rotina (H&E) e a técnica histoquímica, ácido periódico-Schiff (PAS) foram realizados. Além disso, as amostras de tecido TFEP foram avaliadas para FISH e IHC. As lesões histológicas foram analisadas e graduadas após coloração H&E, e as células caliciformes contadas e comparadas pela coloração PAS com as amostras positivas e negativas obtidas por FISH e IHC. Para FISH, foram utilizadas sondas marcadas com Brachyspira spp., B. pilosicoli, B. hyodysenteriae e B. intermedia, enquanto o anticorpo policlonal de coelho específico para Brachyspira spp. foi usado para IHC. De 129 amostras, 82 foram positivas com IHC e 86 foram positivas com FISH. As amostras positivas para o gênero Brachyspira pela técnica de FISH foram testadas para B. pilosicoli, B. hyodysenteriae e B. intermedia, sendo 56 positivas para B. pilosicoli, 75 para B. hyodysenteriae e 80 para B. intermedia. Houve aumento de células [...].(AU)


Assuntos
Animais , Galinhas/anatomia & histologia , Brachyspira/química , Brachyspira/imunologia , Infecções por Bactérias Gram-Negativas/diagnóstico , Infecções por Bactérias Gram-Negativas/imunologia , Infecções por Bactérias Gram-Negativas/patologia , Infecções por Bactérias Gram-Negativas/prevenção & controle , Infecções por Bactérias Gram-Negativas/veterinária
10.
Semina ciênc. agrar ; 42(05): 2813-2824, set.-out. 2021. ilus, tab
Artigo em Inglês | VETINDEX | ID: biblio-1501874

RESUMO

The genus Brachyspira corresponds to the group of bacteria formerly classified into the genus Serpulina and includes several commensal and pathogenic intestinal spirochetes that affect pigs, poultry, and other animal species, including humans. In birds, some pathogenic species of this genus causes a condition known as avian intestinal spirochetosis, which remains under diagnosed, thereby causing serious economic losses. Brachyspira is a fastidious organism that necessitates the employment of fast and efficient identification techniques. The aim of this study was to identify Brachyspira spp. using histology, immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) in formalin-fixed paraffin embedded (FFPE) tissue samples from the cecum of commercial poultry. Samples were collected from129 birds aged between 35 and 45 days from commercial broiler farms. For evaluation, routine histology processing (H&E) and the histochemical technique, periodic acid–Schiff (PAS) were done. Additionally, FFPE tissue samples were evaluated for FISH and IHC. The histological lesions were analyzed and graded after H&E staining, and the goblet cells were counted and compared using PAS staining with the positive and negative samples obtained through FISH and IHC. For FISH, probes labeled with Brachyspira spp., B. pilosicoli, B. hyodysenteriae, and B. intermedia were used, where as rabbit polyclonal antibody specific for Brachyspira spp. was used for IHC. Of 129 samples, 82 were positive with IHC and 86 were positive with FISH. The samples positive for the genus Brachyspira in the FISH technique were tested for B. pilosicoli, B. hyodysenteriae, and B. intermedia in which 56 were positive for B. pilosicoli, 75 for B. hyodysenteriae and 80 for B. intermedia. There was an increase in goblet cells in the samples positive for FISH and IHC. The techniques used were effective and gave corresponding results, thus serving as a fast and efficient tool for diagnosis.


O gênero Brachyspira corresponde ao grupo de bactérias anteriormente classificadas no gênero Serpulinae inclui várias espiroquetas intestinais comensais e patogênicas que afetam suínos, aves e outras espécies animais, incluindo humanos. Em aves, algumas espécies patogênicas desse gênero causam uma condição conhecida como espiroquetose intestinal aviária, que permanece sub-diagnosticada, causando sérios prejuízos econômicos. Brachyspira é um organismo fastidioso que necessita do emprego de técnicas de identificação rápidas e eficientes. O objetivo deste estudo foi identificar Brachyspira spp. usando histologia, imunohistoquímica (IHQ) e hibridização fluorescente in situ (FISH) em amostras de tecido fixado em formalina e embebido em parafina (TFEP) do ceco de aves comerciais. As amostras foram coletadas de 129 aves com idades entre 35 e 45 dias em granjas comerciais. Para avaliação, o processamento histológico de rotina (H&E) e a técnica histoquímica, ácido periódico-Schiff (PAS) foram realizados. Além disso, as amostras de tecido TFEP foram avaliadas para FISH e IHC. As lesões histológicas foram analisadas e graduadas após coloração H&E, e as células caliciformes contadas e comparadas pela coloração PAS com as amostras positivas e negativas obtidas por FISH e IHC. Para FISH, foram utilizadas sondas marcadas com Brachyspira spp., B. pilosicoli, B. hyodysenteriae e B. intermedia, enquanto o anticorpo policlonal de coelho específico para Brachyspira spp. foi usado para IHC. De 129 amostras, 82 foram positivas com IHC e 86 foram positivas com FISH. As amostras positivas para o gênero Brachyspira pela técnica de FISH foram testadas para B. pilosicoli, B. hyodysenteriae e B. intermedia, sendo 56 positivas para B. pilosicoli, 75 para B. hyodysenteriae e 80 para B. intermedia. Houve aumento de células [...].


Assuntos
Animais , Brachyspira/imunologia , Brachyspira/química , Galinhas/anatomia & histologia , Infecções por Bactérias Gram-Negativas/diagnóstico , Infecções por Bactérias Gram-Negativas/imunologia , Infecções por Bactérias Gram-Negativas/patologia , Infecções por Bactérias Gram-Negativas/prevenção & controle , Infecções por Bactérias Gram-Negativas/veterinária
11.
Biota Neotrop. (Online, Ed. ingl.) ; 21(2): e20201130, 2021. tab, graf
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-1249074

RESUMO

Abstract: The population structure of the longsnout seahorse Hippocampus reidi (Ginsburg 1933) was evaluated in an estuarine system in northeastern Brazil. A total of 425 captures were performed in the Vaza-Barris estuary and 232 individuals were marked with Visible Implant Fluorescent Elastomers. The population was estimated to be composed of 428 fish. The von Bertalanffy growth curve shows a steeper growth during the first months of life, up to about 17 months, and stabilization after 16 cm and 27 months of age. The hereby study indicates that the Vaza-Barris estuary is home to resident populations of longsnout seahorses. Moreover, these populations use mangrove roots as the main substrate for attachment and present well-defined ecological characteristics, such as habitat fidelity and highly structured social organization.


Resumo: A estrutura populacional do cavalo-marinho Hippocampus reidi (Ginsburg 1933) foi avaliada em um sistema estuarino no nordeste do Brasil. Um total de 425 capturas foram realizadas no estuário de Vaza-Barris e 232 indivíduos foram marcados com Elastômeros Fluorescentes de Implante Visível. A população foi estimada em 428 peixes. A curva de crescimento de von Bertalanffy mostra um crescimento mais acentuado durante os primeiros meses de vida, até cerca de 17 meses, e estabilização após os 16 cm e 27 meses de idade. O presente estudo indica que o estuário Vaza-Barris é o lar de populações residentes de cavalos-marinhos de focinho comprido. Além disso, essas populações utilizam raízes de mangue como principal substrato de fixação e apresentam características ecológicas bem definidas, como fidelidade de habitat e organização social altamente estruturada.

12.
Rev. MVZ Córdoba ; 25(3): 80-88, sep.-dic. 2020. tab, graf
Artigo em Espanhol | LILACS-Express | LILACS | ID: biblio-1394664

RESUMO

RESUMEN Objetivo. Estimar la seroprevalencia a Neospora caninum en caninos del área urbana y rural de Cumaral, Meta y determinar algunos factores de riesgo asociados a la seropositividad. Materiales y métodos. Se efectuó un estudio transversal en 222 perros (112 perros del área urbana y 110 del área rural). El tamaño de la muestra fue calculado en el programa Epidat v. 3.1. Los sueros sanguíneos fueron analizados mediante la técnica de Inmunofluorescencia Indirecta para IgG con un kit comercial. Los análisis de frecuencias, chi-cuadrado, fueron realizados mediante el paquete estadístico SPSS v. 25.0 Resultados. La seroprevalencia general fue 36.9% (IC95%: 30.9-43.5 %). La seropositividad entre los grupos fue: urbana (38.4 %) y rural (35.5%) (p>0.05), machos (36.9%) y hembras (36.9%) (p >0.05); en cachorros (32.7%), jóvenes (40.0%) y adultos (37.4%) (p>0.05), en contacto con predios pecuarios (40.7%) y sin contacto (35.2%) (p>0.05). Conclusiones. La seroprevalencia observada fue alta en las dos poblaciones analizadas y sugiere que los caninos han estado en contacto con el parásito, posiblemente por diferentes fuentes de infección que requieren ser estudiadas posteriormente.


ABSTRACT Objective. To estimate the seroprevalence to Neospora caninum in canines of the urban and rural area of Cumaral, Meta and determine some risk factors associated with seropositivity. Materials and methods. A cross-sectional study was carried out in 222 dogs (112 dogs from the urban area and 110 dogs from the rural area), the sample size was calculated by using Epidat v program. 3.1. The sera were analyzed using the Indirect Immunofluorescence technique for IgG with a commercial kit. Frequency analyzes by chi-square of independence were performed in SPSS v. 25.0 Results. The general seroprevalence was 36.9% (95% CI: 30.9-43.5%). The seropositivity between the groups was: urban (38.4%) and rural (35.5%) (p>0.05), males (36.9%) and females (36.9%) (p>0.05); in puppies (32.7%), youth (40.0%) and adults (37.4%) (p>0.05), in contact with livestock farms (40.7%) and without contact (35.2 %) (p>0.05), Conclusions. The seroprevalence observed was high in the two populations analyzed and suggests that the canines have been in contact with the parasite, possibly due to different sources of infection that need to be studied later.

13.
Gac. méd. boliv ; 43(2): 120-126, dic. 2020. ilus
Artigo em Espanhol | LILACS | ID: biblio-1249991

RESUMO

En diferentes regiones de Latinoamérica la infección por T. cruzi y Leishmania se superponen, por lo cual se reportan infecciones mixtas circulantes, debido a esto; deben realizarse pruebas diagnósticas específicas para evitar reacciones cruzadas entre estas dos patologías. OBJETIVO: determinar patrones de fluorescencia que permitan la diferenciación entre Leishmaniasis, enfermedad de Chagas e infección mixta empleando epimastigotes de T. cruzi. MÉTODOS: se empleó la técnica de Inmunofluorescencia Indirecta utilizando epimastigotes de T. cruzi (TcV autóctono) como antígeno figurado frente a un panel de muestras de suero codificados como A, B, C y D correspondientes a pacientes con infección por: Leishmaniasis (A), Infección mixta por Leishmania y Chagas(B), Enfermedad de Chagas (C) y sin ninguna de las dos infecciones (D). RESULTADOS: en los cuatro paneles de muestras se observaron diferentes patrones de intensidad de fluorescencia a nivel de membrana y núcleo de los epimastigotes de T. cruzi (TcV autóctono). CONCLUSIONES: la técnica de Inmunofluorescencia (IFI) con antígenos de epimastigotes de T. cruzi a demostrado utilidad en la diferenciación entre enfermedad de Chagas, Leishmaniasis y/o infecciones mixtas por ambos parásitos en aquellas zonas donde la coexistencia de ambas es habitual


In different regions of Latin America, infection by T. cruzi and Leishmania overlap, for which mixed circulating infections are reported, due to this; Specific diagnostic tests must be performed to avoid cross reactions between these two pathologies. OBJECTIVE: to determine fluorescence patterns that allow the differentiation between Leishmaniasis, Chagas disease and mixed infection using T. cruzi epimastigotes. METHODS: the Indirect Immunofluorescence technique was used using epimastigotes of T. cruzi (autochthonous TcV) as figurative antigen against a panel of serum samples coded as A, B, C and D corresponding to patients with infection by: Leishmaniasis (A) , Mixed infection by Leishmania and Chagas (B), Chagas disease (C) and without either of the two infections (D). RESULTS: in the four sample panels, different patterns of fluorescence intensity were observed at the membrane and nucleus level of the epimastigotes of T. cruzi (autochthonous TcV). CCONCLUSIONS: the Immunofluorescence technique (IFI) with T. cruzi epimastigote antigens has proven useful in differentiating between Chagas disease, Leishmaniasis and / or mixed infections by both parasites in areas where the coexistence of both is common.


Assuntos
Humanos , Trypanosoma cruzi , Leishmaniose , Fluorescência , Parasitos , Doença de Chagas , Infecções
14.
Rev. argent. cir ; 112(4): 508-516, dic. 2020. graf, il, tab
Artigo em Espanhol | LILACS, BINACIS | ID: biblio-1288163

RESUMO

RESUMEN Antecedentes: la fístula anastomótica (FA) es una de las complicaciones más severas de la cirugía colo rrectal. Su desarrollo obedece a múltiples factores dependientes del paciente y la técnica operatoria; entre estos últimos podemos mencionar la vascularización de los cabos como uno de los principales. La angiografía fluorescente (AF) permite la evaluación de ese factor en tiempo real durante la cirugía. Objetivo: describir la experiencia en el uso de la AF durante la cirugía colorrectal en un Hospital Uni versitario de la ciudad de Buenos Aires, registrar el cambio de conducta quirúrgica inducido por el uso de la AF y la incidencia de FA. Material y métodos: entre enero de 2016 y junio de 2018 se incluyeron 37 pacientes sometidos a resecciones colónicas programadas y con evaluación de la perfusión con AF. Resultados: la AF pudo realizarse en todos los pacientes con un tiempo operatorio extra de 3 a 9 mi nutos, registrando un cambio de conducta en 4 (10,81%) pacientes. Se registraron 2 (5,41%) fístulas anastomóticas. Conclusiones: la AF con verde de indocianina durante la cirugía colorrectal fue factible, sumó mínima complejidad y tiempo a la operación tradicional, y permitió cambiar la conducta en la sección intestinal en el 11% de los casos, con baja tasa de fístulas anastomóticas.


ABSTRACT Background: Anastomotic leak (AL) is one of the most feared complication of colorectal surgery. Its development is dependent of patient and surgery factors. Of these, bowel perfusion is one of the most important. Fluorescent angiography (FA) allows the identification in real time of the bowel perfusion. Objective: to describe the experience in the use of FA during colorectal surgery in a University Hospital setting in Buenos Aires, to register the change in surgical plan according to the FA findings and record the incidence of AL. Material y methods: Between January 2016 and June 2018, 37 patients with scheduled colorectal resections and FA bowel perfusion assessment were included. Results: Perfusion assessment with FA was possible in all cases with an extra operative time of 3 to 9 minutes, a change in surgical plan was registered in 4 cases (10.81%) and 2 AL were recorded (5.41%). Conclusions: FA during colorectal surgery is feasible, with minimum extra operative time and results in a change in surgical plan in 11% of the cases, and low rate of anastomotic fistula.


Assuntos
Humanos , Masculino , Feminino , Pessoa de Meia-Idade , Idoso , Angiofluoresceinografia , Neoplasias Colorretais/cirurgia , Colo/cirurgia , Colectomia , Cirurgia Colorretal , Fístula Anastomótica
15.
Biomédica (Bogotá) ; Biomédica (Bogotá);40(supl.2): 148-158, oct. 2020. graf
Artigo em Espanhol | LILACS | ID: biblio-1142458

RESUMO

Introducción. El nuevo coronavirus causante de un brote de enfermedad respiratoria aguda en China en diciembre de 2019 se identificó como SARS-CoV-2. La enfermedad, denominada COVID-19, fue declarada pandemia por la Organización Mundial de la Salud (OMS). El primer caso de COVID-19 en Colombia se reportó el 6 de marzo de 2020; en este estudio se caracterizó un aislamiento temprano del virus SARS-CoV-2 de una muestra recolectada en abril de 2020. Objetivos. Describir y caracterizar una cepa temprana a partir de un aislamiento de SARS-CoV-2 durante la pandemia en Colombia. Materiales y métodos. Se obtuvo una muestra de un paciente con COVID-19 confirmada por qRT-PCR; la muestra fue inoculada en diferentes líneas celulares hasta la aparición del efecto citopático. Para confirmar la presencia de SARS-CoV-2 en el cultivo, se utilizó la qRT-PCR a partir de los sobrenadantes, la inmunofluorescencia indirecta (IFI) en células Vero-E6, así como microscopía electrónica y secuenciación de nueva generación (next-generation sequencing). Resultados. Se confirmó el aislamiento de SARS-CoV-2 en células Vero-E6 por la aparición del efecto citopático tres días después de la infección, así como mediante la qRT-PCR y la IFI positiva con suero de paciente convaleciente positivo para SARS-CoV-2. Además, en las imágenes de microscopía electrónica de trasmisión y de barrido de células infectadas se observaron estructuras compatibles con viriones de SARS-CoV-2. Por último, se obtuvo la secuencia completa del genoma, lo que permitió clasificar el aislamiento como linaje B.1.5. Conclusiones. La evidencia presentada en este artículo permite confirmar el primer aislamiento de SARS-CoV-2 en Colombia. Además, muestra que esta cepa se comporta en cultivo celular de manera similar a lo reportado en la literatura para otros aislamientos y que su composición genética está acorde con la variante predominante en el mundo. Finalmente, se resalta la importancia que tiene el aislamiento viral para la detección de anticuerpos, para la caracterización genotípica y fenotípica de la cepa y para probar compuestos con potencial antiviral.


Introduction: SARS-CoV-2 has been identified as the new coronavirus causing an outbreak of acute respiratory disease in China in December, 2019. This disease, currently named COVID-19, has been declared as a pandemic by the World Health Organization (WHO). The first case of COVID-19 in Colombia was reported on March 6, 2020. Here we characterize an early SARS-CoV-2 isolate from the pandemic recovered in April, 2020. Objective: To describe the isolation and characterization of an early SARS-CoV-2 isolate from the epidemic in Colombia. Materials and methods: A nasopharyngeal specimen from a COVID-19 positive patient was inoculated on different cell lines. To confirm the presence of SARS-CoV-2 on cultures we used qRT-PCR, indirect immunofluorescence assay, transmission and scanning electron microscopy, and next-generation sequencing. Results: We determined the isolation of SARS-CoV-2 in Vero-E6 cells by the appearance of the cytopathic effect three days post-infection and confirmed it by the positive results in the qRT-PCR and the immunofluorescence with convalescent serum. Transmission and scanning electron microscopy images obtained from infected cells showed the presence of structures compatible with SARS-CoV-2. Finally, a complete genome sequence obtained by next-generation sequencing allowed classifying the isolate as B.1.5 lineage. Conclusion: The evidence presented in this article confirms the first isolation of SARS-CoV-2 in Colombia. In addition, it shows that this strain behaves in cell culture in a similar way to that reported in the literature for other isolates and that its genetic composition is consistent with the predominant variant in the world. Finally, points out the importance of viral isolation for the detection of neutralizing antibodies, for the genotypic and phenotypic characterization of the strain and for testing compounds with antiviral potential.


Assuntos
Infecções por Coronavirus , Microscopia Eletrônica , Técnica Indireta de Fluorescência para Anticorpo , Síndrome Respiratória Aguda Grave , Coronavírus Relacionado à Síndrome Respiratória Aguda Grave , Sequenciamento de Nucleotídeos em Larga Escala
16.
Rev. cuba. pediatr ; 92(2): e822, abr.-jun. 2020. tab
Artigo em Espanhol | LILACS, CUMED | ID: biblio-1126743

RESUMO

Introducción: El diagnóstico prenatal mediante la hibridación fluorescente in situ disminuye el tiempo de diagnóstico al no ser necesario el cultivo celular. Objetivo: Describir las características y experiencias del diagnóstico prenatal por hibridación fluorescente in situ en Cuba. Método: En amniocitos in situ se aplicaron sondas CEP y LSI para la detección de aneuploidías de los cromosomas 21,18,13, X y Y y sondas LSI para la detección de deleciones asociadas a síndromes de microdeleción. Resultados: Se remitieron al Centro Nacional de Genética Médica 629 casos de alto riesgo genético. Prevaleció la indicación de alteraciones fetales detectadas por ecografía. En 612 (97 por ciento) casos se obtuvo un diagnóstico satisfactorio, entre ellos, 50 (8,1 por ciento) casos positivos, con predominio del síndrome Down en 26 casos. Se corroboraron por citogenética convencional 312 casos con 98 por ciento de concordancia con los resultados obtenidos por hibridación fluorescente in situ. Se utilizó el líquido amniótico refrigerado para corroborar casos de diagnóstico dudoso obtenido por citogenética y se detectaron 3 fetos con mosaicos cromosómicos, el origen de un cromosoma marcador y la definición del sexo fetal en un caso. Conclusiones: Con la tecnología por hibridación fluorescente in situ el diagnóstico prenatal logra una segura opción de análisis en aquellos casos de embarazos de alto riesgo genético. Debido a limitaciones tecnológicas, la prueba por hibridación fluorescente in situ en células amnióticas en interfase, se ha adaptado a nuestras condiciones, para lograr siempre un diagnóstico seguro con el menor perjuicio posible a la embarazada, el feto y su familia(AU)


Introduction: Prenatal diagnosis by fluorescent in situ hybridization decreases the time of diagnosis not being necessary the cell culture. Objective: To describe the characteristics and experiences of prenatal diagnosis by fluorescent in situ hybridization in Cuba. Method: In in situ amniocytes CEP catheters were applied and LSI for the detection of aneuploidies of the 21,18,13, X and Y chromosomes, and LSI catheters for the detection of deletions associated with microdeletion syndromes. Results: 629 cases of high genetic risk were referred to the National Center of Medical Genetics. There was a prevalence of the indication of fetal abnormalities detected by ultrasound. In 612 (97 percent) cases the diagnosis was achieved in a satisfactory form, among them 50 (8.1 percent) positive cases, with predominance of Down syndrome in 26 cases. There were corroborated 312 cases by conventional cytogenetics with 98 percent of agreement with the results obtained by fluorescent in situ hybridization. It was used the cooled amniotic fluid to corroborate cases of uncertain diagnosis obtained by cytogenetics and there were detected 3 fetuses with chromosomal mosaics, the origin of a marker chromosome and the definition of fetal sex in one case. Conclusions: With the technology by fluorescent in situ hybridization, the prenatal diagnosis achieved a safe analysis option in cases of genetic high-risk pregnancies. Due to technological limitations, the test by fluorescent in situ hybridization in amniotic cells in interphase has adapted to the conditions in order to always achieve a safe diagnosis with the less possible damage to the pregnant women, the fetus and its family(AU)


Assuntos
Humanos , Feminino , Gravidez , Diagnóstico Pré-Natal/métodos , Hibridização in Situ Fluorescente/métodos , Epidemiologia Descritiva , Estudos Retrospectivos
17.
Rev. cuba. pediatr ; 92(2): e938, abr.-jun. 2020. tab, graf
Artigo em Espanhol | CUMED, LILACS | ID: biblio-1126751

RESUMO

Introducción: El lavado de manos deficiente, sigue siendo un problema hospitalario. Cumplir con las recomendaciones internacionales ofrece seguridad en el cuidado. Objetivo: Identificar el nivel de conocimiento, actitudes y prácticas en la técnica del lavado de manos en personal de salud en un hospital pediátrico de La Habana. Metodología: Estudio descriptivo a 145 trabajadores (31,7 por ciento médicos, 35,2 por ciento enfermeros, 22,8 por ciento médicos residentes y 10,3 por ciento estudiantes). De 13 servicios el cuerpo de guardia, sala de respiratorio, cirugía, neurocirugía y quemados, fueron los que más participación tuvieron. El estudio se realizó en tres etapas: 1. Observación directa de la técnica del lavado de manos. 2. Observación de la calidad del lavado de manos mediante lámpara fluorescente. 3. Medida del conocimiento acerca del lavado de manos y actitud mediante encuesta. Resultados: En la primera etapa, el 51,7 por ciento calificó de inadecuado, en la segunda etapa el 64,8 por ciento también tuvo una evaluación inadecuada y en las encuestas, el conocimiento alcanzó solo 52,4 por ciento, considerado inapropiado. La actitud ante esta actividad fue calificada de inadecuado en el 53,1 por ciento, con un mejor porcentaje en el personal de enfermería. Conclusiones: Los conocimientos, actitudes y prácticas sobre el lavado de manos es deficiente, por lo que debe diseñarse un programa de capacitación en todos los niveles, sea pregrado, posgrado o formación continua, donde exista responsabilidad individual de cada profesional sanitario, y se insista en ello(AU)


Introduction: Deficient handwashing is still a problem in hospitals. The compliance of the international recommendations on this matter provides security in the care. Objective: To identify the level of knowledge, attitudes and practices in the technique of handwashing in a pediatric hospital in Havana. Methodology: Descriptive study applied to 145 workers (31.7 percent doctors, 35.2 percent nurses, 22.8 percent residents, and 10.3 percent students) of 13 services. Emergencies, Respiratory, Surgery, Neurosurgery and Caumatology were the services with more participation. The study was conducted in three stages: 1. Direct observation of the technique of handwashing. 2. Quality observation of the handwashing using fluorescent lamp. 3. Extent of knowledge on handwashing and attitudes through survey. Results: In the first stage, the 51.7 percent the handwashing was assessed as inappropriate, in the second stage the 64.8 percent was also evaluated as inadequate, and in the surveys, the 52.4 percent had an incorrect knowledge; the attitude towards this activity was described as inappropriate in the 53.1 percent , with a better percentage in the nursing staff. Conclusions: The knowledge, attitudes and practices on handwashing were poor, so it must be designed a training program at all levels, whether undergraduate, graduate or continuing training, where individual responsibility of each healthcare professional exists, and should be highlithed(AU)


Assuntos
Desinfecção das Mãos/métodos , Conhecimentos, Atitudes e Prática em Saúde , Higiene das Mãos/métodos , Educação Médica/ética
18.
Rev. méd. hered ; 31(2): 95-100, abr.-jun 2020. tab
Artigo em Espanhol | LILACS-Express | LILACS | ID: biblio-1144822

RESUMO

Resumen Objetivo: Determinar la frecuencia de anticuerpos antimitocondriales y de anticuerpos contra antígenos extraíbles del núcleo en pacientes con cirrosis biliar primaria. Material y métodos: Estudio de tipo cuantitativo, observacional y transversal, realizado en el Servicio de Inmunología del Hospital Nacional Arzobispo Loayza entre enero 2018 y marzo 2019. Se revisaron las historias clínicas de 30 pacientes con características presuntivas de cirrosis biliar primaria; para la detección de los anticuerpos antinucleares y anticuerpos antimitocondriales se empleó el kit inmunológico en sangre y observación con microscopio de inmunofluorescencia a 40X y para la detección de los anticuerpos contra antígenos extraíbles del núcleo se empleó el método Immunoblot. Resultados: Se estudiaron 30 pacientes con cirrosis biliar primaria, 20 fueron de sexo femenino (66,7%). El patrón de tinción más frecuente fue el citoplasmático moteado reticular en 17(56,7%), seguido del patrón citoplasmático moteado reticular y patrón moteado en 7(23,3%) pacientes, y en menor frecuencia el patrón citoplasmático moteado reticular y patrón centromérico. Nueve (42,9%) pacientes con cirrosis biliar primaria tenían anti-M2. Se demostró mayor frecuencia, 21(70%) de los pacientes con cirrosis biliar primaria tenían anticuerpos antimitocondriales. Conclusiones: Se encontró alta frecuencia de patrón citoplasmático moteado reticular en pacientes con cirrosis biliar primaria, se demostró asociación significativa con los anti-M2 y anticuerpos antimitocondriales.


Summary Objective: To determine the frequency of antimitochondrial antibodies and antibodies against extractable nucleus antigens in patients with primary biliary cirrhosis. Methods : A quantitative, observational and cross-sectional study was carried out at the Immunology Service of the Arzobispo Loayza National Hospital between January 2018 and March 2019. The medical records of 30 patients with presumptive characteristics of primary biliary cirrhosis were reviewed; for the detection of the antinuclear antibodies and antimitochondrial antibodies, the immunological kit was used in blood and observation with a 40X immunofluorescence microscope, and the Immunoblot method was used for the detection of the antibodies against extractable nucleus antigens. Results: Thirty patients with primary biliary cirrhosis disease were studied, 20 were female (66.7%). The most frequent staining pattern was the reticular mottled cytoplasmic in 17 (56.7%), followed by the reticular mottled cytoplasmic pattern and mottled pattern in 7 (23.3%) patients, and less frequently the reticular mottled cytoplasmic pattern and centromeric. Nine (42.9%) patients with primary biliary cirrhosis had anti-M2. In the present investigation, a higher frequency was demonstrated, 21 (70%) of the patients with primary biliary cirrhosis had antimitochondrial antibodies. Conclusions: A high frequency of reticular mottled cytoplasmic pattern was found in patients with primary biliary cirrhosis; a significant association with anti-M2 and antimitochondrial antibodies was demonstrated.

19.
Rev. Asoc. Colomb. Cien. Biol. (En línea) ; 1(32): 145-153, 20200000. tab
Artigo em Espanhol | LILACS, COLNAL | ID: biblio-1379202

RESUMO

Objetivo: Correlacionar los niveles de anticuerpos antinucleares obtenidos por las técnicas de inmunofluorescencia indirecta (FANA) e inmunoensayo lineal (ANA-LIA) en pacientes diagnosticados o con sospecha clínica de enfermedad autoinmune. Métodos: Se incluyeron 100 pacientes que dieron su consentimiento informado para participar en el estudio. Los ensayos e interpretación de los resultados de las pruebas de FANA y ANA-LIA se realizaron siguiendo las recomendaciones del fabricante. Resultados: el 97,4% de los pacientes estudiados eran de sexo femenino con edad promedio de 42 años, siendo la Artritis reumatoide, Poliartritis y Lupus Eritematoso Sistémico las enfermedades que con mayor frecuencia acompañaron la solicitud médica. Se observó que ambos métodos fueron positivos en el 21% de los casos y que el indice de correlaciòn de Kappa entre las pruebas fue moderado (k= 0,51; p< 0,05), la sensibilidad y especificidad de los métodos fue del 71,4% y 84,8% respectivamente, se observó también que para un determinado patrón fluorescente puede haber positividad de más de un antígeno de la prueba de ANA-LIA y viceversa. Conclusiones: FANA es el método de tamizaje aceptado en la práctica clínica para orientar hacia un diagnóstico clínico de enfermedad autoinmune más probable, debido a la subjetividad en la interpretación de sus resultados y necesidad de entrenamiento en la identificación de los patrones fluorescentes, se requiere del apoyo de otros métodos de laboratorio que permitan identificar con mayor precisión los antígenos reconocidos por los autoanticuerpos, ANA-LIA es una herramienta de laboratorio costo-efectiva de elevada sensibilidad y especificidad que se ajusta a este requerimiento.


Objective: To correlate levels of antinuclear antibodies obtained by indirect immunofluorescence techniques (FANA) and linear immunoassay (ANA-LIA) in patients diagnosed or with clinical suspicion of autoimmune disease. Methods: 100 patients who gave their informed consent to participate in the study were included. Assays and interpretation of results of FANA and ANA-LIA test were performed following the manufacturer's recommendations. Results: 97,4% of the patients studied were female with an average age of 42 years, being rheumatoid arthritis, polyarthritis and systemic lupus erythematosus the diseases that most frequently accompanied the medical request. It was observed that both methods were positive in 21% of the cases and that the Kappa correlation index was moderate between the tests (k = 0,51; p <0,05), the sensitivity and specificity of the methods was 71,4% and 84,8% respectively. It was also observed that for a given fluorescent pattern there may be positivity of more than one antigen of the ANA-LIA test and vice versa. Conclusions: FANA is the screening method accepted in the clinical practice to guide towards a more probable clinical diagnosis of autoimmune disease. Due to the subjectivity in the interpretation of its results and the need for training in the identification of fluorescent patterns, the support of other laboratory methods that allow the identification of antigens recognized by autoantibodies with greater precision is necessary, ANA-LIA is a cost-effective laboratory tool of high sensitivity and specificity that meets this requirement.


Assuntos
Humanos , Anticorpos Antinucleares , Biomarcadores , Doenças Reumáticas
20.
Iatreia ; Iatreia;32(3): 167-176, Jul-Set. 2019. tab, graf
Artigo em Espanhol | LILACS | ID: biblio-1039996

RESUMO

RESUMEN Objetivo: determinar la frecuencia de la infección y enfermedad por Rickettsia spp. del grupo de las fiebres manchadas en pacientes febriles del Urabá antioqueño, que asistieron a centros hospitalarios de la región. Métodos: se incluyeron en el estudio pacientes febriles provenientes de 9 instituciones de salud de la región del Urabá, los cuales fueron encuestados para determinar sus variables clínicas y demográficas. De estos pacientes se obtuvieron muestras de suero durante las fases aguda y convaleciente de la enfermedad. Para cada muestra se determinó la seropositividad (título ≥ 64) y su título de anticuerpos seriados dobles mediante inmunofluorescencia indirecta para IgG contra el antígeno de Rickettsia rickettsii. Resultados: se analizaron 89 pacientes febriles con 89 muestras de fase aguda y 60 en fase convaleciente. Los síntomas más comunes de los pacientes fueron cefalea, ictericia, mialgias, náuseas, dolor abdominal, trombocitopenia y vómito. El 55,1 % de los pacientes provenía de áreas rurales. Se obtuvo seropositividad del 40,4 % con títulos entre 64-512, infección previa en un 33,7 % y rickettsiosis en 6 pacientes (6,7 %). Los pacientes con seroconversión o serorefuerzo provenían de los municipios de Apartadó (n = 2), Chigorodó (n = 1), Necoclí (n = 2) y Turbo (n = 1); el hallazgo clínico más destacado fue la trombocitopenia. Conclusiones: se demostró que la infección y la enfermedad rickettsial continúan siendo activas en la zona del Urabá. Este hallazgo permite alertar a las autoridades de salud de la región para que se brinde tratamiento con antibióticos a los casos sospechosos de manera temprana y de esta forma evitar las muertes o secuelas derivadas de este tipo de infecciones.


SUMMARY Objective: Determine the frequency of infection and disease by Rickettsia spp. of the spotted fever group in febrile patients from Urabá Antioquia attended by hospital centers of the region. Methods: Patients from nine health institutions of the Urabá region were included in the study. These patients received a survey with questions about their clinical and socio-demographic variables. Eighty-nine acutephase serum samples, and 60 convalescent serum samples, were obtained from these patients, and each sample was tested (IgG) by Indirect Immunofluoerscence Assay (IIFA) using a dilution of 1:64 against R. rickettsii. Furtherly, positive sera were tittered by two-fold serial dilutions using the same antigen. Results: Patients showed symptoms such as fever, headache, jaundice, myalgias, nausea, abdominal pain, petechiae, thrombocytopenia and vomiting. Most of these patients came from rural areas (55,1 %). Seropositivity was obtained in 40,4 % patients with titers between 64-512, a 33,7 % with previous infection and the disease was found in 6 patients (6,7 %). Patients with seroconversion, or a fourlfold rise antibody titer between acute and convalescent samples, came from the municipalities of Apartadó (n = 2), Chigorodó (n = 1), Necoclí (n = 2) and Turbo (n = 1), and the most relevant clinical finding was thrombocytopenia in four of the patients. Conclusions: This study demonstrated that infection and rickettsial disease continues being active in the Urabá region. This situation represents a warning for the health authorities of the region and suggests them to provide appropriate treatment to avoid deaths or sequelae derived from this type of infections.


Assuntos
Humanos , Infecções por Rickettsia , Febre
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